Abstract:
:Following challenge with COPV (canine oral papillomavirus), DNA plasmids encoding COPV L1, E1 or E2 protein were delivered into oral mucosal and cutaneous sites in beagles using particle-mediated immunotherapeutic delivery (PMID). Two weeks post-challenge, a priming dose of 8 microg DNA was delivered followed by a booster dose after a further two weeks. A group of control dogs were vaccinated using plasmid DNA encoding Hepatitis B virus surface (HBVs) gene. All of the control animals developed warts at the vast majority of sites (94%). All of the animals given wild type L1, E1, or E2 developed warts at most sites (88%, 75%, and 88%, respectively). The animals given codon optimised E2 however, were protected from wart growth with only one tiny lesion seen on a single animal that persisted for only a few days. The E1 codon optimised group was also significantly protected with a far lower number of smaller warts (48%) that persisted for a shorter duration. These data suggest that therapeutic immunisation by PMID with papillomavirus early genes is effective and emphasizes the importance of antigen load in the generation of protective responses to papillomavirus proteins.
journal_name
Virologyjournal_title
Virologyauthors
Moore RA,Walcott S,White KL,Anderson DM,Jain S,Lloyd A,Topley P,Thomsen L,Gough GW,Stanley MAdoi
10.1016/s0042-6822(03)00465-3subject
Has Abstractpub_date
2003-09-30 00:00:00pages
630-5issue
2eissn
0042-6822issn
1096-0341pii
S0042682203004653journal_volume
314pub_type
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