Abstract:
:The genomic S RNA segment of Prospect Hill virus (PHV), a member of the Hantavirus genus, was molecularly cloned and the nucleotide sequence of the cDNA determined. The PHV S RNA segment is 1675 nucleotides long. A long open reading frame was identified in the viral complementary-sense RNA that could encode a 433 amino acid (49K) nucleocapsid (N) protein. Comparison with the sequence of the related Hantavirus (Hantaan 76-118) S RNA segment indicated that there was 57% nucleotide sequence homology between the two S RNA segments. A higher degree of conservation in amino acid sequence homology (62%) was observed in the N proteins of these viruses. At the N-terminus 147 of 225 amino acids are homologous, while approximately 82% of the 124 amino acids at the C-terminus are homologous between the two N proteins. The longest stretch of homologous amino acid sequence is found in this region, and is 17 amino acids in length. Also, many of the differences in amino acid sequence between the two N proteins resulted from conservative substitutions. Hydropathy plots of the two N proteins also reveal many similarities including a conserved potential antigenic site. Unlike Hantaan virus, a second smaller overlapping open reading frame was observed in the viral complementary-sense RNA of PHV and could potentially encode a 90 amino acid (10.5K) protein. Our data indicate that the N proteins of PHV and Hantaan virus are closely related despite divergence in the nucleotide sequence of their S RNA segments.
journal_name
Virologyjournal_title
Virologyauthors
Parrington MA,Kang CYdoi
10.1016/0042-6822(90)90197-ysubject
Has Abstractpub_date
1990-03-01 00:00:00pages
167-75issue
1eissn
0042-6822issn
1096-0341journal_volume
175pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::In this study, we present enzymatic differences found between recombinant RTs derived from feline leukemia virus and feline immunodeficiency virus. Firstly, FIV RT showed low steady state K(m) values for dNTPs compared to FeLV RT. Consistent with this, FIV RT synthesized DNA more efficiently than FeLV RT at low dNTP c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.02.010
更新日期:2005-04-25 00:00:00
abstract::The unordered N-termini of parvovirus capsid proteins (Nt) are translocated through a channel at the icosahedral five-fold axis to serve for virus traffick. Heterologous peptides were genetically inserted at the Nt of MVM to study their functional tolerance to manipulations. Insertion of a 5T4-single-chain antibody at...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.05.025
更新日期:2012-10-10 00:00:00
abstract::The epithelium of the cervix uteri has been reported to be frequently coinfected with both human papillomaviruses (HPV) and helper virus-dependent adenoassociated viruses (AAV). Seroepidemiological data suggest that AAV infection could inhibit cervical cancer that is caused by specific ("high-risk") types of papilloma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1256
更新日期:2002-02-01 00:00:00
abstract::Simian virus (SV40) T antigen shares many characteristics with adenovirus E1A which is known to induce apoptosis. To verify the potential of SV40 T antigen-mediated apoptosis, we stably expressed T antigen in immortalized human epithelial cells (Z172 and HaCaT). We found that SV40 T antigen could directly cause apopto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9109
更新日期:1998-05-10 00:00:00
abstract::N-tropic murine leukemia viruses have been observed in connection with radiation-induced osteosarcomagenesis in BALB/c mice. We have investigated the bone disease-inducing potential of molecularly cloned, BALB/c-derived N-tropic viruses in the random-bred NMRI mouse strain. The germ-line virus and an exogenous virus i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90171-m
更新日期:1990-12-01 00:00:00
abstract::Sera from 215 non-drug-injecting Toba and Mataco-Mataguayo pure Indians belonging to four communities in northern Argentina were examined using assays that allow differentiation between reactivities due to type-specific antigens of the human T-cell leukemia/lymphoma virus (HTLV). Three of these populations have very l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1631
更新日期:1993-12-01 00:00:00
abstract::The SV40 large T-antigen (TAg) has proven useful in studying pathways involved with cell division and tissue homeostasis. TAg disrupts the normal action of tumor suppressors pRb and p53. It is unclear whether T-antigen inhibition of p53 and pRb is sufficient for oncogenic transformation or if additional T-antigen acti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0866
