Abstract:
:Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing efficient foreign gene expression in B-LCL using biotinylated adenovirus as an endosome-disrupting agent. Plasmid DNA is coupled to the exterior of viral particles by streptavidin-polylysine chimeric proteins. Up to 67% of B-LCL may be induced to express foreign genes in vitro in transient expression systems, and gene expression lasts for at least 17 days. We have expressed firefly luciferase, beta-galactosidase (beta-gal), chloramphenicol acetyltransferase, HIV gag, and env genes, as well as infectious HIV, and the EBV-specific BZLF gene in B-LCL with this system. In vivo delivery of a beta-gal reporter gene to B-LCL was documented in a SCID mouse model. Potential applications include study of genetic regulation of EBV infection and transformation events, study of potential gene therapies for EBV-related B-cell tumors, and production of antigen-presenting cells for use in immunologic assays. Because of the high percentage of cells transformed and the length of foreign gene expression, the possibility of examining foreign gene expression in transient assays, without selection for clonal populations, exists.
journal_name
Virologyjournal_title
Virologyauthors
Curiel TJ,Cook DR,Bogedain C,Jilg W,Harrison GS,Cotten M,Curiel DT,Wagner Edoi
10.1006/viro.1994.1069subject
Has Abstractpub_date
1994-02-01 00:00:00pages
577-85issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71069-5journal_volume
198pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The enveloped virions of two strains of a granulosis virus, a synergistic Hawaiian (GVH) and a nonsynergistic Oregonian (GVO) strain which infect the armyworm, Pseudaletia unipuncta, were liberated from their capsules with 0.02 N NaOH and purified by filtration through membrane filters. The envelopes were solubilized ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90439-2
更新日期:1979-04-15 00:00:00
abstract::Alphaviruses are mosquito-borne viruses that cause serious human and animal diseases. Previous studies demonstrated that a determinant within the nsP1/nsP2 cleavage domain of the virulent Sindbis AR86 virus played a key role in regulating adult mouse virulence without adversely affecting viral replication. Additional ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.031
更新日期:2010-03-30 00:00:00
abstract::The role of the matrix (MA) domain of simian immunodeficiency virus (SIV) and bovine leukaemia virus (BLV) Gag in the assembly of virus-like particles (VLP) in insect cells has been investigated. Wild-type SIV and BLV Gag assembled to form discrete VLP structures typical of many retroviruses analysed by similar system...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1441
更新日期:2002-07-20 00:00:00
abstract::An infectious and full-length molecular clone of genomic human spumaretrovirus (HSRV) DNA was constructed. The infectivity of the pHSRV13 clone was demonstrated after transfection into susceptible cells by passage of HSRV-specific cytopathic effects as a cell-free culture supernatant, by electron microscopy of HSRV pa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90820-2
更新日期:1991-09-01 00:00:00
abstract::The genetic structure of the population of Watermelon mosaic virus (WMV) in Spain was analysed by the biological and molecular characterisation of isolates sampled from its main host plant, melon. The population was a highly homogeneous one, built of a single pathotype, and comprising isolates closely related genetica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.10.002
更新日期:2004-01-05 00:00:00
abstract::The phosphorylated protein pp65 of human Cytomegalovirus (HCMV) is the predominant virion protein and the major tegument constituent. It plays important roles in HCMV infection and virion assembly. Live cell imaging and fluorescence recovery after photobleaching (FRAP) analysis showed that HCMV pp65 accumulated dynami...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.021
更新日期:2009-09-30 00:00:00
abstract::Expression of alphavirus glycoproteins on the surface of infected cells leads to cell fusion after exposure to acidic pH. Two strains of Sindbis virus, AR339 (SV) and neuroadapted Sindbis virus (NSV), which differ in virulence for weanling mice, were found to differ in pH-dependent fusion. BHK-21 cells infected with S...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90178-5
更新日期:1989-04-01 00:00:00
