Abstract:
:During replication of human parainfluenza virus type 3 (HPIV3), the 96-nucleotide antigenomic promoter (AGP) of HPIV3 directs the synthesis of genomic RNA. Previous work showed that nucleotides 1-12 were critical in promoting replication of an HPIV3 minireplicon, but nucleotides 13-96 were not investigated. In this study, the role of nucleotides 13-96 in AGP function was analyzed by creating and assaying mutations in an HPIV3 minireplicon. A replication promoting element known as promoter element II (nt 79-96) was confirmed in the HPIV3 AGP. Additionally, nucleotides 13-39 were found to constitute an additional positive-acting cis-element. However, detailed analysis of the 13-39 element revealed a complicated control element with both stimulatory and repressing elements. Specifically, nucleotides 21-28 were shown to repress RNA replication, while flanking sequences had a stimulatory effect.
journal_name
Virologyjournal_title
Virologyauthors
Gander JR,Schwan LM,Hoffman MAdoi
10.1016/j.virol.2011.08.003subject
Has Abstractpub_date
2011-10-25 00:00:00pages
90-6issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(11)00345-Xjournal_volume
419pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The CC chemokine receptor (CCR) 7 ligands CCL21 and CCL19 were recently described as essential elements for establishing the microenvironment needed to initiate optimal immune responses in secondary lymphoid tissues. In the present study we have kinetically investigated the primary responses of naive DO11.10 TCR-trans...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00199-5
更新日期:2003-07-20 00:00:00
abstract::In 1943 Burnet reported on changes in the hemagglutinating properties of human influenza virus which occurred during adaptation of the virus to growth in chicken eggs. Only recently has direct evidence been presented that these changes affect the antigenic properties of the virus. Schild et al. (G. C. Schild, J. S. Ox...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90105-9
更新日期:1985-05-01 00:00:00
abstract::Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.06.025
更新日期:2011-09-01 00:00:00
abstract::Immunization with heat-inactivated herpes simplex virus type 1 (HSV-1) 2-5 days before ocular infection reduced the frequency of establishment of latent HSV-1 infection in the trigeminal ganglion (TG); this induction of resistance coincided with reduced expression of IFN-gamma mRNA in the TG. Immunization with unrelat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8738
更新日期:1997-09-29 00:00:00
abstract::Adenovirus-mediated virotherapy is one of the promising therapeutic approaches for glioma treatment. However, its replication efficiency and specificity still failed to meet the requirements for clinical treatment. To improve the anti-tumor activity and specificity of oncolytic adenoviruses (OA), we applied multiple m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.04.007
更新日期:2014-06-01 00:00:00
abstract::Human adenovirus (HAdV) is a cause of significant morbidity and mortality in immunocompromised patients, especially after stem cell transplantation (SCT). Viral clearance has been attributed to CD4(+) T-cell responses against the Hexon-protein, but the frequency of specific T(HELPER) cells is extremely low or not dete...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.049
更新日期:2010-02-20 00:00:00
abstract::A system to associate specific genome segments with viral phenotypes and to study factors influencing genome reassortment was developed for tomato spotted wilt Tospovirus (TSWV). Reassortant isolates were generated by co-inoculating a TSWV isolate, TSWV-D, with TSWV-10 or TSWV-MD. The parental origin of each genome se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9131
更新日期:1998-04-25 00:00:00
abstract::The failure of HIV-1 to escape at some cytotoxic T-lymphocyte (CTL) epitopes has generally been explained in terms of viral fitness costs or ineffective or attenuated CTL responses. Relatively little attention has been paid to the evolutionary time required for escape mutants to be detected. This time is significantly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.036
更新日期:2006-03-30 00:00:00
abstract::Neutralization epitopes of duck hepatitis B virus (DHBV) have been previously mapped within the N-terminal portion of the pre-S protein using monoclonal antibodies. However, the immune response of ducks to this region is not well characterized at the amino acid level. To this end, we have immunized adult Pekin ducks w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1164
更新日期:1994-04-01 00:00:00
abstract::A viroid has been purified from avocado leaves infected by sunblotch disease and designated the avocado sunblotch viroid. It is a covalently closed circular RNA molecule with a molecular weight lower than that of chrysanthemum stunt viroid and citrus exocortis viroid while hybridization analysis with 32P-labeled compl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90045-x
更新日期:1979-11-01 00:00:00
abstract::We used serial protein misfolding cyclic amplification (sPMCA) to amplify the D10 strain of CWD prions in a linear relationship over two logs of D10 dilutions. The resultant PMCA-amplified D10 induced terminal TSE disease in CWD-susceptible Tg(cerPrP)1536 mice with a survival time approximately 80 days shorter than th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.023
更新日期:2008-12-20 00:00:00
abstract::Within immunocompromised populations, the JC polyomavirus is the cause of the often-fatal disease Progressive Multifocal Leukoencephalopathy (PML). JC virus encodes a protein, termed T-antigen (T-ag), which is essential for its replication and pathogenicity. Previous studies of JCV T-ag have, in general, used antibodi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.006
更新日期:2016-10-01 00:00:00
abstract::Classical swine fever virus (CSFV) nonstructural protein NS5A is a multifunctional protein functioning in regulation of viral genome replication, protein translation and assembly by interaction with viral or host proteins. Here, heat shock protein 27 (Hsp27) has been identified as a novel binding partner of NS5A by us...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.02.020
更新日期:2018-05-01 00:00:00
abstract::Hepatitis delta virus (HDV) encodes only one protein, the hepatitis delta antigen (HDAg). Two forms of HDAg, a large (27 kDa) and a small (24 kDa) one, participate in the various steps of HDV replication. To further understand the properties of HDAg, we have constructed recombinant baculoviruses and expressed both for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91227-l
