当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > The most frequent naturally occurring length polymorphism in the HIV-1 LTR has little effect on proviral transcription and viral replication.

The most frequent naturally occurring length polymorphism in the HIV-1 LTR has little effect on proviral transcription and viral replication.

Abstract：

:About 38% of primary HIV-1 long terminal repeats (LTRs) contain an insertion (consensus: 5prime prime or minute-ACYGCTGA-3prime prime or minute), termed the most frequent naturally occurring length polymorphism (MFNLP). The MFNLP binds several transcription factors and might affect HIV-1 replication and disease progression in infected individuals. However, its relevance for proviral transcription and for HIV-1 replication in primary cells is unclear. We utilized HIV-1 NL4-3 LTR variants to investigate the effect of the MFNLP on 5prime prime or minuteLTR transcriptional activity in various cell types. Notably, viral promoter activity was studied in primary cells in the context of the integrated provirus, using both single cycle assays with pseudotyped Luciferase reporter viruses and replication-competent HIV-1 mutants. Our results demonstrate that the presence, absence, or duplication of the 5prime prime or minute-ACYGCTGA-3prime prime or minute motif has little effect on viral promoter activity in T cell lines, peripheral blood mononuclear cells (PBMC), and monocyte-derived macrophages (MDM). Furthermore, all HIV-1 LTR variants showed efficient induction upon stimulation with TPA and/or ionomycin and replicated with comparable efficiency in a human T cell line and in PBMC. Thus, the MFNLP does not significantly affect HIV-1 5prime prime or minuteLTR transcriptional activity and viral replication in primary cells, suggesting that this common sequence variation has little impact on the clinical course of HIV-1 infection.

journal_name

Virology

journal_title

Virology

authors

Hiebenthal-Millow K,Kirchhoff F

doi

10.1006/viro.2001.1282

subject

Has Abstract

pub_date

2002-01-05 00:00:00

pages

169-75

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042682201912826

journal_volume

292

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Structural characterization of the PCV2d virus-like particle at 3.3 Å resolution reveals differences to PCV2a and PCV2b capsids, a tetranucleotide, and an N-terminus near the icosahedral 3-fold axes.

    abstract：:Porcine circovirus 2 (PCV2) has a major impact on the swine industry. Eight PCV2 genotypes (a-h) have been identified using capsid sequence analysis. PCV2d has been designated as the emerging genotype. The cryo-electron microscopy molecular envelope of PCV2d virus-like particles identifies differences between PCV2a, b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.09.001

    authors： Khayat R,Wen K,Alimova A,Gavrilov B,Katz A,Galarza JM,Gottlieb P

    更新日期：2019-11-01 00:00:00

  • AFV1, a novel virus infecting hyperthermophilic archaea of the genus acidianus.

    abstract：:We describe a novel virus, AFV1, of the hyperthermophilic archaeal genus Acidianus. Filamentous virions are covered with a lipid envelope and contain at least five different proteins with molecular masses in the range of 23-130 kDa and a 20.8-kb-long linear double-stranded DNA. The virus has been assigned to the famil...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00481-1

    authors： Bettstetter M,Peng X,Garrett RA,Prangishvili D

    更新日期：2003-10-10 00:00:00

  • Changing sensitivity to broadly neutralizing antibodies b12, 2G12, 2F5, and 4E10 of primary subtype B human immunodeficiency virus type 1 variants in the natural course of infection.

    abstract：:The conserved nature of the epitopes of the four broadly neutralizing antibodies (BNAbs), b12, 2G12, 2F5, and 4E10, may imply that the sensitivity of HIV-1 for these BNAbs remains fairly constant over the course of infection. Here, we demonstrate that viruses isolated early during the course of infection were mostly s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.05.028

    authors： Bunnik EM,van Gils MJ,Lobbrecht MS,Pisas L,van Nuenen AC,Schuitemaker H

    更新日期：2009-08-01 00:00:00

  • Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus.

    abstract：:The lentivirus caprine arthritis-encephalitis virus (CAEV) is closely related by nucleotide sequence homology to visna virus and other sheep lentiviruses and shows less similarity to the other animal and human lentiviruses. The genomic organization of CAEV is very similar to that of visna virus and the South African o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90303-9

    authors： Saltarelli M,Querat G,Konings DA,Vigne R,Clements JE

    更新日期：1990-11-01 00:00:00

  • Unique physicochemical properties of human enteric Ad41 responsible for its survival and replication in the gastrointestinal tract.

