RNA helicase activity of the plant virus movement proteins encoded by the first gene of the triple gene block.

abstract：:Hepatitis C virus (HCV) core protein is a multifunctional protein interacting with cellular and viral proteins and promoters. A tetracycline-regulated system was used to generate a HepG2 Tet-Off cell line allowing regulated expression of a full-length (191 aa) and an N(c)-truncated core protein (160 aa). In this syste...

journal_title：Virology

authors： Erhardt A,Hassan M,Heintges T,Häussinger D

更新日期：2002-01-20 00:00:00

abstract：:Matrix protein M1 purified from influenza A and B viruses has been analyzed for its ability to specifically interact with cellular proteins by immune coprecipitation and by an in vitro binding assay on nitrocellulose on PVDF membranes. When M1 was mixed with lysates of uninfected cells there was selective binding of h...

journal_title：Virology

authors： Zhirnov OP,Klenk HD

更新日期：1997-09-01 00:00:00

abstract：:The v-erb B protein of avian erythroblastosis virus (AEV) possesses an associated protein kinase activity in vitro. Analysis of temperature-sensitive mutants, and nonconditional host range mutants of AEV demonstrated that there was no simple correlation between this autophosphorylation activity and the transformation ...

journal_title：Virology

authors： Hayman MJ,Kitchener G,Knight J,McMahon J,Watson R,Beug H

更新日期：1986-04-15 00:00:00

abstract：:Coxsackievirus B4 (CBV4), a member of the Picornavirus genus, has long been implicated in the development of insulin-dependent diabetes mellitus (IDDM), by viral-induced pancreatic cell damage. Although the pancreotropic nature of this virus is well documented, the early stages of CBV4 viral infection that involve the...

journal_title：Virology

authors： Triantafilou K,Triantafilou M

更新日期：2004-08-15 00:00:00

abstract：:The most recent introduction of an avian influenza A virus without reassortment into mammals occurred in 1979 when H1N1 strains could be isolated from diseased pigs in northern Europe. This newly introduced avian virus formed a stable lineage in pigs and, in the meantime, spread all over Europe. In 1991 highly pathoge...

journal_title：Virology

authors： Ludwig S,Haustein A,Kaleta EF,Scholtissek C

更新日期：1994-07-01 00:00:00

abstract：:The synthesis and processing of the nucleocapsid protein (pp60) of the JHM strain of murine coronaviruses were examined. Pulse-chase experiments showed that pp60 was synthesized initially as a protein of approximately 57,000 in molecular weight (p57). Immunoprecipitation using mouse anti-JHMV antiserum indicated that ...

journal_title：Virology

authors： Stohlman SA,Fleming JO,Patton CD,Lai MM

更新日期：1983-10-30 00:00:00

abstract：:The virion RNA of mouse hepatitis virus, strain A59 (MHV-A59) is believed to be the mRNA for the viral RNA-dependent RNA polymerase. The cell-free translation of virion RNA results in the synthesis of two predominant products p220 and p28 (M. R. Denison and S. Perlman, 1986, J. Virol. 60, 12-18). p28 is a basic protei...

journal_title：Virology

authors： Denison M,Perlman S

更新日期：1987-04-01 00:00:00

abstract：:We previously demonstrated in trials of a variety of experimental vaccines to equine infectious anemia virus (EIAV) a remarkable spectrum of efficacy ranging from sterilizing protection to severe enhancement of virus replication and disease, depending on the immunization strategy used. This range of vaccine efficacy o...

journal_title：Virology

authors： Hammond SA,Raabe ML,Issel CJ,Montelaro RC

更新日期：1999-09-30 00:00:00

abstract：:The P150 and P90 replicase proteins of rubella virus (RUBV), a plus-strand RNA Togavirus, produce a unique cytoplasmic fiber network resembling microtubules. Pharmacological and mutagenic approaches were used to determine if these fibers functioned in virus replication. The pharmacological approach revealed that micro...

journal_title：Virology

authors： Matthews JD,Tzeng WP,Frey TK

更新日期：2010-10-25 00:00:00

abstract：:We previously demonstrated that human parainfluenza virus type 2 (hPIV-2) induces RhoA activation, which promotes its growth. RhoA controls the equilibrium between globular and filamentous actin (F-actin). We found that F-actin formation is induced by wild type (wt) hPIV-2 infection, and that inhibition of F-actin for...

