Abstract:
:West Nile virus (WNV) is a flavivirus that swept rapidly across North America in 1999, declined in prevalence, and then resurged in 2012. To date, no vaccine is available to prevent infection in the human population. Herpes simplex virus (HSV) replication-defective vaccine vectors induce a durable immunity characterized by strong antibody and CD8(+) T cell responses even in HSV-immune animals. In this study, a WNV protein expression cassette was optimized for virus-like particle (VLP) production in transfection studies, and the cassette was recombined into an HSV-1 d106-WNV virus vector, which produced extracellular VLPs, as confirmed by immunoelectron microscopy. Immunization of mice with the d106-WNV recombinant vector elicited a specific anti-WNV IgG response. This study highlights the flavivirus coding sequences needed for efficient assembly of virus-like particles. This information will facilitate generation of additional vaccine vectors against other flaviviruses including the recently emerged Zika virus.
journal_name
Virologyjournal_title
Virologyauthors
Taylor TJ,Diaz F,Colgrove RC,Bernard KA,DeLuca NA,Whelan SPJ,Knipe DMdoi
10.1016/j.virol.2016.06.006subject
Has Abstractpub_date
2016-09-01 00:00:00pages
186-193eissn
0042-6822issn
1096-0341pii
S0042-6822(16)30147-7journal_volume
496pub_type
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