Abstract:
:A mutant (dp 201) of human adenovirus type 2 has been isolated that has the left 3% (1110 bp) of the genome duplicated at the right terminus. The structure of the mutant genome was studied by restriction endonuclease analysis and by direct DNA sequence analysis. The duplication has resulted in a deletion of 10 bp of the right inverted terminal repeat (ITR) at the junction of right and left end sequences. This internal ITR is not active in initiation or termination of DNA synthesis. Two subclones of dp 201 that bear a deletion between map positions 2.1 and 6.8 encompassing early gene blocks E1a and E1b were also isolated. One of these isolates (dl 201.1) retains the left end duplication and the other isolate (dl 201.2) lacks the left end duplication and is an apparent revertant of dl 201.1. The original mutant dp 201 also reverts to a wild type sequence at a low rate. Since 10 base pairs of the right ITR are deleted in the mutant, reversion must involve a repair mechanism using one of the two complete left end ITRs in the mutant genome. The mechanisms by which this reversion may occur are discussed.
journal_name
Virologyjournal_title
Virologyauthors
Brusca JS,Chinnadurai Gdoi
10.1016/0042-6822(83)90177-0subject
Has Abstractpub_date
1983-09-01 00:00:00pages
381-92issue
2eissn
0042-6822issn
1096-0341journal_volume
129pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Metagenomic analysis of whole mosquitoes allows the genetic characterization of all associated viruses, including arboviruses and insect-specific viruses, plus those in their diet or infecting their parasites. We describe here the virome in mosquitoes, primarily Culex pipiens complex, Cx. tarsalis and Cx. erythrothora...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.07.029
更新日期:2018-10-01 00:00:00
abstract::ZEBRA, a transcription factor and DNA replication protein encoded by the Epstein-Barr virus (EBV) BZLF1 gene, plays indispensable roles in the EBV lytic cycle. We recently described the phenotypes of 46 single amino acid substitutions introduced into the DNA-recognition region of ZEBRA [Heston, L., El-Guindy, A., Coun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.009
更新日期:2008-12-20 00:00:00
abstract::Cucumber mosaic virus (CMV), generated from biologically active cDNA clones of Fny-CMV RNA 1 plus 2 and Sny-CMV RNA 3, derived from the Fny- and Sny-strains of CMV, was able to infect tobacco but not squash plants systemically. In squash, viral RNA, movement protein, and coat protein all accumulated in the inoculated ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8468
更新日期:1997-04-14 00:00:00
abstract::Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in con...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1200
更新日期:1995-04-20 00:00:00
abstract::Poliovirus replicons were constructed which contain the internal ribosome entry site (IRES) of encephalomyocarditis virus (EMCV) substituted for the poliovirus IRES. To monitor gene expression and encapsidation, the gene encoding firefly luciferase was substituted for the P1 gene. Replicons can be encapsidated followi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0433
更新日期:2000-08-01 00:00:00
abstract::The bottom component B1a of TYMV with a buoyant density of 1.42-1.43 g/ml, is converted to a more dense particle B2a following CsCI density gradient centrifugation. This conversion is complete after a prior incubation of the virus in the CsCl solution at elevated temperatures. It is shown that the formation of B28 is ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90041-1
更新日期:1982-07-30 00:00:00
abstract::Human cytomegalovirus (HCMV) infects the chorioamnion, but whether these infections cause fetal membrane dysfunction remains poorly understood. We sought to assess whether guinea pig cytomegalovirus (GPCMV) infects amnion-derived cells in vitro, compare the inflammatory response of amnion cells to GPCMV and HCMV, and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.06.005
更新日期:2020-09-01 00:00:00
abstract::Following mechanical inoculation of leaves with turnip yellow mosaic virus (TYMV), a significant proportion of the retained inoculum is uncoated within 45 sec, and the process is more or less complete after 2 min. At least 80-90% of the uncoating takes place in the epidermis. The application of virus to the intact lea...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90274-0
更新日期:1985-07-30 00:00:00
abstract::Glycoprotein B (gB, gpUL55) is a major antigen for the induction of neutralizing antibodies against human cytomegalovirus, making it an attractive antigen for active and passive immunoprophylaxis. The immunodominant region on gB is the antigenic domain 1 (AD-1), a complex structure which requires a minimal linear amin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0040
更新日期:1996-02-01 00:00:00
abstract::The 2009 influenza pandemic originated from a swine-origin H1N1 virus, which, although less pathogenic than anticipated, may acquire additional virulence-associated mutations in the future. To estimate the potential risk, we sequentially passaged the isolate A/Hamburg/04/2009 in A549 human lung epithelial cells. After...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.02.002
更新日期:2016-05-01 00:00:00
abstract::Purified preparations of the Helminthosporium victoriae 190S (Hv190S) virus contain two sedimenting components that differ in capsid structure. The slower sedimenting component (190S-1) contained p88 and p83 as the major capsid proteins; the faster component (190S-2) contained p88 and p78. Previous peptide-mapping stu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90520-y
更新日期:1992-06-01 00:00:00
abstract::Identification of regulators of p53 expression is a crucial step in understanding the diverse functions of p53 and its role in cellular homeostasis and responsiveness to insult. Several viral proteins inactivate p53 as a modulator of cell cycle progression and apoptosis. Here, we report that a unique leporipoxviral tr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0507
更新日期:1996-10-01 00:00:00
abstract::At high concentrations, several monoclonal antibodies to the G1 glycoprotein of La Crosse (LAC) virus aggregated the virus. To determine whether this accounted for the neutralization, the monoclonal antibodies were digested to make Fab fragments. With one exception, each monovalent antibody neutralized LAC virus to th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90073-k
更新日期:1991-02-01 00:00:00
