Abstract:
:Coronavirus (CoV) envelope (E) protein ion channel activity was determined in channels formed in planar lipid bilayers by peptides representing either the transmembrane domain of severe acute respiratory syndrome CoV (SARS-CoV) E protein, or the full-length E protein. Both of them formed a voltage independent ion conductive pore with symmetric ion transport properties. Mutations N15A and V25F located in the transmembrane domain prevented the ion conductivity. E protein derived channels showed no cation preference in non-charged lipid membranes, whereas they behaved as pores with mild cation selectivity in negatively-charged lipid membranes. The ion conductance was also controlled by the lipid composition of the membrane. Lipid charge also regulated the selectivity of a HCoV-229E E protein derived peptide. These results suggested that the lipids are functionally involved in E protein ion channel activity, forming a protein-lipid pore, a novel concept for CoV E protein ion channel entity.
journal_name
Virologyjournal_title
Virologyauthors
Verdiá-Báguena C,Nieto-Torres JL,Alcaraz A,DeDiego ML,Torres J,Aguilella VM,Enjuanes Ldoi
10.1016/j.virol.2012.07.005subject
Has Abstractpub_date
2012-10-25 00:00:00pages
485-94issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(12)00335-2journal_volume
432pub_type
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