Abstract:
:A cDNA encoding the entire amino acid sequence of the G glycoprotein of respiratory syncytial virus (RSV) was inserted into a yeast-Escherichia coli shuttle vector such that expression of the virus gene was regulated by the yeast GAL1 promoter. Transformation of Saccharomyces cerevisiae with the vector led to the formation of the G protein when cells were grown in the presence of galactose. Under these conditions the RSV G appeared as a 60- to 65-kDa glycosylated protein. Expression of the G cDNA in secretory mutants of S. cerevisiae yielded a protein of 35 kDa in a mutant unable to glycosylate secreted proteins and a 65-kDa polypeptide in a mutant unable to transport proteins beyond the endoplasmic reticulum. The RSV protein formed in the latter mutant was converted to a 60-kDa protein by endoglycosidase H. Our results show that yeast can recognize the internal signal sequence of RSV G protein and add glycosyl groups to the polypeptide in the endoplasmic reticulum. Evidence is presented for both N- and O-linked glycosylation of the virus glycoprotein.
journal_name
Virologyjournal_title
Virologyauthors
Ding MX,Wen DZ,Schlesinger MJ,Wertz GW,Ball LAdoi
10.1016/0042-6822(87)90485-5subject
Has Abstractpub_date
1987-08-01 00:00:00pages
450-3issue
2eissn
0042-6822issn
1096-0341journal_volume
159pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The genome of the Chilo iridescent virus (CIV) was analyzed for existence of repetitive DNA sequences by DNA-DNA hybridization using a defined and complete gene library of the viral genome (209 kbp) and by heteroduplex mapping. These experiments revealed the presence of repetitive DNA elements in the CIV genome, which...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phag...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90130-2
更新日期:1984-05-01 00:00:00
abstract::We investigated the localization of host mRNA during Rift Valley fever virus (RVFV) infection. Fluorescence in situ hybridization revealed that infection with RVFV altered the localization of host mRNA. mRNA accumulated in the nuclei of RVFV-infected but not mock-infected cells. Further, overexpression of the NSS gene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.09.003
更新日期:2015-12-01 00:00:00
abstract::Next generation sequence analyses were used to assess virus-derived small RNA (vsRNA) profiles for Homalodisca coagulata virus-1 (HoCV-1), family Dicistroviridae, and Homalodisca vitripennis reovirus (HoVRV), family Reoviridae, from virus-infected H. vitripennis, the glassy-winged sharpshooter. The vsRNA reads were ma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.04.005
更新日期:2013-07-20 00:00:00
abstract::Putative replication complexes of cauliflower mosaic virus (CaMV) which resemble hepatitis B virus replication complexes in several respects have been identified. The CaMV replication complexes contain a heterogeneous population of minus-strand DNA ranging in size from less than 600 nucleotides to full genome length o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90109-6
更新日期:1985-05-01 00:00:00
abstract::A combination of linker scanning mutagenesis and deletional analyses has been used to determine the role of individual DNA-protein binding sites on expression from the hepatitis B virus (HBV) enhancer I-X promoter (map position (mp) 1042-1354, HBV adw2). Linker scanning mutation of the EF-C site caused a 67.5% drop in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1173
更新日期:1993-04-01 00:00:00
abstract::The human immunodeficiency virus (HIV-1) causes various hematopoietic abnormalities, with thrombocytopenia (TP) occurring in 30% of infected individuals. In the present study, we aimed to determine whether HIV-1 in the bone marrow of TP patients can infect primary megakaryocytes in vitro, which may contribute to the d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0193
更新日期:2000-03-30 00:00:00
abstract::We describe a novel virus, AFV1, of the hyperthermophilic archaeal genus Acidianus. Filamentous virions are covered with a lipid envelope and contain at least five different proteins with molecular masses in the range of 23-130 kDa and a 20.8-kb-long linear double-stranded DNA. The virus has been assigned to the famil...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00481-1
更新日期:2003-10-10 00:00:00
abstract::Morphological and molecular changes produced by Autographa californica nuclear polyhedrosis virus (AcMNPV) infection in a permissive cell line, IPLB-SF-21AE (SF-21), of Spodoptera frugiperda and a nonpermissive cell line, FPMI-CF-203 (CF-203), of Choristoneura fumiferana are described. CF-203 cells inoculated with AcM...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0411
更新日期:1996-08-01 00:00:00