更新日期:2001-04-25 00:00:00
abstract::Polyomaviral vectors are generated by transfecting 293T cells with three sets of DNAs: DNA for the expression of simian virus 40 (SV40) T antigen; DNA for the expression of SV40 capsid proteins, and vector DNA harboring a reporter gene expression cassette carrying a SV40 origin. The vector DNA harbors a minimal sequen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.032
更新日期:2008-09-15 00:00:00
abstract::We show that the product of the polarity suppression (psu) gene from bacteriophage P4 associates with P4 capsids. This association can occur when Psu is (i) provided in vivo from the P4 genome or from a plasmid or (ii) provided in vitro by mixing viable phage particles with Psu protein. Psu is unable to associate with...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90538-z
更新日期:1992-06-01 00:00:00
abstract::We have previously shown that peroxisome proliferator-activated receptor-gamma (PPARgamma) agonists inhibited the inflammatory response of RSV-infected human lung epithelial cells. In this study, we supply evidence that specific PPARgamma agonists (15d-PGJ2, ciglitazone, troglitazone, Fmoc-Leu) efficiently blocked the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.03.008
更新日期:2006-07-05 00:00:00
abstract::Retrons are unusual, reverse transcriptase-encoding elements found in bacteria. Although there are a number of indications that retrons are mobile elements, their transposition has not been observed. The Escherichia coli retrons Ec67 and Ec86 are different retrons inserted at the same site and we have further characte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0228
更新日期:1996-05-01 00:00:00
abstract::The close association between human papillomavirus type 16 and cervical cancer implies some role for the virus in the development of this cancer. It has been demonstrated that HPV-16 DNA sequences in the presence of activated ras are capable of transforming primary baby rat kidney cells. This communication describes t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90695-2
更新日期:1988-07-01 00:00:00
abstract::Our recent results demonstrated that bile acids facilitate virus escape from the endosomes into the cytoplasm for successful replication of porcine enteric calicivirus (PEC). We report a novel finding that bile acids can be substituted by cold treatment for endosomal escape and virus replication. This endosomal escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.04.022
更新日期:2015-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 subtype C (HIV-1C) accounts for about 50% of all HIV infections in the pandemic and is the predominant subtype in the heavily burdened region of southern Africa. HIV-1C possesses unique genetic and phenotypic features that might be associated with biological differences compared to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.047
更新日期:2006-04-10 00:00:00
abstract::The reticuloendotheliosis virus (REV) group of retroviruses and type D simian retroviruses (SRV) belong to the same receptor interference group. The cellular receptor for these viruses has not yet been identified. In order to study the distribution of the receptor and to identify a receptor negative cell line, vector ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1651
更新日期:1994-11-15 00:00:00
abstract::Coat protein-protected fragments of alfalfa mosaic virus RNA (AlMV-RNA) and tobacco streak virus RNA (TSV-RNA), which were isolated as described [D. Zuidema, M. F. A. Bierhuizen, B. J. C. Cornelissen, J. F. Bol, and E. M. J. Jaspars (1983)Virology, 125, 361-369], were tested for their ability to rebind AlMV coat prote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90165-x
更新日期:1984-07-30 00:00:00
abstract::To elucidate the mechanism of formation of cowpea mosaic virus (CPMV) particles, RNA-2-encoded precursor proteins were expressed in Spodoptera frugiperda cells. Processing of the 105K and 95K polyproteins in trans to give the mature Large (L) and Small (S) coat proteins required both the 32K proteinase cofactor and th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.023
更新日期:2009-10-25 00:00:00
abstract::The human T cell leukemia virus type I (HTLV-I) is an oncogenic retrovirus that is etiologically linked to the genesis of adult T cell leukemia (ATL) as well as HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Emerging evidence suggests that the pathogenicity of HTLV-I involves deregulated activati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.12.008
