Emergence and epidemic occurrence of enterovirus 68 respiratory infections in The Netherlands in 2010.

abstract：:Trichomonas vaginalis viruses (TVV), which may regulate P270 gene expression in the protozoan pathogen T. vaginalis, are a group of divergent double-stranded (ds) RNA viruses. In the present study, the complete 4674-bp cDNA sequence of a 4.6-kb ds RNA from a newly identified TVV2-1 isolate was determined. The sequence...

journal_title：Virology

authors： Bessarab IN,Liu HW,Ip CF,Tai JH

更新日期：2000-02-15 00:00:00

abstract：:The movement protein (MP) of cauliflower mosaic virus (CaMV) is a multifunctional protein that potentiates the cell-to-cell and long distance movement of the virus. Functional domains in the CaMV MP were determined by analyzing deletions in green fluorescent protein (GFP)-MP fusions transfected into Arabidopsis thalia...

journal_title：Virology

authors： Huang Z,Han Y,Howell SH

更新日期：2000-05-25 00:00:00

abstract：:The human parvovirus B19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. So far, it has not been possible to grow B19 virus in cultured cells. Here we report the use of in vitro transcription in HeLa cell extracts and transient expression of cloned DNA transfected into HeLa ...

journal_title：Virology

authors： Doerig C,Beard P,Hirt B

更新日期：1987-04-01 00:00:00

abstract：:Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, has caused significant damage to the Asian and American pork industries. Coronavirus 3C-like protease (3CL(pro)), which is involved in the processing of viral polyproteins for viral replication, is an appealing antiviral drug target. Here,...

journal_title：Virology

authors： Ye G,Deng F,Shen Z,Luo R,Zhao L,Xiao S,Fu ZF,Peng G

更新日期：2016-07-01 00:00:00

abstract：:A mutant (dp 201) of human adenovirus type 2 has been isolated that has the left 3% (1110 bp) of the genome duplicated at the right terminus. The structure of the mutant genome was studied by restriction endonuclease analysis and by direct DNA sequence analysis. The duplication has resulted in a deletion of 10 bp of t...

journal_title：Virology

authors： Brusca JS,Chinnadurai G

更新日期：1983-09-01 00:00:00

abstract：:Missing contact footprinting with formic acid as a modifying reagent was used to examine specific IE-1 binding contacts to double-stranded oligonucleotides that contained either a consensus hr repeat sequence or a sequence from the pe38 promoter, which is down regulated by IE-1. The hr repeat sequences contain two con...

journal_title：Virology

authors： Leisy DJ,Rohrmann GF

更新日期：2000-08-15 00:00:00

abstract：:Serial passage of Ross River virus in chick embryo fibroblasts selected for virus variants altered in their reactions to neutralizing monoclonal antibodies. The E1 and E2 genes of each antigenic variant were sequenced; single nucleotide changes were found in E2 leading to amino acid substitutions at either residue 4 (...

journal_title：Virology

authors： Kerr PJ,Weir RC,Dalgarno L

更新日期：1993-03-01 00:00:00

abstract：:Nipah virus (NV) and Hendra virus (HV) are recently emergent, related viruses that can cause severe disease in humans and animals. The goal of this study was to investigate the immunogenic and functional properties of the fusion (F) and attachment (G) glycoproteins of NV. Vaccination of mice with recombinant vaccinia ...

journal_title：Virology

authors： Tamin A,Harcourt BH,Ksiazek TG,Rollin PE,Bellini WJ,Rota PA

更新日期：2002-04-25 00:00:00

abstract：:Natural infection and vaccination with a live-attenuated measles virus (MV) induce CD8(+) T-cell-mediated immune responses that may play a central role in controlling MV infection. In this study, we show that newly identified human HLA-A2 epitopes from MV hemagglutinin (H) and fusion (F) proteins induced protective im...

journal_title：Virology

authors： Oh S,Stegman B,Pendleton CD,Ota MO,Pan CH,Griffin DE,Burke DS,Berzofsky JA

更新日期：2006-09-01 00:00:00

abstract：:Norwalk virus (NV) is the prototype strain of a group of noncultivatable caliciviruses that infect humans and cause outbreaks of epidemic acute nonbacterial gastroenteritis. The NV virion is composed of 180 copies of a single structural protein that, when expressed in insect cells infected with a recombinant baculovir...

