Abstract:
:The role of the matrix (MA) domain of simian immunodeficiency virus (SIV) and bovine leukaemia virus (BLV) Gag in the assembly of virus-like particles (VLP) in insect cells has been investigated. Wild-type SIV and BLV Gag assembled to form discrete VLP structures typical of many retroviruses analysed by similar systems. When amino acids predicated by the three-dimensional structure to be at the interface of SIV MA monomers were deleted, VLP assembly was abolished consistent with a role for MA multimerization in assembly. When amino acids predicted to be in the analogous positions in BLV MA were mutated, however, VLP assembly was not affected. These data indicate that the models of assembly derived from one model retrovirus may not necessarily apply to more distantly related viruses despite the structural similarity present in equivalent Gag domains.
journal_name
Virologyjournal_title
Virologyauthors
Kakker NK,Mikhailov MV,Jones IM,Roy Pdoi
10.1006/viro.2002.1441subject
Has Abstractpub_date
2002-07-20 00:00:00pages
48-55issue
1eissn
0042-6822issn
1096-0341pii
S0042682202914418journal_volume
299pub_type
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