Betanodavirus induces phosphatidylserine exposure and loss of mitochondrial membrane potential in secondary necrotic cells, both of which are blocked by bongkrekic acid.

abstract：:Mouse hepatitis virus strain A59 (MHV-A59) encodes within the 22-kb gene 1 a large polyprotein containing three proteinase domains with proven or predicted cysteine catalytic residues. E64d, a specific, irreversible inhibitor of cysteine (thiol) proteinases, inhibits the processing of the gene 1 polyprotein. Specifica...

journal_title：Virology

authors： Kim JC,Spence RA,Currier PF,Lu X,Denison MR

更新日期：1995-04-01 00:00:00

abstract：:The baculovirus-insect cell system is used routinely for foreign glycoprotein production, but the precise nature of the N-glycosylation pathway in this system remains unclear. Some studies indicate that these cells cannot process N-linked oligosaccharides to complex forms containing outer-chain galactose and sialic ac...

journal_title：Virology

authors： Jarvis DL,Finn EE

更新日期：1995-10-01 00:00:00

abstract：:Poliovirus was first isolated nearly 100 years ago in a landmark experiment that established the viral etiology of poliomyelitis. This discovery stimulated investigation of the pathogenesis of poliomyelitis in many laboratories. Nearly 50 years later, when two effective poliovirus vaccines were developed, the impetus ...

journal_title：Virology

authors： Racaniello VR

更新日期：2006-01-05 00:00:00

abstract：:TMV capsid protein reacts with and encapsidates many of the chloroplast DNA transcripts both in vivo and in vitro to form pseudovirions. We report on the encapsidation initiation reaction with one of the major RNA species found in in vivo formed pseudovirions, the mRNA for the chloroplast-encoded large subunit of ribu...

journal_title：Virology

authors： Atreya CD,Siegel A

更新日期：1989-02-01 00:00:00

abstract：:Vaccinia virus gene D12L, which lies between nucleotides 14,350 and 13,487 in the HindIII D fragment, is transcribed at early times in infection and is capable of encoding a protein 287 amino acids in length with a predicted molecular mass of 33,331. A polyclonal antiserum was raised in rabbits to a fusion protein con...

journal_title：Virology

authors： Niles EG,Lee-Chen GJ,Shuman S,Moss B,Broyles SS

更新日期：1989-10-01 00:00:00

abstract：:We analyzed various mutants of tomato leaf curl virus-India to investigate the role of ORFs AV3, AV2, and coat protein (CP) in viral replication, movement, and symptom development. The results of these studies indicate that ORF AV3 does not encode a protein. Plants inoculated with infectious DNA which contained deleti...

journal_title：Virology

authors： Padidam M,Beachy RN,Fauquet CM

更新日期：1996-10-15 00:00:00

abstract：:The full-length E2 protein of human papillomavirus type 16 is believed to act as a trans-repressor of the viral p97 promoter. Previous reports have provided evidence that transcripts with the potential to encode the E2 protein contain the 880/2708 splice junction. We have further analyzed the structure of the E2-encod...

journal_title：Virology

authors： Sherman L,Alloul N

更新日期：1992-12-01 00:00:00

abstract：:Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cell...

journal_title：Virology

authors： Desport M,Tenaya IW,McLachlan A,McNab TJ,Rachmat J,Hartaningsih N,Wilcox GE

更新日期：2009-10-25 00:00:00

abstract：:In this study we show that macrophages (Mphi) are latently infected with murine cytomegalovirus (MCMV). After clearance of acute MCMV infection, the predominant form of chronic infection in Balb mice is latency rather than persistence. Peritoneal exudate cells (PECs) from latently infected Balb mice (3-9 months postin...

journal_title：Virology

authors： Pollock JL,Presti RM,Paetzold S,Virgin HW 4th

更新日期：1997-01-06 00:00:00

abstract：:Cell-to-cell and long-distance transport of some plant viruses requires coordinated action of three movement proteins encoded by triple gene block (TGB). The largest of TGB proteins, TGBp1, is a member of the superfamily I of DNA/RNA helicases and possesses a set of conserved helicase sequence motifs necessary for vir...

