Abstract:
:In this report, we examined the abilities and requirements of heterologous Gag proteins to functionally complement each other to support viral replication. Two distantly related gammaretroviruses, murine leukemia virus (MLV) and spleen necrosis virus (SNV), were used as a model system because SNV proteins can support MLV vector replication. Using chimeric or mutant Gag proteins that could not efficiently support MLV vector replication, we determined that a homologous capsid (CA) domain was necessary for the functional complementation of MLV and SNV Gag proteins. Findings from the bimolecular fluorescence complementation assay revealed that MLV and SNV Gag proteins were capable of colocalizing and interacting in cells. Taken together, our results indicated that MLV and SNV Gag proteins can interact in cells; however, a homologous CA domain is needed for functional complementation of MLV and SNV Gag proteins to complete virus replication. This requirement of homologous Gag most likely occurs at a postassembly step(s) of the viral replication.
journal_name
Virologyjournal_title
Virologyauthors
Lee SK,Boyko V,Hu WSdoi
10.1016/j.virol.2006.10.038subject
Has Abstractpub_date
2007-04-10 00:00:00pages
388-97issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00794-Xjournal_volume
360pub_type
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