Abstract:
:Herpes simplex virus type 1 (HSV-1) glycoproteins K and B (gK and gB) are intimately involved in virus-induced fusion of cells. Certain mutations in the UL27 (gB) and UL53 (gK) genes confer a syncytial (syn) phenotype characterized by extensive fusion of infected cells and giving rise to multinucleated cells. We have used HSV-1 syn mutants in conjunction with transfected cells or adenovirus vectors to overexpress wild-type gK or gB, in order to study the role of these proteins in virus-induced membrane fusion. Transient expression of wild-type gK inhibited fusion induced by HSV-1 encoding a mutant form of gK (syn gK) but not by viruses encoding a mutant form of gB (syn gB). Stably transformed cells expressing relatively high levels of gK suppressed cell fusion induced by HSV-1 mutants with lesions in the gK gene but not an HSV-1 with a syn mutation in the gB gene. In addition, there were marked reductions in the plaquing efficiencies and yields of HSV-1 on these cell lines. Cell fusion caused by HSV-1 syn20, a mutant encoding syn gK, was suppressed when cells were coinfected with an Ad vector, AdgK, which expresses wild-type gK. AdgK did not suppress fusion induced by HSV-1 KTTS.1, which expresses syn gB. Conversely, cells coinfected with AdgB, an Ad vector expressing a wild-type form of gB and HSV-1 KTTS.1 (syn gB) were not fused, whereas cells coinfected with AdgB and HSV-1 syn20 (syn gK) were fused. Expression of a number of other HSV-1 glycoproteins using Ad vectors did not reduce membrane fusion induced by syn gK or syn gB. Together, these results support models in which gK and gB participate directly in the fusion of HSV-infected cells. Mutant forms of gB and gK apparently disregulate the fusion process, whereas wild-type forms of gB and gK can act to suppress membrane fusion induced by their mutant counterparts.
journal_name
Virologyjournal_title
Virologyauthors
Hutchinson L,Graham FL,Cai W,Debroy C,Person S,Johnson DCdoi
10.1006/viro.1993.1507subject
Has Abstractpub_date
1993-10-01 00:00:00pages
514-31issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(83)71507-2journal_volume
196pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::A combination of linker scanning mutagenesis and deletional analyses has been used to determine the role of individual DNA-protein binding sites on expression from the hepatitis B virus (HBV) enhancer I-X promoter (map position (mp) 1042-1354, HBV adw2). Linker scanning mutation of the EF-C site caused a 67.5% drop in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1173
更新日期:1993-04-01 00:00:00
abstract::Without a virus culture system, genetic analysis becomes the principal method to classify norovirus (NoV) strains. Currently, classification of NoV strains beneath the species level has been based on sequences from different regions of the viral genome. As a result, the phylogenetic insights of some virus were not app...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.015
更新日期:2006-03-15 00:00:00
abstract::BS-1, a stroma cell line derived from normal human bone marrow, can support the growth of murine erythroid (BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-Meg) progenitor cells in a short-term in vitro coculture system. Exposure of BS-1 cell to the human immunodeficiency virus (HIV) prior to coculture...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1159
更新日期:1993-03-01 00:00:00
abstract::Following a bite from an infected tick, tick-borne flaviviruses cause encephalitis, meningitis and hemorrhagic fever in humans. Although these viruses spend most of their time in the tick, little is known regarding the virus-vector interactions. We developed a simple method for synchronously infecting Ixodes scapulari...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.057
更新日期:2007-09-01 00:00:00
abstract::We previously showed that reduced infectivity of HIV with incompletely processed capsid-spacer protein 1 (CA-SP1) is rescued by cellular activation or increased expression of HSP90AB1, a member of the cytosolic heat shock protein 90 family. Here we show that HSP90AB1 is present in HIV virions and that HSP90AB1, but no...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.005
更新日期:2013-02-05 00:00:00
abstract::There is no effective vaccine for Marburg virus (MBGV) or any other filovirus, nor enough pertinent information to expedite rational vaccine development. To ascertain some of the minimal requirements for a MBGV vaccine, we determined whether whole inactivated MBGV, or a baculovirus-expressed virion subunit, could be u...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8883
更新日期:1997-12-08 00:00:00
