Abstract:
:Human adenovirus (HAdV) is a cause of significant morbidity and mortality in immunocompromised patients, especially after stem cell transplantation (SCT). Viral clearance has been attributed to CD4(+) T-cell responses against the Hexon-protein, but the frequency of specific T(HELPER) cells is extremely low or not detectable ex vivo and preference for different CD4(+) T-cell epitopes is variable among individuals. We therefore analyzed 44 healthy donors and 6 SCT-recipients for Hexon-specific CD4(+)-responses ex vivo, to identify epitopes which would be broadly applicable. We selected 19 candidate epitopes with predicted restriction to HLA-DR1/DR3/DR4/DR7; 16 were located within the highly conserved regions, indicating cross-reactivity of T cells among HAdV-subspecies. Ten epitopes induced CD4(+)-proliferation in >50% of individuals, confirmed by intracellular IFN-gamma detection. Three SCT recipients who recovered from an infection with HAdV displayed reactivity towards only a single hexon epitope, whereas healthy individuals were responsive to two to eight epitopes (median 3). The ex vivo detection of Hexon-specific CD4(+) T-cells, without any long-term culture in vitro, enables the detection and generation of HAdV-specific CD4(+) T cells for adoptive T-cell transfer against HAdV-infection post SCT.
journal_name
Virologyjournal_title
Virologyauthors
Serangeli C,Bicanic O,Scheible MH,Wernet D,Lang P,Rammensee HG,Stevanovic S,Handgretinger R,Feuchtinger Tdoi
10.1016/j.virol.2009.10.049subject
Has Abstractpub_date
2010-02-20 00:00:00pages
277-84issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(09)00696-5journal_volume
397pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Epstein-Barr virus (EBV) is widely spread in the human population. EBV nuclear antigen 1 (EBNA1) is a transcription factor that activates viral genes and is necessary for viral replication and partitioning, which binds the EBV genome cooperatively. We identify similar EBNA1 repeat binding sites in the human genome usi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.040
更新日期:2010-09-30 00:00:00
abstract::Phage-display methodology has been used to select variant CD4 proteins exhibiting increased binding to the surface envelope glycoprotein, gp120, of Human Immunodeficiency Virus Type-1. To facilitate the selection, a library of mutant CD4 proteins was constructed by cloning a PCR-generated error prone population of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8651
更新日期:1997-08-04 00:00:00
abstract::Differing reports exist about the replication of human enteric adenoviruses (EnAds) in various cell lines. There was a suggestion that EnAds are defective, do not grow on primary human diploid cells, and behave like Ad host-range mutants, i.e., they require early gene products from other Ad types for efficient growth....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90072-y
更新日期:1990-01-01 00:00:00
abstract::We report the complete nucleotide sequence of the bovine herpesvirus 1 (BHV-1) immediate-early gene encoding BICP4, the homolog of the ICP4 protein of herpes simplex virus. Combined with previous mapping studies, the sequence analysis revealed that the transcript for BICP4 consisted of a noncoding leader RNA (exon 1; ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1596
更新日期:1993-11-01 00:00:00
abstract::Animal viruses profoundly modify the metabolism of lipids, the synthesis of new membranes, and membrane traffic. These alterations are related to the replication of viral genomes. Addition of oleic acid from the beginning of poliovirus infection inhibits the appearance of virus polypeptides at concentrations that do n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90802-i
更新日期:1991-11-01 00:00:00
abstract::Infection of different human epithelial cell lines with human respiratory syncytial virus (HRSV) revealed significant differences in the electrophoretic mobility of the viral attachment glycoprotein (G). Cell-type specific differences in G protein glycosylation were observed with certain lectins and sugar-specific rea...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0379
更新日期:1996-07-15 00:00:00
abstract::Attempts to define the genetic determinants required for efficient growth of human immunodeficiency virus type 1 (HIV-1) in monocyte-macrophages were made by constructing chimeras between two infectious clones of HIV-1 (HXB2 and LW/C), which despite only minor differences in their DNA sequence have striking difference...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80082-4