journal_title：Virology

authors： Hardy ME,Tanaka TN,Kitamoto N,White LJ,Ball JM,Jiang X,Estes MK

更新日期：1996-03-01 00:00:00

abstract：:An arginine-rich RNA-binding motif (ARM) found at the N-proximal region of Brome mosaic virus (BMV) coat protein (CP) adopts alpha-helical conformation and shares homology with CPs of plant and insect RNA viruses, HIV-Rev and Tat proteins, bacterial antiterminators, and ribosomal splicing factors. The ARM of BMV CP, c...

journal_title：Virology

authors： Choi YG,Rao AL

更新日期：2000-09-15 00:00:00

abstract：:Rep78/68 proteins of adeno-associated virus type 2 (AAV-2) are involved in many aspects of the viral life cycle, including replication, gene expression, and site-specific integration. To understand the molecular mechanisms of the actions of Rep proteins, we searched for Rep68-interacting cellular proteins by utilizing...

journal_title：Virology

authors： Han SI,Kawano MA,Ishizu K,Watanabe H,Hasegawa M,Kanesashi SN,Kim YS,Nakanishi A,Kataoka K,Handa H

更新日期：2004-03-01 00:00:00

abstract：:Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and g...

journal_title：Virology

authors： Lawrence P,Pacheco JM,Uddowla S,Hollister J,Kotecha A,Fry E,Rieder E

更新日期：2013-02-05 00:00:00

abstract：:Bovine herpesvirus 5 (BHV-5) is a neurovirulent alpha-herpesvirus that causes fatal encephalitis in calves. We previously demonstrated that deletion of a glycine-rich epitope in the gE ectodomain dramatically reduced BHV-5 neurovirulence. To investigate the role of gE cytoplasmic tail sequences in the neuropathogenesi...

journal_title：Virology

authors： Al-Mubarak A,Simon J,Coats C,Okemba JD,Burton MD,Chowdhury SI

更新日期：2007-09-01 00:00:00

abstract：:Activation of the human cytomegalovirus (HCMV) origin of replication (oriLyt) was previously demonstrated in transient transfection assays in permissive human fetal fibroblasts and nonpermissive Vero cells, and shown to require six viral proteins that function at the replication fork plus a number of HCMV products tha...

journal_title：Virology

authors： Reid GG,Ellsmore V,Stow ND

更新日期：2003-04-10 00:00:00

abstract：:Previously, an epitope recognized by a set of neutralizing monoclonal antibodies directed against the S protein of transmissible gastroenteritis has been identified. This neutralization epitope can be simulated by a single peptide combining residues 380 to 387 and 1176 to 1184 of the S protein; this combination peptid...

journal_title：Virology

authors： Posthumus WP,Lenstra JA,van Nieuwstadt AP,Schaaper WM,van der Zeijst BA,Meloen RH

更新日期：1991-05-01 00:00:00

abstract：:The nonstructural protein expressed from ORF-206 of turnip yellow mosaic virus is proteolytically processed to produce N-terminal 150-kDa and C-terminal 70-kDa proteins. Through the use of linker insertion and deletion analyses coupled to in vitro translation, we have delimited the protease domain to residues 731-885 ...

journal_title：Virology

authors： Bransom KL,Dreher TW

更新日期：1994-01-01 00:00:00

abstract：:The transcriptive complex, the nucleocapsid with the viral RNA-synthesizing activity, of Newcastle disease virus (NDV) contains three protein components, the major structural subunit (NP) and two associated proteins (P and L) involved in the RNA synthesis. We studied the pathway of these proteins from synthesis to ass...

journal_title：Virology

authors： Hamaguchi M,Nishikawa K,Toyoda T,Yoshida T,Hanaichi T,Nagai Y

更新日期：1985-12-01 00:00:00

abstract：:An ideal system to investigate individual determinants of the replication process of (+)-strand RNA viruses is a cell-free extract that supports viral protein and RNA synthesis in a synchronized manner. Here, we applied a translation/replication system based on cytoplasmic extracts of Nicotiana tabacum cells to Tomato...

journal_title：Virology

authors： Gursinsky T,Schulz B,Behrens SE

更新日期：2009-08-01 00:00:00

abstract：:The temperate Streptococcus thermophilus bacteriophage TP-J34 was identified in the lysogenic host strain J34. The majority of phage particles produced upon induction was defective and noninfectious, consisting of DNA-filled heads lacking tails. A physical map (45.6 kb) was established. Analysis of minor restriction b...