abstract::The nucleotide sequences of the genomes of two strains of canine adenovirus, type 1 (Glaxo and CLL) have been determined within the region spanning approximately 0.80-0.91 map unit. Homology comparisons with other adenoviral DNAs indicate that this region contains part of the hexon-associated protein (pVIII) precursor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90142-x
更新日期:1991-07-01 00:00:00
abstract::A 140,000-dalton disulfide-linked glycoprotein (gp140) specified by varicella-zoster virus (VZV) in infected cultured cells was identified and precipitated by two murine monoclonal antibodies (VZ-151 and VZ-158). When analyzed under reducing conditions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gp14...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90098-9
更新日期:1984-01-15 00:00:00
abstract::Transcription of human papillomaviruses (HPV) is regulated by enhancer sequences located in the upstream regulatory region. The factors regulating expression of one of the high risk genital HPV types, HPV 31b, were investigated using transient expression and protein binding assays. A region of 262 base pairs in length...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1390
更新日期:1995-08-01 00:00:00
abstract::Long-term non-progressive HIV infection, characterized by low but detectable viral load and stable CD4 counts in the absence of antiviral therapy, is observed in about 5% of HIV-infected patients. Here we identified four therapy naïve individuals who are strongly seropositive for HIV-1 but who lack evidence of detecta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.06.001
更新日期:2005-08-15 00:00:00
abstract::The Chlorella virus PBCV-1 genome is a linear nonpermuted 333-kbp dsDNA molecule with covalently closed hairpin termini. The termini (minus the hairpin) are identical inverted repeats of at least 2185 bases after which the sequence diverges. The inverted repeats contain two small potential open reading frames and seve...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90089-t
更新日期:1991-02-01 00:00:00
abstract::Thirteen temperature-sensitive (ts) mutants of human cytomegalovirus (HCMV) have been isolated after mutagenesis with nitrosoguanidine or ultraviolet light. Four mutants defective in viral DNA synthesis at nonpermissive temperature (DNA-) were classified into two separate complementation groups. The other nine HCMV ts...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90257-1
更新日期:1987-05-01 00:00:00
abstract::The 5'-terminal noncoding region sequences were determined for the genome RNAs of seven strains of St. Louis encephalitis virus (SLEV) and one strain of West Nile virus (WNV) using a single synthetic cDNA primer complementary to the 5'-terminus of the coding region of a strain of WNV RNA. The 5'-terminal sequences obt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90468-0
更新日期:1988-02-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) latency associated transcript (LAT) gene's anti-apoptosis activity plays a central, but not fully elucidated, role in enhancing the virus's reactivation phenotype. In transient transfection experiments, LAT increases cell survival following an apoptotic insult in the absence of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.07.023
更新日期:2007-12-05 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is a member of the Gammaherpesvirinae and is causally associated with Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. The KSHV genome encodes over 85 genes; the function of some is entirely unknown. We have characterized the transcriptio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.023
更新日期:2008-02-05 00:00:00
abstract::Characterization of the regions of human papillomaviruses (HPVs) that elicit neutralizing immune responses supports studies on viral infectivity and provides insight for the development and evaluation of prophylactic vaccines. HPV11 is a major etiologic agent of genital warts and a likely vaccine candidate. A conforma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0083
更新日期:2000-01-20 00:00:00
abstract::The requirement to replicate in both vertebrate and invertebrate hosts is thought to limit the introduction of genetic changes into the genome of arboviruses. Serial passage under laboratory conditions will overcome this limitation allowing for genetic changes to be introduced and affecting the virulence of the virus ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.030
更新日期:2011-06-20 00:00:00
abstract::The mitotic spindle assembly checkpoint (SAC) ensures faithful chromosome segregation during mitosis by censoring kinetochore-microtubule interactions. It is frequently rendered dysfunctional during carcinogenesis causing chromosome missegregation and genomic instability. There are conflicting reports whether the HPV1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.06.006
