Abstract:
:Circomics was coined to describe the combination of rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) and pyro-sequencing to investigate the genome structures of small circular DNAs. A batch procedure is described using 61 plant samples from Asia, South America and Central America which revealed 83 contig sequences of geminiviral DNA components and 4 contig sequences of DNA satellites. The usefulness of this approach is validated for the Brazilian begomoviruses, and the sequence fidelity is determined by comparing the results with those of conventional cloning and sequencing of Bolivian begomoviruses reported recently. Therefore, circomics has been proven to be a major step forward to economize costs and labor and to characterize reliably geminiviral genomes in their population structure of the quasispecies.
journal_name
Virologyjournal_title
Virologyauthors
Wyant PS,Strohmeier S,Schäfer B,Krenz B,Assunção IP,Lima GS,Jeske Hdoi
10.1016/j.virol.2012.02.007subject
Has Abstractpub_date
2012-06-05 00:00:00pages
151-7issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(12)00102-Xjournal_volume
427pub_type
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