Abstract:
:Viruses of the family Luteoviridae are ssRNA plant viruses that have particles that exhibit icosahedral symmetry. To identify the residues that might be exposed on the surface of the Potato leafroll virus (PLRV; genus Polerovirus, family Luteoviridae) capsid, and therefore involved in biological interactions, we performed a structural analysis of the PLRV coat protein (CP) on the basis of comparisons with protein sequences and known crystal structures of CPs of other viruses. The CP of PLRV displays 33% sequence similarity with that of Rice yellow mottle virus (genus Sobemovirus) when the sequences were aligned by using the hidden Markov model method. A structure model for PLRV CP was designed by protein homology modeling, using the crystal structure of RYMV as a template. The resulting model is consistent with immunological and site-directed mutagenesis data previously reported. On the basis of this model it is possible to predict some surface properties of the PLRV CP and also speculate about the structural evolution of small icosahedral viruses.
journal_name
Virologyjournal_title
Virologyauthors
Terradot L,Souchet M,Tran V,Giblot Ducray-Bourdin Ddoi
10.1006/viro.2001.0900subject
Has Abstractpub_date
2001-07-20 00:00:00pages
72-82issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(01)90900-6journal_volume
286pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::We have previously reported that a virus, MH2-PA200, lacking the ability to transform quail embryo cells, could be isolated from wild type (wt) MH2 stocks passaged on chicken neuroretina cells. We report here the molecular cloning and extensive characterization of this MH2-PA200 provirus. Molecularly cloned MH2-PA200 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90030-9
更新日期:1986-09-01 00:00:00
abstract::The reticuloendotheliosis virus (REV) group of retroviruses and type D simian retroviruses (SRV) belong to the same receptor interference group. The cellular receptor for these viruses has not yet been identified. In order to study the distribution of the receptor and to identify a receptor negative cell line, vector ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1651
更新日期:1994-11-15 00:00:00
abstract::The role of bean common mosaic necrosis potyvirus (BCMNV) and lettuce mosaic potyvirus (LMV) proteins was investigated in terms of their capacity to function as viral movement proteins (MPs). Using Escherichia coli-expressed proteins and microinjection techniques, direct evidence was obtained that both the potyviral c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8777
更新日期:1997-10-27 00:00:00
abstract::Aster yellows virus (AYV) was recovered from hemolymph, alimentary canal, salivary glands and ovaries, but not from Malpighian tubules, mycetomes, fat body, testes, or brain of viruliferous leafhoppers, Macrosteles fascifrons (Stål). The presence of virus in the tissues was determined by injecting their extracts into ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90070-0
更新日期:1967-12-01 00:00:00
abstract::Viruses of the Norovirus genus (Caliciviridae family) are a major cause of human gastroenteritis. In some viruses, recombination is an important evolutionary process and therefore we should try to discover the quantity and characteristics of such events in Noroviruses. In order to identify recombination events, multip...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.09.051
更新日期:2006-02-05 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection can be inhibited by small molecules that target the CCR5 coreceptor. Here, we describe some properties of clonal viruses resistant to one such inhibitor, SCH-D, using both chimeric, infectious molecular clones and Env-pseudotypes. Studies using combinations of CCR5...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.11.004
更新日期:2007-04-25 00:00:00
abstract::Pseudorecombinants produced by exchanging genome components (DNAs A and B) of the geminiviruses African cassava mosaic virus (ACMV) and Indian cassava mosaic virus (ICMV), ACMV, and tomato golden mosaic virus (TGMV), and TGMV and abutilon mosaic virus (AbMV) are not infectious in their common host Nicotiana benthamian...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1523
更新日期:1993-10-01 00:00:00
abstract::The nucleocapsid (N) protein genes of vesicular stomatitis virus, serotypes New Jersey and Indiana, were expressed in Escherichia coli BL21(DE3) transformed by the recombinant expression vector pET-3a containing N genes under the control of the T7 RNA polymerase promoter. Following induction with isopropylthiogalactop...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1130
更新日期:1993-03-01 00:00:00
abstract::p53 is implicated in several cellular pathways such as induction of cell-cycle arrest, differentiation, senescence, and apoptosis. p53 is activated by a broad range of stress signals, including viral infections. While some viruses activate p53, others induce its inactivation, and occasionally p53 is differentially mod...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.08.008
更新日期:2020-11-01 00:00:00
abstract::KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.10.013
更新日期:2014-01-05 00:00:00
abstract::The host innate response to viral infection includes the production of interferons, which is dependent on the coordinated activity of multiple transcription factors. Herpes simplex virus 1 (HSV-1) has been shown to block efficient interferon expression by multiple mechanisms. We and others have demonstrated that HSV-1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.028
更新日期:2007-04-10 00:00:00
abstract::The regions of the AcMNPV genome represented as cytoplasmic transcripts early after infection of Spodoptera frugiperda cells prior to and encompassing the initiation of DNA replication were mapped. In vivo(32)P pulse-labeled cytoplasmic RNA from infected cells at various times postinfection was used to probe Southern ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90491-9
更新日期:1983-04-15 00:00:00
abstract::Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deep...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.023
更新日期:2015-11-01 00:00:00
abstract::The 50-70 S RNA complex of a clone of Kirsten murine leukemia virus (Ki-MLV), propagated in NIH 3T3 cells, as well as that of a Moloney (Mo)-MLV, propagated in a mouse thymus-bone marrow cell line, contained an approximately 30 S RNA of unknown biological activity in addition to 38 S genomic RNA. The approximately 30 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90225-2