journal_title：Virology

authors： Ohta K,Matsumoto Y,Yumine N,Nishio M

更新日期：2018-11-01 00:00:00

abstract：:To determine the direction of the entry of DNA during in vitro bacteriophage T7 DNA packaging, incompletely packaged DNA (ipDNA) was fractionated by agarose gel electrophoresis after degradation of DNA outside of capsids and then release of packaged DNA from capsids. After fractionation, quantitative in-gel probing wi...

journal_title：Virology

authors： Son M,Watson RH,Serwer P

更新日期：1993-09-01 00:00:00

abstract：:Hepatitis C virus (HCV) was shown to activate protein kinase R (PKR), which inhibits expression of interferon (IFN) and IFN-stimulated genes by controlling the translation of newly transcribed mRNAs. However, it is unknown exactly how HCV activates PKR. To address the molecular mechanism(s) of PKR activation mediated ...

journal_title：Virology

authors： Suzuki R,Matsuda M,Shimoike T,Watashi K,Aizaki H,Kato T,Suzuki T,Muramatsu M,Wakita T

更新日期：2019-03-01 00:00:00

abstract：:We report here the nonenzymatic self-ligation of transcripts corresponding to the peach latent mosaic viroid (PLMVd). This is the first description of this process with viroid sequences, although it has been reported to occur with human hepatitis delta virus RNA. Self-ligation occurs when the 5'-hydroxyl and the 2',3'...

journal_title：Virology

authors： Lafontaine D,Beaudry D,Marquis P,Perreault JP

更新日期：1995-10-01 00:00:00

abstract：:Begomoviruses (family Geminiviridae) are responsible for many economically important crop diseases worldwide. The majority of these diseases are caused by bipartite begomovirus infections, although a rapidly growing number of diseases of the Old World are associated with monopartite begomoviruses. With the exception o...

journal_title：Virology

authors： Briddon RW,Stanley J

更新日期：2006-01-05 00:00:00

abstract：:The function of a substantial percentage of the putative protein-coding open reading frames (ORFs) in viral genomes is unknown. As their sequence is not similar to that of proteins of known function, the function of these ORFs cannot be assigned on the basis of sequence similarity. Methods complement or in combination...

journal_title：Virology

authors： Han LY,Cai CZ,Ji ZL,Chen YZ

更新日期：2005-01-05 00:00:00

abstract：:A full-length cDNA clone of the U1 (common) strain of tobacco mosaic virus (TMV) was constructed, and highly infectious transcripts were produced in vitro using bacteriophage T7 RNA polymerase. Frameshift mutations designed to cause premature termination of translation were introduced into either the 30-kDa movement p...

journal_title：Virology

authors： Holt CA,Beachy RN

更新日期：1991-03-01 00:00:00

abstract：:The genomic S RNA segment of Prospect Hill virus (PHV), a member of the Hantavirus genus, was molecularly cloned and the nucleotide sequence of the cDNA determined. The PHV S RNA segment is 1675 nucleotides long. A long open reading frame was identified in the viral complementary-sense RNA that could encode a 433 amin...

journal_title：Virology

authors： Parrington MA,Kang CY

更新日期：1990-03-01 00:00:00

abstract：:We have carried out an exhaustive search for amino acid sequence similarities between vesicular stomatitis virus (VSV) proteins and database entries. Unexpectedly, we found that the L polymerase protein contains two blocks of sequence (residues 725-1102 and 1291-1671) with distant but statistically significant similar...

journal_title：Virology

authors： McClure MA,Perrault J

更新日期：1989-09-01 00:00:00

abstract：:Cells transformed with specific regions of the foot-and-mouth disease virus (FMDV) genome have been constructed and analyzed with respect to viability and susceptibility to FMDV infection. Constitutive expression of an active protease 3C under the control of the tk promoter has been documented by the ability of transf...

journal_title：Virology

authors： Martínez-Salas E,Domingo E

更新日期：1995-09-10 00:00:00

abstract：:Characterization of the regions of human papillomaviruses (HPVs) that elicit neutralizing immune responses supports studies on viral infectivity and provides insight for the development and evaluation of prophylactic vaccines. HPV11 is a major etiologic agent of genital warts and a likely vaccine candidate. A conforma...

