Outer nuclear membrane fusion of adjacent nuclei in varicella-zoster virus-induced syncytia.


:Syncytia formation has been considered important for cell-to-cell spread and pathogenesis of many viruses. As a syncytium forms, individual nuclei often congregate together, allowing close contact of nuclear membranes and possibly fusion to occur. However, there is currently no reported evidence of nuclear membrane fusion between adjacent nuclei in wild-type virus-induced syncytia. Varicella-zoster virus (VZV) is one typical syncytia-inducing virus that causes chickenpox and shingles in humans. Here, we report, for the first time, an interesting observation of apparent fusion of the outer nuclear membranes from juxtaposed nuclei that comprise VZV syncytia both in ARPE-19 human epithelial cells in vitro and in human skin xenografts in the SCID-hu mouse model in vivo. This work reveals a novel aspect of VZV-related cytopathic effect in the context of multinucleated syncytia. Additionally, the information provided by this study could be helpful for future studies on interactions of viruses with host cell nuclei.






Wang W,Yang L,Huang X,Fu W,Pan D,Cai L,Ye J,Liu J,Xia N,Cheng T,Zhu H




Has Abstract


2017-12-01 00:00:00














  • Expression of authentic vaccinia virus-specific and inserted viral and cellular genes under control of an early vaccinia virus promoter is regulated post-transcriptionally in interferon-treated chick embryo fibroblasts.

    abstract::The interferon sensitivity of the expression of an influenza-virus hemagglutinin (HA) gene cloned into the thymidine kinase (TK) gene of vaccinia virus was studied in chick embryo fibroblasts (CEF) and Madin-Darby bovine kidney (MDBK) cells. In CEF, the expression of the HA gene is inhibited by pretreatment of cells w...


    pub_type: 杂志文章


    authors: Degen HJ,Blum D,Grün J,Jungwirth C

    更新日期:1992-05-01 00:00:00

  • Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.

    abstract::We have recently described the cloning of a portion of the hepatitis E virus (HEV) and confirmed its etiologic association with enterically transmitted (waterborne, epidemic) non-A, non-B hepatitis. The virus consists of a single-stranded, positive-sense RNA genome of approximately 7.5 kb, with a polyadenylated 3' end...


    pub_type: 杂志文章


    authors: Tam AW,Smith MM,Guerra ME,Huang CC,Bradley DW,Fry KE,Reyes GR

    更新日期:1991-11-01 00:00:00

  • Helper virus-dependent replication, nucleotide sequence and genome organization of the satellite virus of maize white line mosaic virus.

    abstract::Virus like particles (17 nm in diameter) associated with maize white line mosaic virus (MWLMV) were shown to be a satellite virus of MWLMV (SV-MWLMV) on the basis of the following properties: (1) The SV-MWLMV was dependent upon the presence of MWLMV for replication in maize, while the latter virus could replicate inde...


    pub_type: 杂志文章


    authors: Zhang L,Zitter TA,Palukaitis P

    更新日期:1991-02-01 00:00:00

  • Bovine aortic endothelial cells are susceptible to hantavirus infection; a new aspect in hantavirus ecology.

    abstract::Hantaviruses are enveloped RNA viruses that belong to the family Bunyaviridae. They are the causative agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Hantaviruses show a worldwide distribution with specific rodent species as natural hosts. It is known that rodents can tr...


    pub_type: 杂志文章


    authors: Muranyi W,Kehm R,Bahr U,Müller S,Handermann M,Darai G,Zeier M

    更新日期:2004-01-05 00:00:00

  • Delineation of a 16 amino acid sequence that forms a core DNA recognition motif in the Epstein-Barr virus EBNA-1 protein.

    abstract::EBNA-1 is the sole virally specified protein required for replication of the Epstein-Barr virus latency origin and also modulates its own expression. Both of these functions are mediated through specific DNA binding. We created a series of amino acid substitutions across the previously identified DNA-binding domain of...


    pub_type: 杂志文章


    authors: Chen MR,Zong J,Hayward SD

    更新日期:1994-12-01 00:00:00

  • The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells.

    abstract::The expression and subcellular location of the 33.6-kDa nonstructural protein NSm of tomato spotted wilt virus (TSWV) was analyzed in Nicotiana rustica plants and protoplasts as a function of time. Immunofluorescent studies in protoplasts isolated from TSWV-infected N. rustica leaves showed that this protein could fir...


    pub_type: 杂志文章


    authors: Storms MM,Kormelink R,Peters D,Van Lent JW,Goldbach RW

    更新日期:1995-12-20 00:00:00

  • Deoxyribonucleoprotein complexes and DNA synthesis of herpes simplex virus type 1.

