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Cellular Hsp27 interacts with classical swine fever virus NS5A protein and negatively regulates viral replication by the NF-κB signaling pathway.

Abstract：

:Classical swine fever virus (CSFV) nonstructural protein NS5A is a multifunctional protein functioning in regulation of viral genome replication, protein translation and assembly by interaction with viral or host proteins. Here, heat shock protein 27 (Hsp27) has been identified as a novel binding partner of NS5A by using His tag "pull down" coupled with shotgun LC-MS/MS, with interaction of both proteins further confirmed by co-immunoprecipitation and laser confocal assays. In PK-15 cells, silencing of Hsp27 expression by siRNA enhanced CSFV replication, and upregulation of Hsp27 inhibited viral proliferation. Additionally, we have shown that overexpression of Hsp27 increased NF-κB signaling induced by TNFα. Blocking NF-κB signaling in PK-15 cells overexpressing Hsp27 by ammonium pyrrolidinedithiocarbamate (PDTC) eliminated the inhibition of CSFV replication by Hsp27. These findings clearly demonstrate that the inhibition of CSFV replication by Hsp27 is mediated via the NF-κB signaling pathway.

journal_name

Virology

journal_title

Virology

authors

Ling S,Luo M,Jiang S,Liu J,Ding C,Zhang Q,Guo H,Gong W,Tu C,Sun J

doi

10.1016/j.virol.2018.02.020

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

202-209

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(18)30064-3

journal_volume

518

pub_type

杂志文章

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