Abstract:
:The interferon (IFN) activity found in human leukocyte IFN alpha preparations, autoimmune and AIDS sera, and others was reported to have distinct antigenic and deviating biological properties. This led to its vague designation as acid-labile and thermolabile IFN alpha. However, using specific monoclonal antibodies, the acid-labile component of IFN alpha (not exposed to pH 2) and recombinant IFN omega 1 showed significant relatedness. Monoclonal antibody T19, generated with virus-induced leukocyte IFN alpha that had not been exposed to pH 2, neutralized both the antiviral and antiproliferative activities of IFN omega-1, and vice versa; monoclonal antibody OMG 5, specific for recombinant IFN omega-1, cross-neutralized the antiviral and antiproliferative effects of the acid-labile component of leukocyte IFN alpha. When these two IFN preparations were incubated at pH 2 for 72 hr, their biological activity significantly decreased.
journal_name
Virologyjournal_title
Virologyauthors
Kontsek P,Borecký L,Novák Mdoi
10.1016/0042-6822(91)90517-fsubject
Has Abstractpub_date
1991-03-01 00:00:00pages
416-8issue
1eissn
0042-6822issn
1096-0341journal_volume
181pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::La Crosse (LAC) virions exposed to different pHs (7.3, 6.2, and 5.4) and temperatures (37 degrees, 20 degrees, and 4 degrees) were preserved in thin layers of vitreous ice and observed by electron cryomicroscopy. Our results indicate that, at lower pH, virus particles interact with each other to form aggregates. In so...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90869-d
更新日期:1991-09-01 00:00:00
abstract::We used a mouse nasal model of herpes simplex virus 2 (HSV-2) infection to examine the biological properties of HSV-2 wild-type (wt), TK-negative, and replication-defective strains in vivo. Nasal septa tissue is the major site of wt viral replication post intranasal (i.n.) inoculation. The HSV-2 strain 186 syn(+)-1 wt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0628
更新日期:2000-12-05 00:00:00
abstract::Chlorella virus PBCV-1 encodes two putative chitinase genes, a181/182r and a260r, and one chitosanase gene, a292l. The three genes were cloned and expressed in Escherichia coli. The recombinant A181/182R protein has endochitinase activity, recombinant A260R has both endochitinase and exochitinase activities, and recom...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9958
更新日期:1999-10-25 00:00:00
abstract::Bacteriophage phi 29 DNA polymerase is sensitive to aphidicolin (APH). DNA polymerase of the APH-resistant mutant, APHr71, was more sensitive to phosphonoacetic acid and butylphenyldeoxyguanosine 5'triphosphate than the wild type. Nucleotide sequence analysis revealed a single transition of G at nucleotide 562 to A in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90416-o
更新日期:1990-09-01 00:00:00
abstract::Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.02.016
更新日期:2008-05-10 00:00:00
abstract::In a previous study, we observed that hepatitis C virus (HCV) core protein specifically inhibits translation initiated by an HCV internal ribosome entry site (IRES). To investigate the mechanism by which down-regulation of HCV translation occurs, a series of mutations were introduced into the IRES element, as well as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.013
更新日期:2006-02-20 00:00:00
abstract::Coat protein-protected fragments of alfalfa mosaic virus RNA (AlMV-RNA) and tobacco streak virus RNA (TSV-RNA), which were isolated as described [D. Zuidema, M. F. A. Bierhuizen, B. J. C. Cornelissen, J. F. Bol, and E. M. J. Jaspars (1983)Virology, 125, 361-369], were tested for their ability to rebind AlMV coat prote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90165-x
更新日期:1984-07-30 00:00:00
abstract::A previous study suggested that the genomes of the arenaviruses native to North America are a product of genetic recombination between New World arenaviruses with significantly different phylogenetic histories. The purpose of this study was to extend our knowledge of the principal host relationships and evolutionary h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.05.031
更新日期:2007-10-25 00:00:00
abstract::Retroviruses are expressed under the control of viral control regions designated long terminal repeats (LTRs), which contain all signals for transcriptional initiation as well as transcriptional termination. However, retroviral LTRs from different species within a common genus, such as Lentivirus, do not show signific...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0527
更新日期:1996-10-01 00:00:00
abstract::Genetic variants surrounding the interferon-λ3 (IFNL3) gene are strongly associated with clearance of hepatitis C virus (HCV). A variant (rs368234815 TT/ΔG) upstream of IFNL3 was recently implicated to control expression of a novel gene termed IFNL4. We conducted genetic analysis of rs368234815 in a chronic HCV patien...
