Lipid-raft-dependent Coxsackievirus B4 internalization and rapid targeting to the Golgi.


:Coxsackievirus B4 (CBV4), a member of the Picornavirus genus, has long been implicated in the development of insulin-dependent diabetes mellitus (IDDM), by viral-induced pancreatic cell damage. Although the pancreotropic nature of this virus is well documented, the early stages of CBV4 viral infection that involve the attachment of virions to the cell surface by binding to their cellular receptors followed by entry into the cell, are poorly understood. In this study, we show that the entry of CBV4 requires functional lipid rafts as the site of virus attack. In addition, we show that this virus is endocytosed independently of clathrin-associated machinery and is delivered to the Golgi via a lipid-raft-dependent mechanism.






Triantafilou K,Triantafilou M




Has Abstract


2004-08-15 00:00:00
















  • The latency-associated nuclear antigen tethers the Kaposi's sarcoma-associated herpesvirus genome to host chromosomes in body cavity-based lymphoma cells.

    abstract::Viruses that establish latent infection must maintain their DNA in the host nucleus through many cellular generations. Here we identify a novel mechanism by which the gammaherpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) may achieve this persistence in latently infected body cavity-based lymphoma (BCBL) cell...


    pub_type: 杂志文章


    authors: Cotter MA 2nd,Robertson ES

    更新日期:1999-11-25 00:00:00

  • Amplification of the chromosome 20q region is associated with expression of HPV-16 E7 in human airway and anogenital epithelial cells.

    abstract::To study the role of human papillomavirus (HPV) infection in the development of genetic instability, we transduced normal human airway and anogenital epithelial cells with various combinations of HPV-16 E6, E7, and the reverse transcriptase component of telomerase (hTERT). Cell lines generated by co-expression of E7 w...


    pub_type: 杂志文章


    authors: Klingelhutz AJ,Qian Q,Phillips SL,Gourronc FA,Darbro BW,Patil SR

    更新日期:2005-09-30 00:00:00

  • Evidence for spontaneous circle formation in the replication of the satellite RNA of tobacco ringspot virus.

    abstract::Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...


    pub_type: 杂志文章


    authors: Van Tol H,Buzayan JM,Bruening G

    更新日期:1991-01-01 00:00:00

  • Binding to CD4 of synthetic peptides patterned on the principal neutralizing domain of the HIV-1 envelope protein.

    abstract::The interaction between the viral envelope protein gp120 and the cellular surface antigen CD4 is a key event in HIV-1 infection. Reciprocal high affinity binding sites have been located in the first domain of CD4 and in the carboxy-terminal region of gp120, respectively. Upon infection, the membranes of the target cel...


    pub_type: 杂志文章


    authors: Autiero M,Abrescia P,Dettin M,Di Bello C,Guardiola J

    更新日期:1991-12-01 00:00:00

  • Deletion of glycoprotein gE reduces the propagation of pseudorabies virus in the nervous system of mice after intranasal inoculation.

    abstract::A pseudorabies virus (PrV) mutant, deficient in the nonessential glycoprotein E (gE) and expressing the LacZ gene (gE- beta gal+ PrV), and its rescued virus were inoculated intranasally in mice. The median lethal dose of gE- beta gal+ PrV was similar to that of the parental Kaplan strain, but mice survived longer and ...


    pub_type: 杂志文章


    authors: Babic N,Klupp B,Brack A,Mettenleiter TC,Ugolini G,Flamand A

    更新日期:1996-05-01 00:00:00

  • An analysis of the requirements for human cytomegalovirus oriLyt-dependent DNA synthesis in the presence of the herpes simplex virus type 1 replication fork proteins.

    abstract::Activation of the human cytomegalovirus (HCMV) origin of replication (oriLyt) was previously demonstrated in transient transfection assays in permissive human fetal fibroblasts and nonpermissive Vero cells, and shown to require six viral proteins that function at the replication fork plus a number of HCMV products tha...


    pub_type: 杂志文章


    authors: Reid GG,Ellsmore V,Stow ND

    更新日期:2003-04-10 00:00:00

  • Epidermal growth factor receptor is a co-factor for transmissible gastroenteritis virus entry.

