Cauliflower mosaic virus gene II product forms distinct inclusion bodies in infected plant cells.


:Turnip leaves infected with the aphid transmissible isolate of cauliflower mosaic virus (CaMV Cabb B-JI) showed two types of virus-containing inclusion bodies (IBs), which differed morphologically and in their protein composition when analyzed by immunogold labeling of ultrathin sections. Vacuolated IBs, typical of CaMV infections, contained P62 (the generally accepted IB protein) but lacked P18 (the aphid transmission factor), while electron-lucent IBs did not contain P62 but were the only detectable sites of P18 accumulation within the infected leaf cells. Both types of inclusions were detected in cells of the epidermis, vascular bundles, mesophyll, and spongy parenchyma. Electron-lucent IBs were not found in the aphid nontransmissible isolates of CaMV, Campbell and CM4-184.






Espinoza AM,Medina V,Hull R,Markham PG




Has Abstract


1991-11-01 00:00:00
















  • Induction of circular episomes during rescue and replication of adeno-associated virus in experimental models of virus latency.

    abstract::The synthesis of linear duplex replicative structures (monomers, head-to-head, and tail-to-tail dimers) is an important hallmark of the productive phase of the adeno-associated virus (AAV) life cycle. These structures are generated by a strand-displacement replication mechanism and believed to be a reservoir for singl...


    pub_type: 杂志文章


    authors: Musatov SA,Scully TA,Dudus L,Fisher KJ

    更新日期:2000-09-30 00:00:00

  • Presentation of the HPV16E7 protein by skin grafts is insufficient to allow graft rejection in an E7-primed animal.

    abstract::The E7 transforming protein of Human Papillomavirus type 16 (HPV16) is expressed in the skin of a line of FVB mice transgenic for the E6 and E7 open reading frames of HPV16 driven from the alpha A crystallin promoter (FVB alpha AcryHPV16E6E7). We have transferred skin from FVB alpha AcryHPV16E6E7 mice to naive or E7-p...


    pub_type: 杂志文章


    authors: Dunn LA,Evander M,Tindle RW,Bulloch AL,de Kluyver RL,Fernando GJ,Lambert PF,Frazer IH

    更新日期:1997-08-18 00:00:00

  • Promoter, spliced leader, and coding sequence for BICP4, the largest of the immediate-early proteins of bovine herpesvirus 1.

    abstract::We report the complete nucleotide sequence of the bovine herpesvirus 1 (BHV-1) immediate-early gene encoding BICP4, the homolog of the ICP4 protein of herpes simplex virus. Combined with previous mapping studies, the sequence analysis revealed that the transcript for BICP4 consisted of a noncoding leader RNA (exon 1; ...


    pub_type: 杂志文章


    authors: Schwyzer M,Vlcek C,Menekse O,Fraefel C,Paces V

    更新日期:1993-11-01 00:00:00

  • Temporal and spatial expression of the E5a protein during the differentiation-dependent life cycle of human papillomavirus type 31b.

    abstract::Human papillomaviruses (HPVs) are epitheliotropic viruses, and their life cycle is intimately linked to the stratification and differentiation state of the host epithelial tissues. Defining a role for the E5 gene product in the differentiation-dependent viral life cycle has been difficult due to the lack of a suitable...


    pub_type: 杂志文章


    authors: Mayer TJ,Meyers C

    更新日期:1998-09-01 00:00:00

  • Correlation between polymerase activity and pathogenicity in two duck H5N1 influenza viruses suggests that the polymerase contributes to pathogenicity.

    abstract::The influenza RNA polymerase is known to be important in pathogenicity and adaptation of avian influenza viruses to mammalian hosts. However, the molecular mechanisms responsible are only partly understood. Here we investigated the role of the polymerase in two different, closely related, H5N1 influenza viruses - a hi...


    pub_type: 杂志文章


    authors: Leung BW,Chen H,Brownlee GG

    更新日期:2010-05-25 00:00:00

  • Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia.

    abstract::Rotaviral RNA was detected in the stool sample of an asymptomatic fattening pig at a Slovenian pig farm. To characterize the rotavirus, RT-PCR was used, employing primers specific for the VP7, VP4 and NSP4 genes. Specific products were purified and the sequencing reaction was performed for the molecular analysis of am...