journal_title:Virology
pub_type: 临床试验,杂志文章
doi:10.1016/j.virol.2014.12.020
更新日期:2015-02-01 00:00:00
abstract::The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of int...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90494-c
更新日期:1990-07-01 00:00:00
abstract::Feline panleukopenia virus (FPV) is a pathogen whose canine-adapted form (canine parvovirus (CPV)) emerged in 1978. These viruses infect by binding host transferrin receptor type-1 (TfR), but also hemagglutinate erythrocytes. We show that hemagglutination involves selective recognition of the non-human sialic acid N-g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.02.009
更新日期:2013-05-25 00:00:00
abstract::Infectious bronchitis virus (IBV) infection and associated illness may be dramatically modified by passive transfer of immune T lymphocytes. Lymphocytes collected 10 days postinfection were transferred to naive chicks before challenge with virus. As determined by respiratory illness and viral load, transfer of syngene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0211
更新日期:2000-03-30 00:00:00
abstract::We have investigated the effect of a monoclonal antibody on influenza virus release and the cell surface expression of M2, comparing virus strains which were observed previously to be sensitive (A/Udorn) or resistant (A/WSN and A/Udorn variants) to growth inhibition by M2 antibody 14C2. Incubation of A/Udorn virus-inf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1498
更新日期:1995-10-01 00:00:00
abstract::While several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed to be monocytes which are poorly infected directly, but are highly susce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.07.010
更新日期:2011-09-15 00:00:00
abstract::Middle East respiratory syndrome coronavirus (MERS-CoV) infects host cells through binding the receptor binding domain (RBD) on its spike glycoprotein to human receptor dipeptidyl peptidase 4 (hDPP4). Here, we report identification of critical residues on hDPP4 for RBD binding and virus entry through analysis of a pan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.10.006
更新日期:2014-12-01 00:00:00
abstract::West Nile virus (WNV) genome cyclization is mediated by two pairs of long-distance RNA/RNA interactions: the 5'CS/3'CSI (conserved sequence) and the 5'UAR/3'UAR (upstream AUG region) base pairings. Antisense peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs), designed to interfere with the 5'CS/3'CSI o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.044
更新日期:2008-12-05 00:00:00
abstract::African Swine fever virus (ASFV) genome is a large (170-190 kb) double-stranded DNA molecule with structural features similar to those of Poxviruses. Prominent among those features are the presence of a hairpin loop structure at the end of the DNA molecule and terminal-inverted repeats (TIR). The TIRs have been previo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1277
更新日期:1994-05-15 00:00:00
abstract::Of five globally important VP7 (G) serotypes (G1-4 and 9) of group A rotaviruses (the single most important etiologic agents of infantile diarrhea worldwide), G9 continues to attract considerable attention because of its unique natural history. Serotype G9 rotavirus was isolated from a child with diarrhea first in the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.11.006
更新日期:2005-02-05 00:00:00
abstract::Primary varicella-zoster virus (VZV) infection of humans may result in latent infection of sensory neurons in the peripheral nervous system. To examine the interaction of VZV with the sensory neuron we infected immunochemically defined human neurons with cell-associated VZV. Utilizing double-label immunofluorescence t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90141-8
更新日期:1986-07-30 00:00:00
abstract::Cytoplasmic tails of envelope (Env) glycoproteins of many retroviruses inhibit their membrane fusion activity. The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.04.005
更新日期:2019-06-01 00:00:00
abstract::Primary strains of human immunodeficiency virus type 1 (HIV-1) are known to adapt to replication in cell lines in vitro by becoming sensitive to soluble CD4 (sCD4) and neutralizing antibodies (NAb). T-cell lines favor isolation of variants that use CXCR4 as a co-receptor, while primary isolates predominantly use CCR5....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.012
更新日期:2004-03-30 00:00:00
abstract::In this study, we show how the red spotted grouper nervous necrosis virus (RGNNV) causes loss of mitochondrial membrane potential and promotes host secondary apoptotic necrosis. RGNNV viral proteins such as protein alpha (42 kDa) and protein A (110 kDa) were quickly expressed between 12 h and 24 h postinfection (p.i.)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.052
更新日期:2006-04-10 00:00:00
abstract::The envelope glycoprotein (Env) of Moloney murine leukemia virus (MoMuLV) is proteolytically processed and transported to the cell surface where it can be incorporated into budding virions. Cell surface Env is frequently detected using an indirect immunofluorescence assay and fluorescence-activated cell sorting (FACS)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9073
更新日期:1998-04-10 00:00:00
abstract::We test the hypothesis that the translation machinery in cells infected by influenza A virus efficiently translates only mRNAs that possess the influenza viral 5' untranslated region (5'-UTR) by introducing mRNAs directly into the cytoplasm of infected cells. This strategy avoids effects due to the inhibition of the n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1149
更新日期:2001-10-25 00:00:00
abstract::Measles virus (MV) causes various responses including the induction of immune responses, transient immunosuppression and establishment of long-lasting immunity. To obtain a comprehensive view of the effects of MV infection on target cells, DNA microarray analyses of two different cell-types were performed. An epitheli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.02.015
更新日期:2008-06-05 00:00:00
abstract::The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography em...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90331-x
更新日期:1987-03-01 00:00:00
abstract::Callitrichid hepatitis (CH) is a highly fatal, rodent-borne zoonosis of New World primates (family Callitrichidae) caused by lymphocytic choriomeningitis virus (LCMV). It is unclear whether virulence in Callitrichidae is associated with specific genetic or phylogenetic markers of the virus as only a partial S RNA sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0909
更新日期:2001-06-05 00:00:00
abstract::The helix-destabilizing properties of a single-stranded DNA-binding protein, LEF-3, of Bombyx mori nucleopolyhedrovirus (BmNPV) were studied. Partial duplexes of DNA containing single-stranded (ss) tails of different sizes and orientations were used as substrates for assay of the unwinding ability of LEF-3. Upon nonco...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0270
更新日期:2000-04-25 00:00:00
abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0002
更新日期:1996-01-01 00:00:00