abstract::The amino acid sequence of BMV (brome mosaic virus) coat protein is presented and compared with the sequence derived from RNA structure. Apart from removal of the N-terminal methionine residue and acetylation of the following serine residue, no post-translational modifications occur. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90193-9
更新日期:1983-09-01 00:00:00
abstract::The virus SIRV1 of the extremely thermophilic archaeon Sulfolobus has a double-stranded DNA genome similar in architecture to the genomes of eukaryal viruses of the families Poxviridae, Pycodnaviridae, and Asfarviridae: the two strands of the 32,301 bp long linear genome are covalently connected forming a continuous p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0776
更新日期:2001-03-01 00:00:00
abstract::Packaging of lambda DNA is mediated by the phage-encoded enzyme, terminase, which acts at a site termed cos. cos consists of cosB, the site where terminase binds lambda DNA, and cosN, the site where nicks are introduced to generate the cohesive ends of virion DNA. cos contains multiple binding sites for gpNu1, the sma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90085-6
更新日期:1990-01-01 00:00:00
abstract::In a previous study on the replication of Kunjin virus using immunoelectron microscopy (E. G. Westaway, J. M. Mackenzie, M. T. Kenney, M. K. Jones, and A. A. Khromykh, 1997, J. Virol. 71, 6650-6661), NS1 and NS3 were found associated with double-stranded RNA (dsRNA) within vesicle packets (VP) in infected Vero cells, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9156
更新日期:1998-06-05 00:00:00
abstract::In retrovirus-induced tumors, proviral DNA is commonly found next to or within a cellular proto-oncogene such that transcription of the gene is influenced by the viral promoter or enhancer. Extensive surveys of naturally occurring tumors reveal that proviruses integrated on the 3' side of the gene are usually in the s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1245
更新日期:1993-05-01 00:00:00
abstract::The PBj14 isolate of simian immunodeficiency virus, SIVsmmPBj14, induces an acutely lethal disease in experimentally inoculated pigtailed macaques, that is characterized by severe enteropathy and extensive immune activation, particularly within gut-associated lymphoid tissue (GALT). Experiments were conducted to deter...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0571
更新日期:1996-11-01 00:00:00
abstract::The 3' ends of three genomic RNAs (gRNAs) of cowpea chlorotic mottle virus (CCMV) terminate in a highly conserved tRNA-like structure (3'TLS). To examine the intrinsic role played the 3'TLS in packaging, the competence of each gRNA lacking the 3' TLS (DeltaTLS-gRNA) to interact with dissociated coat protein (CP) subun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.12.009
更新日期:2005-02-20 00:00:00
abstract::The 682-nt satellite DNA (sat-DNA) of Tomato leaf curl virus (TLCV) depends on the helper virus for its replication. In contrast to the strict specificity that exists in each geminivirus for its cognate replication associated protein (Rep), TLCV sat-DNA can utilize Rep encoded by distinct geminiviruses. We have used a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1671
更新日期:2003-01-20 00:00:00
abstract::A chloroplast-localized papaya methionine sulfoxide reductase B1 (PaMsrB1) interacting with Papaya ringspot virus (PRSV) NIa-Pro was identified using a Sos recruitment two-hybrid system (SRS). SRS analysis of several deletion mutants of PRSV NIa-Pro and PaMsrB1 demonstrated that the C-terminal (residues 133-239) fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.007
更新日期:2012-12-05 00:00:00
abstract::Monoclonal antibodies specific for eight antigenic sites on the G1 glycoprotein of La Crosse virus were studied in a kinetics of neutralization assay. When examined at the same protein concentration, there were differences in the rates and degree of neutralization induced by these antibodies. The maximum amount of neu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90163-6
更新日期:1984-07-30 00:00:00
abstract::We describe studies of woodchuck hepatitis virus nucleic acids in liver and other tissues of chronically infected woodchucks, using Southern and Northern blot hybridization techniques. Single-stranded and covalently closed circular replicative DNA molecules were distinguished from partly double-stranded virus genomes....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90035-4
更新日期:1989-03-01 00:00:00
abstract::Genetic variants surrounding the interferon-λ3 (IFNL3) gene are strongly associated with clearance of hepatitis C virus (HCV). A variant (rs368234815 TT/ΔG) upstream of IFNL3 was recently implicated to control expression of a novel gene termed IFNL4. We conducted genetic analysis of rs368234815 in a chronic HCV patien...
