Protective role of Indoleamine 2,3 dioxygenase in Respiratory Syncytial Virus associated immune response in airway epithelial cells.


:RSV is a major cause of severe lower respiratory infection in infants and young children. With no vaccine yet available, it is important to clarify mechanisms of disease pathogenesis. Since indoleamine-2,3-dioxygenase (IDO) is an immunomodulatory enzyme and is upregulated with RSV infection, we studied it in vivo during infection of BALB/c mice and in vitro in A549 cells. RSV infection upregulated IDO transcripts in vivo and in vitro. IDO siRNA decreased IDO transcripts ~2 fold compared to control siRNA after RSV infection but this decrease did not affect RSV replication. In the presence of IFN-γ, siRNA-induced a decrease in IDO expression that was associated with an increase in virus replication and increased levels of IL-6, IL-8, CXCL10 and CCL4. Thus, our results show IDO is upregulated with RSV infection and this upregulation likely participates with IFN-γ in inhibition of virus replication and suppression of some host cell responses to infection.






Rajan D,Chinnadurai R,O'Keefe EL,Boyoglu-Barnum S,Todd SO,Hartert TV,Galipeau J,Anderson LJ




Has Abstract


2017-12-01 00:00:00














  • Enhancement of dengue virus translation: role of the 3' untranslated region and the terminal 3' stem-loop domain.

    abstract::An essential step for a productive infection by the dengue flavivirus (DEN) is translation of the m(7)G-capped, nonpolyadenylated positive-sense RNA genome. We have recently identified sequences within the DEN 3' untranslated region (UTR) that modulate viral translation. Here, we show that the DEN type 2 (DEN2) 3'UTR ...


    pub_type: 杂志文章


    authors: Holden KL,Harris E

    更新日期:2004-11-10 00:00:00

  • Differential utilisation of ceramide during replication of the flaviviruses West Nile and dengue virus.

    abstract::It is well established that +ssRNA viruses manipulate cellular lipid homoeostasis and distribution to facilitate efficient replication. Here, we show that the cellular lipid ceramide is redistributed to the West Nile virus strain Kunjin virus (WNVKUN) replication complex (RC) but not to the dengue virus serotype 2 str...


    pub_type: 杂志文章


    authors: Aktepe TE,Pham H,Mackenzie JM

    更新日期:2015-10-01 00:00:00

  • The in vitro translation of Pea enation mosaic virus RNA.

    abstract::Pea enation mosaic virus (PEMV) RNA was isolated from virions and then translated in rabbit reticulocyte lysates and in wheat germ extracts. RNA 1 (apparent molecular weight, Mr 1.77 x 106) was shown to code for two major translation products, vp2 (Mr 88,000) and vp4 (Mr 36,000) plus a high-molecular-weight minor prod...


    pub_type: 杂志文章


    authors: Gabriel CJ,de Zoeten GA

    更新日期:1984-12-01 00:00:00

  • Cauliflower mosaic virus gene II product forms distinct inclusion bodies in infected plant cells.

    abstract::Turnip leaves infected with the aphid transmissible isolate of cauliflower mosaic virus (CaMV Cabb B-JI) showed two types of virus-containing inclusion bodies (IBs), which differed morphologically and in their protein composition when analyzed by immunogold labeling of ultrathin sections. Vacuolated IBs, typical of Ca...


    pub_type: 杂志文章


    authors: Espinoza AM,Medina V,Hull R,Markham PG

    更新日期:1991-11-01 00:00:00

  • A high throughput Cre-lox activated viral membrane fusion assay identifies pharmacological inhibitors of HIV entry.

    abstract::Enveloped virus entry occurs when viral and cellular membranes fuse releasing particle contents into the target cell. Human immunodeficiency virus (HIV) entry occurs by cell-free virus or virus transferred between infected and uninfected cells through structures called virological synapses. We developed a high-through...


    pub_type: 杂志文章


    authors: Esposito AM,Cheung P,Swartz TH,Li H,Tsibane T,Durham ND,Basler CF,Felsenfeld DP,Chen BK

    更新日期:2016-03-01 00:00:00

  • Plasticity of a critical antigenic determinant in the West Nile virus NY99 envelope protein domain III.