    pub_type: 杂志文章


    authors: Steyer A,Poljsak-Prijatelj M,Barlic-Maganja D,Jamnikar U,Mijovski JZ,Marin J

    更新日期:2007-03-15 00:00:00

  • Bombyx mori nucleopolyhedrovirus BM5 protein regulates progeny virus production and viral gene expression.

    abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mo...


    pub_type: 杂志文章


    authors: Kokusho R,Koh Y,Fujimoto M,Shimada T,Katsuma S

    更新日期:2016-11-01 00:00:00

  • NS3 protein of hepatitis C virus regulates cyclooxygenase-2 expression through multiple signaling pathways.

    abstract::Hepatitis C virus (HCV) causes chronic hepatitis, which often results in the development of liver cirrhosis and hepatocellular carcinoma (HCC) worldwide. In this study, we demonstrated that the non-structural protein NS3 of HCV enhances cyclooxygenase-2 (COX-2) gene promoter activity, COX-2 mRNA expression, COX-2 prot...


    pub_type: 杂志文章


    authors: Lu L,Wei L,Peng G,Mu Y,Wu K,Kang L,Yan X,Zhu Y,Wu J

    更新日期:2008-02-05 00:00:00

  • The distribution and kinetics of polyomavirus in lungs of intranasally infected newborn mice.

    abstract::The primary cell types that sustain polyomavirus (Py) replication following intranasal infection as well as the nature of the host cellular response to Py were unknown. As this is an essential and specific site for virus entry, it seems likely that viral gene function must be adapted to these mucosal tissues. Using im...


    pub_type: 杂志文章


    authors: Gottlieb K,Villarreal LP

    更新日期:2000-01-05 00:00:00

  • Early activation of mitogen-activated protein kinase kinase, extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and c-Jun N-terminal kinase in response to binding of simian immunodeficiency virus to Jurkat T cells expressing CCR5

    abstract::We have shown that the binding of simian immunodeficiency virus (SIV) to Jurkat T cells expressing CD4 receptor strongly induces mitogen-activated protein (MAP) kinase kinase (MEK) and extracellular signal-regulated kinases 1 and 2 (ERK1/2) and only weakly induces p38 MAP kinase and c-Jun N-terminal kinase (JNK). Simi...


    pub_type: 杂志文章


    authors: Popik W,Pitha PM

    更新日期:1998-12-05 00:00:00

  • The insertion domain of the duck hepatitis B virus core protein plays a role in nucleocapsid assembly.

    abstract::Synthesis of hepadnaviral DNA is dependent upon both the viral DNA polymerase and the viral core protein, the subunit of the nucleocapsids in which viral DNA synthesis takes place. In a study of natural isolates of duck hepatitis B virus (DHBV), we cloned full-length viral genomes from a puna teal. One of the clones f...


    pub_type: 杂志文章


    authors: Guo H,Aldrich CE,Saputelli J,Xu C,Mason WS

    更新日期:2006-09-30 00:00:00

  • cDNAs of beet necrotic yellow vein virus RNAs 3 and 4 are rendered biologically active in a plasmid containing the cauliflower mosaic virus 35S promoter.

    abstract::cDNAs of beet necrotic yellow vein virus RNAs 3 and 4 could be rendered biologically active when they were placed under the control of the cauliflower mosaic virus 35S promoter and polyadenylation signal. Although the 35S in vivo transcripts should have contained up to forty 5' and several hundred 3' nonviral nucleoti...


    pub_type: 杂志文章


    authors: Commandeur U,Jarausch W,Li Y,Koenig R,Burgermeister W

    更新日期:1991-11-01 00:00:00

  • Domains in the simian immunodeficiency virus gp41 cytoplasmic tail required for envelope incorporation into particles.

    abstract::The mechanism by which lentivirus envelope (Env) glycoproteins are packaged into budding virions is poorly understood. Simian immunodeficiency virus (SIV) contains an Env protein with an unusually long cytoplasmic tail. To investigate the role of this domain in the incorporation of the SIV Env into virions, we generat...


    pub_type: 杂志文章


    authors: Celma CC,Manrique JM,Affranchino JL,Hunter E,González SA

    更新日期:2001-05-10 00:00:00

  • Endoplasmic reticulum export and vesicle formation of the movement protein of Chinese wheat mosaic virus are regulated by two transmembrane domains and depend on the secretory pathway.