journal_title:Virology
pub_type: 临床试验,杂志文章
doi:10.1016/j.virol.2014.12.020
更新日期:2015-02-01 00:00:00
abstract::We have previously cloned and analyzed the structure of a type 16 human papillomavirus (HPV16) integration in a primary cervical carcinoma tissue, M50 (Choo et al., J. Virol. 62, 1659-1666, 1988). We found that specific nucleotide sequences within the HPV16 genome influenced the genomic organization of the integrated ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90366-y
更新日期:1990-10-01 00:00:00
abstract::Tombusviruses replicate on pre-existing organelles such as peroxisomes or mitochondria, the membranes of which become extensively reorganized into multivesicular bodies (MVBs) during the infection process. Cucumber necrosis virus (CNV) has previously been shown to replicate in association with peroxisomes in yeast. We...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.12.035
更新日期:2014-03-01 00:00:00
abstract::The biological phenotype of HIV-1 isolates from 21 subjects with primary HIV-1 infection was determined in primary lymphocytes and monocyte-derived macrophages as well as in three T-cell lines (MT-2, HUT78, and C8166). Seven isolates (33%) replicated on at least one cell line and were classified as rapid/high (n = 6) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1534
更新日期:1994-10-01 00:00:00
abstract::Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.12.005
更新日期:2019-02-01 00:00:00
abstract::Herpes simplex virus encodes proteins, such as DNA polymerase, that are essential for its replication and proteins, such as thymidine kinase, that are not essential for replication in cell culture, but are important for pathogenesis in animal models. However, certain mutations affecting these proteins exert little or ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80041-7
更新日期:1995-01-10 00:00:00
abstract::The human parvovirus B19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. So far, it has not been possible to grow B19 virus in cultured cells. Here we report the use of in vitro transcription in HeLa cell extracts and transient expression of cloned DNA transfected into HeLa ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90297-2
更新日期:1987-04-01 00:00:00
abstract::We have previously demonstrated that the bZIP transcription factor CREB-2, also called ATF-4, trans-activates, in association with the viral protein Tax, the human T-cell leukemia virus type I (HTLV-I) promoter. In this study, we have examined whether CREB-2 acetylation affects transcriptional activation mediated by T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1501
更新日期:2002-08-01 00:00:00
abstract::Epstein-Barr virus (EBV) is widely spread in the human population. EBV nuclear antigen 1 (EBNA1) is a transcription factor that activates viral genes and is necessary for viral replication and partitioning, which binds the EBV genome cooperatively. We identify similar EBNA1 repeat binding sites in the human genome usi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.040
更新日期:2010-09-30 00:00:00
abstract::The coat protein (CP) of alfalfa mosaic virus (AMV) strain 425 assembles to bacilliform or rod-shaped particles in the presence of nucleic acids or to T = 1 empty icosahedral particles in the absence of nucleic acids. To study the determinants of CP assembly, recombinant CPs (rCPs) that contained a (His)(6) region wer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9805
更新日期:1999-07-20 00:00:00