Abstract:
:Turnip leaves infected with turnip crinkle virus accumulate a 35-kd polypeptide which comigrates with the major protein from isolated virions. RNA from TCV virions directs the synthesis of a number of polypeptides in vitro including a 35-kd protein which is immunoprecipitable with antiserum to virus particles. Translation of viral RNA size-fractionated on sucrose gradients or methyl mercurichydroxide-containing gels shows that the TCV coat protein is synthesized primarily from RNA fragments which are smaller than the genomic RNA. A satellite RNA species found in virions does not direct the synthesis in vitro of any identifiable protein.
journal_name
Virologyjournal_title
Virologyauthors
Altenbach SB,Howell SHdoi
10.1016/0042-6822(82)90326-9subject
Has Abstractpub_date
1982-04-15 00:00:00pages
128-35issue
1eissn
0042-6822issn
1096-0341pii
0042-6822(82)90326-9journal_volume
118pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::We have previously adapted an experimental system which supports autonomous replication of human papillomavirus-11 (HPV-11) genomic DNA in a human squamous carcinoma cell line (SCC-4) following electroporation of linearized viral DNA. This system allows evaluation of HPV-11 transcription in the context of replicating ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0298
更新日期:1996-06-01 00:00:00
abstract::Vesicular stomatitis virus (VSV) isolated from two independently established lines of persistently infected mouse L cells expressed an altered phenotype of RNA synthesis at 37 degrees, the temperature at which the persistently infected cultures were maintained (T.K. Frey and J.S. Youngner, 1982, J. Virol. 44, 167-174)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90260-5
更新日期:1984-07-15 00:00:00
abstract::Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90277-0
更新日期:1984-04-15 00:00:00
abstract::Cryphonectria parasitica, the causal agent of chestnut blight is controlled in Europe through natural spread of Cryphonectria hypovirus 1 (CHV1), a mycovirus able to induce hypovirulence to the host. In recent years C. parasitica was reported infecting Azerbaijani population of chestnut, but the presence of CHV1 still...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.10.008
更新日期:2021-01-15 00:00:00
abstract::In this study, we show how the red spotted grouper nervous necrosis virus (RGNNV) causes loss of mitochondrial membrane potential and promotes host secondary apoptotic necrosis. RGNNV viral proteins such as protein alpha (42 kDa) and protein A (110 kDa) were quickly expressed between 12 h and 24 h postinfection (p.i.)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.052
更新日期:2006-04-10 00:00:00
abstract::Poliovirus readily establishes a persistent infection in the K562-Mu erythroleukemia cell strain. In this study, three additional K562 cell strains were analyzed for their responses to poliovirus infection and found to be quite variable. K562 cells obtained from the ATCC established a persistent infection, similar to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1541
更新日期:1995-10-20 00:00:00
abstract::The epithelium of the cervix uteri has been reported to be frequently coinfected with both human papillomaviruses (HPV) and helper virus-dependent adenoassociated viruses (AAV). Seroepidemiological data suggest that AAV infection could inhibit cervical cancer that is caused by specific ("high-risk") types of papilloma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1256
更新日期:2002-02-01 00:00:00
abstract::Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.025
更新日期:2018-02-01 00:00:00
abstract::The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.04.046
更新日期:2004-08-01 00:00:00
abstract::Human noroviruses bind histo-blood group antigens (HBGAs) and this interaction is thought to be important for an infection. We identified two additional fucose-binding pockets (termed fucose-3/4 sites) on a genogroup II human (GII.10) norovirus-protruding (P) dimer using X-ray crystallography. Fucose-3/4 sites were lo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.04.006
更新日期:2015-09-01 00:00:00
abstract::We previously showed that reduced infectivity of HIV with incompletely processed capsid-spacer protein 1 (CA-SP1) is rescued by cellular activation or increased expression of HSP90AB1, a member of the cytosolic heat shock protein 90 family. Here we show that HSP90AB1 is present in HIV virions and that HSP90AB1, but no...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.005
更新日期:2013-02-05 00:00:00
abstract::The effect of 2-aminopurine (2AP), an inhibitor of the RNA-dependent P1/eIF-2 protein kinase, on the expression of the reovirus serotype 1 Lang strain S1 and S4 genes in transfected simian COS cells was examined. In the absence of 2AP, the s4-encoded sigma 3 gene product was expressed about five times more efficiently...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90235-j
更新日期:1990-05-01 00:00:00
abstract::Marek's Disease Virus (MDV) infects chickens via respiratory route and causes lymphomas in internal organs including gastrointestinal tract. MDV infection causes a shift in the gut microbiota composition. However, interactions between the gut microbiota and immune responses against MDV infection are not well understoo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.10.011
更新日期:2021-01-15 00:00:00
abstract::Marek's disease (MD) infection has been controlled effectively by vaccination using nononcogenic and/or attenuated oncogenic Marek's disease virus (MDV) vaccines. Thus far, there is little knowledge on the role of cell-mediated immune (CMI) responses during MDV infection or vaccination. To elucidate the importance of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0400
