Abstract:
:In this study, we present enzymatic differences found between recombinant RTs derived from feline leukemia virus and feline immunodeficiency virus. Firstly, FIV RT showed low steady state K(m) values for dNTPs compared to FeLV RT. Consistent with this, FIV RT synthesized DNA more efficiently than FeLV RT at low dNTP concentrations. We observed similar concentration-dependent activity differences between other lentiviral (HIV-1 and SIV) and non-lentiviral (MuLV and AMV) RTs. Second, FeLV RT showed less efficient misincorporation with biased dNTP pools and mismatch primer extension capabilities, compared to FIV RT. In M13mp2 lacZalpha forward mutation assays, FeLV RT displayed approximately 11-fold higher fidelity than FIV RT. Finally, FeLV RT was less sensitive to 3TCTP and ddATP than FIV RT. This study represents the comprehensive enzymatic characterization of RTs from a lentivirus and a non-lentivirus retrovirus from the same host species. The data presented here support enzymatic divergences seen among retroviral RTs.
journal_name
Virologyjournal_title
Virologyauthors
Operario DJ,Reynolds HM,Kim Bdoi
10.1016/j.virol.2005.02.010subject
Has Abstractpub_date
2005-04-25 00:00:00pages
106-21issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(05)00112-1journal_volume
335pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.08.032
更新日期:2016-11-01 00:00:00
abstract::Commelina yellow mottle virus (CoYMV) is the type member of the badnaviruses, a genus of plant pararetroviruses. The N-terminus of the polyprotein encoded by ORF III has limited similarity to known cell-to-cell movement proteins. To test the hypothesis that the N-terminus is required for viral movement, the phenotypes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8569
更新日期:1997-06-09 00:00:00
abstract::Polyomaviral vectors are generated by transfecting 293T cells with three sets of DNAs: DNA for the expression of simian virus 40 (SV40) T antigen; DNA for the expression of SV40 capsid proteins, and vector DNA harboring a reporter gene expression cassette carrying a SV40 origin. The vector DNA harbors a minimal sequen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.032
更新日期:2008-09-15 00:00:00
abstract::We show that the product of the polarity suppression (psu) gene from bacteriophage P4 associates with P4 capsids. This association can occur when Psu is (i) provided in vivo from the P4 genome or from a plasmid or (ii) provided in vitro by mixing viable phage particles with Psu protein. Psu is unable to associate with...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90538-z
更新日期:1992-06-01 00:00:00
abstract::The complete nucleotide sequence of the nucleocapsid (N) gene of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus of plant rhabdoviruses, has been determined from cDNA clones of both the viral genomic and messenger RNAs. The previously identified N gene was adjacent to the 3' leader ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1438
更新日期:1994-08-01 00:00:00
abstract::Rice tungro bacilliform virus (RTBV) is a plant pararetrovirus and a member of the Caulimoviridae family and closely related to viruses in the Badnavirus genus. The coat protein of RTBV is part of the large polyprotein encoded by open reading frame 3 (ORF3). ORF3 of an RTBV isolate from Malaysia was sequenced (accessi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9519
更新日期:1999-01-20 00:00:00
abstract::In this study we show that macrophages (Mphi) are latently infected with murine cytomegalovirus (MCMV). After clearance of acute MCMV infection, the predominant form of chronic infection in Balb mice is latency rather than persistence. Peritoneal exudate cells (PECs) from latently infected Balb mice (3-9 months postin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8303
更新日期:1997-01-06 00:00:00
abstract::Tombusviruses are small, plus-sense, single-stranded RNA viruses of plants. RNA-dependent RNA polymerases (RdRp) of two tombusviruses, Tomato bushy stunt virus (TBSV) and Cucumber necrosis virus (CNV), have been partially purified from infected Nicotiana benthamiana plants. The obtained RdRp complexes are capable of d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0577
更新日期:2000-10-25 00:00:00
abstract::The Psu (Polarity suppression) protein of satellite bacteriophage P4 was first characterized as an anti-terminator of transcription termination in Escherichia coli. Psu is also a structural component of mature P4 capsids, where it is present as a decoration protein. Psu is located externally on the capsid surface, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1307
更新日期:1993-06-01 00:00:00
abstract::Equine influenza is caused by two serotypes of type A influenza virus, EIV-A1 and EIV-A2. The complete nucleotide sequence of the neuraminidase (NA) genes of both the A1 (N7 subtype) and A2 (N8 subtype) serotype has been determined following cloning of full-length viral NA cDNAs into pBR322. Analysis of the deduced am...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90207-2
更新日期:1986-12-01 00:00:00
abstract::We have constructed a recombinant baculovirus expressing high levels of the herpes simplex virus type 1 (HSV-1) glycoprotein E (gE) in Sf9 cells. The expressed gE migrated on gels as a double band with apparent molecular weights of 68 and 70 kDa. The recombinant gE was glycosylated based on its susceptibility to tunic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90500-o
更新日期:1992-06-01 00:00:00
abstract::Mixed infections of tobacco protoplasts with Cowpea chlorotic mottle virus (CCMV) and brome mosaic virus (BMV) have been studied. Fluorescent antibody staining showed that capsids of both viruses were made in doubly infected protoplasts but only infectious BMV could be recovered, i.e., BMV dominated in mixed infection...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90050-1
更新日期:1980-09-01 00:00:00
abstract::The 121,752-bp genome sequence of bacteriophage T5 was determined; the linear, double-stranded DNA is nicked in one of the strands and has large direct terminal repeats of 10,139 bp (8.3%) at both ends. The genome structure is consistently arranged according to its lytic life cycle. Of the 168 potential open reading f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.10.049