    abstract::Transmissible gastroenteritis virus (TGEV) causes severe diarrhea and high mortality in newborn piglets. It is well established that porcine intestinal epithelium is the target of the TGEV infection, however the mechanism that TGEV invades the host epithelium remains largely unknown. Aminopeptidase N (APN) is a known ...


    pub_type: 杂志文章


    authors: Hu W,Zhang S,Shen Y,Yang Q

    更新日期:2018-08-01 00:00:00

  • Structural features of envelope proteins on hepatitis C virus-like particles as determined by anti-envelope monoclonal antibodies and CD81 binding.

    abstract::The envelope glycoprotein E2 of hepatitis C virus (HCV) is a major component of the viral envelope. Knowledge of its topologic features and antigenic determinants in virions is crucial in understanding the viral binding sites to cellular receptor(s) and the induction of neutralizing antibodies. The lack of a robust ce...


    pub_type: 杂志文章


    authors: Triyatni M,Vergalla J,Davis AR,Hadlock KG,Foung SK,Liang TJ

    更新日期:2002-06-20 00:00:00

  • Walleye dermal sarcoma virus rv-cyclin inhibits NF-kappaB-dependent transcription.

    abstract::The retroviral cyclin protein (rv-cyclin) of walleye dermal sarcoma virus contains two known functional domains, a cyclin box motif and a carboxy terminal transcription activation domain (AD). The AD contacts TATA-binding protein-associated factor 9 (TAF9), and this action is necessary for both positive and negative r...


    pub_type: 杂志文章


    authors: Quackenbush SL,Linton A,Brewster CD,Rovnak J

    更新日期:2009-03-30 00:00:00

  • Molecular characterisation of a novel pathogenic avipoxvirus from the Australian magpie (Gymnorhina tibicen).

    abstract::Avipoxviruses are significant pathogens infecting a wide range of wild and domestic bird species globally. Here, we describe a novel genome sequence of magpiepox virus (MPPV) isolated from an Australian magpie. In the present study, histopathologically confirmed cutaneous pox lesions were used for transmission electro...


    pub_type: 杂志文章


    authors: Sarker S,Batinovic S,Talukder S,Das S,Park F,Petrovski S,Forwood JK,Helbig KJ,Raidal SR

    更新日期:2020-01-15 00:00:00

  • Characterization of a nonhemagglutinating mutant of canine parvovirus.

    abstract::A nonhemagglutinating mutant of a 1978 isolate of canine parvovirus (CPV) was derived after repeated passages in the NLFK feline kidney cell line. The mutant CPV was antigenically indistinguishable from wild-type virus when tested with 82 monoclonal antibodies, and it replicated in cat and dog cell lines in culture. S...


    pub_type: 杂志文章


    authors: Parrish CR,Burtonboy G,Carmichael LE

    更新日期:1988-03-01 00:00:00

  • Interferon-inducible MyD88 protein inhibits hepatitis B virus replication.

    abstract::Myeloid differential primary response protein (MyD88) is a critical component in the signaling cascade through Toll-like receptors (TLRs) and is induced by alpha interferon (IFN-alpha). To examine the role of MyD88 in the antiviral activity of IFN-alpha against hepatitis B virus (HBV), we established MyD88 stably expr...


    pub_type: 杂志文章


    authors: Xiong W,Wang X,Liu X,Xiang L,Zheng L,Yuan Z

    更新日期:2004-02-20 00:00:00

  • Infection of bovine dendritic cells by rinderpest or measles viruses induces different changes in host transcription.

    abstract::The morbilliviruses are a closely related genus which are very similar in their sequences and share a common receptor, but nevertheless show significant restriction in the host species in which they cause disease. One contribution to this restriction might be the nature of the hosts' responses to infection. We have us...


    pub_type: 杂志文章


    authors: Nanda SK,Baron J,Royall E,Robinson L,Falciani F,Baron MD

    更新日期:2009-12-20 00:00:00

  • Comparison of four regions in the replicase gene of heterologous infectious bronchitis virus strains.

    abstract::Infectious bronchitis virus (IBV) produces six subgenomic (sg) mRNAs, each containing a 64 nucleotide (nt) leader sequence, derived from the 5' end of the genome by a discontinuous process. Several putative functional domains such as a papain-like proteinase (PL(pro)), main protease (M(pro)), RNA-dependent RNA polymer...