    abstract::Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...


    pub_type: 杂志文章


    authors: Leinbach SS,Casto JF,Pickett TK

    更新日期:1984-09-01 00:00:00

  • Residues in the PB2 and PA genes contribute to the pathogenicity of avian H7N3 influenza A virus in DBA/2 mice.

    abstract::Replication and transmission of avian influenza virus in humans poses a pandemic threat. The molecular determinants that facilitate this process are not well understood. We used DBA/2 mice to identify viral factors that mediate the difference in pathogenesis between a virulent (H7N3) and a non-virulent (H7N9) avian in...


    pub_type: 杂志文章


    authors: DesRochers BL,Chen RE,Gounder AP,Pinto AK,Bricker T,Linton CN,Rogers CD,Williams GD,Webby RJ,Boon AC

    更新日期:2016-07-01 00:00:00

  • Analysis of the SH3-binding region of HIV-1 nef: partial functional defects introduced by mutations in the polyproline helix and the hydrophobic pocket.

    abstract::An SH3-binding domain within the Nef protein of primate lentiviruses has been reported to be important to viral replication and infectivity and dispensable for CD4 downregulation, but its precise role remains unclear. This study investigates the effects of mutations in both the polyproline helix and in the hydrophobic...


    pub_type: 杂志文章


    authors: Craig HM,Pandori MW,Riggs NL,Richman DD,Guatelli JC

    更新日期:1999-09-15 00:00:00

  • Glycophorin is the reovirus receptor on human erythrocytes.

    abstract::Purified glycophorin (predominantly type A) from human erythrocytes was found to effectively inhibit reovirus hemagglutination (HA) in contrast to other glycoproteins such as fetuin or ovalbumin. Glycophorin was also a potent inhibitor of reovirus and protein sigma 1 binding to mouse L fibroblasts. Glycophorin pretrea...


    pub_type: 杂志文章


    authors: Paul RW,Lee PW

    更新日期:1987-07-01 00:00:00

  • Synthesis and subcellular localization of the murine coronavirus nucleocapsid protein.

    abstract::The synthesis and processing of the nucleocapsid protein (pp60) of the JHM strain of murine coronaviruses were examined. Pulse-chase experiments showed that pp60 was synthesized initially as a protein of approximately 57,000 in molecular weight (p57). Immunoprecipitation using mouse anti-JHMV antiserum indicated that ...


    pub_type: 杂志文章


    authors: Stohlman SA,Fleming JO,Patton CD,Lai MM

    更新日期:1983-10-30 00:00:00

  • Changes in locations of crossover sites over time in de novo generated RNA recombinants.

    abstract::Recombinant RNAs generated in plants 3 weeks postinoculation with turnip crinkle virus (TCV) genomic RNA and an associated satellite RNA, sat-RNA D, have a majority of TCV crossover sites in a 24-nucleotide repeat (motif IIIA/IIIB) that forms part of a stable hairpin (Carpenter et al., 1995, J. Mol.Biol. 245, 608-622)...


    pub_type: 杂志文章


    authors: Carpenter CD,Simon AE

    更新日期:1996-09-01 00:00:00

  • Replication of hepatitis B virus in a hepatocellular carcinoma.

    abstract::Hepatitis B virus transcripts and DNA from paired samples of neoplastic and nonneoplastic liver tissue of HBsAg seropositive patients were analyzed. The data obtained support the view that transcription of integrated DNA is frequent, both in neoplastic as well as in nonneoplastic liver tissue. In the case of one patie...


    pub_type: 杂志文章


    authors: Loncarević IF,Schranz P,Zentgraf H,Liang XH,Herrmann G,Tang ZY,Schröder CH

    更新日期:1990-01-01 00:00:00

  • Restriction of a Sindbis virus mutant in BHK cells and relief of the restriction by the addition of adenosine.

    abstract::SV(PZF) is a mutant of Sindbis virus (SV) which we selected on the basis of its ability to replicate in mosquito cells treated with pyrazofurin (PZF), a drug which inhibits pyrimidine nucleotide biosynthesis (Lin et al., 2000, Virology 272, 61-71). Three mutations, A6627U, A7543U, and C7593A, were identified in the ns...


    pub_type: 杂志文章


    authors: Lin YH,Simmonds HA,Stollar V

    更新日期:2002-01-05 00:00:00

  • Construction of recombinant avian infectious laryngotracheitis virus expressing the beta-galactosidase gene and DNA sequencing of the insertion region.