更新日期:1995-01-10 00:00:00
abstract::A collection of TK+, ACV-resistant mutants of herpes simplex virus type 1 (HSV-1) has been derived using a selection system based on biochemically transformed cells. Evidence is presented suggesting that most of these mutants induce resistant DNA polymerase activities and are thus likely to express variant DNA polymer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90009-1
更新日期:1985-10-30 00:00:00
abstract::African swine fever virus (ASFV) growth and plaque formation were inhibited by phosphonoacetic acid (PAA) concentrations of 200 micrograms/ml or more. One spontaneous mutant and two mutants isolated from mutagenized virus were resistant to PAA inhibition and showed practically normal viral DNA synthesis in the presenc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.9953
更新日期:1995-12-01 00:00:00
abstract::During respiratory syncytial virus (RSV) infection there is a close physical interaction between the filamentous actin (F-actin) and the virus, involving both inclusion bodies and the virus filaments. This interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.007
更新日期:2007-12-20 00:00:00
abstract::Rotavirus infection was studied in adult nude mice (BALB/c background), alpha beta or gamma delta and alpha beta/gamma delta T cell receptor (TCR) knockout (-/-) mice (C57BL/6 and C57BL/6 x 129 backgrounds), and SCID mice (C57BL/6 background). The gamma delta TCR -/- mice cleared infection just like control mice. All ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8843
更新日期:1997-11-24 00:00:00
abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf11 (bm11) is a highly conserved gene with unknown function. It is homologous to AcMNPV orf19. In this study, a bm11 knockout virus was constructed and its role was investigated. Expression analysis indicated that bm11 is a late gene and confocal microscopy analysis demonstra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.10.026
更新日期:2019-01-15 00:00:00
abstract::Little is known about the molecular determinants causing and sustaining viral persistent infections at the cellular level. We found that Drosophila cells persistently infected (PI) with Flock House virus (FHV) invariably harbor defective viral RNAs, which are replicated by the FHV RNA-dependent RNA polymerase. Some de...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.08.002
更新日期:2011-10-10 00:00:00
abstract::A major neutralizing epitope (here referred to as the T332 epitope) located on the lateral surface of domain III (DIII) of the West Nile virus (WNV) envelope protein has been identified based on the analysis of murine monoclonal antibodies. However, little is known about the humoral immune response against WNV in a na...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.047
更新日期:2007-03-15 00:00:00
abstract::Cellular organelles of cauliflower mosaic virus-infected turnip plants were isolated, incubated with 32P-labeled deoxyribonucleoside triphosphates and assayed for their ability to synthesize virus-specific DNA. Only nuclei were found to synthesize a labeled DNA product. This DNA migrated during agarose gel electrophor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90124-6
更新日期:1982-08-01 00:00:00
abstract::The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90140-0
更新日期:1985-12-01 00:00:00
abstract::A crucial step in the arenavirus life cycle is the proteolytic processing of the viral envelope glycoprotein precursor (GPC) by the cellular proprotein convertase (PC) subtilisin kexin isozyme-1 (SKI-1)/site-1 protease (S1P). Here we conducted a systematic and quantitative analysis of SKI-1/S1P processing of peptides ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.04.021
更新日期:2011-08-15 00:00:00
abstract::Previously, an epitope recognized by a set of neutralizing monoclonal antibodies directed against the S protein of transmissible gastroenteritis has been identified. This neutralization epitope can be simulated by a single peptide combining residues 380 to 387 and 1176 to 1184 of the S protein; this combination peptid...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90684-4
更新日期:1991-05-01 00:00:00
abstract::We have constructed chimeric papillomavirus-like particles (CVLPs) by replacing the 34-carboxy-terminal amino acids of the HPV 16 L1 protein with various parts of the HPV 16 E7 protein. Chimeric proteins were expressed by recombinant baculoviruses and analyzed by electron microscopy for their ability to assemble into ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8591