journal_title：Virology

authors： Neve H,Freudenberg W,Diestel-Feddersen F,Ehlert R,Heller KJ

更新日期：2003-10-10 00:00:00

abstract：:The biological phenotype of HIV-1 isolates from 21 subjects with primary HIV-1 infection was determined in primary lymphocytes and monocyte-derived macrophages as well as in three T-cell lines (MT-2, HUT78, and C8166). Seven isolates (33%) replicated on at least one cell line and were classified as rapid/high (n = 6) ...

journal_title：Virology

authors： Fiore JR,Björndal A,Peipke KA,Di Stefano M,Angarano G,Pastore G,Gaines H,Fenyö EM,Albert J

更新日期：1994-10-01 00:00:00

abstract：:Repression of human papillomavirus (HPV) E6 and E7 oncogenes in established cervical carcinoma cell lines causes senescence due to reactivation of cellular tumor suppressor pathways. Here, we determined whether ongoing expression of HPV16 or HPV18 oncogenes is required for the proliferation of primary human cervical c...

journal_title：Virology

authors： Magaldi TG,Almstead LL,Bellone S,Prevatt EG,Santin AD,DiMaio D

更新日期：2012-01-05 00:00:00

abstract：:While trying to generate a site-directed deletion in the ORF63 latency-associated gene of varicella-zoster virus (VZV) Oka, we constructed a virus with an unexpected rearrangement. The virus has a small deletion in both copies of ORF63 and two copies of a cassette inserted between ORFs 64/65 and 68/69 containing (a) t...

journal_title：Virology

authors： Ambagala AP,Krogmann T,Qin J,Pesnicak L,Cohen JI

更新日期：2010-04-10 00:00:00

abstract：:The DNAs from the granulosis viruses of Plodia interpunctella and Pieris rapae were clearly distinguishable based on restriction endonuclease fragment patterns. By sizing single- and double-digestion products approximate molecular weights of 72 x 10(2) and 75 x 10(6), respectively, were estimated for the viral genomes...

journal_title：Virology

authors： Tweeten KA,Bulla LA Jr,Consigli RA

更新日期：1980-07-30 00:00:00

abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of natura...

journal_title：Virology

authors： Goldberg TL,Lowe JF,Milburn SM,Firkins LD

更新日期：2003-12-20 00:00:00

abstract：:The 5' leader region of the HIV-1 RNA contains the major 5' splice site (ss) that is used in the production of all spliced viral RNAs. This splice-donor (SD) region can fold a stem-loop structure. We demonstrate that whereas stabilization of this SD hairpin reduces splicing efficiency, destabilization increases splici...

journal_title：Virology

authors： Mueller N,van Bel N,Berkhout B,Das AT

更新日期：2014-11-01 00:00:00

abstract：:The role of anti-viral antibodies in homologous protective immunity to a virulent African swine fever virus (ASFV) strain E75 was examined by passive transfer experiments in swine. Eighty-five percent of animals (n = 14) that received anti-ASFV immunoglobulin (Ig) survived challenge infection, while 100% mortality was...

journal_title：Virology

authors： Onisk DV,Borca MV,Kutish G,Kramer E,Irusta P,Rock DL

更新日期：1994-01-01 00:00:00

abstract：:Little is known concerning the function of the C-terminal nonconserved region of Sindbis virus nsP3. In this report, we created a number of in-frame deletions and duplications (from 12 to 159 residues) in this region and examined their effects on Sindbis virus replication. Sindbis RNA transcripts containing these muta...

journal_title：Virology

authors： Lastarza MW,Grakoui A,Rice CM

更新日期：1994-07-01 00:00:00

abstract：:An SH3-binding domain within the Nef protein of primate lentiviruses has been reported to be important to viral replication and infectivity and dispensable for CD4 downregulation, but its precise role remains unclear. This study investigates the effects of mutations in both the polyproline helix and in the hydrophobic...

journal_title：Virology

authors： Craig HM,Pandori MW,Riggs NL,Richman DD,Guatelli JC

更新日期：1999-09-15 00:00:00

abstract：:Lysolecithin permeabilization of vaccinia virus-infected cells was employed to prepare extracts that support faithful transcription initiation in vitro on plasmids possessing early, intermediate, and late viral gene promoters. Conditions which optimize transcription from each promoter were defined. The in vitro system...

journal_title：Virology

authors： Condit RC,Lewis JI,Quinn M,Christen LM,Niles EG

更新日期：1996-04-01 00:00:00