Abstract:
:Cell-mediated immunity plays a key role in the regression of papillomavirus-induced warts and intra-epithelial lesions but the target antigens that induce this response are not clear. Canine oral papillomavirus (COPV) infection of the oral cavity in dogs is a well-characterized model of mucosal papillomavirus infection that permits analysis of the immune events during the infectious cycle. In this study we show that during the COPV infectious cycle, systemic T cell responses to peptides of several early proteins particularly the E2 protein, as assayed by delayed type hypersensitivity, lymphoproliferation and IFN-gamma ELISPOT, can be detected. The maximal response occurs in a narrow time window that coincides with maximal viral DNA replication and wart regression: thereafter, systemic T cell responses to early proteins decline quite rapidly. Vaccination using particle-mediated immunotherapeutic delivery (PMID) of codon-modified COPV E2 and E1 genes induces strong antigen-specific cell-mediated immune responses in the vaccinated animals. These data show that therapeutic immunization by PMID with codon-modified E2 is completely effective, that to E1 is partially protective, that this correlates with the intensity of antigen-specific cell-mediated immune responses and, further, they emphasize the importance of these responses and the route of immunization in the generation of protective immunity.
journal_name
Virologyjournal_title
Virologyauthors
Jain S,Moore RA,Anderson DM,Gough GW,Stanley MAdoi
10.1016/j.virol.2006.07.032subject
Has Abstractpub_date
2006-12-05 00:00:00pages
23-34issue
1-2eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00499-5journal_volume
356pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Animal viruses profoundly modify the metabolism of lipids, the synthesis of new membranes, and membrane traffic. These alterations are related to the replication of viral genomes. Addition of oleic acid from the beginning of poliovirus infection inhibits the appearance of virus polypeptides at concentrations that do n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90802-i
更新日期:1991-11-01 00:00:00
abstract::A series of clones has been derived from an interferon-resistant murine cell line, Ltk- aprt-, and their antiviral properties have been characterized. In the parental Ltk- aprt- line interferon is unable to establish antiviral properties or to increase the levels of 2,5-oligo(A) synthetase, the 2,5-oligo(A)-activated ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90400-x
更新日期:1988-01-01 00:00:00
abstract::The Sindbis virus mutant SB-RL, in contrast to its parent, Sindbis strain AR339 (SB), is attenuated in neonatal mice, has an increased rate of penetration in tissue culture cells, and is more sensitive to neutralization by E2-specific monoclonal antibodies (MCAbs) R6 and R13. These phenotypic differences are controlle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90184-s
更新日期:1990-03-01 00:00:00
abstract::The virus SIRV1 of the extremely thermophilic archaeon Sulfolobus has a double-stranded DNA genome similar in architecture to the genomes of eukaryal viruses of the families Poxviridae, Pycodnaviridae, and Asfarviridae: the two strands of the 32,301 bp long linear genome are covalently connected forming a continuous p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0776
更新日期:2001-03-01 00:00:00
abstract::Cytoplasmic poly(A)-containing RNA from parainfluenza virus 3-infected cells was used as template to construct a cDNA library that was cloned into the EcoRV site of the plasmid pMG5. The resulting clones were screened with [32P]-labeled cDNA probes made from infected and mock-infected cell mRNAs. The virus specificity...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90237-5
更新日期:1985-11-01 00:00:00
abstract::Hypervariable region 1 (HVR1) proteins of hepatitis C virus (HCV) have been reported to react broadly with sera of patients with HCV infection. However, the variability of the broad reactivity of individual HVR1 proteins has not been elucidated. We assessed the reactivity of 25 different HVR1 proteins (genotype 1b) wi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0004
更新日期:1999-11-10 00:00:00
abstract::A major neutralizing epitope (here referred to as the T332 epitope) located on the lateral surface of domain III (DIII) of the West Nile virus (WNV) envelope protein has been identified based on the analysis of murine monoclonal antibodies. However, little is known about the humoral immune response against WNV in a na...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.047
更新日期:2007-03-15 00:00:00
abstract::Genetic variants surrounding the interferon-λ3 (IFNL3) gene are strongly associated with clearance of hepatitis C virus (HCV). A variant (rs368234815 TT/ΔG) upstream of IFNL3 was recently implicated to control expression of a novel gene termed IFNL4. We conducted genetic analysis of rs368234815 in a chronic HCV patien...
