Abstract:
:The pregenomic RNA (pgRNA) of hepadnaviruses serves a dual role: as mRNA for the core (C) and polymerase (P) synthesis and as an RNA template for viral genome replication. A question arises as to how these two roles are regulated. We hypothesized that the P protein could suppress translation of the pgRNA via its interaction with 5' stem-loop structure (epsilon or encapsidation signal). Consistent with the hypothesis, we observed up-regulation of the C protein level in the absence of the P protein expression in a physiological context. Importantly, translational suppression depended on the 5' epsilon sequence. Furthermore, the impact of the P protein on ongoing translation of the C ORF was directly demonstrated by polysome distribution analysis. We conclude that the P protein suppresses translation of the pgRNA via a mechanism involving its interaction with the 5' epsilon sequence, a finding that implicates the coordinated switch from translation to genome replication.
journal_name
Virologyjournal_title
Virologyauthors
Ryu DK,Kim S,Ryu WSdoi
10.1016/j.virol.2007.11.010subject
Has Abstractpub_date
2008-03-30 00:00:00pages
112-23issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(07)00758-1journal_volume
373pub_type
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