Abstract:
:We investigated the origin and the effect of insertion D67D-THGERDLGPA within HIV-1 RT from a patient failing antiviral therapy. The insertion developed within the context of pre-existing NRTI and NNRTI mutations (M41L, L210W, T215Y and N348I). Concurrently, the NRTI mutations T69I and V118I and the NNRTI mutations K103N and Y181C were detected for the first time. High-level drug resistance (fold-changes≥50) and a good replication capacity (87% of wild-type) were observed, significantly higher than for the previous virus without insertion. The insertion was very similar to a region within human chromosome 17 (31/34 nucleotide identity), and had already been detected independently in a Japanese HIV-1 isolate. These results suggest that a particular sequence within human chromosome 17 is prone to horizontal gene transfer into the HIV-1 RT finger subdomain. This insertion confers selective advantage to HIV-1 by its contribution to multi-drug resistance and restoration of impaired replication capacity.
journal_name
Virologyjournal_title
Virologyauthors
Megens S,Vaira D,De Baets G,Dekeersmaeker N,Schrooten Y,Li G,Schymkowitz J,Rousseau F,Vandamme AM,Moutschen M,Van Laethem Kdoi
10.1016/j.virol.2014.03.023subject
Has Abstractpub_date
2014-05-01 00:00:00pages
310-8eissn
0042-6822issn
1096-0341pii
S0042-6822(14)00105-6journal_volume
456-457pub_type
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