Protective role of Indoleamine 2,3 dioxygenase in Respiratory Syncytial Virus associated immune response in airway epithelial cells.

Abstract:

:RSV is a major cause of severe lower respiratory infection in infants and young children. With no vaccine yet available, it is important to clarify mechanisms of disease pathogenesis. Since indoleamine-2,3-dioxygenase (IDO) is an immunomodulatory enzyme and is upregulated with RSV infection, we studied it in vivo during infection of BALB/c mice and in vitro in A549 cells. RSV infection upregulated IDO transcripts in vivo and in vitro. IDO siRNA decreased IDO transcripts ~2 fold compared to control siRNA after RSV infection but this decrease did not affect RSV replication. In the presence of IFN-γ, siRNA-induced a decrease in IDO expression that was associated with an increase in virus replication and increased levels of IL-6, IL-8, CXCL10 and CCL4. Thus, our results show IDO is upregulated with RSV infection and this upregulation likely participates with IFN-γ in inhibition of virus replication and suppression of some host cell responses to infection.

journal_name

Virology

journal_title

Virology

authors

Rajan D,Chinnadurai R,O'Keefe EL,Boyoglu-Barnum S,Todd SO,Hartert TV,Galipeau J,Anderson LJ

doi

10.1016/j.virol.2017.09.007

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

144-150

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(17)30307-0

journal_volume

512

pub_type

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