    abstract::West Nile virus (WNV) is a mosquito-borne flavivirus that causes febrile illness, encephalitis, and occasionally death in humans. The envelope protein is the main component of the WNV virion surface, and domain III of the envelope protein (EIII) is both a putative receptor binding domain and a target of highly specifi...


    pub_type: 杂志文章


    authors: Plante JA,Torres M,Huang CY,Beasley DWC

    更新日期:2016-09-01 00:00:00

  • Induction of type-specific neutralizing antibodies by capsomeres of human papillomavirus type 33.

    abstract::The immunogenicity of capsomeres of human papillomavirus type 33 was evaluated in a dose-response analysis. Capsomeres were obtained free of capsids by expression of L1 carrying the single point mutation C427S. Neutralizing antibodies were detected using an in vitro pseudoinfection assay. Capsomeres induced type-speci...


    pub_type: 杂志文章


    authors: Fligge C,Giroglou T,Streeck RE,Sapp M

    更新日期:2001-05-10 00:00:00

  • Temperature and density dependent induction of a cytopathic effect following infection with non-cytopathic HAV strains.

    abstract::Hepatitis A virus infection and growth in cultured cells is protracted, cell-type restricted, and generally not accompanied by the appearance of a cytopathic effect, with the exception of some culture-adapted strains. We demonstrate that the non-cytopathic HAV strain HM175/clone 1 can be induced to exhibit a cytopathi...


    pub_type: 杂志文章


    authors: Wales SQ,Ngo D,Hida K,Kulka M

    更新日期:2012-08-15 00:00:00

  • Protein-primed RNA synthesis in vitro by the virion-associated RNA polymerase of infectious pancreatic necrosis virus.

    abstract::The 94-kDa virion-associated RNA-dependent RNA polymerase (RdRp) is present in infectious pancreatic necrosis virus in two forms: (i) as a free polypeptide (VP1) and (ii) as a genome linked protein (VPg) (J. G. Calvert et al., 1991, J. Gen. Virol. 72, 2563-2567). VP1 was guanylylated in vitro by incubating purified vi...


    pub_type: 杂志文章


    authors: Dobos P

    更新日期:1995-04-01 00:00:00

  • The carboxy-terminal domain is essential for stability and not for virion incorporation of HIV-1 Vpr into virus particles.

    abstract::Vpr is one of the auxiliary gene products encoded by HIV-1 genome. Vpr is a 14-kDa protein and exhibits several interesting characteristics including incorporation into virus particles, oligomerization, localization in the nucleus, and positive regulation of virus replication in primary cells. In an effort to define t...


    pub_type: 杂志文章


    authors: Mahalingam S,Patel M,Collman RG,Srinivasan A

    更新日期:1995-12-20 00:00:00

  • Characterization of human papillomavirus type 6b DNA isolated from an invasive squamous carcinoma of the vulva.

    abstract::Human papillomavirus type 6 (HPV 6) DNA was detected in an episomal form in DNA extracted from an invasive squamous carcinoma of the vulva. The viral DNA, designated HPV6-T70, was molecularly cloned. Restriction analysis of the HPV6-T70 genome revealed an insertion of approximately 35 bp in the 5' portion of the upstr...


    pub_type: 杂志文章


    authors: Kasher MS,Roman A

    更新日期:1988-07-01 00:00:00

  • Replication of the murine cytomegalovirus genome: structure and role of the termini in the generation and cleavage of concatenates.

    abstract::Following infection with murine cytomegalovirus (MCMV), the termini of the linear double-stranded DNA genome fuse to form circular or concatemeric forms which serve as replicative intermediates. To investigate the mechanisms involved in the generation and cleavage of the intracellular concatenates, we have used restri...


    pub_type: 杂志文章


    authors: Marks JR,Spector DH

    更新日期:1988-01-01 00:00:00

  • Origin of the hemagglutinin gene of H3N2 influenza viruses from pigs in China.

    abstract::Influenza viruses of the H3N2 subtype similar to Aichi/2/68 and Victoria/3/75 persist in pigs many years after their antigenic counterparts have disappeared from humans (Shortridge et al. (1977). Science 19, 1454-1455). To provide information on the mechanism of conservation of these influenza viruses in pigs, the hem...


