Abstract:
:The effect of 2-aminopurine (2AP), an inhibitor of the RNA-dependent P1/eIF-2 protein kinase, on the expression of the reovirus serotype 1 Lang strain S1 and S4 genes in transfected simian COS cells was examined. In the absence of 2AP, the s4-encoded sigma 3 gene product was expressed about five times more efficiently than the s1-encoded sigma 1 gene product. When COS cells were treated with 2AP, the synthesis of the sigma 1 polypeptide was increased about fivefold compared to that in untreated cells even though s1 mRNA levels were not detectably altered. In contrast to the increased translational efficiency of the s1 mRNA observed in 2AP-treated cells, the translational efficiency of the s4 mRNA was not affected by 2AP treatment. However, the cytoplasmic accumulation of s4 mRNA was transiently decreased by 2AP treatment. These results demonstrate that the expression of the reovirus S1 and S4 genes in transient transfection assays is differentially affected by 2AP. Furthermore, when considered together with the prior observation that the reovirus s1 mRNA is a potent activator of the RNA-dependent protein kinase relative to the s4 mRNA which is a very poor activator, the results are consistent with the suggestion that the differential translational efficiency of the reovirus s1 and s4 mRNAs in vivo may be attributed in part to their differential ability to activate the P1/eIF-2 protein kinase.
journal_name
Virologyjournal_title
Virologyauthors
Samuel CE,Brody MSdoi
10.1016/0042-6822(90)90235-jsubject
Has Abstractpub_date
1990-05-01 00:00:00pages
106-13issue
1eissn
0042-6822issn
1096-0341journal_volume
176pub_type
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