abstract::Hepatitis delta antigen (HDAg) of hepatitis delta virus (HDV) typically consists of two related protein species. The small HDAg (S-HDAg) is a 24-kDa protein of 195 amino acids and the large HDAg (L-HDAg) is a 27-kDa protein with an additional 19 amino acids at its C-terminus. These two proteins have distinct functions...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0680
更新日期:2000-12-20 00:00:00
abstract::Activation of the minute virus of mice (MVM) P4 promoter is a key step in the life cycle of the virus and is completely dependent on host transcription factors. Since transcription-factor composition varies widely in different cell types, there is the possibility that only some cell types in the host organism have the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00020-x
更新日期:2003-02-15 00:00:00
abstract::We compared the outcome of different cellular and viral factors on the regulation of the HPV-16 early viral gene expression in trophoblastic and cervical cancer cells. A high variability of the long control (LCR) activity was observed, prompting us to evaluate the role of secreted factors in the control of the early g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.12.056
更新日期:2011-03-30 00:00:00
abstract::Gammaherpesviruses establish life-long infection in most adults and are associated with the development of B cell lymphomas. While the interaction between gammaherpesviruses and splenic B cells has been explored, very little is known about gammaherpesvirus infection of B-1 B cells, innate-like B cells that primarily r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.02.022
更新日期:2016-05-01 00:00:00
abstract::The Gardner (GA) and Snyder-Theilen (ST) isolates of feline sarcoma virus (FeSV) represent genetic recombinants between feline leukemia virus (FeLV) and transformation-specific sequences (v-fes gene) of cat cellular origin. A related transforming gene (v-fps), common to the Fujinami, PRC II, and UR 1 strains of avian ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90219-2
更新日期:1983-03-01 00:00:00
abstract::West Nile virus (WNV) is a flavivirus that swept rapidly across North America in 1999, declined in prevalence, and then resurged in 2012. To date, no vaccine is available to prevent infection in the human population. Herpes simplex virus (HSV) replication-defective vaccine vectors induce a durable immunity characteriz...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.006
更新日期:2016-09-01 00:00:00
abstract::Terminally redundant linear proviral DNA of approximately 9.5 kb was the major unintegrated species recovered in the Hirt supernatant fraction of caprine synovial membrane cells infected with strain 75-G63 caprine arthritis-encephalitis virus. A physical map based on the cleavage sites of 13 restriction endonucleases ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90318-0
更新日期:1984-04-30 00:00:00
abstract::Processing of the transmembrane glycoprotein (GP) of Marburg virus involved the conversion of an endo H-sensitive, ER-specific form into an endo H-resistant, Golgi-specific precursor that was cleaved into GP(1) and GP(2). Cleavage was mediated by furin or another subtilisin-like endoprotease with similar substrate spe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0110
更新日期:2000-03-01 00:00:00
abstract::The monoclonal antibody 2D5 neutralized vaccinia virus by preventing penetration of the virus and reacting with VP23-29K. The conformation of the VP23-29K was maintained by a disulfide bond(s), and the 2D5mAb reacted stronger with the nonreduced 23-kDa form than with the reduced 29-kDa form. We selected several escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0337
更新日期:1996-06-15 00:00:00
abstract::Infection with a broad diversity of viruses often activates host cell signaling pathways including the mitogen-activated protein kinase pathway. The present study established that dengue virus infection of human macrophages activates Jun NH(2)-terminal kinase (JNK) and the p38 MAPKs pathways. The activation was observ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.019
更新日期:2010-01-05 00:00:00
abstract::We reported previously that carbohydrate attachment to an overlapping glycosylation site adjacent to the signal peptide of GP3 from equine arteritis virus (EAV) prevents cleavage. Here we investigated whether this unusual processing scheme is a feature of GP3s of other Arteriviridae, which all contain a glycosylation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.11.026
更新日期:2018-04-01 00:00:00