journal_title：Virology

authors： Kalinina NO,Rakitina DV,Solovyev AG,Schiemann J,Morozov SY

更新日期：2002-05-10 00:00:00

abstract：:Herpesvirus glycoprotein C (gC) functions as a major virus attachment protein. The gC sequence of the neurovirulent bovine herpesvirus type 5 (BHV-5) virus was determined and compared with the gC sequence of the nonneurovirulent BHV-1. Alignment of the predicted amino acid sequences of BHV-1 and BHV-5 gC ORFs showed t...

journal_title：Virology

authors： Chowdhury SI

更新日期：1995-11-10 00:00:00

abstract：:Electron microscopy and sequence analysis have suggested the presence of distinct morphological regions within the reovirus cell attachment protein sigma 1. Kinking of purified sigma 1 observed by electron microscopy and sequence-predicted flexibility profiles suggest the presence of potential flexible regions in the ...

journal_title：Virology

authors： Duncan R,Lee PW

更新日期：1994-08-15 00:00:00

abstract：:Influenza antiviral drugs that use protein inhibitors can lose their efficacy as resistant strains emerge. As an alternative strategy, we investigated the use of small interfering RNA molecules (siRNAs) by characterizing three siRNAs (M747, M776 and M832) targeting the influenza matrix 2 gene and three (NS570, NS595 a...

journal_title：Virology

authors： McMillen CM,Beezhold DH,Blachere FM,Othumpangat S,Kashon ML,Noti JD

更新日期：2016-10-01 00:00:00

abstract：:To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...

journal_title：Virology

authors： Andino Fde J,Grayfer L,Chen G,Chinchar VG,Edholm ES,Robert J

更新日期：2015-11-01 00:00:00

abstract：:Viruses are infectious particles whose viability is dependent on the cells of living organisms, such as bacteria, plants, and animals. It is of great interest to discover how viruses function inside host cells in order to develop therapies to treat virally infected organisms. The fruit fly Drosophila melanogaster is a...

journal_title：Virology

authors： Hughes TT,Allen AL,Bardin JE,Christian MN,Daimon K,Dozier KD,Hansen CL,Holcomb LM,Ahlander J

更新日期：2012-02-05 00:00:00

abstract：:Plant virus-based vectors provide valuable tools for expression of foreign proteins in plants and for gene function studies. None of the presently available virus vectors is suitable for use in soybean. In the present study, we produced Bean pod mottle virus (BPMV)-based vectors that are appropriate for gene expressio...

journal_title：Virology

authors： Zhang C,Ghabrial SA

更新日期：2006-01-20 00:00:00

abstract：:The HCMV IE2 protein promiscuously activates transcription of many viral and cellular genes. IE2 also negatively autoregulates its own expression by binding to a strategically positioned IE2 binding site, called CRS, located immediately downstream of the TATA box of the HCMV major IE promoter. Here we show that IE2 is...

journal_title：Virology

authors： Tsai HL,Kou GH,Tang FM,Wu CW,Lin YS

更新日期：1997-11-24 00:00:00

abstract：:Stress granule (SG)-like antiviral granules (AVG) had been found in some vaccinia virus infection conditions and shown to repress translation. Similar RNA granules are also associated with translational inhibition and poxvirus restriction mediated by the host restriction factor SAMD9, but their function is less clear....

journal_title：Virology

authors： Meng X,Xiang Y

更新日期：2019-03-01 00:00:00

abstract：:After infection with mumps virus, cellular ribonucleic acid synthesis of a murine lymphoma cell line, EL4, was appreciably depressed. The inactivation of viral infectivity by ultraviolet irradiation or the treatment of cells with mouse interferon did not abolish the inhibiting effect, suggesting that virus replication...

journal_title：Virology

authors： Yamada A,Tsurudome M,Hishiyama M,Ito Y

更新日期：1984-06-01 00:00:00

abstract：:The complete nucleotide sequence of the nucleocapsid (N) gene of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus of plant rhabdoviruses, has been determined from cDNA clones of both the viral genomic and messenger RNAs. The previously identified N gene was adjacent to the 3' leader ...