    abstract：:Human enteric adenovirus Ad41 is associated with children gastroenteritis. To infect gastrointestinal cells, the invading virus must be acid-stable and resistant to inactivation by bile salts and proteases. In addition, it has to cross the mucus barrier before it infects mucosa cells. We show that Ad41 infectivity is ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.01.020

    authors： Favier AL,Burmeister WP,Chroboczek J

    更新日期：2004-04-25 00:00:00

  • Two adenovirus proteins with redundant activities in virus growth facilitates tripartite leader mRNA accumulation.

    abstract：:Most adenovirus-specific mRNAs expressed late after infection originate from a single transcription unit, the so-called major late transcription unit. All mRNAs expressed from this unit have in common a 201 nucleotide-long spliced tripartite leader segment attached to their 5' ends. Human adenovirus mutants that carry...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1234

    authors： Ohman K,Nordqvist K,Akusjärvi G

    更新日期：1993-05-01 00:00:00

  • Induction of herpes simplex virus gB-specific cytotoxic T lymphocytes in TAP1-deficient mice by genetic immunization but not HSV infection.

    abstract：:Loading of most endogenous peptides on major histocompatibility complex class I molecules is conditional on their transport into the endoplasmic reticulum (ER) by the peptide transporter TAP. We describe an HSV-2/1 cross-reactive cytotoxic T-cell (CTL) epitope that is processed in a TAP1-independent manner in vivo fol...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0829

    authors： Paliard X,Doe B,Selby MJ,Hartog K,Lee AY,Burke RL,Walker CM

    更新日期：2001-03-30 00:00:00

  • Identification and characterization of a protein kinase-interacting protein encoded by the Autographa californica nuclear polyhedrosis virus.

    abstract：:We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa pro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8944

    authors： Fan X,McLachlin JR,Weaver RF

    更新日期：1998-01-20 00:00:00

  • Polymorphisms of the cytidine deaminase APOBEC3F have different HIV-1 restriction efficiencies.

    abstract：:The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.11.004

    authors： Mohammadzadeh N,Follack TB,Love RP,Stewart K,Sanche S,Chelico L

    更新日期：2019-01-15 00:00:00

  • Zoonotic potential of highly pathogenic avian H7N3 influenza viruses from Pakistan.

    abstract：:H5 and H7 avian influenza viruses can become highly pathogenic in chickens after interspecies transmission. These viruses have transmitted directly to humans from birds in Eurasia and Africa (H5N1), the Netherlands (H7N7), and Canada (H7N3). Here we report antigenic, sequence, and phylogenetic analyses of H7N3 viruses...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.05.008

    authors： Aamir UB,Naeem K,Ahmed Z,Obert CA,Franks J,Krauss S,Seiler P,Webster RG

    更新日期：2009-08-01 00:00:00

  • Protein S and Gas6 induce efferocytosis of HIV-1-infected cells.

    abstract：:Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.12.025

    authors： Chua BA,Ngo JA,Situ K,Ramirez CM,Nakano H,Morizono K

    更新日期：2018-02-01 00:00:00

  • Difference in sensitivity to interferon among mouse hepatitis viruses with high and low virulence for mice.

    abstract：:Mouse hepatitis viruses (MHV) of different virulence for mice were studied with respect to interferon (IFN) sensitivity. The growth of low-virulent MHV-S and intermediately virulent MHV-JHM was significantly suppressed in IFN-treated L cells compared with untreated cells. However, a comparable suppression of the growt...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90225-9

    authors： Taguchi F,Siddell SG

    更新日期：1985-11-01 00:00:00

  • Lineage diversification of pigeon paramyxovirus effect re-emergence potential in chickens.

    abstract：:Genotype VI-paramyxovirus (GVI-PMV1) is a major cause of epidemic Newcastle-like disease in Columbiformes. This genotype of avian paramyxovirus type 1 has diversified rapidly since its introduction into the US in 1982 resulting in two extant lineages, which have different population growth properties. Although some GV...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.06.007

    authors： Chong YL,Kim O,Poss M

    更新日期：2014-08-01 00:00:00

  • Complementation of enteric adenovirus type 40 for lytic growth in tissue culture by E1B 55K function of adenovirus types 5 and 12.