journal_title：Virology

authors： Ludmerer SW,McClements WL,Wang XM,Ling JC,Jansen KU,Christensen ND

更新日期：2000-01-20 00:00:00

abstract：:Simian virus (SV40) T antigen shares many characteristics with adenovirus E1A which is known to induce apoptosis. To verify the potential of SV40 T antigen-mediated apoptosis, we stably expressed T antigen in immortalized human epithelial cells (Z172 and HaCaT). We found that SV40 T antigen could directly cause apopto...

journal_title：Virology

authors： Chen SL,Tsao YP,Chen YL,Huang SJ,Chang JL,Wu SF

更新日期：1998-05-10 00:00:00

abstract：:To examine whether there are HBV variants not yet described which cannot express HBe due to a mutation in the pre-C region, and, if they exist, whether they cause a particular course of infection and disease, we analyzed the HBV genomes of a HBs/anti-HBe positive chronic carrier who had several episodes of acute reexa...

journal_title：Virology

authors： Raimondo G,Schneider R,Stemler M,Smedile V,Rodino G,Will H

更新日期：1990-11-01 00:00:00

abstract：:The virion-associated Vpr protein of human immunodeficiency virus type 1 (HIV-1) alters cell cycle progression from the G2 phase, influences the virus in vivo mutation rate, and participates in the nuclear translocation of viral DNA. While many Vpr-interacting proteins have been identified, the functional relevance of...

journal_title：Virology

authors： Mansky LM,Preveral S,Le Rouzic E,Bernard LC,Selig L,Depienne C,Benarous R,Benichou S

更新日期：2001-03-30 00:00:00

abstract：:Little is known concerning the function of the C-terminal nonconserved region of Sindbis virus nsP3. In this report, we created a number of in-frame deletions and duplications (from 12 to 159 residues) in this region and examined their effects on Sindbis virus replication. Sindbis RNA transcripts containing these muta...

journal_title：Virology

authors： Lastarza MW,Grakoui A,Rice CM

更新日期：1994-07-01 00:00:00

abstract：:Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-kappaB). The activation of NF-kappaB has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a tra...

journal_title：Virology

authors： Reimers K,Buchholz K,Werchau H

更新日期：2005-01-20 00:00:00

abstract：:Cell-free extracts of Drosophila melanogaster cells infected with FHV (Flockhouse virus) have the capacity to synthesize FHV RNA. When such extracts are incubated appropriately, ss and ds FHV RNA 1, -2, and -3 can be detected as synthesis products. Pulse-chase analyses indicate that radioactivity, incorporated into ds...

journal_title：Virology

authors： Wu SX,Kaesberg P

更新日期：1991-07-01 00:00:00

abstract：:Homologous, subgenomic fragments of the viral LCR and E6/E7 transforming genes of HPV-18 and HPV-16 were amplified from several primary cervical, penile, and vulvar tumors and cloned into a pUC-18-derived vector. When assayed by a quantitative transformation assay using primary human keratinocytes, the subgenomic regi...

journal_title：Virology

authors： Villa LL,Schlegel R

更新日期：1991-03-01 00:00:00

abstract：:The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two poly...

journal_title：Virology

authors： Liu DX,Inglis SC

更新日期：1992-01-01 00:00:00

abstract：:Human hepatitis B virus (HBV) envelopes contain three distinct glycoproteins called L, M, and S HBsAg. Each is posttranslationally modified to contain N-linked oligosaccharides. N-linked oligosaccharides, after attachment to a polypeptide backbone, are processed by enzymes within the endoplasmic reticulum (ER). There ...

journal_title：Virology

authors： Lu X,Mehta A,Dwek R,Butters T,Block T

更新日期：1995-11-10 00:00:00

abstract：:West Nile virus (WNV) is a flavivirus that swept rapidly across North America in 1999, declined in prevalence, and then resurged in 2012. To date, no vaccine is available to prevent infection in the human population. Herpes simplex virus (HSV) replication-defective vaccine vectors induce a durable immunity characteriz...

journal_title：Virology

authors： Taylor TJ,Diaz F,Colgrove RC,Bernard KA,DeLuca NA,Whelan SPJ,Knipe DM

更新日期：2016-09-01 00:00:00