Abstract:
:Myeloid differential primary response protein (MyD88) is a critical component in the signaling cascade through Toll-like receptors (TLRs) and is induced by alpha interferon (IFN-alpha). To examine the role of MyD88 in the antiviral activity of IFN-alpha against hepatitis B virus (HBV), we established MyD88 stably expressing cell lines and studied HBV replication in these lines after transient transfection. The levels of HBV proteins and viral replicative intermediates were effectively reduced in MyD88-expressing cells. A significant reduction of total and cytoplasmic viral RNAs in MyD88 stably expressing cells was also observed. Using a nuclear factor-kappaB (NF-kappaB) dependent reporter assay, it was shown that activation of NF-kappaB was moderately increased in the presence of expression of MyD88, and further significantly increased by co-expression of HBV. These results suggest a novel mechanism for the inhibition of HBV replication by IFN-alpha via expression of MyD88 protein involving activation of NF-kappaB signaling pathway and downregulation of viral transcription.
journal_name
Virologyjournal_title
Virologyauthors
Xiong W,Wang X,Liu X,Xiang L,Zheng L,Yuan Zdoi
10.1016/j.virol.2003.11.005subject
Has Abstractpub_date
2004-02-20 00:00:00pages
306-14issue
2eissn
0042-6822issn
1096-0341pii
S0042682203008353journal_volume
319pub_type
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