Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants.

abstract：:It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...

journal_title：Virology

authors： Stanley J,Goldbach R,Van Kammen A

更新日期：1980-10-15 00:00:00

abstract：:Human papillomavirus type 16 (HPV-16) infections can in rare cases persist and cause lesions that may progress to cervical cancer. Cells in the lesions are not permissive for virus production, nor are cervical cancer cells. The intracellular environment is such that it prevents production of the highly immunogenic, vi...

journal_title：Virology

authors： Zhao X,Fay J,Lambkin H,Schwartz S

更新日期：2007-12-20 00:00:00

abstract：:We have examined the host range of AIDS-associated retroviruses (ARV) that are known to infect human T cells of the helper subset. We have observed that the virus cannot infect fibroblast and epithelial cell lines of many different animal species. It is infectious and replicates efficiently in peripheral mononuclear c...

journal_title：Virology

authors： Levy JA,Shimabukuro J,McHugh T,Casavant C,Stites D,Oshiro L

更新日期：1985-12-01 00:00:00

abstract：:Alternations of cellular gene expression following an adenovirus type 2 infection of human primary cells were studied by using superior sensitive cDNA sequencing. In total, 3791 cellular genes were identified as differentially expressed more than 2-fold. Genes involved in DNA replication, RNA transcription and cell cy...

journal_title：Virology

authors： Zhao H,Dahlö M,Isaksson A,Syvänen AC,Pettersson U

更新日期：2012-03-15 00:00:00

abstract：:Alphaviruses are mosquito-borne viruses that cause serious human and animal diseases. Previous studies demonstrated that a determinant within the nsP1/nsP2 cleavage domain of the virulent Sindbis AR86 virus played a key role in regulating adult mouse virulence without adversely affecting viral replication. Additional ...

journal_title：Virology

authors： Cruz CC,Suthar MS,Montgomery SA,Shabman R,Simmons J,Johnston RE,Morrison TE,Heise MT

更新日期：2010-03-30 00:00:00

abstract：:The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell...

journal_title：Virology

authors： Schowalter RM,Wurth MA,Aguilar HC,Lee B,Moncman CL,McCann RO,Dutch RE

更新日期：2006-07-05 00:00:00

abstract：:Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in con...

journal_title：Virology

authors： Kelleher CA,Paterson RK,Dreyfus DH,Streib JE,Xu JW,Takase K,Jones JF,Gelfand EW

更新日期：1995-04-20 00:00:00

abstract：:We reported earlier that variable expression of the Epstein-Barr virus (EBV) encoded membrane protein LMP 1 in nasopharyngeal carcinoma and the host-cell-phenotype-dependent activity of the BCR2 promoter (one of the possible initiator sites for transcripts of Epstein-Barr nuclear antigens) in Burkitt's lymphoma (BL) l...

journal_title：Virology

authors： Minarovits J,Hu LF,Minarovits-Kormuta S,Klein G,Ernberg I

更新日期：1994-05-01 00:00:00

abstract：:The P68 protein kinase (referred to as P68 based on its M(r) of 68,000 in human cells) is a serine/threonine kinase induced by interferon treatment and activated by dsRNAs. The kinase is under tight controls in virus-infected cells since once activated, it phosphorylates its natural substrate eukaryotic initiation fac...

journal_title：Virology

authors： Barber GN,Tomita J,Garfinkel MS,Meurs E,Hovanessian A,Katze MG

更新日期：1992-12-01 00:00:00

abstract：:Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12E1AB virus (containing E1A and E1B...

journal_title：Virology

authors： Sawada Y,Föhring B,Shenk TE,Raska K Jr

更新日期：1985-12-01 00:00:00

abstract：:While the 2009 pandemic H1N1 virus has become established in the human population as a seasonal influenza virus, continued adaptation may alter viral virulence. Here, we passaged a 2009 pandemic H1N1 virus (A/Changchun/01/2009) in mice. Serial passage in mice generated viral variants with increased virulence. Adapted ...

journal_title：Virology

authors： Yu Z,Cheng K,Sun W,Zhang X,Li Y,Wang T,Wang H,Zhang Q,Xin Y,Xue L,Zhang K,Huang J,Yang S,Qin C,Wilker PR,Yue D,Chen H,Gao Y,Xia X

更新日期：2015-12-01 00:00:00

abstract：:Hepatitis B virus synthesizes multiple spliced RNAs that can be reverse transcribed into viral DNA. We thoroughly characterized the contribution of spliced RNAs to DNA synthesis in transfected cultures of Huh7 and HepG2 cells. We found that up to 50% of DNA within intracellular capsids is derived from five spliced RNA...

journal_title：Virology

authors： Abraham TM,Lewellyn EB,Haines KM,Loeb DD

更新日期：2008-09-15 00:00:00

abstract：:While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...

journal_title：Virology

authors： Cristillo AD,Wang S,Caskey MS,Unangst T,Hocker L,He L,Hudacik L,Whitney S,Keen T,Chou TH,Shen S,Joshi S,Kalyanaraman VS,Nair B,Markham P,Lu S,Pal R

更新日期：2006-03-01 00:00:00

abstract：:Mouse hepatitis virus strain A59 (MHV-A59) encodes within the 22-kb gene 1 a large polyprotein containing three proteinase domains with proven or predicted cysteine catalytic residues. E64d, a specific, irreversible inhibitor of cysteine (thiol) proteinases, inhibits the processing of the gene 1 polyprotein. Specifica...

journal_title：Virology

authors： Kim JC,Spence RA,Currier PF,Lu X,Denison MR

更新日期：1995-04-01 00:00:00

abstract：:Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNA...