更新日期:1997-07-21 00:00:00
abstract::The complete sequence of the genome of the cherry strain of tomato bushy stunt virus (TBSV), a member of the tombusvirus group, was determined. A full-length clone of the genome containing a bacteriophage T7 RNA polymerase promoter was assembled from partial cDNA clones. In vitro transcripts of the genome, either with...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90468-7
更新日期:1990-07-01 00:00:00
abstract::Alphaviruses are mosquito-borne viruses that cause serious human and animal diseases. Previous studies demonstrated that a determinant within the nsP1/nsP2 cleavage domain of the virulent Sindbis AR86 virus played a key role in regulating adult mouse virulence without adversely affecting viral replication. Additional ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.031
更新日期:2010-03-30 00:00:00
abstract::Measles virus (MV) inhibits lymphocyte function in patients, as well as in cells infected in vitro. The proliferation of phytohemagglutinin-stimulated T lymphocytes is suppressed by in vitro MV infection, as shown by the diminished incorporation of [3H]thymidine into DNA and the reduced frequency of cells in the S pha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90316-h
更新日期:1992-03-01 00:00:00
abstract::The porcine lymphotropic herpesvirus 1 (PLHV-1), the first gammaherpesvirus of pigs, has been detected at a high prevalence in healthy pig populations. A porcine gammaherpesvirus has also been detected at high copy numbers in animals suffering from posttransplant lymphoproliferative disease (PTLD). While human PTLD is...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1390
更新日期:2002-03-15 00:00:00
abstract::Human enteric adenovirus Ad41 is associated with children gastroenteritis. To infect gastrointestinal cells, the invading virus must be acid-stable and resistant to inactivation by bile salts and proteases. In addition, it has to cross the mucus barrier before it infects mucosa cells. We show that Ad41 infectivity is ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.01.020
更新日期:2004-04-25 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) glycoprotein B (gB) is a multifunctional glycoprotein required for infectivity; it is thought to promote fusion of the viral envelope with the cell membrane and entry of virions into cells. To map the antigenic and functional domains on gB, we constructed amino terminal derivatives lacki...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90102-5
更新日期:1989-09-01 00:00:00
abstract::Herpes simplex virus 1 glycoprotein B (gB) is one of 10 glycoproteins in the virion envelope and in the membranes of infected cells. It is required for infection of cells in culture and functions in penetration of the cell by fusing the virion envelope with the plasma membrane. In studies to map the functional domains...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90064-v
更新日期:1992-01-01 00:00:00
abstract::A viroid has been purified from avocado leaves infected by sunblotch disease and designated the avocado sunblotch viroid. It is a covalently closed circular RNA molecule with a molecular weight lower than that of chrysanthemum stunt viroid and citrus exocortis viroid while hybridization analysis with 32P-labeled compl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90045-x
更新日期:1979-11-01 00:00:00
abstract::Virus-host interactions essential for alphavirus pathogenesis are poorly understood. To address this shortcoming, we coupled retrovirus insertional mutagenesis and a cell survival selection strategy to generate clonal cell lines broadly resistant to Sindbis virus (SINV) and other alphaviruses. Resistant cells had sign...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.025
更新日期:2008-07-20 00:00:00
abstract::Infection of Salmonella enterica with lytic mutants of either P22 or SE1 bacteriophages triggers the expression of its DNA damage-inducible SOS response through a lexA-dependent pathway. This induction of the SOS system strictly requires the presence of the bacteriophage kil gene. Accordingly, plasmid overexpression o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.001
更新日期:2006-08-01 00:00:00
abstract::Primate lentiviruses are composed of several distinct lineages, including human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus SIVagm. HIV-1 and HIV-2 have significant differences in the mechanisms of viral RNA encapsidation. Therefore, the RNA packaging mechanisms of SIVagm cannot be ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.030
更新日期:2007-06-20 00:00:00