    abstract：:The enteric adenovirus type 40 strain Dugan (Ad40) cannot be passaged in HeLa cells, but will grow in 293 cells, which express Ad5 E1 functions. To determine the reason for this limited host range, KB cell lines expressing Ad2 E1A, E1B, or E1A + E1B (L. E. Babiss, C. S. H. Young, P. B. Fisher, and H. S. Ginsberg, 1983...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90634-x

    authors： Mautner V,Mackay N,Steinthorsdottir V

    更新日期：1989-08-01 00:00:00

  • Production and characterization of monoclonal antibodies to porcine group C rotaviruses cross-reactive with group A rotaviruses.

    abstract：:Five monoclonal antibodies (MAbs) to porcine group (gp) C rotaviruses (Cowden and Ah strains) reactive with both gp A and C rotaviruses in cell culture immunofluorescence (CCIF) tests were produced and characterized. These MAbs reacted with three strains of gp A and two strains of gp C rotaviruses in a CCIF test and w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90189-v

    authors： Tsunemitsu H,Ojeh CK,Jiang B,Simkins RA,Weilnau PA,Saif LJ

    更新日期：1992-11-01 00:00:00

  • Comparison of human, simian, and bovine rotaviruses for requirement of sialic acid in hemagglutination and cell adsorption.

    abstract：:Human rotaviruses (Wa, KUN, MO) showed hemagglutination (HA) only with fixed 1-day-old chicken erythrocytes, and their HA activities were completely destroyed by trypsin activation of virions. Simian SA-11 and bovine NCDV had HA activities not only against fixed erythrocytes but also against fresh erythrocytes from va...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90121-9

    authors： Fukudome K,Yoshie O,Konno T

    更新日期：1989-09-01 00:00:00

  • Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin.

    abstract：:The binding of influenza virus to erythrocytes and host cells is mediated by the interaction of the viral hemagglutinin (H) with cell surface receptors containing sialic acid (SA). The specificity of this interaction for 19 human and animal influenza isolates was examined using human erythrocytes enzymatically modifie...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90150-2

    authors： Rogers GN,Paulson JC

    更新日期：1983-06-01 00:00:00

  • Specific cell surface requirements for the infection of CD4-positive cells by human immunodeficiency virus types 1 and 2 and by Simian immunodeficiency virus.

    abstract：:Human CD4 was expressed on a range of mammalian cell lines. CD4+ non-primate cells, derived from rat, hamster, mink, cat, and rabbit, bind recombinant gp120 of human immunodeficiency virus type 1 (HIV-1) but are resistant to HIV-1 infection. CD4 expression on various human, rhesus, and African green monkey cell lines ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90904-p

    authors： Clapham PR,Blanc D,Weiss RA

    更新日期：1991-04-01 00:00:00

  • CfMNPV blocks AcMNPV-induced apoptosis in a continuous midgut cell line.

    abstract：:Morphological and molecular changes produced by Autographa californica nuclear polyhedrosis virus (AcMNPV) infection in a permissive cell line, IPLB-SF-21AE (SF-21), of Spodoptera frugiperda and a nonpermissive cell line, FPMI-CF-203 (CF-203), of Choristoneura fumiferana are described. CF-203 cells inoculated with AcM...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0411

    authors： Palli SR,Caputo GF,Sohi SS,Brownwright AJ,Ladd TR,Cook BJ,Primavera M,Arif BM,Retnakaran A

    更新日期：1996-08-01 00:00:00

  • Construction and characterization of E1-minus replication-defective adenovirus vectors that express E3 proteins from the E1 region.

    abstract：:Previous research has indicated that the adenovirus protein complex named RID, derived from the E3 transcription unit, functions to remove the receptors named Fas/Apo1/CD95 (Fas) and epidermal growth factor receptor (EGFR) from the surface of cells. (The RID complex is composed of the RIDalpha and RIDbeta polypeptides...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1580

    authors： Toth K,Kuppuswamy M,Doronin K,Doronina O,Lichtenstein D,Tollefson A,Wold W

    更新日期：2002-09-15 00:00:00

  • The matrix protein of HIV-1 is not sufficient for assembly and release of virus-like particles.

    abstract：:The matrix (MA) proteins of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) are known to be important for the targeting and assembly of lentiviral proteins. The objective of the present study was to determine whether the MA protein of HIV-1 was sufficient for particle assembly and r...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9284

    authors： Giddings AM,Ritter GD Jr,Mulligan MJ

    更新日期：1998-08-15 00:00:00

  • In vitro synthesis of biologically active beet necrotic yellow vein virus RNA.