    pub_type: 杂志文章


    authors: Mondal SP,Cardona CJ

    更新日期:2004-06-20 00:00:00

  • Adeno-associated virus infection of murine fibroblasts with help provided by mouse adenovirus.

    abstract::Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant p...


    pub_type: 杂志文章


    authors: Bhrigu V,Trempe JP

    更新日期:2009-07-20 00:00:00

  • HPV8 activates cellular gene expression mainly through Sp1/3 binding sites.

    abstract::The human papillomavirus type 8 (HPV8) is associated with skin cancer development. The goal of this study was to investigate the effects of HPV8 oncoproteins on cellular gene expression and the identification of key regulators. We performed affymetrix microarray analyses to identify differentially expressed genes and ...


    pub_type: 杂志文章


    authors: Kirschberg M,Heuser S,Syed AS,Steger G,Majewski S,Hufbauer M,Akgül B

    更新日期:2019-09-01 00:00:00

  • Population dynamics studies of wild-type and drug-resistant mutant HIV in mixed infections.

    abstract::We have studied the population dynamics in response to selective drug pressure of mixtures of wild-type and mutant HIV viruses exposed to either an inhibitor of the viral protease or a nonnucleoside allosteric inhibitor of the viral reverse transcriptase. In order to quantitate mutant virus present in a mixed populati...


    pub_type: 杂志文章


    authors: Rayner MM,Cordova B,Jackson DA

    更新日期:1997-09-15 00:00:00

  • Altered properties of the herpes simplex virus ICP8 DNA-binding protein in cells infected with ICP27 mutant viruses.

    abstract::Herpes simplex virus mutant strains that fail to encode a functional ICP27 polypeptide show a reduction in their ability to replicate viral DNA compared to wild-type virus. To define the nature of this defect, we examined the formation of viral DNA replication structures in the nucleus of cells infected with these mut...


    pub_type: 杂志文章


    authors: Curtin KD,Knipe DM

    更新日期:1993-09-01 00:00:00

  • Molecular cloning of a feline leukemia provirus integrated adjacent to the c-myc gene in a feline T-cell leukemia cell line and the unique structure of its long terminal repeat.

    abstract::This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DN...


    pub_type: 杂志文章


    authors: Miura T,Shibuya M,Tsujimoto H,Fukasawa M,Hayami M

    更新日期:1989-04-01 00:00:00

  • Closterovirus encoded HSP70 homolog and p61 in addition to both coat proteins function in efficient virion assembly.

    abstract::Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...


    pub_type: 杂志文章


    authors: Satyanarayana T,Gowda S,Mawassi M,Albiach-Martí MR,Ayllón MA,Robertson C,Garnsey SM,Dawson WO

    更新日期:2000-12-05 00:00:00

  • Destabilization of the RB tumor suppressor protein and stabilization of p53 contribute to HPV type 16 E7-induced apoptosis.

    abstract::Cells that express the human papillomavirus (HPV) type 16 E7 oncoprotein are predisposed to undergo apoptosis. Transgenic mice that have E7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. We established a cell cu...


    pub_type: 杂志文章


    authors: Jones DL,Thompson DA,Münger K

    更新日期:1997-12-08 00:00:00

  • A non-consensus branch point plays an important role in determining the stability of the 2-kb LAT intron during acute and latent infections of herpes simplex virus type-1.

    abstract::Herpes simplex virus type 1 (HSV-1) establishes lifelong latent infection in sensory neurons of the peripheral nervous system. During HSV latency, the latency-associated transcripts (LATs) are the only viral transcripts abundantly expressed. The most abundant form of LATs is a 2-kb stable intron spliced from a primary...


    pub_type: 杂志文章


    authors: Mukerjee R,Kang W,Suri V,Fraser NW

    更新日期:2004-07-01 00:00:00

  • Properties of a new virus belonging to nodaviridae found in larval striped jack (Pseudocaranx dentex) with nervous necrosis.

    abstract::Spherical virus particles were purified from larval striped jack (Pseudocaranx dentex) with nervous necrosis. The virus consists of nonenveloped particles, about 25 nm in diameter, and contains two single-stranded, positive-sense RNA molecules with molecular weights of 1.01 x 10(6)Da (RNA 1) and 0.49 x 10(6)Da (RNA 2)...