更新日期:1992-09-01 00:00:00
abstract::Puumala (PUUV) and Hantaan (HTNV) viruses are hantaviruses within the family Bunyaviridae and associated with Hemorrhagic Fever with Renal Syndrome (HFRS) in humans. Little is known about how these viruses interact with host cells, though pathogenic hantaviruses interact with α(v)β(3) integrin. To study host cell inte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.08.012
更新日期:2012-02-20 00:00:00
abstract::The 130-kDa bovine herpesvirus-1 (BHV-1) glycoprotein GVP 6 was found to cross-react immunologically with the herpes simplex glycoprotein gB. Antibodies in polyclonal serum against gB immunoprecipitated GVP 6 and its cleavage products from a lysate of BHV-1-infected cells. Conversely, polyclonal serum against GVP 6 pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90525-9
更新日期:1988-10-01 00:00:00
abstract::In retrovirus-induced tumors, proviral DNA is commonly found next to or within a cellular proto-oncogene such that transcription of the gene is influenced by the viral promoter or enhancer. Extensive surveys of naturally occurring tumors reveal that proviruses integrated on the 3' side of the gene are usually in the s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1245
更新日期:1993-05-01 00:00:00
abstract::Five regions on the coat protein of BNYVV which had been shown previously to be involved in the formation of continuous epitopes were further analyzed by means of synthetic overlapping peptides. It was found that at least some of these regions may encompass several overlapping epitopes (or parts thereof). Four monoclo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1031
更新日期:1994-01-01 00:00:00
abstract::Tomato strain L of tobacco mosaic virus (TMV-L) induces a hypersensitive response (necrotic local lesions) on tobacco plants with the N' gene. A factor responsible for induction of the hypersensitive response has been mapped to the coat protein gene. We have constructed several mutants which have insertions or deletio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90217-1
更新日期:1989-11-01 00:00:00
abstract::Many viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereby modulating diverse downstream signaling pathways associated with antiapoptosis, proliferation, cell cycling, protein synthesis and glucose metabolism, in order to augment their replication. To date, the role of the PI3K-Akt p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.007
更新日期:2009-08-15 00:00:00
abstract::Human papillomavirus type 6 (HPV 6) DNA was detected in an episomal form in DNA extracted from an invasive squamous carcinoma of the vulva. The viral DNA, designated HPV6-T70, was molecularly cloned. Restriction analysis of the HPV6-T70 genome revealed an insertion of approximately 35 bp in the 5' portion of the upstr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90676-9
更新日期:1988-07-01 00:00:00
abstract::Nipah virus (NV) and Hendra virus (HV) are recently emergent, related viruses that can cause severe disease in humans and animals. The goal of this study was to investigate the immunogenic and functional properties of the fusion (F) and attachment (G) glycoproteins of NV. Vaccination of mice with recombinant vaccinia ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1418
更新日期:2002-04-25 00:00:00
abstract::The equine herpesvirus 1 (EHV-1) immediate-early (IE) phosphoprotein is essential for the activation of transcription from viral early and late promoters and regulates transcription from its own promoter. The IE protein of 1487 amino acids contains a serine-rich tract (SRT) between residues 181 and 220. Deletion of th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0725
更新日期:2001-01-05 00:00:00
abstract::Expression of foreign peptides on the surface of cowpea mosaic virus particles leads to the creation of chimaeras with a variety of phenotypes and yields. Two factors were shown to be particularly significant in determining the properties of a given chimaera: the length of the inserted sequence and its isoelectric poi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00140-5
更新日期:2003-05-25 00:00:00
abstract::The liver has been suspected to be one of the major targets of dengue virus infection. Here, we detected increasing secretion of the chemokine RANTES (regulated upon activation, normal T cell expressed and secreted), which functions to recruit the immune cells, in dengue-virus-infected liver cells and patients. Three ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0524
更新日期:2000-10-10 00:00:00
abstract::The mechanism of vaccinia virus-induced selective inhibition of host cell protein synthesis was studied in a nonpermissive (Chinese hamster ovary, CHO) and in a permissive mouse cell line ( L cells). Small polyadenylated RNAs obtained from uninfected and infected cells were fractionated into six size classes by polyac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90135-e
更新日期:1990-12-01 00:00:00
abstract::The extent of viral DNA methylation in non-virus-producing benign and malignant tumors induced by the Shope (rabbit) papilloma virus was investigated with the two isoschizomeric restriction endonucleases MspI and HpaII. The overall extent of methylation is variable in papillomas and uniformly higher in carcinomas. In ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(83)80007-5
更新日期:1983-04-30 00:00:00
abstract::In this study, we show that adenoviral infection induced accumulation of the sphingolipid ceramide in a dose- and time-dependent manner. This accumulation preceded cell lysis, occurred in the absence of biochemical evidence of apoptosis, and was derived from de novo synthesis of ceramide. An adenovirus mutant that lac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.09.060
更新日期:2006-02-05 00:00:00
abstract::We have used a yeast two-hybrid system to show that human papillomavirus E7 proteins can form oligomeric complexes in vivo. The carboxyl-terminal cysteine-rich metal-binding domain is critical for this activity although amino-terminal sequences also contribute to oligomerization. Our experiments also reveal that E7 po...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.9926
更新日期:1995-12-01 00:00:00
abstract::The guinea pig cytomegalovirus (GPCMV) co-linear gene and potential functional homolog of HCMV UL84 (GP84) was investigated. The GP84 gene had delayed early transcription kinetics and transient expression studies of GP84 protein (pGP84) demonstrated that it targeted the nucleus and co-localized with the viral DNA poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.10.028
更新日期:2011-02-05 00:00:00