journal_title:Virology
pub_type: 临床试验,杂志文章
doi:10.1016/j.virol.2014.12.020
更新日期:2015-02-01 00:00:00
abstract::We report the complete nucleotide sequence of the bovine herpesvirus 1 (BHV-1) immediate-early gene encoding BICP4, the homolog of the ICP4 protein of herpes simplex virus. Combined with previous mapping studies, the sequence analysis revealed that the transcript for BICP4 consisted of a noncoding leader RNA (exon 1; ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1596
更新日期:1993-11-01 00:00:00
abstract::The primary objective of characterizing bovine adenovirus type 3 (BAV3) in greater detail is to develop it as a vector for gene therapy and vaccination of humans and animals. A series of BAV3 early region 4 (E4) deletion-mutant viruses, containing deletions in individual E4 open reading frames (Orf) or combinations of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1176
更新日期:2001-11-10 00:00:00
abstract::Transcription of the paramyxovirus RNA genome is thought to involve a sequential stop-start mechanism whereby monocistronic mRNAs are produced by polyadenylation and termination of 3' upstream gene followed by reinitiation at the downstream start site. For a number of paramyxoviruses, transcription across the M-F gene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9266
更新日期:1998-08-01 00:00:00
abstract::Female BALB/c mice were infected with coxsackievirus B3 in the diestrus, proestrus, estrus, or metestrus phases of the ovarian cycle. Cycle stage was determined by vaginal smear. All mice were killed 7 days after infection. Females infected in the diestrus and especially the proestrus phases developed myocarditis. CD4...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.06.051
更新日期:2004-12-05 00:00:00
abstract::The ability of HIV to match levels of viral mRNA to the activation state of the host cell may play a role in its ability to persist as well as to replicate. This linkage depends on the function of the viral transcriptional regulatory protein, Tat, which increases the efficiency of RNA elongation (transcriptional proce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9647
更新日期:1999-05-10 00:00:00
abstract::HIV-1 entry into cells requires the interaction of both HIV-1 envelope proteins and membrane lipids. We investigated the mechanism of neutralization of HIV-1 infection of primary monocyte-derived macrophages (MDM) by a murine monoclonal antibody (mAb) WR321. WR321 specifically binds phosphatidylinositol-4-phosphate an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.04.017
更新日期:2012-09-01 00:00:00
abstract::B-HIV1, an oligoclone of immortalized cells derived from human peripheral B lymphocytes infected in vitro with the TIIIB isolate of HIV-1, produces low levels of replication-competent HIV when propagated in 1% serum, but increases production > or = 5-fold after phorbol myristate acetate (PMA) exposure. Electron micros...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1499
更新日期:1993-10-01 00:00:00
abstract::The vaccinia virus temperature-sensitive mutations Cts6 and Cts9 were mapped by marker rescue and DNA sequencing to the A28 gene. Cts6 and Cts9 contain an identical 2-bp deletion truncating the A28 protein and removing the fourth conserved cysteine near the C-terminus. Cts9 mutant virions produced at 40 degrees C were...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.060
更新日期:2007-09-15 00:00:00
abstract::A novel virus isolated from the feather follicles of cockatoos diagnosed as having psittacine beak and feather disease was characterized by electron microscopy, nucleic acid content, and polypeptide composition. Purified virions displayed an icosahedral symmetry, were nonenveloped, and had a mean diameter of 14 to 16 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90513-8
更新日期:1989-07-01 00:00:00
abstract::Covalently closed circular dimers of phage lambda DNA have been found in Escherichia coli infected with lambda. These dimers can be formed by either the lambda Red or Int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda O and P genes or by joining of the cohesive ends. Dimers...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90136-2
更新日期:1977-09-01 00:00:00
abstract::The relationship between the cell cycle and the presence of the nuclear T antigen in simian virus 40-transformed rat cells independently derived from FR 3T3 cells has been investigated using a fluorescence-activated cell sorter and a combination of stainings for cellular DNA (4',6-diamidine-2-phenylindole dihydrochlor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(83)80028-2