更新日期:2012-10-10 00:00:00
abstract::The adenine DNA methyltransferase M.CviRI (TGCmA) gene from chlorella virus XZ-6E was cloned into the Autographa californica nuclear polyhedrosis virus (AcMNPV) genome and expressed in Spodoptera frugiperda insect cells under the control of two tandemly arranged viral promoters, the early ETL promoter and the late pol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1539
更新日期:1993-10-01 00:00:00
abstract::Epstein-Barr virus (EBV) is widely spread in the human population. EBV nuclear antigen 1 (EBNA1) is a transcription factor that activates viral genes and is necessary for viral replication and partitioning, which binds the EBV genome cooperatively. We identify similar EBNA1 repeat binding sites in the human genome usi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.040
更新日期:2010-09-30 00:00:00
abstract::Poliovirus readily establishes a persistent infection in the K562-Mu erythroleukemia cell strain. In this study, three additional K562 cell strains were analyzed for their responses to poliovirus infection and found to be quite variable. K562 cells obtained from the ATCC established a persistent infection, similar to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1541
更新日期:1995-10-20 00:00:00
abstract::The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90695-l
更新日期:1992-07-01 00:00:00
abstract::Vaccinia virus (VV) encodes a 37-kDa envelope protein (p37) that is palmitylated on cysteine residues 185186 of the 372-amino acid protein. We have previously reported on a loosely conserved consensus motif. Further analysis has identified a conserved consensus sequence, Hydro*AAC(C)A (Hydro* represents a hydrophobic ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9543
更新日期:1999-02-01 00:00:00
abstract::The early steps of Rous sarcoma virus-Bryan strain (BS-RSV) infection of genetically resistant and susceptible chick embryo cells were investigated. The methods used were the uptake of virus by cells grown in monolayer, and the ability of infected cells to form foci of transformed cells after plating on resistant and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90046-3
更新日期:1967-08-01 00:00:00
abstract::The mechanism and machinery of hepatitis C virus (HCV) RNA replication are still poorly characterized. Our previous study has shown that HCV RNA synthesis occurs on a lipid raft membrane structure [J. Virol. 77 (2003) 77 4160]. In this study, we further characterized these replication complexes (RCs) in Huh-7 cells th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.03.034
更新日期:2004-07-01 00:00:00
abstract::The sequences of 189 residues from the 3' terminus of three RNAs of one strain and two RNAs of another strain of tomato aspermy virus have been determined; there was almost complete sequence homology between the RNAs. A base-paired transfer RNA-like structure is proposed for tomato aspermy virus RNAs which is similar ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90639-5
更新日期:1981-07-15 00:00:00
abstract::In this study we report the development and optimization of two minigenome rescue systems for Nipah virus, a member of the Paramyxoviridae family. One is mediated by the T7 RNA polymerase supplied either by a constitutively expressing cell line or by transfection of expression plasmids and is thus independent from inf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.008
更新日期:2008-01-05 00:00:00
abstract::Segment 7 (M) of the cold-adapted live influenza A virus vaccine plays a primary role in the ability of this virus to interfere with the replication of wild-type influenza A viruses. This conclusion is based on several lines of evidence. Single gene reassortant viruses derived by crossing influenza A/Ann Arbor/6/60 (H...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90011-y
更新日期:1991-01-01 00:00:00
abstract::Human cytomegalovirus (HCMV) glycoprotein B (gB) promotes virion entry into cells by fusing the virion envelope with the cellular membrane. We recently reported that UB cells (U373 glioblastoma cells constructed to produce HCMV gB constitutively) form multinucleate syncytia that are dependent on the density of gB in t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1290
更新日期:1995-06-01 00:00:00
abstract::Studies of the cellular autophagy pathway have exploded over the past twenty years. Now appreciated as a constitutive degradative mechanism that promotes cellular homeostasis, autophagy is also required for a variety of developmental processes, cellular stress responses, and immune pathways. Autophagy certainly acts a...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2015.03.042
更新日期:2015-05-01 00:00:00