Ligation of double-stranded and single-stranded [oligo(dT)] DNA by vaccinia virus DNA ligase.

abstract：:Multiple RNA species which hybridize to a cloned cDNA probe of the turnip crinkle virus satellite RNA (S-TCV) are found both in virions and in plants infected with virus particles containing S-TCV. At least six of these RNAs appear to be multimeric forms of the satellite RNA based on their migration in denaturing gels...

journal_title：Virology

authors： Altenbach SB,Howell SH

更新日期：1984-04-15 00:00:00

abstract：:Terminally redundant linear proviral DNA of approximately 9.5 kb was the major unintegrated species recovered in the Hirt supernatant fraction of caprine synovial membrane cells infected with strain 75-G63 caprine arthritis-encephalitis virus. A physical map based on the cleavage sites of 13 restriction endonucleases ...

journal_title：Virology

authors： Roberson SM,Cheevers WP

更新日期：1984-04-30 00:00:00

abstract：:Central African tropical forests are home to several species of non-human primates (NHPs), infected by Simian Immunodeficiency Virus (SIV). It is well-known that HIV-1 epidemic is due to cross-transmission and adaptation of SIV to humans. The main goal of this work was to investigate if a NHP bite is a risk factor for...

journal_title：Virology

authors： Filippone C,de Oliveira F,Betsem E,Schaeffer L,Fontanet A,Lemée V,Gessain A,Plantier JC

更新日期：2017-03-01 00:00:00

abstract：:A collection of TK+, ACV-resistant mutants of herpes simplex virus type 1 (HSV-1) has been derived using a selection system based on biochemically transformed cells. Evidence is presented suggesting that most of these mutants induce resistant DNA polymerase activities and are thus likely to express variant DNA polymer...

journal_title：Virology

authors： Larder BA,Darby G

更新日期：1985-10-30 00:00:00

abstract：:An infectious raccoon poxvirus (RCNV) was used to express the feline panleukopenia virus (FPV) open reading frame VP2. The recombinant, RCNV/FPV, was constructed by homologous recombination with a chimeric plasmid for inserting the expression cassette into the thymidine kinase (TK) locus of RCNV. Expression of the VP2...

journal_title：Virology

authors： Hu L,Esposito JJ,Scott FW

更新日期：1996-04-01 00:00:00

abstract：:Nineteen independent temperature-sensitive (ts) mutants were isolated from SA11 following mutagenesis with proflavin or 5-azacytidine. Fourteen of the ts mutants fell into one or another of five mutant groups previously defined by recombination. Five of the ts mutants defined five recombination groups (F, G, H, I, and...

journal_title：Virology

authors： Ramig RF

更新日期：1983-10-30 00:00:00

abstract：:The Hepatitis B virus precore protein is processed in the endoplasmic reticulum (ER) into secreted hepatitis B e antigen (HBeAg), which acts as an immune tolerogen to establish chronic infection. Downregulation of secreted HBeAg should improve clinical outcome, as patients who effectively respond to current treatments...

journal_title：Virology

authors： Walsh R,Nuttall S,Revill P,Colledge D,Cabuang L,Soppe S,Dolezal O,Griffiths K,Bartholomeusz A,Locarnini S

更新日期：2011-03-01 00:00:00

abstract：:Insertion of a functional splicing cassette into a construct containing the HIV-1 poly(A) site followed by the adenovirus L3 poly(A) site results in both specific stimulation of 3' end processing at the HIV-1 site and an increase in the steady-state levels of RNA processed at both sites. To further evaluate this influ...

journal_title：Virology

authors： Scott JM,Imperiale MJ

更新日期：1996-10-15 00:00:00

abstract：:The interferon (IFN) activity found in human leukocyte IFN alpha preparations, autoimmune and AIDS sera, and others was reported to have distinct antigenic and deviating biological properties. This led to its vague designation as acid-labile and thermolabile IFN alpha. However, using specific monoclonal antibodies, th...

journal_title：Virology

authors： Kontsek P,Borecký L,Novák M

更新日期：1991-03-01 00:00:00

abstract：:Rice yellow mottle virus (RYMV) is icosahedral in morphology and known to swell in vitro, but the biological function of swollen particles remains unknown. Anion-exchange chromatography was used to identify three markedly stable forms of RYMV particles from infected plants: (1) an unstable swollen form lacking Ca2+ an...

