Abstract:
:To study the role of human papillomavirus (HPV) infection in the development of genetic instability, we transduced normal human airway and anogenital epithelial cells with various combinations of HPV-16 E6, E7, and the reverse transcriptase component of telomerase (hTERT). Cell lines generated by co-expression of E7 with E6 and/or hTERT (i.e., E6/E7, E7/hTERT, and E6/E7/hTERT) exhibited extra copies of chromosome 20 and specific amplification of the 20q12-ter region, whereas those generated without E7 (i.e., hTERT alone or E6/hTERT) did not. Co-expression of hTERT and a dominant-negative version of cdk4 that has been shown to inactivate the retinoblastoma (pRb) pathway also resulted in 20q amplification. Interestingly, extra copies of chromosome 20 were observed in early passage keratinocytes that expressed E7 alone, and microarray expression analysis revealed that genes in the 20q region and on chromosome 5 were specifically upregulated in these cells. Our results indicate that chromosome 20q amplification is an early event that may be specifically caused by expression of E7 through inactivation of the pRb pathway in human epithelial cells.
journal_name
Virologyjournal_title
Virologyauthors
Klingelhutz AJ,Qian Q,Phillips SL,Gourronc FA,Darbro BW,Patil SRdoi
10.1016/j.virol.2005.06.027subject
Has Abstractpub_date
2005-09-30 00:00:00pages
237-44issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(05)00368-5journal_volume
340pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The prevalence of hepatitis B virus vaccine escape mutants has increased as a consequence of the introduction of global vaccination programs. Furthermore and as a consequence of the organization of the genome of hepatitis B virus (HBV) into overlapping reading frames, the selection of polymerase mutants during long-te...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1246
更新日期:2002-02-15 00:00:00
abstract::Geographical separation of host species has shaped the avian influenza A virus gene pool into independently evolving Eurasian and American lineages, although phylogenetic evidence for gene flow and reassortment indicates that these lineages also mix on occasion. While the evolutionary dynamics of the avian influenza g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.002
更新日期:2009-08-01 00:00:00
abstract::The sid (size determination) gene product of phage P4 is known to be involved in capsid-size determination. Moreover, the capsid-size determination function interferes with the lytic development of its helper P2, presumably because the helper genome is too large to be packaged into P4-size capsids. In order to study P...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0270
更新日期:1996-05-15 00:00:00
abstract::To study the process of membrane fusion in Morbilliviruses, the fusion (F) glycoproteins of Peste des petits ruminants virus (PPRV) and Rinderpest virus (RPV) were expressed transiently in mammalian cells. The recombinant F proteins were found to be localized at the surface of transfected cells. The fusion activity, a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1120
更新日期:2001-10-10 00:00:00
abstract::The role of anti-viral antibodies in homologous protective immunity to a virulent African swine fever virus (ASFV) strain E75 was examined by passive transfer experiments in swine. Eighty-five percent of animals (n = 14) that received anti-ASFV immunoglobulin (Ig) survived challenge infection, while 100% mortality was...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1040
更新日期:1994-01-01 00:00:00
abstract::Methods currently used for HIV-1 viral load measurements are very sensitive, but cannot distinguish between infectious and noninfectious particles. Here we describe the development of a novel, sensitive, and highly reproducible method that allows rapid isolation and quantification of infectious particles from patient ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.05.022
更新日期:2004-08-15 00:00:00
abstract::Synthesis of unintegrated retroviral DNA via reverse transcription is thought to involve two separate template switches. Minus-strand strong-stop DNA, a major product of in vitro reactions using detergent-treated virions, is synthesized prior to the first template switch. Plus-strand strong-stop DNA, which is found ba...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1237
更新日期:1993-05-01 00:00:00
abstract::Sequences critical for the activity of the measles virus (MV) RNA polymerase in transcription and replication were analyzed using a MV genomic cDNA library containing overlapping clones encompassing the entire MV genome. Clones corresponding to the 3' and 5' ends of the MV genome were identified and sequenced, and the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90564-8
更新日期:1988-06-01 00:00:00
abstract::The N-terminal domains of the NS1 protein of influenza B virus (NS1B protein) and the NS1 protein of influenza A virus (NS1A protein) share one function: binding double-stranded RNA (dsRNA). Here we show that the N-terminal domain of the NS1B protein possesses an additional function that is not shared by its NS1A coun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1663
更新日期:2002-12-20 00:00:00
abstract::HPV-16 is the major causes of cervical cancer. Persistence of infection is a necessary event for progression of the infection to cancer. Among other factors, virus persistence is due the viral proteins fighting the immune response. HPV-16 E5 down-regulates MHC/HLA class I, which is much reduced on the cell surface and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.07.044
更新日期:2010-11-10 00:00:00
abstract::To clarify the causal relationship between human papillomavirus (HPV) and cervical intraepithelial neoplasia (CIN), we analyzed 386 unfixed biopsy specimens by blot hybridization at Tm -40 degrees C, targeting 38 skin and 42 genital HPVs. By the recognition of PstI, BanI, and MspI cleavage patterns, single genital, bu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0865
更新日期:2001-04-25 00:00:00
abstract::A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35S promoter in an agroinfection vector. The biological activity o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.9945
更新日期:1995-12-01 00:00:00
abstract::The primary cell types that sustain polyomavirus (Py) replication following intranasal infection as well as the nature of the host cellular response to Py were unknown. As this is an essential and specific site for virus entry, it seems likely that viral gene function must be adapted to these mucosal tissues. Using im...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0030
更新日期:2000-01-05 00:00:00
