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MHV-A59 enters polarized murine epithelial cells through the apical surface but is released basolaterally.

Abstract：

:Coronaviruses have a marked tropism for epithelial cells. Entry and release of the porcine transmissible gastroenteritis virus (TGEV) is restricted to apical surfaces of polarized epithelial cells, as we have recently shown (J. W. A. Rossen, C. P. J. Bekker, W. F. Voorhout, G. J. A. M. Strous, A. van der Ende, and P. J. M. Rottier, 1994, J. Virol. 68, 7966-7973). In this paper we analyze the interactions of mouse hepatitis coronavirus A59 (MHV-A59) with polarized murine kidney cells (mTAL) grown on permeable supports. After inoculation from the apical or basolateral side, virus entry was found to take place only through the apical membrane. The virus utilized a protein of the carcinoembryonic antigen family as its receptor. In contrast to TGEV, MHV-A59 was released preferentially from the basolateral plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the basolateral culture fluid as well as by electron microscopical observations. In the mouse, MHV initially replicates in the nasal epithelium before being disseminated throughout the body; the basolateral release of MHV from epithelial cells into the animal's circulation may be the first step in the establishment of a systemic infection.

journal_name

Virology

journal_title

Virology

authors

Rossen JW,Voorhout WF,Horzinek MC,van der Ende A,Strous GJ,Rottier PJ

doi

10.1006/viro.1995.1316

subject

Has Abstract

pub_date

1995-06-20 00:00:00

pages

54-66

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(85)71316-5

journal_volume

210

pub_type

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