    abstract::Avian infectious laryngotracheitis virus (ILTV), a herpesvirus, is a highly contagious pathogen that causes an upper respiratory tract infection in chickens. It is one of the major problems in the poultry industry worldwide. Current vaccines are not satisfactory due to the induction of latent infection. Here we descri...


    pub_type: 杂志文章


    authors: Guo P,Scholz E,Maloney B,Welniak E

    更新日期:1994-08-01 00:00:00

  • Initial characterization of vaccinia virus B4 suggests a role in virus spread.

    abstract::Currently, little is known about the ankyrin/F-box protein B4. Here, we report that B4R-null viruses exhibited reduced plaque size in tissue culture, and decreased ability to spread, as assessed by multiple-step growth analysis. Electron microscopy indicated that B4R-null viruses still formed mature and extracellular ...


    pub_type: 杂志文章


    authors: Burles K,Irwin CR,Burton RL,Schriewer J,Evans DH,Buller RM,Barry M

    更新日期:2014-05-01 00:00:00

  • Biochemical studies of the maturation of herpesvirus nucleocapsid species.

    abstract::Three distinct species of nucleocapsids of equine herpesvirus type-1, designated as either light (L), intermediate (I), or heavy (H) on the basis of their densities in Renografin-76 density gradients (Perdue et al. 1975), were characterized with respect to their amino acid content, DNA content, and role in the maturat...


    pub_type: 杂志文章


    authors: Perdue ML,Cohen JC,Randall CC,O'Callaghan DJ

    更新日期:1976-10-01 00:00:00

  • Alfalfa mosaic virus temperature-sensitive mutants. V. The nucleotide sequence of TBTS 7 RNA 3 shows limited nucleotide changes and evidence for heterologous recombination.

    abstract::Nucleotide sequence determination of the coat protein cistron of the alfalfa mosaic virus (AIMV) temperature-sensitive mutant, Tbts 7 (uv) revealed a small number of point mutations of which only one results in the replacement of an amino acid: the asparagine residue at position 126 is replaced by an aspartate residue...


    pub_type: 杂志文章


    authors: Huisman MJ,Cornelissen BJ,Groenendijk CF,Bol JF,van Vloten-Doting L

    更新日期:1989-08-01 00:00:00

  • Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV.

    abstract::Nucleotide sequence analysis of the genome of the baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) identified 18 homologues of the Autographa californica NPV (AcNPV) lefs (late expression factor genes). These BmNPV lefs showed high (73-98%) amino acid sequence identities to AcNPV lefs and were localized to s...


    pub_type: 杂志文章


    authors: Gomi S,Zhou CE,Yih W,Majima K,Maeda S

    更新日期:1997-03-31 00:00:00

  • Cloning and expression of NDV hemagglutinin-neuraminidase cDNA in a baculovirus expression vector system.

    abstract::The hemagglutinin-neuraminidase (HN) gene of the Hitchner B1 strain of Newcastle disease virus (NDV) was cloned as a cDNA and inserted into a baculovirus expression vector. The recombinant HN (recHN) expressed in Spodoptera frugiperda cells had both hemagglutinating and neuraminidase activities both of which were inhi...


    pub_type: 杂志文章


    authors: Nagy E,Derbyshire JB,Dobos P,Krell PJ

    更新日期:1990-06-01 00:00:00

  • Geographic classification of dengue-2 virus strains by antigen signature analysis.

    abstract::Dengue-2 virus strains from different locations were compared by T1-RNAse-resistant oligonucleotide fingerprinting and antigen signature analysis. The latter technique involved construction of radioimmunoassays using monoclonal antibodies that recognize nine distinct dengue-2 type-specific and flavivirus cross-reactiv...


    pub_type: 杂志文章


    authors: Monath TP,Wands JR,Hill LJ,Brown NV,Marciniak RA,Wong MA,Gentry MK,Burke DS,Grant JA,Trent DW

    更新日期:1986-10-30 00:00:00

  • Human immunodeficiency virus type 1 Nef protein mediates neural cell death: a neurotoxic role for IP-10.

    abstract::HIV-1 Nef is expressed in astrocytes, but a contribution to neuropathogenesis and the development of HIV-associated dementia (HAD) remains uncertain. To determine the neuropathogenic actions of the HIV-1 Nef protein, the brain-derived (YU-2) and blood-derived (NL4-3) Nef proteins were expressed in neural cells using a...


    pub_type: 杂志文章


    authors: van Marle G,Henry S,Todoruk T,Sullivan A,Silva C,Rourke SB,Holden J,McArthur JC,Gill MJ,Power C

