Ceramide formation mediated by acid sphingomyelinase facilitates endosomal escape of caliciviruses.

Abstract:

:Our recent results demonstrated that bile acids facilitate virus escape from the endosomes into the cytoplasm for successful replication of porcine enteric calicivirus (PEC). We report a novel finding that bile acids can be substituted by cold treatment for endosomal escape and virus replication. This endosomal escape by cold treatment or bile acids is associated with ceramide formation by acid sphingomyelinase (ASM). ASM catalyzes hydrolysis of sphingomyelin into ceramide, which is known to destabilize lipid bilayer. Treatment of LLC-PK cells with bile acids or cold led to ceramide formation, and small molecule antagonists or siRNA of ASM blocked ceramide formation in the endosomes and significantly reduced PEC replication. Inhibition of ASM resulted in the retention of PEC, feline calicivirus or murine norovirus in the endosomes in correlation with reduced viral replication. These results suggest the importance of viral escape from the endosomes for the replication of various caliciviruses.

journal_name

Virology

journal_title

Virology

authors

Shivanna V,Kim Y,Chang KO

doi

10.1016/j.virol.2015.04.022

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

218-28

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(15)00233-0

journal_volume

483

pub_type

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