journal_title：Virology

authors： Brugidou C,Opalka N,Yeager M,Beachy RN,Fauquet C

更新日期：2002-05-25 00:00:00

abstract：:The nonstructural rotavirus receptor glycoprotein NS28 is 175 amino acids long and oriented in the RER membrane with the NH2 terminus on the luminal side and approximately 131 amino acids accessible from the cytoplasmic side. Au et al. (1988) have demonstrated that NS28 is able to interact with rotavirus single-shelle...

journal_title：Virology

authors： Meyer JC,Bergmann CC,Bellamy AR

更新日期：1989-07-01 00:00:00

abstract：:The sequence of Broadhaven (BRD) virus segment 5, the major genetic determinant of serotype, is 1658 nucleotides in length and contains a single open reading frame (ORF) having the coding capacity for a protein of Mr 52.5K. Comparison of the ORF of segment 5 of BRD virus with published sequences of bluetongue virus (B...

journal_title：Virology

authors： Moss SR,Fukusho A,Nuttall PA

更新日期：1990-11-01 00:00:00

abstract：:The porcine lymphotropic herpesviruses (PLHV) are discussed as possible risk factors in xenotransplantation because of the high prevalence of PLHV-1, PLHV-2 and PLHV-3 in pig populations world-wide and the fact that PLHV-1 has been found to be associated with porcine post-transplant lymphoproliferative disease. To pro...

journal_title：Virology

authors： Lindner I,Ehlers B,Noack S,Dural G,Yasmum N,Bauer C,Goltz M

更新日期：2007-01-20 00:00:00

abstract：:A non-plaque-forming vaccine strain of NDV (NDV-B1) interferes with the infection of cultured chick embryo cells by the virulent plaque-forming NDV-L strain. The level of interference increases when the concentration of interfering virus is increased or when the time between infection and superinfection is increased. ...

journal_title：Virology

authors： Bratt MA,Rubin H

更新日期：1967-12-01 00:00:00

abstract：:Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional...

journal_title：Virology

authors： Larson ET,Eilers BJ,Reiter D,Ortmann AC,Young MJ,Lawrence CM

更新日期：2007-07-05 00:00:00

abstract：:The tegument protein pp28 of human cytomegalovirus (HCMV) is essential for the assembly of infectious HCMV virions, but how it functions during the process of HCMV tegumentation and envelopment remains unclear. By using live cell fluorescence resonance energy transfer (FRET) microscopy and yeast two-hybrid assays, we ...

journal_title：Virology

authors： Liu Y,Cui Z,Zhang Z,Wei H,Zhou Y,Wang M,Zhang XE

更新日期：2009-05-25 00:00:00

abstract：:We have constructed chimeric papillomavirus-like particles (CVLPs) by replacing the 34-carboxy-terminal amino acids of the HPV 16 L1 protein with various parts of the HPV 16 E7 protein. Chimeric proteins were expressed by recombinant baculoviruses and analyzed by electron microscopy for their ability to assemble into ...

journal_title：Virology

authors： Müller M,Zhou J,Reed TD,Rittmüller C,Burger A,Gabelsberger J,Braspenning J,Gissmann L

更新日期：1997-07-21 00:00:00

abstract：:The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell...

journal_title：Virology

authors： Schowalter RM,Wurth MA,Aguilar HC,Lee B,Moncman CL,McCann RO,Dutch RE

更新日期：2006-07-05 00:00:00

abstract：:Coronaviruses have a marked tropism for epithelial cells. Entry and release of the porcine transmissible gastroenteritis virus (TGEV) is restricted to apical surfaces of polarized epithelial cells, as we have recently shown (J. W. A. Rossen, C. P. J. Bekker, W. F. Voorhout, G. J. A. M. Strous, A. van der Ende, and P. ...

journal_title：Virology

authors： Rossen JW,Voorhout WF,Horzinek MC,van der Ende A,Strous GJ,Rottier PJ

更新日期：1995-06-20 00:00:00

abstract：:Attachment of human adenoviruses (Ads) to host cells is mediated by the interaction of the fiber protein of the capsid with specific cell-surface molecules. For one of the species B adenoviruses, Ad3, the mechanism of binding to cells remains to be defined. Several previous reports have proposed CD46, CD80 or CD86 as ...