    更新日期:2004-11-24 00:00:00

  • The complete genome structure and synthesis of infectious RNA from clones of tomato bushy stunt virus.

    abstract::The complete sequence of the genome of the cherry strain of tomato bushy stunt virus (TBSV), a member of the tombusvirus group, was determined. A full-length clone of the genome containing a bacteriophage T7 RNA polymerase promoter was assembled from partial cDNA clones. In vitro transcripts of the genome, either with...


    pub_type: 杂志文章


    authors: Hearne PQ,Knorr DA,Hillman BI,Morris TJ

    更新日期:1990-07-01 00:00:00

  • Identification of the 5' end of the rubella virus subgenomic RNA.

    abstract::The 5' end of the subgenomic RNA of rubella virus was determined by primer extension. The Maxam-Gilbert sequence ladder of the primer extension product contained a determinable sequence which was colinear with the complement of the sequence of the genomic RNA through nucleotide 3325 from the 3' end of the genomic RNA ...


    pub_type: 杂志文章


    authors: Frey TK,Marr LD,Sanchez A,Simmons RB

    更新日期:1989-01-01 00:00:00

  • The C-terminal tail of the gp41 transmembrane envelope glycoprotein of HIV-1 clades A, B, C, and D may exist in two conformations: an analysis of sequence, structure, and function.

    abstract::In addition to the major ectodomain, the gp41 transmembrane glycoprotein of HIV-1 is now known to have a minor ectodomain that is part of the long C-terminal tail. Both ectodomains are highly antigenic, carry neutralizing and non-neutralizing epitopes, and are involved in virus-mediated fusion activity. However, data ...


    pub_type: 杂志文章


    authors: Hollier MJ,Dimmock NJ

    更新日期:2005-07-05 00:00:00

  • Disruption of NF-kappa B signaling and chemokine gene activation by retroviral mediated expression of IKK gamma/NEMO mutants.

    abstract::Phosphorylation of I kappa Bs--the cytoplasmic inhibitors of the NF-kappa B transcription factors--is the key event which triggers activation of the NF-kappa B cascade. Signal-mediated phosphorylation of I kappa B alpha is mediated by a multiprotein complex, the I kappa B kinase (IKK) complex, which is composed of at ...


    pub_type: 杂志文章


    authors: Le Page C,Popescu O,Génin P,Lian J,Paquin A,Galipeau J,Hiscott J

    更新日期:2001-08-01 00:00:00

  • Cucumber mosaic virus-induced RNA replicase: solubilization and partial purification of the particulate enzyme.

    abstract::Cucumber seedlings infected with cucumber mosaic virus (CMV) contain a virus-induced RNA-dependent RNA polymerase (RNA replicase) in both soluble and particulate fractions of plant extracts; no such activity is found in uninfected plants. The extensive purification and characterization of the soluble RNA replicase has...


    pub_type: 杂志文章


    authors: Gill DS,Kumarasamy R,Symons RH

    更新日期:1981-08-01 00:00:00

  • In hepatocytes infected with duck hepatitis B virus, the template for viral RNA synthesis is amplified by an intracellular pathway.

    abstract::During the productive phase of chronic hepadnaviral infections, virion DNA synthesis occurs in the cytoplasm of the infected hepatocyte, but viral RNA is synthesized in the nucleus, apparently from a covalently closed, circular (CCC) viral DNA. J. Tuttleman, C. Pourcel, and J. Summers (1986a, Cell 47, 451-460) have sh...


    pub_type: 杂志文章


    authors: Wu TT,Coates L,Aldrich CE,Summers J,Mason WS

    更新日期:1990-03-01 00:00:00

  • Reactivities of human sera with human herpesvirus-8-infected BCBL-1 cells and identification of HHV-8-specific proteins and glycoproteins and the encoding cDNAs.

    abstract::The reactivates of human sera with uninduced and phorbol ester (TPA)-induced human herpesvirus-8 (HHV-8)-infected BCBL-1 cells were examined by immunofluorescence assay (IFA) and by radioimmunoprecipitation reactions (RIP). The seroprevalence of HHV-8 infections is low in the United States general population and only ...


    pub_type: 杂志文章


    authors: Chandran B,Smith MS,Koelle DM,Corey L,Horvat R,Goldstein E

    更新日期:1998-03-30 00:00:00

  • Tumorigenicity of adenovirus-transformed cells: region E1A of adenovirus 12 confers resistance to natural killer cells.

    abstract::Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12E1AB virus (containing E1A and E1B...


    pub_type: 杂志文章


    authors: Sawada Y,Föhring B,Shenk TE,Raska K Jr

    更新日期:1985-12-01 00:00:00