更新日期:1996-08-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The SARS-CoV genome is predicted to contain 14 functional open reading frames (ORFs). The first ORF (1a and 1b) encodes a large polyprotein that is cleaved into nonstructural proteins (nsp)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.04.029
更新日期:2007-09-30 00:00:00
abstract::The Nef protein of Simian immunodeficiency virus (SIV) associates with multiple T lymphocyte signaling proteins, including the T cell receptor (TCR) zeta chain. We demonstrate here that these interactions are conserved and highly specific. Nefs derived from genetically diverse strains of SIV (SIV(mac)239, SIV(smm)PBj,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1628
更新日期:2002-10-10 00:00:00
abstract::Early region 1 (E1) of the human adenoviruses has many intriguing properties which have prompted numerous mutational studies to help delineate and characterize the domains responsible for these functions. In mutational analyses being done currently, the E1 region is usually cloned into a bacterial plasmid where it is ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90302-9
更新日期:1988-04-01 00:00:00
abstract::The management of hand-foot-and-mouth disease(HFMD) epidemic is difficult due to the frequent emergence of non-EV71 and non-CVA16 enteroviruses and some cases testing negative for HFMD-associated causative agents. To clarify the virus spectrum of mild and severe HFMD, a comprehensive virome analysis of 238 samples was...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.02.004
更新日期:2018-05-01 00:00:00
abstract::The purified genomic dsRNA of bluetongue virus type 1 (BTV 1) was separated into 10 size classes by polyacrylamide gel electrophoresis. These genome segments were recovered individually from the gel, denatured with methylmercuric hydroxide and translated in vitro in a rabbit reticulocyte lysate system. The virus-speci...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90131-4
更新日期:1984-05-01 00:00:00
abstract::The mechanism of vaccinia virus-induced selective inhibition of host cell protein synthesis was studied in a nonpermissive (Chinese hamster ovary, CHO) and in a permissive mouse cell line ( L cells). Small polyadenylated RNAs obtained from uninfected and infected cells were fractionated into six size classes by polyac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90135-e
更新日期:1990-12-01 00:00:00
abstract::We investigated the origin and the effect of insertion D67D-THGERDLGPA within HIV-1 RT from a patient failing antiviral therapy. The insertion developed within the context of pre-existing NRTI and NNRTI mutations (M41L, L210W, T215Y and N348I). Concurrently, the NRTI mutations T69I and V118I and the NNRTI mutations K1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.03.023
更新日期:2014-05-01 00:00:00
abstract::Efforts to generate a vaccine to prevent infection by human immunodeficiency virus (HIV) have focused on inducing neutralizing antibodies. However, cytotoxic T-lymphocyte (CTL) responses are a major immune defense mechanism required for recovery from many different virus infections. Since CTL epitopes can be defined b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90504-i
更新日期:1992-06-01 00:00:00
abstract::Human cytomegalovirus U(L)112-113 encodes four phosphoproteins, pp84, pp50, pp43, and pp34, with common amino-termini. A previous report by Kerry et al. (J. Virol. 70, 373-382, 1996) demonstrated that U(L)112-113 products activate U(L)54 promoter in cooperation with immediate-early (IE) proteins. In this study, we ide...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9800
更新日期:1999-07-20 00:00:00
abstract::We recently developed an in vitro transcapsidation system in which infectivity of single-shelled (ss) rotavirus particles was successfully rescued (Chen and Ramig, Virology [1993]). Here, we report the rescue of infectivity of rotavirus core particles using virus strain B223 (G serotype 10) as the core donor and strai...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1315
更新日期:1993-06-01 00:00:00
abstract::Alphaherpesviruses comprise closely related viruses of man and animal, including herpes simplex virus, varicella-zoster virus and pseudorabies virus (PRV). Here, using methyl-beta-cyclodextrin and fluorescently tagged PRV, we directly show that depletion of cholesterol from the plasma membrane of host cells significan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.039
更新日期:2008-07-05 00:00:00
abstract::RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus multiplication based on ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.051
更新日期:2005-05-25 00:00:00
abstract::The monoclonal antibody 2D5 neutralized vaccinia virus by preventing penetration of the virus and reacting with VP23-29K. The conformation of the VP23-29K was maintained by a disulfide bond(s), and the 2D5mAb reacted stronger with the nonreduced 23-kDa form than with the reduced 29-kDa form. We selected several escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0337
更新日期:1996-06-15 00:00:00
abstract::The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9046
更新日期:1998-03-30 00:00:00
abstract::Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monome...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8445
更新日期:1997-03-03 00:00:00
abstract::Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.02.018
更新日期:2017-05-01 00:00:00