Abstract:
:We have constructed the recombinant baculovirus which expresses the human immunodeficiency virus type 1 negative factor (nef) gene. Spodoptera frugiperda cells infected with the recombinant virus produced a 27-kDa protein which reacted with rabbit antisera raised against a carboxy-terminal synthetic peptide of the Nef protein by immunoblot analysis. Labeling experiment showed that the recombinant Nef protein was myristoylated. The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 4B, blue-Sepharose, and Sephadex G-150 column chromatography. No detectable GTP binding activity was observed in the purified recombinant Nef product.
journal_name
Virologyjournal_title
Virologyauthors
Matsuura Y,Maekawa M,Hattori S,Ikegami N,Hayashi A,Yamazaki S,Morita C,Takebe Ydoi
10.1016/0042-6822(91)90428-esubject
Has Abstractpub_date
1991-10-01 00:00:00pages
580-6issue
2eissn
0042-6822issn
1096-0341journal_volume
184pub_type
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