更新日期:1977-11-01 00:00:00
abstract::We investigated the effect of selection pressures on evolution of HIV-1 pol in 51 patients after switching to a new antiretroviral combination reverse transcriptase (RT) inhibitor therapy. Evolution of the protease (PR) and RT reading frames were analysed separately. Pairwise evolutionary distances (ED) were calculate...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9774
更新日期:1999-06-20 00:00:00
abstract::The 5' leader region of the HIV-1 RNA contains the major 5' splice site (ss) that is used in the production of all spliced viral RNAs. This splice-donor (SD) region can fold a stem-loop structure. We demonstrate that whereas stabilization of this SD hairpin reduces splicing efficiency, destabilization increases splici...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.09.018
更新日期:2014-11-01 00:00:00
abstract::An improved purification procedure for human astrovirus serotype 2 (H-Ast2) has facilitated the isolation of a neutralizing monoclonal antibody (PL-2) directed against one of the major structural proteins (VP26) of H-Ast2. A minor component (VP29) of the virus particles is also recognized by PL-2 antibody. Immunofluor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1296
更新日期:1994-06-01 00:00:00
abstract::We cloned and sequenced the cDNAs against genomic RNAs and mRNAs for phosphoproteins (Ps) of human parainfluenza virus types 4A (PIV-4A) and 4B (PIV-4B). The PIV-4A and -4B P genes were 1535 nucleotides including poly(A) tract and were found to have two small open reading frames, neither of which was apparently large ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90413-l
更新日期:1990-09-01 00:00:00
abstract::Antiviral cytotoxic T-cells are critical for control of lymphocytic choriomeningitis virus (LCMV) infection in mice. In H-2b mice, the antiviral response is directed against three Db-restricted epitopes in the viral nucleoprotein (NP396-404) and glycoprotein (GP276-286 and GP33-41). Our present data revealed a clear h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8934
更新日期:1998-01-05 00:00:00
abstract::Infectious pancreatic necrosis is an important viral disease of salmonid fish reared in hatchery. Its etiological agent, IPNV, showed a high degree of antigenic heterogeneity. Up to 10 serotypes and 2 serogroups were proposed. Yet, very little is known about genomic variations among viruses of different origin. In ord...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1441
更新日期:1993-08-01 00:00:00
abstract::Two mutant strains of cucumber mosaic virus (CMV) were investigated with respect to virion stability and molecular determinants of aphid vector transmission. The mutant 2A1-MT-60x, derived from the mechanically passaged wild type 2A1-AT, is poorly transmissible by the aphid Aphis gossypii and not transmissible by the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0569
更新日期:2000-10-25 00:00:00
abstract::Immunodeficiency caused by HIV infection probably results from profound dysregulation of normal T lymphocyte properties by the virus. Despite description of the virus cytopathicity and numerous modifications in T cell functions, such as perturbation of antigen receptor signaling, CD4 downregulation, and induction of a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1124
更新日期:1994-03-01 00:00:00
abstract::There are more than 100 known adenovirus serotypes, including 50 human serotypes. They can infect all 5 major vertebrate classes but only Aviadenovirus infecting birds and Mastadenovirus infecting mammals have been well studied. CELO (chicken embryo lethal orphan) adenovirus is responsible for mild respiratory patholo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.05.011
更新日期:2008-08-15 00:00:00
abstract::Two forms of JC virus (JCV) have been isolated from its human host, an archetype found in kidney tissue and urine of nonimmunocompromised individuals and a rearranged type detected in lymphocytes and brain tissue of patients with and without progressive multifocal leukoencephalopathy. To investigate the hypothesis tha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0037
更新日期:1996-02-01 00:00:00
abstract::All tissue culture systems for propagating HBV employed so far make use of tandemly arranged HBV genomes usually under the control of strong foreign promoters. Thus these systems are helpful for virus production but are of limited value in the investigation of the regulation of HBV replication or of the extent to whic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0049
更新日期:1996-02-01 00:00:00
abstract::We recently showed that E6 protein of human papillomavirus (HPV) 16, a mucosal high-risk α-PV type, can potentiate Wnt/β-catenin/TCF signaling. Here we investigated the transcriptional activities of E6 proteins of cutaneous HPV types from the β and α genera. Results from reporter-gene assays showed that similar to HPV...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.05.020
更新日期:2017-09-01 00:00:00
abstract::We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90308-e
更新日期:1990-11-01 00:00:00
abstract::The epithelium of the cervix uteri has been reported to be frequently coinfected with both human papillomaviruses (HPV) and helper virus-dependent adenoassociated viruses (AAV). Seroepidemiological data suggest that AAV infection could inhibit cervical cancer that is caused by specific ("high-risk") types of papilloma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1256
更新日期:2002-02-01 00:00:00
abstract::The coat protein (CP) genes of all the strains of potato virus X (PVX) code for a protein of 25 kDa. Analysis of the CP by SDS-PAGE shows a migration mobility of 27 to 29 kDa, depending of the strain. Amino acid identity between some strains is too high to explain such abnormal migration by differences in primary stru...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1386
更新日期:1994-08-01 00:00:00
abstract::The immortalizing function of the human adenovirus 5 E1A oncoprotein requires efficient localization to the nucleus. In 1987, a consensus monopartite nuclear localization sequence (NLS) was identified at the C-terminus of E1A. Since that time, various experiments have suggested that other regions of E1A influence nucl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.08.014
更新日期:2014-11-01 00:00:00