    abstract::The 37K protein of Chinese wheat mosaic virus (CWMV) belongs to the 30K superfamily of plant virus movement proteins. CWMV 37K trans-complemented the cell-to-cell spread of a movement-defective Potato virus X. CWMV 37K fused to enhanced green fluorescent protein localized to plasmodesmata and formed endoplasmic reticu...


    pub_type: 杂志文章


    authors: Andika IB,Zheng S,Tan Z,Sun L,Kondo H,Zhou X,Chen J

    更新日期:2013-01-20 00:00:00

  • Molecular and serological characterization of SARS-CoV-2 infection among COVID-19 patients.

    abstract:BACKGROUND:SARS-CoV-2 is a novel coronavirus and the cause of COVID-19. More than 80% of COVID-19 patients exhibit mild or moderate symptoms. In this study, we investigated the dynamics of viral load and antibodies against SARS-CoV-2 in a longitudinal cohort of COVID-19 patients with severe and mild/moderate diseases. ...


    pub_type: 杂志文章


    authors: Li L,Liang Y,Hu F,Yan H,Li Y,Xie Z,Huang L,Zhao J,Wan Z,Wang H,Shui J,Cai W,Tang S

    更新日期:2020-12-01 00:00:00

  • Immunity to rotavirus in T cell deficient mice.

    abstract::Rotavirus infection was studied in adult nude mice (BALB/c background), alpha beta or gamma delta and alpha beta/gamma delta T cell receptor (TCR) knockout (-/-) mice (C57BL/6 and C57BL/6 x 129 backgrounds), and SCID mice (C57BL/6 background). The gamma delta TCR -/- mice cleared infection just like control mice. All ...


    pub_type: 杂志文章


    authors: Franco MA,Greenberg HB

    更新日期:1997-11-24 00:00:00

  • Effects of orientation and position on the activity of a herpes simplex virus immediate early gene far-upstream region.

    abstract::It has previously been shown that a far-upstream region of the herpes simplex virus immediate early (IE) gene 3 increases the expression of a linked thymidine kinase (TK) gene and also contains sequences which respond to stimulation of transcription by a virion component. The effects of altering the orientation and di...


    pub_type: 杂志文章


    authors: Preston CM,Tannahill D

    更新日期:1984-09-01 00:00:00

  • Comparing the functions of equine and canine influenza H3N8 virus PA-X proteins: Suppression of reporter gene expression and modulation of global host gene expression.

    abstract::The influenza PA-X protein is translated from the PA open reading frame from frameshifting and suppresses cellular gene expression due to its ribonuclease activity. We further defined the functional roles of PA-X by comparing PA-X proteins from two related viruses - equine influenza (EIV) and canine influenza (CIV) H3...


    pub_type: 杂志文章


    authors: Feng KH,Sun M,Iketani S,Holmes EC,Parrish CR

    更新日期:2016-09-01 00:00:00

  • The UL37 protein of herpes simplex virus type 1 is associated with the tegument of purified virions.

    abstract::The herpes simplex virus type 1 (HSV-1) UL37 open reading frame encodes a 120-kDa late (gamma 1) phosphoprotein in infected cells. Analysis of isolated mature HSV virions and light particles revealed that the UL37 protein is a component of the virion. Detergent solubilization and protease digestion experiments suggest...


    pub_type: 杂志文章


    authors: Schmitz JB,Albright AG,Kinchington PR,Jenkins FJ

    更新日期:1995-02-01 00:00:00

  • A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5.

    abstract::Early region 1 (E1) of the human adenoviruses has many intriguing properties which have prompted numerous mutational studies to help delineate and characterize the domains responsible for these functions. In mutational analyses being done currently, the E1 region is usually cloned into a bacterial plasmid where it is ...


    pub_type: 杂志文章


    authors: McGrory WJ,Bautista DS,Graham FL

    更新日期:1988-04-01 00:00:00

  • Semliki Forest virus produced in the absence of the 6K protein has an altered spike structure as revealed by decreased membrane fusion capacity.

    abstract::We examined the kinetics of membrane fusion of wild type (wt) and Delta6K mutant Semliki Forest virus in a liposomal model system. The final extent of membrane fusion of the mutant (at pH 5.5) was approximately one third that of the wt virus, although the level of E1 (fusion protein) trimerization was, in fact, greate...