更新日期:2005-03-01 00:00:00
abstract::Cytotoxic T-cell clones raised against X-31 (H3N2) influenza virus in C57BL/6 mice can be directed against an influenza A virus subtype specific determinant (1). A representative T-cell clone (A3.1) has been used in combination with a set of genetically typed recombinant viruses, to show that the A/PR/8/34 nucleoprote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90413-6
更新日期:1984-03-01 00:00:00
abstract::The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.01.004
更新日期:2015-07-01 00:00:00
abstract::A nonhemagglutinating mutant of a 1978 isolate of canine parvovirus (CPV) was derived after repeated passages in the NLFK feline kidney cell line. The mutant CPV was antigenically indistinguishable from wild-type virus when tested with 82 monoclonal antibodies, and it replicated in cat and dog cell lines in culture. S...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90255-3
更新日期:1988-03-01 00:00:00
abstract::The Human Papillomavirus type-16 (HPV-16) E6 and E7 oncogenes are selectively retained and expressed in cervical carcinomas, and expression of E6 and E7 is sufficient to immortalize human cervical epithelial cells. Expression of the epidermal growth factor receptor (EGFR) is often increased in cervical dysplasia and c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.09.014
更新日期:2011-12-05 00:00:00
abstract::To study the possible role of immune selection in the in vivo generation of pathogenic recombinant murine leukemia viruses (MuLV), we have constructed recombinant vaccinia viruses (rVV) expressing the envelope genes of three MuLV: AKR623, MCF247, and MCF13. rVV expressing either AKR623 or MCF247 env could prime H-2b m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1370
更新日期:1994-07-01 00:00:00
abstract::Flock house virus (FHV) encodes a suppressor protein B2 to overcome antiviral RNA silencing during infection. Biochemical analyses have shown that a homodimer of B2 binds to double-stranded RNA to inhibit dicer-mediated cleavage of dsRNA and incorporation of small interfering RNAs into the RNA-induced silencing comple...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.05.023
更新日期:2012-10-25 00:00:00
abstract::The coat protein (CP) genes of all the strains of potato virus X (PVX) code for a protein of 25 kDa. Analysis of the CP by SDS-PAGE shows a migration mobility of 27 to 29 kDa, depending of the strain. Amino acid identity between some strains is too high to explain such abnormal migration by differences in primary stru...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1386
更新日期:1994-08-01 00:00:00
abstract::The genome of the sigma rhabdovirus of Drosophila melanogaster consists of six genes in the order 3' N-2-3-4-G-L 5'. The nucleotide sequences of the N and of the fourth genes were determined from cDNA clones. Each gene contained a single long open reading frame encoding polypeptides with predicted MW of 50 and 25 kDa,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1177
更新日期:1994-04-01 00:00:00
abstract::Chemokines play critical roles in HIV-1 infection, serving both to modulate viral replication and to recruit target cells to sites of infection. Interferon-gamma-inducible protein 10 (IP-10/CXCL10) is a C-X-C chemokine that acts specifically upon activated T cells and macrophages and attracts T cells into the cerebros...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00045-4
更新日期:2003-03-01 00:00:00
abstract::The regions of the AcMNPV genome represented as cytoplasmic transcripts early after infection of Spodoptera frugiperda cells prior to and encompassing the initiation of DNA replication were mapped. In vivo(32)P pulse-labeled cytoplasmic RNA from infected cells at various times postinfection was used to probe Southern ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90491-9
更新日期:1983-04-15 00:00:00
abstract::Infection of Salmonella enterica with lytic mutants of either P22 or SE1 bacteriophages triggers the expression of its DNA damage-inducible SOS response through a lexA-dependent pathway. This induction of the SOS system strictly requires the presence of the bacteriophage kil gene. Accordingly, plasmid overexpression o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.001
更新日期:2006-08-01 00:00:00
abstract::Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.09.009
更新日期:2007-03-01 00:00:00