journal_title：Virology

authors： Hall K,Blair Zajdel ME,Blair GE

更新日期：2009-09-30 00:00:00

abstract：:The HIV-1 accessory gene product Vpr can inhibit cell proliferation via cell cycle arrest at the G(2) phase, and it can induce apoptosis after G(2) arrest. We found recently that C81, a carboxy-terminally truncated form of Vpr, induced apoptosis via G(1) arrest but did not induce G(2) arrest of the cell cycle. Thus, i...

journal_title：Virology

authors： Nishizawa M,Kamata M,Mojin T,Nakai Y,Aida Y

更新日期：2000-10-10 00:00:00

abstract：:Toscana (TOS) virus stocks strongly interfering with standard virus replication were obtained by sequential passages of virus in suckling mouse brain. Characterization of viral RNAs in these stocks showed the presence of a heterogeneous population of defective RNA molecules derived from the L genomic segment, in both ...

journal_title：Virology

authors： Marchi A,Nicoletti L,Accardi L,Di Bonito P,Giorgi C

更新日期：1998-06-20 00:00:00

abstract：:Soluble RNA-dependent RNA polymerases from healthy and alfalfa mosaic virus (AMV)-infected leaves were purified more than 400-fold from the 100,000g supernatant of leaf homogenates, using ammonium sulfate precipitation, Sephadex G-100 filtration, and chromatography on DEAE Sephadex A25 and P11-phosphocellulose. The DE...

journal_title：Virology

authors： Chifflot S,Sommer P,Hartmann D,Stussi-Garaud C,Hirth L

更新日期：1980-01-15 00:00:00

abstract：:Autoradiography of dodecyl sulfate-impregnated polyacrylamide gels after electrophoresis of the proteins from 32P-labeled cauliflower mosaic virus (CaMV) showed that two of the structural proteins were phosphorylated. These two proteins of 44 and 58 kilodaltons (kd) occur as minor components with some strains of the v...

journal_title：Virology

authors： Hahn P,Shepherd RJ

更新日期：1980-11-01 00:00:00

abstract：:Wild-type vaccinia virus WR strain forms non-fusogenic (F-) large plaques and is hemagglutinin positive (HA+) under normal conditions of virus infection. We have analyzed a collection of spontaneous, highly attenuated mutants of vaccinia virus isolated from persistently infected Friend erythroleukemia cells (E. Paez, ...

journal_title：Virology

authors： Ortiz MA,Paez E

更新日期：1994-01-01 00:00:00

abstract：:We describe studies of woodchuck hepatitis virus nucleic acids in liver and other tissues of chronically infected woodchucks, using Southern and Northern blot hybridization techniques. Single-stranded and covalently closed circular replicative DNA molecules were distinguished from partly double-stranded virus genomes....

journal_title：Virology

authors： Ogston CW,Schechter EM,Humes CA,Pranikoff MB

更新日期：1989-03-01 00:00:00

abstract：:Various lectins were tested for blocking rotavirus infection of MA104 cells and it was observed that galactose-specific lectins were the most inhibitory. Of these Ricinus agglutinin was able to inhibit infection (by human and animal strains) at concentrations as low as 10(-9) M. In addition, in a virus overlay protein...

journal_title：Virology

authors： Jolly CL,Beisner BM,Holmes IH

更新日期：2000-09-15 00:00:00

abstract：:Pea enation mosaic virus (PEMV) RNA was isolated from virions and then translated in rabbit reticulocyte lysates and in wheat germ extracts. RNA 1 (apparent molecular weight, Mr 1.77 x 106) was shown to code for two major translation products, vp2 (Mr 88,000) and vp4 (Mr 36,000) plus a high-molecular-weight minor prod...

journal_title：Virology

authors： Gabriel CJ,de Zoeten GA

更新日期：1984-12-01 00:00:00

abstract：:We determined the effect of the overproduction of viral nonstructural proteins on alphavirus plus-strand and minus-strand RNA synthesis. Because alphavirus minus-strand synthesis ceases normally at 3 to 4 hr postinfection and requires continuous protein synthesis [D. L. Sawicki and S. G. Sawicki, J. Virol. 34, 108-118...

journal_title：Virology

authors： Sawicki SG,Sawicki DL

更新日期：1986-07-30 00:00:00

abstract：:A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility. Comparison of the nucleotide sequences in the coat protein cistron of an aphid transmissible isolate (TVMV-AT) with that of T...

journal_title：Virology

authors： Atreya CD,Raccah B,Pirone TP

更新日期：1990-09-01 00:00:00