更新日期:1983-04-30 00:00:00
abstract::Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in cont...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.12.018
更新日期:2016-03-01 00:00:00
abstract::A non-optimal receptor-binding specificity of avian influenza viruses is believed to hamper their replication in humans; however, the magnitude of this restriction remains undefined. Here we generated recombinant viruses, R1 and R2, that differed solely by two amino acids in the receptor-binding site of their hemagglu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.11.030
更新日期:2007-05-10 00:00:00
abstract::Brain metastases occur commonly in the setting of a variety of human cancers. At present, such cases are invariably fatal and highlight a need for research on new therapies. We have developed a mouse brain tumor model utilizing the Harding-Passey melanoma cell line injected intracranially into C57Bl/6 mice. Tumors dev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1382
更新日期:1995-08-01 00:00:00
abstract::Replication-deficient adenovirus (Ad) vectors provide an efficient technology for direct DNA delivery to cells both in vitro and in vivo. We have inserted the measles virus nucleoprotein (N) gene under the control of the strong constitutive CMV major IE promoter into an Ad type 5 E1- vector to produce the recombinant ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1362
更新日期:1995-07-10 00:00:00
abstract::Friend murine leukemia virus (Fr-MLV) produces unspliced mRNAs, as well as singly-spliced mRNAs by which the Env protein is generated. We investigated the role of the intron within the Fr-MLV gene in Env expression using vectors with serially truncated introns. The up-regulatory regions, the 361-878 and the 5135-5399 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.040
更新日期:2009-03-01 00:00:00
abstract::In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian clade 2.3.4.4 H5 highly pathogenic avian influenza (HPAI) virus, initially affecting mainly wild birds and few backyard and commercial poultry premises. To better model the outbreak, the pathogenesis and transmission dynamics of representative Eu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.019
更新日期:2016-07-01 00:00:00
abstract::The complete nucleotide sequence of cloned cDNAs corresponding to the full length of the mRNA encoding the measles virus hemagglutinin (H) protein has been determined. the mRNA contains a single large open reading frame which is capable of encoding a protein of 617 amino acids with a molecular mass of 69,250 Da. The d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90312-0
更新日期:1986-04-30 00:00:00
abstract::We have analyzed the limiting factors involved in the induction of human immunodeficiency virus type 1 (HIV-1) provirus expression by tumor necrosis factor-alpha (TNF-alpha), phorbol-12-myristate-13-acetate (PMA), and bryostatin-1 in T-cells (ACH-2) and monocytes (U1). We have demonstrated that, while there is a corre...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90295-z
更新日期:1992-03-01 00:00:00
abstract::Equine infectious anemia virus (EIAV) is responsible of acute disease episodes characterized by fever, anemia, thrombocytopenia and anorexia in equids. The high mutation rate in EIAV genome limited the number of full genome sequences availability. In the present study, we used the SureSelect target enrichment system w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.08.016
更新日期:2019-11-01 00:00:00
abstract::The capsid protein sequences of 10 representative parvoviruses were aligned against the sequence and three-dimensional structure of canine parvovirus (CPV). The structure of CPV was then analyzed after mapping onto it position-dependent sequence similarity scores and the locations of residues that are phenotypically i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1288
更新日期:1993-06-01 00:00:00
abstract::We used a series of deletion mutations in the 5' untranslated region of the prototype D type retrovirus, Mason-Pfizer Monkey Virus (MPMV), to analyse RNA encapsidation. A region was identified upstream of the major splice donor which reduced particle production but had a proportionally greater effect on RNA packaging....
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1061
更新日期:2001-09-15 00:00:00