更新日期:2005-02-05 00:00:00
abstract::Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00348-9
更新日期:2003-09-15 00:00:00
abstract::Plant virus-encoded movement protein(s) (MP), and for many viruses the coat protein (CP), is required to mediate viral spread between plant cells via plasmodesmata (PD). Most probably, the genomic RNA of potexviruses moves through PD as assembled virions and/or as ribonucleoprotein complexes containing the CP and 25-k...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0319
更新日期:2000-06-05 00:00:00
abstract::The polyomavirus hr-t class of mutants has served as a major prototype to study the function of middle T + small T in the virus lytic cycle, Biochem. Biophys. Acta 695 (2), 69-95). The properties of these middle T + small T defective mutants were defined by comparisons with "wild-type" strains reconstructed by marker ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00030-2
更新日期:2003-03-15 00:00:00
abstract::Experiments with monoclonal antibodies raised against two laboratory strains of Sindbis virus, SB and SIN, suggested the existence of a strain-specific neutralizing antigenic site (E2-b) on the E2 glycoprotein. A comparison of monoclonal antibody binding patterns and E2 glycoprotein gene sequences of six laboratory st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90175-9
更新日期:1987-11-01 00:00:00
abstract::By employing two different buffer solutions for polyacrylamide gel electrophoresis, all genome segments of Fiji disease and maize rough dwarf virus were separated. Fiji disease virus contains ten genome segments with approximate genome molecular weights of 19.26 x 10(6) and 19.85 x 10(6), depending on the buffer emplo...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::The virion-associated Vpr protein of human immunodeficiency virus type 1 (HIV-1) alters cell cycle progression from the G2 phase, influences the virus in vivo mutation rate, and participates in the nuclear translocation of viral DNA. While many Vpr-interacting proteins have been identified, the functional relevance of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0791
更新日期:2001-03-30 00:00:00
abstract::The measles virus RNA-dependent RNA polymerase consists of two virus-encoded subunits, the phosphoprotein (P) and the large (L) protein. The P mRNA also codes for a C protein in the +1 reading frame relative to P. The activities of the measles P and C proteins from the vaccine strain, EdB, a wild-type CM strain, and a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0962
更新日期:2001-06-20 00:00:00
abstract::Previous serological studies of experimental infection with duck hepatitis B virus (DHBV) have shown that the outcome of infection depends largely on the age of the duck at the time of inoculation. To examine the hypothesis that decreased susceptibility with increased age might be due to the loss of the virus receptor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90017-j
更新日期:1992-02-01 00:00:00
abstract::The guinea pig cytomegalovirus (GPCMV) co-linear gene and potential functional homolog of HCMV UL84 (GP84) was investigated. The GP84 gene had delayed early transcription kinetics and transient expression studies of GP84 protein (pGP84) demonstrated that it targeted the nucleus and co-localized with the viral DNA poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.10.028
更新日期:2011-02-05 00:00:00
abstract::Although replication-incompetent herpes simplex virus (HSV) vectors have the capability to express foreign genes, successful development of these vectors for gene delivery would require that expression of the foreign gene be regulated. To investigate the feasibility of obtaining inducible expression of a foreign gene ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0635
更新日期:1996-12-01 00:00:00
abstract::Evidence is presented for the glycosylation of the cauliflower mosaic virus (CaMV) capsid polypeptide. Fluorescent derivatives of concanavalin A (Con A) were shown to bind specifically to the dissociated CaMV capsid polypeptides in polyacrylamide gels. Con A also bound specifically to intact CaMV, so inhibiting the at...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90510-9
更新日期:1981-03-01 00:00:00
abstract::Primary strains of human immunodeficiency virus type 1 (HIV-1) are known to adapt to replication in cell lines in vitro by becoming sensitive to soluble CD4 (sCD4) and neutralizing antibodies (NAb). T-cell lines favor isolation of variants that use CXCR4 as a co-receptor, while primary isolates predominantly use CCR5....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.012
更新日期:2004-03-30 00:00:00
abstract::Immunodeficiency caused by HIV infection probably results from profound dysregulation of normal T lymphocyte properties by the virus. Despite description of the virus cytopathicity and numerous modifications in T cell functions, such as perturbation of antigen receptor signaling, CD4 downregulation, and induction of a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1124
更新日期:1994-03-01 00:00:00
abstract::KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.10.013
更新日期:2014-01-05 00:00:00
abstract::We reported previously that carbohydrate attachment to an overlapping glycosylation site adjacent to the signal peptide of GP3 from equine arteritis virus (EAV) prevents cleavage. Here we investigated whether this unusual processing scheme is a feature of GP3s of other Arteriviridae, which all contain a glycosylation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.11.026
更新日期:2018-04-01 00:00:00
abstract::A natural variation is observed at position 4 of the 3'-end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.09.022
更新日期:2010-12-20 00:00:00
abstract::Animal viruses profoundly modify the metabolism of lipids, the synthesis of new membranes, and membrane traffic. These alterations are related to the replication of viral genomes. Addition of oleic acid from the beginning of poliovirus infection inhibits the appearance of virus polypeptides at concentrations that do n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90802-i
更新日期:1991-11-01 00:00:00