journal_title：Virology

authors： Scholthof KB,Scholthof HB,Jackson AO

更新日期：1995-08-01 00:00:00

abstract：:To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic ol...

journal_title：Virology

authors： Shanks M,Tomenius K,Clapham D,Huskisson NS,Barker PJ,Wilson IG,Maule AJ,Lomonossoff GP

更新日期：1989-12-01 00:00:00

abstract：:IL-17-producing (Th17) and regulatory T (Treg) cells have been well established in the pathogenesis of many inflammatory diseases. To assess whether Th17 and Treg were altered in acute virus-induced myocarditis (AVMC) mice, we assessed Th17/Treg functions on different levels in AVMC. It was shown that the expression o...

journal_title：Virology

authors： Xie Y,Chen R,Zhang X,Chen P,Liu X,Xie Y,Yu Y,Yang Y,Zou Y,Ge J,Chen H

更新日期：2011-12-05 00:00:00

abstract：:Significant economic losses in the poultry industries have resulted from H9N2 low pathogenic avian influenza virus infections across North Africa, the Middle East and Asia. The present study investigated the evolutionary dynamics of H9N2 viruses circulating in Korea from 1996 to 2012. Our analysis of viral population ...

journal_title：Virology

authors： Lee DH,Fusaro A,Song CS,Suarez DL,Swayne DE

更新日期：2016-01-15 00:00:00

abstract：:Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...

journal_title：Virology

authors： Volkman LE,Zaal KJ

更新日期：1990-03-01 00:00:00

abstract：:Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain E...

journal_title：Virology

authors： Wang X,Wu CX,Song XR,Chen HC,Liu ZF

更新日期：2017-06-01 00:00:00

abstract：:The (guanine-N7)-methyltransferase domain of the vaccinia virus mRNA capping enzyme is a heterodimer composed of a catalytic subunit D1(498-844) bound to a stimulatory subunit D12. To identify structural elements of the 287-amino-acid D12 subunit that participate in binding and activation of the catalytic subunit, we ...

journal_title：Virology

authors： Saha N,Shuman S

更新日期：2001-08-15 00:00:00

abstract：:The purified genomic dsRNA of bluetongue virus type 1 (BTV 1) was separated into 10 size classes by polyacrylamide gel electrophoresis. These genome segments were recovered individually from the gel, denatured with methylmercuric hydroxide and translated in vitro in a rabbit reticulocyte lysate system. The virus-speci...

journal_title：Virology

authors： Mertens PP,Brown F,Sangar DV

更新日期：1984-05-01 00:00:00

abstract：:By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and pa...

journal_title：Virology

authors： Hantz O,Baginski I,Fourel I,Chemin I,Trepo C

更新日期：1992-09-01 00:00:00

abstract：:Commelina yellow mottle virus (CoYMV) is the type member of the badnaviruses, a genus of plant pararetroviruses. The N-terminus of the polyprotein encoded by ORF III has limited similarity to known cell-to-cell movement proteins. To test the hypothesis that the N-terminus is required for viral movement, the phenotypes...

journal_title：Virology

authors： Tzafrir I,Ayala-Navarrete L,Lockhart BE,Olszewski NE

更新日期：1997-06-09 00:00:00

abstract：:As a simian species, the langurs are not known to harbor simian retroviruses, except for one report on a simian Type D endogenous retrovirus from the spectacled langur (Trachypithecus obscurus) from Malaysia. The present report describes for the first time natural infection of the common Hanuman langur (Semnopithecus ...

journal_title：Virology

authors： Nandi JS,Bhavalkar-Potdar V,Tikute S,Raut CG

更新日期：2000-11-10 00:00:00

abstract：:The complete nucleotide sequence of cloned cDNAs corresponding to the full length of the mRNA encoding the measles virus hemagglutinin (H) protein has been determined. the mRNA contains a single large open reading frame which is capable of encoding a protein of 617 amino acids with a molecular mass of 69,250 Da. The d...

journal_title：Virology

authors： Alkhatib G,Briedis DJ

更新日期：1986-04-30 00:00:00

abstract：:The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BKV exists as archetype virus and rearranged variants, w...

journal_title：Virology

authors： Broekema NM,Abend JR,Bennett SM,Butel JS,Vanchiere JA,Imperiale MJ

更新日期：2010-11-25 00:00:00

abstract：:Lentiviral vectors have gained much attention in recent years mainly because they integrate into nondividing host-cell genomes. For clinical applications, a safe and efficient lentiviral vector system is required. Previously, we have established a human immunodeficiency virus type 1 (HIV-1)-derived three-plasmid lenti...

journal_title：Virology

authors： Iwakuma T,Cui Y,Chang LJ

更新日期：1999-08-15 00:00:00

abstract：:The natural host of SIVrcm is the red-capped mangabey (Cercocebus torquatus torquatus). Although this virus infects macaques and human PBMCs, its pathogenic potential is unknown. We serially passaged SIVrcm through 9 rhesus macaques to assess its potential for virulence. SIVrcm infected all macaques with peak viremia ...

journal_title：Virology

authors： Ling B,Veazey RS,Marx PA

更新日期：2008-09-15 00:00:00

abstract：:The nucleotide sequence of the portion of naturally occurring defective EBV (HR-1) DNA (designated het DNA) which is responsible for disruption of EBV latency has been determined and compared with the regions of the standard HR-1 viral genome from which it was derived. This rearranged 2.7-kbp DNA fragment represented ...

journal_title：Virology

authors： Jenson HB,Miller G

更新日期：1988-08-01 00:00:00