Abstract:
:The complete nucleotide sequence of the nucleocapsid (N) gene of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus of plant rhabdoviruses, has been determined from cDNA clones of both the viral genomic and messenger RNAs. The previously identified N gene was adjacent to the 3' leader RNA and began at position 91 from the 3' end of the LNYV genome. Analysis of the sequence showed that the N mRNA contained a 78-nt 5' untranslated region, followed by a 1377-nt open reading frame (ORF) encoding a 459-amino-acid protein. This ORF was similar in size to the N protein ORFs of other rhabdoviruses. However, little if any direct sequence homology was found with N protein sequences of other rhabdoviruses. Short amino acid sequences were found to be conserved between the N proteins of LNYV and sonchus yellow net virus (SYNV), a member of the genus Nucleorhabdovirus of plant rhabdoviruses. Among them, a sequence of 24 amino acids which was similar between LNYV and SYNV N proteins corresponded to a region with high homology between isolates of vesicular stomatitis virus and rabies virus N protein sequences, the type members of the genera Vesiculovirus and Lyssavirus of animal rhabdoviruses, respectively.
journal_name
Virologyjournal_title
Virologyauthors
Wetzel T,Dietzgen RG,Geering AD,Dale JLdoi
10.1006/viro.1994.1438subject
Has Abstractpub_date
1994-08-01 00:00:00pages
1054-7issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71438-3journal_volume
202pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::A human cytomegalovirus (HCMV)-induced polypeptide of 68,000 Da (p68) with protein kinase activity was identified using a monoclonal antibody (F6b) produced against HCMV-infected cell proteins. p68 was detected by immunoprecipitation from 3 to 120 hr after infection and was induced by several strains of human and not ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90295-2
更新日期:1984-04-30 00:00:00
abstract::The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90695-l
更新日期:1992-07-01 00:00:00
abstract::Dengue virus (DENV) and West Nile virus (WNV) are members of the Flavivirus genus of positive-strand RNA viruses. RNA sequences and structures, primarily in the untranslated regions, have been shown to modulate flaviviral gene expression and genome replication. Previously, we demonstrated that a structure in the DENV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.034
更新日期:2008-09-30 00:00:00
abstract::The envelope glycoprotein (Env) of Moloney murine leukemia virus (MoMuLV) is proteolytically processed and transported to the cell surface where it can be incorporated into budding virions. Cell surface Env is frequently detected using an indirect immunofluorescence assay and fluorescence-activated cell sorting (FACS)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9073
更新日期:1998-04-10 00:00:00
abstract::To study the role of human papillomavirus (HPV) infection in the development of genetic instability, we transduced normal human airway and anogenital epithelial cells with various combinations of HPV-16 E6, E7, and the reverse transcriptase component of telomerase (hTERT). Cell lines generated by co-expression of E7 w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.06.027
更新日期:2005-09-30 00:00:00
abstract::The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode on...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90344-7
更新日期:1985-04-30 00:00:00
abstract::Particles purified from the liver of hepatitis B virus-infected patients were previously shown by us to incorporate 32P-deoxynucleotides into viral DNA and DNA-RNA hybrid molecules when incubated in a DNA polymerase reaction mixture. In this investigation, similar particles from duck and ground squirrel livers infecte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90330-1
更新日期:1984-11-01 00:00:00
abstract::Nucleotide sequence determination of the coat protein cistron of the alfalfa mosaic virus (AIMV) temperature-sensitive mutant, Tbts 7 (uv) revealed a small number of point mutations of which only one results in the replacement of an amino acid: the asparagine residue at position 126 is replaced by an aspartate residue...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90609-0
更新日期:1989-08-01 00:00:00
abstract::The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 X 10(6). The four species obse...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90406-9
更新日期:1984-03-01 00:00:00
abstract::Equine influenza is caused by two serotypes of type A influenza virus, EIV-A1 and EIV-A2. The complete nucleotide sequence of the neuraminidase (NA) genes of both the A1 (N7 subtype) and A2 (N8 subtype) serotype has been determined following cloning of full-length viral NA cDNAs into pBR322. Analysis of the deduced am...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90207-2
更新日期:1986-12-01 00:00:00
abstract::Marburg virus (MBGV), for which no vaccines or treatments currently exist, causes an acute hemorrhagic fever with a high mortality rate in humans. We previously showed that immunization with either killed MBGV or a glycoprotein (GP) subunit prevented lethal infection in guinea pigs. In the studies reported here, an RN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9367
更新日期:1998-11-10 00:00:00
abstract::We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8944
更新日期:1998-01-20 00:00:00
abstract::An immunodominant region of VP3, one of the two structural proteins of infectious bursal disease virus (IBDV strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in Escherichia coli, followed by immunoblot analysis of the synthesized products. The epitope located within 58 amin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90457-m
更新日期:1991-10-01 00:00:00