abstract::Several VV structural proteins are produced by the removal of amino-terminal peptides from their cognate precursors. In the experiments reported here, directed genetic approaches were used to investigate the possible role of these terminal peptides in protein processing. As a model system, the FLAG epitope-tagged P25K...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1069
更新日期:1995-02-20 00:00:00
abstract::Human immunodeficiency virus type 1 subtype C (HIV-1C) accounts for about 50% of all HIV infections in the pandemic and is the predominant subtype in the heavily burdened region of southern Africa. HIV-1C possesses unique genetic and phenotypic features that might be associated with biological differences compared to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.047
更新日期:2006-04-10 00:00:00
abstract::Classical swine fever virus (CSFV) is a positive-sense RNA virus with a high degree of genetic variability among isolates. High diversity is also found in virulence, with strains covering the complete spectrum from avirulent to highly virulent. The underlying genetic determinants are far from being understood. Since R...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.020
更新日期:2013-03-30 00:00:00
abstract::Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain E...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.03.013
更新日期:2017-06-01 00:00:00
abstract::During head assembly of phage T3, DNA is packaged into the cavity of a preformed protein shell, called the prohead, with the aid of noncapsid, packaging proteins, the products of genes 18 and 19 (gp18 and gp19). gp18 and gp19 separately form complexes with DNA and proheads, respectively. These complexes associate to f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90550-u
更新日期:1991-12-01 00:00:00
abstract::Two HLA-A*02-restricted epitopes have been identified within the VP1 polypeptide of a human polyomavirus, BK virus, which is associated with polyomavirus-associated nephropathy in kidney transplant patients. Immunization of transgenic mice with recombinant modified vaccinia Ankara expressing BKV VP1 (rMVA-BKV VP1) eli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.02.040
更新日期:2006-06-20 00:00:00
abstract::We produced the putative protease 3C of human rhinovirus 1A (HRV-1A), a minor group rhinovirus, by Escherichia coli expression of a segment of HRV-1A cDNA coding for 3A, 3B, 3C, and parts of 2C and 3D (delta 2C3ABC delta 3D). The protease 3C was expected to be processed by intramolecular Q-G cleavages from the virus-s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90429-f
更新日期:1991-10-01 00:00:00
abstract::To better understand the role of specific residues within the duck hepatitis B virus (DHBV) pre-S protein in neutralization and infectivity, we have selected and identified pre-S variants which escape neutralization. A highly neutralizing monoclonal antibody (Mab 900) which recognizes an epitope 83IPQPQWTP90 localized...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8665
更新日期:1997-08-04 00:00:00
abstract::Syncytia formation has been considered important for cell-to-cell spread and pathogenesis of many viruses. As a syncytium forms, individual nuclei often congregate together, allowing close contact of nuclear membranes and possibly fusion to occur. However, there is currently no reported evidence of nuclear membrane fu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.09.002
更新日期:2017-12-01 00:00:00
abstract::Immunoelectron microscopy using an anti-VP2 monoclonal antibody complexed to colloidal gold has been used to study the mechanism of bluetongue virus (BTV) release from infected cells. Examination of the BTV-infected cell surface revealed that viruses are released both as enveloped particles, by budding through the pla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90218-3
更新日期:1989-11-01 00:00:00
abstract::Altered cytokine profiles and imbalanced frequencies of CD4(+) T helper cell subsets are thought to be linked with HIV-1/AIDS pathogenesis, but the causes need to be further clarified. Histamine, a biogenic amine with many functions, shows enhancement in HIV-1 infected individuals, which are considered to link with di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.04.009
更新日期:2013-08-01 00:00:00
abstract::Potato spindle tuber viroid (PSTVd) is a small non-protein-coding RNA pathogen that can induce disease symptoms in a variety of plant species. How PSTVd induces disease symptoms is a long standing question. It has been suggested that PSTVd-derived small RNAs (sRNAs) could direct RNA silencing of a targeted host gene(s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.12.019
更新日期:2014-02-01 00:00:00