Abstract:
:Understanding the mechanism of HIV-1 latency is crucial to the viral reservoir eradication. Human cellular miRNAs can modulate HIV-1 expression by targeting of viral RNAs or host gene transcripts. To identify miRNAs modulating HIV-1 latency, we determined the miRNA expression profiles of HIV-1 latently infected and productively infected cells by microarray and qRT-PCR. Among the differentially expressed miRNAs, miR-196b and miR-1290 targeted the 3' untranslated region of HIV-1 and affected its expression. Ectopic expression of these two miRNAs efficiently suppressed HIV-1 production and infectivity. Specific inhibitors of these miRNAs substantially counteracted their effects on HIV-1, as measured either as viral production and infectivity in HEK-293T cells or as HIV-1 RNA expression or viral production in cells isolated from HIV-1-infected individuals. Our study emphasizes the role of cellular miRNAs in HIV-1 latency regulation, and it suggests that inhibitors of miR-196b and miR-1290 could be used to activate latent HIV-1.
journal_name
Virologyjournal_title
Virologyauthors
Wang P,Qu X,Zhou X,Shen Y,Ji H,Fu Z,Deng J,Lu P,Yu W,Lu H,Zhu Hdoi
10.1016/j.virol.2015.09.016subject
Has Abstractpub_date
2015-12-01 00:00:00pages
228-38eissn
0042-6822issn
1096-0341pii
S0042-6822(15)00407-9journal_volume
486pub_type
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