abstract::There is growing evidence that the receptor-binding characteristics of influenza viruses are affected by the host-dependent glycosylation of viral hemagglutinin (HA). To better understand these effects, we propagated two variants of the human influenza virus USSR/90/77 (which differed by the mutation Asn131 reversible...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9224
更新日期:1998-08-01 00:00:00
abstract::The baculovirus-insect cell system is used routinely for foreign glycoprotein production, but the precise nature of the N-glycosylation pathway in this system remains unclear. Some studies indicate that these cells cannot process N-linked oligosaccharides to complex forms containing outer-chain galactose and sialic ac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1508
更新日期:1995-10-01 00:00:00
abstract::The role of oxygen tension level is a well-known phenomenon that has been studied in oncology and radiotherapy since about 60 years. Oxygen tension may inhibit or stimulate propagation of viruses in vitro as well as in vivo. In turn modulating oxygen metabolism may constitute a novel approach to treat viral infections...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2013.06.018
更新日期:2013-09-01 00:00:00
abstract::Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90220-4
更新日期:1984-09-01 00:00:00
abstract::HK97 is a temperate dsDNA bacteriophage of Escherichia coli that is structurally similar to phage lambda, with an icosahedral head of triangulation (7) number 7. Although the capsids of several large dsDNA phages have been studied extensively using a variety of biophysical approaches, no high-resolution structure is a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9040
更新日期:1998-03-30 00:00:00
abstract::Multiple nucleoside resistance involves specific mutational patterns of the HIV-1 pol gene that are independent of the classic mutations conferring resistance to individual dideoxynucleosides. These include a cluster of five mutations in the reverse-transcriptase (RT) coding region (A62V, V75I, F77L, F116Y, and Q151M)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0261
更新日期:2000-05-10 00:00:00
abstract::The amino acid sequence of the fiber from adenovirus type 5 has been deduced from the nucleotide sequence of the fiber gene. This sequence is compared with that of adenovirus type 2, a closely related serotype. We find 69% homology for the fiber protein whereas the known nonstructural proteins of these two serotypes h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90150-4
更新日期:1987-12-01 00:00:00
abstract::Varicella Zoster virus (VZV) productively infects humans causing varicella upon primary infection and herpes zoster upon reactivation from latency in neurons. In vitro studies using cell-associated VZV infection have demonstrated productive VZV-infection, while a few recent studies of human neurons derived from stem c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.05.021
更新日期:2013-09-01 00:00:00
abstract::Hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family Bunyaviridae. Nucleocapsid RNA from Hantaan virions and a control bunyavirus were examined for ribonuclease A (RNase A) se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90514-7
更新日期:1983-12-01 00:00:00
abstract::Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, heparin) and copolymers of acrylic acid with vinylalcohol sulfate have proved to be potent inhibitors of human cytomegalovirus (CMV) infectivity in vitro. Sulfated alpha-cyclodextrins are only weak inhibitors of CMV. A close correlation was found...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90680-n
更新日期:1992-07-01 00:00:00
abstract::To better understand the origin of human T-cell leukemia virus type l (HTLV-l) in South America, we conducted a phylogenetic study on 27 new HTLV-ls in Brazil. These were obtained from Brazilians of various ethnic origins, such as Japanese immigrants, whites, blacks and mulattos. We amplified and sequenced proviral DN...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1999-08-15 00:00:00
abstract::Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90199-2
更新日期:1989-10-01 00:00:00
abstract::The genomic S RNA segment of Prospect Hill virus (PHV), a member of the Hantavirus genus, was molecularly cloned and the nucleotide sequence of the cDNA determined. The PHV S RNA segment is 1675 nucleotides long. A long open reading frame was identified in the viral complementary-sense RNA that could encode a 433 amin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90197-y
更新日期:1990-03-01 00:00:00
abstract::The uncoating of tobacco mosaic virus (TMV) particles in protoplasts isolated from leaves of transgenic tobacco plants that express the TMV coat protein gene was investigated. Extracts of these protoplasts collected up to 1 hr after inoculation with TMV contained fewer of the complexes ("striposomes") thought to be in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90163-l
更新日期:1990-12-01 00:00:00
abstract::It is likely that phylogroup 2 lyssaviruses circulate within bat reservoirs. We adapted a pseudotype (pt) neutralisation assay (PNA) to a multiplex format enabling serosurveillance for Lagos bat virus (LBV), Mokola virus (MOKV) and West Caucasian bat virus (WCBV) in a potential reservoir, the African straw-coloured fr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.09.014
更新日期:2010-12-20 00:00:00
abstract::In this study, we show that adenoviral infection induced accumulation of the sphingolipid ceramide in a dose- and time-dependent manner. This accumulation preceded cell lysis, occurred in the absence of biochemical evidence of apoptosis, and was derived from de novo synthesis of ceramide. An adenovirus mutant that lac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.09.060
更新日期:2006-02-05 00:00:00
abstract::During respiratory syncytial virus (RSV) infection there is a close physical interaction between the filamentous actin (F-actin) and the virus, involving both inclusion bodies and the virus filaments. This interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.007
更新日期:2007-12-20 00:00:00