Abstract:
:Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37 degrees. We have identified a fraction of infecting virus which binds tightly to cells at 4 degrees, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37 degrees appears slow, with uptake occurring over a period of at least 16 hr.
journal_name
Virologyjournal_title
Virologyauthors
Pugh JC,Summers JWdoi
10.1016/0042-6822(89)90199-2subject
Has Abstractpub_date
1989-10-01 00:00:00pages
564-72issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(89)90199-2journal_volume
172pub_type
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