abstract::During replication of human parainfluenza virus type 3 (HPIV3), the 96-nucleotide antigenomic promoter (AGP) of HPIV3 directs the synthesis of genomic RNA. Previous work showed that nucleotides 1-12 were critical in promoting replication of an HPIV3 minireplicon, but nucleotides 13-96 were not investigated. In this st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.08.003
更新日期:2011-10-25 00:00:00
abstract::Classical swine fever virus (CSFV) is a positive-sense RNA virus with a high degree of genetic variability among isolates. High diversity is also found in virulence, with strains covering the complete spectrum from avirulent to highly virulent. The underlying genetic determinants are far from being understood. Since R...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.020
更新日期:2013-03-30 00:00:00
abstract::The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant wer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.04.007
更新日期:2009-06-20 00:00:00
abstract::Infection with the Tucson strain of coxsackievirus B1 (CVB1T) causes the development of chronic inflammatory myopathy (CIM) and hind limb weakness in susceptible strains of mice. In this study, a panel of six plaque-purified viruses exhibiting either small or large plaque phenotypes was derived from parental CVB1T and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8592
更新日期:1997-06-23 00:00:00
abstract::The core gene promoter of duck hepatitis B virus (DHBV) has been localized and an enhancer element has been found in a region of the DHBV genome immediately upstream of the core promoter. This enhancer was able to activate expression from both the core promoter and the S promoter of DHBV, as well as from the heterolog...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90841-x
更新日期:1991-09-01 00:00:00
abstract::Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5 degrees. I...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90139-3
更新日期:1983-10-15 00:00:00
abstract::Previous reports from our lab had shown that sera obtained from SIV(mac)-infected animals neutralized SIV(mac) infectivity in CD4(+) T cells but failed to protect monkey primary macrophages from infection with the virus. However, the antibodies could inhibit completion of the viral life cycle in the macrophages at the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1053
更新日期:2001-09-01 00:00:00
abstract::Endogenous feline leukemia viruses (enFeLVs) occur in the germ lines of the domestic cat and related wild species (genus Felis). We sequenced the long terminal repeats and part of the env region of enFeLVs in domestic cats and five wild species. A total of 305 enFeLV sequences were generated across 17 individuals, dem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.010
更新日期:2010-09-30 00:00:00
abstract::Cardioviruses cause diseases in many animals including, in rare cases, humans. Although they share common features with all picornaviruses, cardioviruses have unique properties that distinguish them from other family members, including enteroviruses. One feature shared by all picornaviruses is the covalent attachment ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.01.010
更新日期:2018-03-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) is a widespread pathogen that persists for life, replicating in surface tissues and establishing latency in peripheral ganglia. Increasingly, molecular studies of latency use cultured neuron models developed using recombinant viruses such as HSV-1 GFP-US11, a derivative of strain Patton ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.09.016
更新日期:2017-12-01 00:00:00
abstract::Human Adenovirus Type 4 (HAdV-4) is responsible for epidemic outbreaks of Acute Respiratory Disease (especially in military recruits), and is known to cause significant morbidity with several reported cases of mortality. However, we do not understand why this serotype causes such high morbidity, and have little insigh...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.01.017
更新日期:2008-05-10 00:00:00
abstract::A number of factors that affect genetic interaction during mixed infection with temperature-sensitive mutants of simian rotavirus SA11 have been examined. (1) Statistical analyses of recombination frequency (RF) indicated that (a) the variability noted in RF was not related to variations in experimental conditions and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90374-4
更新日期:1983-05-01 00:00:00
abstract::The major outer capsid protein, VP5, of five US bluetongue viruses, was found to be glycosylated. Enzymatic removal of the carbohydrate moiety did not affect the electrophoretic mobility of VP5 in SDS-PAGE, indicating the presence of only short and possibly unbranched oligosaccharide chains. The surface accessibilitie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(83)90000-4
更新日期:1993-05-01 00:00:00
abstract::The Epstein-Barr virus open reading frame BLLF1 encodes the major envelope glycoproteins gp350 and gp220. Fragments of the gp350/220 gene were expressed in Escherichia coli in order to define regions of the polypeptide chain reacting with human sera. The C-terminal half of the protein was sufficient for recognition by...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90207-6
更新日期:1992-11-01 00:00:00
abstract::Highly pathogenic alphaviruses display complex glycans on their surface. These glycans play a crucial role in viral pathogenesis by facilitating glycan-host interaction during viral entry which can be targeted. Various studies have reported antiviral activity of lectins that bind to the glycans present on the surface ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.10.009
更新日期:2019-01-02 00:00:00
abstract::The M128L myxoma virus gene expresses a five-membrane spanning cell surface protein with significant amino acid homology to the cellular CD47 proteins. CD47, also called integrin-associated protein (IAP), is associated with the modulation of leukocyte adhesion, motility, activation, and phagocytosis. Creation of an M1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.03.037
更新日期:2005-06-20 00:00:00
abstract::The gene encoding the VP8* trypsin-cleavage product of the VP4 protein of porcine rotavirus strain A34 was sequenced, and the predicted amino acid (aa) sequence was compared to the homologous region of all known P genotypes. The aa sequence of the VP8* of strain A34 shared low identity, ranging from 39% (bovine strain...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00534-8
更新日期:2003-10-25 00:00:00