    pub_type: 杂志文章


    authors: Kida H,Shortridge KF,Webster RG

    更新日期:1988-01-01 00:00:00

  • Biologically active protease 3C of human rhinovirus 1A is expressed from a cloned cDNA segment in Escherichia coli.

    abstract::We produced the putative protease 3C of human rhinovirus 1A (HRV-1A), a minor group rhinovirus, by Escherichia coli expression of a segment of HRV-1A cDNA coding for 3A, 3B, 3C, and parts of 2C and 3D (delta 2C3ABC delta 3D). The protease 3C was expected to be processed by intramolecular Q-G cleavages from the virus-s...


    pub_type: 杂志文章


    authors: Aschauer B,Werner G,McCray J,Rosenwirth B,Bachmayer H

    更新日期:1991-10-01 00:00:00

  • Specific cell surface requirements for the infection of CD4-positive cells by human immunodeficiency virus types 1 and 2 and by Simian immunodeficiency virus.

    abstract::Human CD4 was expressed on a range of mammalian cell lines. CD4+ non-primate cells, derived from rat, hamster, mink, cat, and rabbit, bind recombinant gp120 of human immunodeficiency virus type 1 (HIV-1) but are resistant to HIV-1 infection. CD4 expression on various human, rhesus, and African green monkey cell lines ...


    pub_type: 杂志文章


    authors: Clapham PR,Blanc D,Weiss RA

    更新日期:1991-04-01 00:00:00

  • HIV-1 integration is inhibited by stimulation of the VPAC2 neuroendocrine receptor.

    abstract::Successful HIV-1 infection requires a number of specific stages leading to integration of the provirus. We previously suggested that members of the VPAC neuroendocrine receptor family may play a role in HIV-1 infection. We now show that stimulation of the VPAC2 receptor with specific agonists provides strong resistanc...


    pub_type: 杂志文章


    authors: Bokaei PB,Ma XZ,Sakac D,Branch DR

    更新日期:2007-05-25 00:00:00

  • Tyrosine sulfation of varicella-zoster virus envelope glycoprotein gpl.

    abstract::Sulfation is a common post-translational modification of secreted and membrane proteins, with the sulfate attached to tyrosine residues or to glycan side-chains. I have shown that varicella-zoster virus (VZV) envelope glycoproteins gpI, gpII, and gpIII can be labeled with [35S]sulfate. The predominant VZV glycoprotein...


    pub_type: 杂志文章


    authors: Edson CM

    更新日期:1993-11-01 00:00:00

  • A system for the analysis of BKV non-coding control regions: application to clinical isolates from an HIV/AIDS patient.

    abstract::The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BKV exists as archetype virus and rearranged variants, w...


    pub_type: 杂志文章


    authors: Broekema NM,Abend JR,Bennett SM,Butel JS,Vanchiere JA,Imperiale MJ

    更新日期:2010-11-25 00:00:00

  • Mutational analysis of the translational signal in the human cytomegalovirus gpUL4 (gp48) transcript leader by retroviral infection.

    abstract::A short upstream open reading frame (uORF2) in the human cytomegalovirus (CMV) gpUL4 (gp48) transcript leader is conserved among CMV strains and inhibits translation of a downstream cistron. Remarkably, this inhibitory effect depends on the amino acid coding information of uORF2, at least in transient transfection ass...


    pub_type: 杂志文章


    authors: Cao J,Geballe AP

    更新日期:1994-11-15 00:00:00

  • Members of the HCMV US12 family of predicted heptaspanning membrane proteins have unique intracellular distributions, including association with the cytoplasmic virion assembly complex.

    abstract::The human cytomegalovirus (HCMV) US12 gene family is a group of 10 predicted seven-transmembrane domain proteins that have some features in common with G-protein-coupled receptors. Little is known of their patterns of expression, localization, or functional interactions. Here, we studied the intracellular localization...


    pub_type: 杂志文章


    authors: Das S,Pellett PE

    更新日期:2007-05-10 00:00:00

  • Role of matrix protein in the type D retrovirus replication cycle: importance of the arginine residue at position 55.

    abstract::We previously reported that a mutant of Mason-Pfizer monkey virus (M-PMV), which has an amino acid substitution in the matrix (MA) protein at position 55, MA-R55W, showed altered viral morphogenesis, reduced glycoprotein incorporation, and loss of infectivity. In this report, we show that two additional amino acid sub...