journal_title：Virology

authors： Wetzel T,Dietzgen RG,Geering AD,Dale JL

更新日期：1994-08-01 00:00:00

abstract：:Plant virus-encoded movement protein(s) (MP), and for many viruses the coat protein (CP), is required to mediate viral spread between plant cells via plasmodesmata (PD). Most probably, the genomic RNA of potexviruses moves through PD as assembled virions and/or as ribonucleoprotein complexes containing the CP and 25-k...

journal_title：Virology

authors： Atabekov JG,Rodionova NP,Karpova OV,Kozlovsky SV,Poljakov VY

更新日期：2000-06-05 00:00:00

abstract：:Attempts to define the genetic determinants required for efficient growth of human immunodeficiency virus type 1 (HIV-1) in monocyte-macrophages were made by constructing chimeras between two infectious clones of HIV-1 (HXB2 and LW/C), which despite only minor differences in their DNA sequence have striking difference...

journal_title：Virology

authors： Malykh A,Reitz MS Jr,Louie A,Hall L,Lori F

更新日期：1995-01-10 00:00:00

abstract：:To determine the direction of the entry of DNA during in vitro bacteriophage T7 DNA packaging, incompletely packaged DNA (ipDNA) was fractionated by agarose gel electrophoresis after degradation of DNA outside of capsids and then release of packaged DNA from capsids. After fractionation, quantitative in-gel probing wi...

journal_title：Virology

authors： Son M,Watson RH,Serwer P

更新日期：1993-09-01 00:00:00

abstract：:Human immunodeficiency virus (HIV) Gag proteins are assembled into virus particles and then cleaved by the virion-associated HIV protease. Concomitant with Gag processing, doughnut-like HIV particles (the immature form) are converted to particles containing condensed cores (the mature form). Here we describe the in vi...

journal_title：Virology

authors： Morikawa Y,Shibuya M,Goto T,Sano K

更新日期：2000-07-05 00:00:00

abstract：:Functions of the six Epstein-Barr virus latent infection nuclear proteins (EBNA-1, -2, -3A, -3B, -3C, or -LP) in maintaining latent infection or cell growth transformation are only partially understood. Using antibodies specific for each EBNA in immunofluorescence microscopy, EBNA-2, -3A, and -3C localized to subnucle...

journal_title：Virology

authors： Petti L,Sample C,Kieff E

更新日期：1990-06-01 00:00:00

abstract：:The immediate-early transcripts of pseudorabies virus have been located in a region of the genome situated internally within the inverted repeat between map positions 0.99 and 0.95. A single immediate-early transcript (approximately 6 kb) can be detected both in the cytoplasmic and nuclear fractions of infected, cyclo...

journal_title：Virology

authors： Ihara S,Feldman L,Watanabe S,Ben-Porat T

更新日期：1983-12-01 00:00:00

abstract：:HIV-1 transmission is usually initiated by a single viral strain called transmitted/ founder (T/F) virus. In in vitro models, HIV-1 can efficiently spread via cell-free and virological synapse (VS)-mediated cell-to-cell infection. Both modes of infection require the viral glycoprotein Envelope (Env). The efficiency wi...

journal_title：Virology

authors： Li H,Chen BK

更新日期：2019-01-02 00:00:00

abstract：:In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage mat...

journal_title：Virology

authors： Behnisch W,Schmieger H

更新日期：1985-07-30 00:00:00

abstract：:Expression of the SV40 large T-antigen allows primary cells to escape senescence and thereby become immortalized. Immortalization occurs in two steps, extension of life span and acquisition of unlimited cell division potential. By following the increase in expression of a senescence-associated marker with increased ce...

journal_title：Virology

authors： Tevethia MJ,Lacko HA,Conn A

更新日期：1998-04-10 00:00:00

abstract：:Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 1...

journal_title：Virology

authors： Pugh JC,Summers JW

更新日期：1989-10-01 00:00:00