    pub_type: 杂志文章


    authors: Mori K,Nakai T,Muroga K,Arimoto M,Mushiake K,Furusawa I

    更新日期:1992-03-01 00:00:00

  • Promoter, spliced leader, and coding sequence for BICP4, the largest of the immediate-early proteins of bovine herpesvirus 1.

    abstract::We report the complete nucleotide sequence of the bovine herpesvirus 1 (BHV-1) immediate-early gene encoding BICP4, the homolog of the ICP4 protein of herpes simplex virus. Combined with previous mapping studies, the sequence analysis revealed that the transcript for BICP4 consisted of a noncoding leader RNA (exon 1; ...


    pub_type: 杂志文章


    authors: Schwyzer M,Vlcek C,Menekse O,Fraefel C,Paces V

    更新日期:1993-11-01 00:00:00

  • Ross River virus variants selected during passage in chick embryo fibroblasts: serological, genetic, and biological changes.

    abstract::Serial passage of Ross River virus in chick embryo fibroblasts selected for virus variants altered in their reactions to neutralizing monoclonal antibodies. The E1 and E2 genes of each antigenic variant were sequenced; single nucleotide changes were found in E2 leading to amino acid substitutions at either residue 4 (...


    pub_type: 杂志文章


    authors: Kerr PJ,Weir RC,Dalgarno L

    更新日期:1993-03-01 00:00:00

  • The nonstructural protein 8 (nsp8) of the SARS coronavirus interacts with its ORF6 accessory protein.

    abstract::Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The SARS-CoV genome is predicted to contain 14 functional open reading frames (ORFs). The first ORF (1a and 1b) encodes a large polyprotein that is cleaved into nonstructural proteins (nsp)...


    pub_type: 杂志文章


    authors: Kumar P,Gunalan V,Liu B,Chow VT,Druce J,Birch C,Catton M,Fielding BC,Tan YJ,Lal SK

    更新日期:2007-09-30 00:00:00

  • Bipartite geminivirus host adaptation determined cooperatively by coding and noncoding sequences of the genome.

    abstract::Bipartite geminiviruses are small, plant-infecting viruses with genomes composed of circular, single-stranded DNA molecules, designated A and B. Although they are closely related genetically, individual bipartite geminiviruses frequently exhibit host-specific adaptation. Two such viruses are bean golden mosaic virus (...


    pub_type: 杂志文章


    authors: Petty IT,Carter SC,Morra MR,Jeffrey JL,Olivey HE

    更新日期:2000-11-25 00:00:00

  • Delineation of regions important for heteromeric association of hepatitis C virus E1 and E2.

    abstract::Hepatitis C virus (HCV) is the major causative agent of blood-borne non-A non-B hepatitis. The persistence of HCV infection is believed to reflect escape from the host immunosurveillance system by mutations in hypervariable region 1 (HVR1) of the envelope protein 2 (E2). Two envelope proteins of HCV, E1 and E2, have b...


    pub_type: 杂志文章


    authors: Yi M,Nakamoto Y,Kaneko S,Yamashita T,Murakami S

    更新日期:1997-04-28 00:00:00

  • Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus.

    abstract::The genome of Lelystad virus (LV), a positive-strand RNA virus, is 15 kb in length and contains 8 open reading frames (ORFs) that encode putative viral proteins. ORFs 2 to 7 were cloned in plasmids downstream of the Sp6 RNA polymerase promoter, and the translation of transcripts generated in vitro yielded proteins tha...


    pub_type: 杂志文章


    authors: Meulenberg JJ,Petersen-den Besten A,De Kluyver EP,Moormann RJ,Schaaper WM,Wensvoort G

    更新日期:1995-01-10 00:00:00

  • Expression of authentic vaccinia virus-specific and inserted viral and cellular genes under control of an early vaccinia virus promoter is regulated post-transcriptionally in interferon-treated chick embryo fibroblasts.

    abstract::The interferon sensitivity of the expression of an influenza-virus hemagglutinin (HA) gene cloned into the thymidine kinase (TK) gene of vaccinia virus was studied in chick embryo fibroblasts (CEF) and Madin-Darby bovine kidney (MDBK) cells. In CEF, the expression of the HA gene is inhibited by pretreatment of cells w...


    pub_type: 杂志文章


    authors: Degen HJ,Blum D,Grün J,Jungwirth C

    更新日期:1992-05-01 00:00:00