    pub_type: 杂志文章


    authors: Yasuda J,Hunter E

    更新日期:2000-03-15 00:00:00

  • Adenovirus type 5 pseudotyped with adenovirus type 37 fiber uses sialic acid as a cellular receptor.

    abstract::For purposes of gene therapy, the tropism of adenovirus (Ad) serotype 5 vectors can be altered with fibers derived from alternative serotypes. However, there is currently limited information available on the cellular receptors used by the approximately 51 known Ad serotypes. Recently, alpha(2-->3)-linked sialic acid (...


    pub_type: 杂志文章


    authors: Cashman SM,Morris DJ,Kumar-Singh R

    更新日期:2004-06-20 00:00:00

  • Reovirus exists in the form of 13 particle species that differ in their content of protein sigma 1.

    abstract::When electrophoresed in 0.7% agarose gels, populations of reovirus particles can be resolved into 13 well-defined bands that possess from 0 to 12 projection/spike-associated trimers of protein sigma 1. This state of affairs is not an artifact of purification, of the techniques used to demonstrate it, of aggregation, o...


    pub_type: 杂志文章


    authors: Larson SM,Antczak JB,Joklik WK

    更新日期:1994-06-01 00:00:00

  • Human papillomavirus types 16 E6 and E7 contribute differently to carcinogenesis.

    abstract::High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...


    pub_type: 杂志文章


    authors: Song S,Liem A,Miller JA,Lambert PF

    更新日期:2000-02-15 00:00:00

  • Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector.

    abstract::We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formati...


    pub_type: 杂志文章


    authors: LaFace D,Hermonat P,Wakeland E,Peck A

    更新日期:1988-02-01 00:00:00

  • Recombinant measles viruses defective for RNA editing and V protein synthesis are viable in cultured cells.

    abstract::The measles virus (MV) phosphoprotein (P) gene encodes three proteins, P, C, and V. The V protein is synthesized by pseudo-templated transcription, also designated as RNA editing: during P gene transcription one G residue is inserted at a defined position in about 50% of the mRNAs. To study the importance of sequence ...


    pub_type: 杂志文章


    authors: Schneider H,Kaelin K,Billeter MA

    更新日期:1997-01-20 00:00:00

  • Cell surface binding simian virus 40 large T antigen becomes anchored and stably linked to lipid of the target cells.

    abstract::Previously a new small subclass of SV40 large T antigen with a high-binding affinity to living target cells was characterized (J. Lange-Mutschler and R. Henning, 1983, Virology 127, 333-344.) In the present study the external binding process, particularly the tight linkage of T antigen to lipid of the target cells, wa...


    pub_type: 杂志文章


    authors: Lange-Mutschler J,Henning R

    更新日期:1984-07-30 00:00:00

  • Structural variation of La Crosse virions under different chemical and physical conditions.

    abstract::La Crosse (LAC) virions exposed to different pHs (7.3, 6.2, and 5.4) and temperatures (37 degrees, 20 degrees, and 4 degrees) were preserved in thin layers of vitreous ice and observed by electron cryomicroscopy. Our results indicate that, at lower pH, virus particles interact with each other to form aggregates. In so...


    pub_type: 杂志文章


    authors: Wang GJ,Hewlett M,Chiu W

    更新日期:1991-09-01 00:00:00

  • Evidence that RNA 4 of alfalfa mosaic virus does not replicate autonomously.

    abstract::A mutant (Tbts7) of alfalfa mosaic virus, the coat protein of which is unable to activate the viral genome (the RNA species 1, 2, and 3, which need some coat protein for infectivity) at 30 degrees , can be rescued at this temperature by adding to the inoculum wild-type RNA 3 (the genome part that contains the coat pro...


    pub_type: 杂志文章


    authors: Smit CH,Jaspars EM

    更新日期:1982-02-01 00:00:00

  • Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell-cell fusion.

    abstract::The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell...


    pub_type: 杂志文章


    authors: Schowalter RM,Wurth MA,Aguilar HC,Lee B,Moncman CL,McCann RO,Dutch RE

    更新日期:2006-07-05 00:00:00