    abstract：:Beet necrotic yellow vein virus (BNYVV) has a quadripartite plus-strand RNA genome in which the two smallest genome components, RNA 3 and 4, are not necessary for virus multiplication in leaves. Infectious transcripts of BNYVV RNA 3 and 4 have already been described (V. Ziegler-Graff, S. Bouzoubaa, I. Jupin, H. Guille...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90131-1

    authors： Quillet L,Guilley H,Jonard G,Richards K

    更新日期：1989-09-01 00:00:00

  • Host cell p53 associates with the feline calicivirus major viral capsid protein VP1, the protease-polymerase NS6/7, and the double-stranded RNA playing a role in virus replication.

    abstract：:p53 is implicated in several cellular pathways such as induction of cell-cycle arrest, differentiation, senescence, and apoptosis. p53 is activated by a broad range of stress signals, including viral infections. While some viruses activate p53, others induce its inactivation, and occasionally p53 is differentially mod...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.08.008

    authors： Trujillo-Uscanga A,Gutiérrez-Escolano AL

    更新日期：2020-11-01 00:00:00

  • Adeno-associated virus infection of murine fibroblasts with help provided by mouse adenovirus.

    abstract：:Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant p...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.04.020

    authors： Bhrigu V,Trempe JP

    更新日期：2009-07-20 00:00:00

  • Circular DNA genomics (circomics) exemplified for geminiviruses in bean crops and weeds of northeastern Brazil.

    abstract：:Circomics was coined to describe the combination of rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) and pyro-sequencing to investigate the genome structures of small circular DNAs. A batch procedure is described using 61 plant samples from Asia, South America and Central America whi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.02.007

    authors： Wyant PS,Strohmeier S,Schäfer B,Krenz B,Assunção IP,Lima GS,Jeske H

    更新日期：2012-06-05 00:00:00

  • IL-12p40 gene-deficient BALB/c mice exhibit lower weight loss, reduced lung pathology and decreased sensitization to allergen in response to infection with pneumonia virus of mice.

    abstract：:Respiratory syncytial virus (RSV) is a major cause of bronchiolitis and pneumonia in infants and pneumonia virus of mice (PVM) causes similar disease. BALB/c mice are highly susceptible, while C57BL/6 mice are more resistant to PVM. IL-12 was significantly more up-regulated in response to PVM infection in BALB/c than ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.06.020

    authors： Shrivastava P,Sarkar I,Atanley E,Gomis S,van Drunen Littel-van den Hurk S

    更新日期：2016-10-01 00:00:00

  • Viral RNA load and histological changes in tissues following experimental infection with an arterivirus of possums (wobbly possum disease virus).

    abstract：:Tissues from Australian brushtail possums (Trichosurus vulpecula) that had been experimentally infected with wobbly possum disease (WPD) virus (WPDV) were examined to elucidate pathogenesis of WPDV infection. Mononuclear inflammatory cell infiltrates were present in livers, kidneys, salivary glands and brains of WPD-a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.07.003

    authors： Giles J,Perrott M,Roe W,Shrestha K,Aberdein D,Morel P,Dunowska M

    更新日期：2018-09-01 00:00:00

  • Tumorigenicity of adenovirus-transformed cells: region E1A of adenovirus 12 confers resistance to natural killer cells.

    abstract：:Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12E1AB virus (containing E1A and E1B...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90143-6

    authors： Sawada Y,Föhring B,Shenk TE,Raska K Jr

    更新日期：1985-12-01 00:00:00

  • High throughput sequence analysis reveals hitherto unreported recombination in the genus Norovirus.

    abstract：:Viruses of the Norovirus genus (Caliciviridae family) are a major cause of human gastroenteritis. In some viruses, recombination is an important evolutionary process and therefore we should try to discover the quantity and characteristics of such events in Noroviruses. In order to identify recombination events, multip...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.09.051

    authors： Etherington GJ,Dicks J,Roberts IN

    更新日期：2006-02-05 00:00:00

  • MHV-A59 enters polarized murine epithelial cells through the apical surface but is released basolaterally.

    abstract：:Coronaviruses have a marked tropism for epithelial cells. Entry and release of the porcine transmissible gastroenteritis virus (TGEV) is restricted to apical surfaces of polarized epithelial cells, as we have recently shown (J. W. A. Rossen, C. P. J. Bekker, W. F. Voorhout, G. J. A. M. Strous, A. van der Ende, and P. ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1316

    authors： Rossen JW,Voorhout WF,Horzinek MC,van der Ende A,Strous GJ,Rottier PJ

    更新日期：1995-06-20 00:00:00