    pub_type: 杂志文章


    authors: McInerney GM,Smit JM,Liljeström P,Wilschut J

    更新日期:2004-08-01 00:00:00

  • Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses.

    abstract::It is likely that phylogroup 2 lyssaviruses circulate within bat reservoirs. We adapted a pseudotype (pt) neutralisation assay (PNA) to a multiplex format enabling serosurveillance for Lagos bat virus (LBV), Mokola virus (MOKV) and West Caucasian bat virus (WCBV) in a potential reservoir, the African straw-coloured fr...


    pub_type: 杂志文章


    authors: Wright E,Hayman DT,Vaughan A,Temperton NJ,Wood JL,Cunningham AA,Suu-Ire R,Weiss RA,Fooks AR

    更新日期:2010-12-20 00:00:00

  • Crystalline cowpea mosaic virus.

    abstract::Crystals of Cowpea mosaic virus displaying a high degree of order have been grown in two different forms. Hexagonal crystals, space group P6(1)22 or P6(5)22 with a = 450 A and c = 1038 A containing six virus particles per cell, have been grown in sodium citrate buffer at pH 4.9. Each particle has four nearest neighbor...


    pub_type: 杂志文章


    authors: White JM,Johnson JE

    更新日期:1980-02-01 00:00:00

  • Varicella-zoster virus-specific gp140: a highly immunogenic and disulfide-linked structural glycoprotein.

    abstract::A 140,000-dalton disulfide-linked glycoprotein (gp140) specified by varicella-zoster virus (VZV) in infected cultured cells was identified and precipitated by two murine monoclonal antibodies (VZ-151 and VZ-158). When analyzed under reducing conditions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gp14...


    pub_type: 杂志文章


    authors: Grose C,Edwards DP,Weigle KA,Friedrichs WE,McGuire WL

    更新日期:1984-01-15 00:00:00

  • Restriction of Moloney murine leukemia virus replication in Moloney murine sarcoma virus-infected cells.

    abstract::MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...


    pub_type: 杂志文章


    authors: Yuen PH,Soong MM,Kissil MS,Wong PK

    更新日期:1984-01-30 00:00:00

  • A new and distinct species in the genus Caulimovirus exists as an endogenous plant pararetroviral sequence in its host, Dahlia variabilis.

    abstract::Viruses in certain genera in family Caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (EPRV). However, members of the genus Caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected ce...


    pub_type: 杂志文章


    authors: Pahalawatta V,Druffel K,Pappu H

    更新日期:2008-07-05 00:00:00

  • Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus.

    abstract::The genome of Lelystad virus (LV), a positive-strand RNA virus, is 15 kb in length and contains 8 open reading frames (ORFs) that encode putative viral proteins. ORFs 2 to 7 were cloned in plasmids downstream of the Sp6 RNA polymerase promoter, and the translation of transcripts generated in vitro yielded proteins tha...


    pub_type: 杂志文章


    authors: Meulenberg JJ,Petersen-den Besten A,De Kluyver EP,Moormann RJ,Schaaper WM,Wensvoort G

    更新日期:1995-01-10 00:00:00

  • RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs.

    abstract::Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various step...


    pub_type: 杂志文章


    authors: Huang F,Zhang J,Zhang Y,Geng G,Liang J,Li Y,Chen J,Liu C,Zhang H

    更新日期:2015-12-01 00:00:00

  • Autographa californica M nucleopolyhedrovirus ProV-CATH is activated during infected cell death.

    abstract::V-CATH, a cathepsin L-like cysteine protease encoded by the baculovirus Autographa californica M nucleopolyhedrovirus, has been shown to play an essential role in host liquefaction. Similar to cellular cathepsin L, V-CATH is synthesized as an inactive proenzyme and is activated by cleavage of the propeptide. Previous ...


    pub_type: 杂志文章


    authors: Hom LG,Ohkawa T,Trudeau D,Volkman LE

    更新日期:2002-05-10 00:00:00

  • Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection.

    abstract::Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber mo...


    pub_type: 杂志文章


    authors: Yan Y,Zhang B,Hou W,Lin H,Rebetz J,Hong SS,Wang Y,Ran L,Fan X

    更新日期:2017-05-01 00:00:00