abstract::Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5 degrees. I...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90139-3
更新日期:1983-10-15 00:00:00
abstract::The human T cell leukemia virus type I (HTLV-I) is an oncogenic retrovirus that is etiologically linked to the genesis of adult T cell leukemia (ATL) as well as HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Emerging evidence suggests that the pathogenicity of HTLV-I involves deregulated activati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.12.008
更新日期:2005-03-01 00:00:00
abstract::Among 489 bats of 11 species in China, three novel paramyxoviruses [Tuhokovirus 1, 2 and 3 (ThkPV-1, ThkPV-2 and ThkPV-3)] were discovered in 15 Leschenault's rousettes. Phylogenetically, the three viruses are most closely related to Menangle and Tioman virus. Genome analysis showed that their 3'-leader sequences are ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.049
更新日期:2010-08-15 00:00:00
abstract::A mouse model system for a lethal encephalitis due to influenza has been established by stereotaxic microinjection with the recombinant R404BP strain of influenza A virus into the olfactory bulb of C57BL/6 mice. The virus infection spread selectively to neurons in nuclei of the broad areas of the brain parenchyma that...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1601
更新日期:2002-11-25 00:00:00
abstract::In a plantar cyst composed of the wall of the squamous cell layer and the horny inner substance in the lower dermis, we found characteristic pathological changes, such as cytoplasmic eosinophilic inclusions and vacuolated structure, and, immunohistochemically, the papillomavirus capsid antigen. The human papillomaviru...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91254-r
更新日期:1992-09-01 00:00:00
abstract::ICP27 is an essential herpes simplex virus type 1 (HSV-1) nuclear protein which regulates viral early and late genes during infection. The exact mechanism by which ICP27 modulates viral gene expression is unknown, but considerable evidence suggests that it functions posttranscriptionally. In this study, we have asked ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.9006
更新日期:1998-03-01 00:00:00
abstract::We cloned the complete complementary DNA of an isolate of the hepatitis C virus, HCV-S1, into a tetracycline-inducible expression vector and stably transfected it into two human hepatoma cell lines, Huh7 and HepG2. Twenty-six Huh7 and two HepG2-positive clones were obtained after preliminary screening. Two Huh7 (SH-7 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1687
更新日期:2002-11-10 00:00:00
abstract::Unusual Plum pox virus (PPV) isolates (named Tat isolates) were discovered on sour cherry (Prunus cerasus) in Russia. They failed to be recognized by RT-PCR using commonly employed primers specific to the strains C or CR (the only ones that proved able to infect sour cherry) as well as to the strains M and W. Some of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.12.016
更新日期:2017-02-01 00:00:00
abstract::Re-emergence of ZIKV has caused infections in more than 1.5 million people. The molecular mechanism and pathogenesis of ZIKV is not well explored due to unavailability of adequate model and lack of publically accessible resources to provide information of ZIKV-Human protein interactome map till today. This study made ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.11.007
更新日期:2018-01-15 00:00:00
abstract::Viruses are infectious particles whose viability is dependent on the cells of living organisms, such as bacteria, plants, and animals. It is of great interest to discover how viruses function inside host cells in order to develop therapies to treat virally infected organisms. The fruit fly Drosophila melanogaster is a...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2011.11.016
更新日期:2012-02-05 00:00:00
abstract::Therapeutic immunization of HIV-1-infected individuals may induce and/or enhance HIV-1-specific immune responses and decrease the dependency on antiretroviral drug treatment. However, repeated immunizations with live-recombinant vectors may induce vector-specific immune responses that interfere with the elicitation of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1722
更新日期:2002-12-05 00:00:00
abstract::West Nile virus (WNV) has become firmly established in northeastern US, reemerging every summer since its introduction into North America in 1999. To determine whether WNV overwinters locally or is reseeded annually, we examined the patterns of viral lineage persistence and replacement in Connecticut over 10 consecuti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.06.006
更新日期:2011-08-15 00:00:00
abstract::The HIV-1 protein Vpr enhances macrophage infection, triggers G2 cell cycle arrest, and targets cells for NK-cell killing. Vpr acts through the CRL4(DCAF1) ubiquitin ligase complex to cause G2 arrest and trigger expression of NK ligands. Corresponding ubiquitination targets have not been identified. UNG2 and SMUG1 are...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.06.010
更新日期:2013-09-01 00:00:00
abstract::Long-term persistent infections were established with the flavivirus, West Nile virus (WNV), strain E101, in embryofibroblast cultures derived from susceptible C3H/HE and congenic-resistant C3H/RV mice. Cultures were initially maintained by weekly subculture at 37 degrees, but at passage 6 sister cultures were shifted...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90454-4
更新日期:1985-01-15 00:00:00
abstract::Segment reassortment of negative strand viruses is an important mechanism for the development of new virus strains with altered pathogenicity. This study reports on in vitro generation of reassortants between Andes (ANDV) and Sin Nombre (SNV) viruses. Although they both cause hantavirus pulmonary syndrome (HPS), ANDV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.012
更新日期:2004-10-01 00:00:00
abstract::The NIa protein of potyviruses provides VPg and proteolytic functions during virus replication. It has also been shown to confer host genotype-specific movement functions in plants. Specifically, NIa from tobacco etch virus (TEV)-Oxnard, but not from most other strains, confers the ability to move long distances in Ni...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0416
更新日期:2000-08-01 00:00:00
abstract::Turnip crinkle carmovirus (TCV) has a uniquely high recombination frequency and nonrandom cross-over site distribution among the recombining TCV-associated satellite RNAs. An in vitro system has been developed that includes a partially purified TCV replicase preparation (RdRp) and chimeric RNAs that resemble the putat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9342
更新日期:1998-09-30 00:00:00