Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission.


:A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently enhance this process. These data indicate that there are features common to B cells and DCs that facilitate transmission of HIV-1 and provide new insights toward the mechanism of DC-SIGN-mediated HIV-1 transmission.






Wu L,Martin TD,Carrington M,KewalRamani VN




Has Abstract


2004-01-05 00:00:00
















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    pub_type: 杂志文章


    authors: Yu B,Dong J,Wang C,Zhan Y,Zhang H,Wu J,Kong W,Yu X

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  • Sequence analysis of the phosphoprotein (P) genes of human parainfluenza type 4A and 4B viruses and RNA editing at transcript of the P genes: the number of G residues added is imprecise.

    abstract::We cloned and sequenced the cDNAs against genomic RNAs and mRNAs for phosphoproteins (Ps) of human parainfluenza virus types 4A (PIV-4A) and 4B (PIV-4B). The PIV-4A and -4B P genes were 1535 nucleotides including poly(A) tract and were found to have two small open reading frames, neither of which was apparently large ...


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    authors: Kondo K,Bando H,Tsurudome M,Kawano M,Nishio M,Ito Y

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  • Subviral agents associated with plant single-stranded DNA viruses.

    abstract::Begomoviruses (family Geminiviridae) are responsible for many economically important crop diseases worldwide. The majority of these diseases are caused by bipartite begomovirus infections, although a rapidly growing number of diseases of the Old World are associated with monopartite begomoviruses. With the exception o...


    pub_type: 杂志文章,随机对照试验,评审


    authors: Briddon RW,Stanley J

    更新日期:2006-01-05 00:00:00

  • Single oral immunization with replication deficient recombinant adenovirus elicits long-lived transgene-specific cellular and humoral immune responses.

    abstract::Oral-gastric delivery of vaccines is a preferred route of immunization and is particularly relevant to the development of vaccine-vector systems. We have investigated the ability of a replication deficient (E1-deleted) adenovirus construct (RAd68), which efficiently expresses the measles virus nucleocapsid (N) protein...


    pub_type: 杂志文章


    authors: Sharpe S,Fooks A,Lee J,Hayes K,Clegg C,Cranage M

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  • Infection of bovine dendritic cells by rinderpest or measles viruses induces different changes in host transcription.

    abstract::The morbilliviruses are a closely related genus which are very similar in their sequences and share a common receptor, but nevertheless show significant restriction in the host species in which they cause disease. One contribution to this restriction might be the nature of the hosts' responses to infection. We have us...


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    authors: Nanda SK,Baron J,Royall E,Robinson L,Falciani F,Baron MD

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  • Characterization of Enterococcus faecium bacteriophage IME-EFm5 and its endolysin LysEFm5.

    abstract::Due to the worldwide prevalence of antibiotic resistant strains, phages therapy has been revitalized recently. In this study, an Enterococcus faecium phage named IME-EFm5 was isolated from hospital sewage. Whole genomic sequence analysis demonstrated that IME-EFm5 belong to the Siphoviridae family, and has a double-st...


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    authors: Gong P,Cheng M,Li X,Jiang H,Yu C,Kahaer N,Li J,Zhang L,Xia F,Hu L,Sun C,Feng X,Lei L,Han W,Gu J

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  • The crystal structure of HIV CRF07 B'/C gp41 reveals a hyper-mutant site in the middle of HR2 heptad repeat.

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    pub_type: 杂志文章


    authors: Du J,Xue H,Ma J,Liu F,Zhou J,Shao Y,Qiao W,Liu X

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  • Ex vivo detection of adenovirus specific CD4+ T-cell responses to HLA-DR-epitopes of the Hexon protein show a contracted specificity of T(HELPER) cells following stem cell transplantation.

    abstract::Human adenovirus (HAdV) is a cause of significant morbidity and mortality in immunocompromised patients, especially after stem cell transplantation (SCT). Viral clearance has been attributed to CD4(+) T-cell responses against the Hexon-protein, but the frequency of specific T(HELPER) cells is extremely low or not dete...


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    authors: Serangeli C,Bicanic O,Scheible MH,Wernet D,Lang P,Rammensee HG,Stevanovic S,Handgretinger R,Feuchtinger T

    更新日期:2010-02-20 00:00:00

  • Common modular structure of lentivirus LTRs.

    abstract::Retroviruses are expressed under the control of viral control regions designated long terminal repeats (LTRs), which contain all signals for transcriptional initiation as well as transcriptional termination. However, retroviral LTRs from different species within a common genus, such as Lentivirus, do not show signific...


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    authors: Frech K,Brack-Werner R,Werner T

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  • Mutational analysis of the coat protein gene of tobacco mosaic virus in relation to hypersensitive response in tobacco plants with the N' gene.

    abstract::Tomato strain L of tobacco mosaic virus (TMV-L) induces a hypersensitive response (necrotic local lesions) on tobacco plants with the N' gene. A factor responsible for induction of the hypersensitive response has been mapped to the coat protein gene. We have constructed several mutants which have insertions or deletio...


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    authors: Saito T,Yamanaka K,Watanabe Y,Takamatsu N,Meshi T,Okada Y

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  • Carboxy-fluorescein diacetate, succinimidyl ester labeled papillomavirus virus-like particles fluoresce after internalization and interact with heparan sulfate for binding and entry.

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    pub_type: 杂志文章


    authors: Drobni P,Mistry N,McMillan N,Evander M

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  • Antigenic, sequence, and crystal variation in influenza B neuraminidase.

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    pub_type: 杂志文章


    authors: Air GM,Laver WG,Luo M,Stray SJ,Legrone G,Webster RG

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  • Viral isolates derived from simian varicella epizootics are genetically related but are distinct from other primate herpesviruses.

    abstract::Epizootics of a natural varicella-like disease occur in populations of nonhuman primates. Several primate herpesviruses have been isolated from these epizootics, but the relatedness of these isolates to each other is not well-defined. In this study, we demonstrated that the restriction endonuclease (REn) profiles of f...


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    authors: Gray WL,Gusick NJ

    更新日期:1996-10-01 00:00:00

  • Deletion of glycoprotein gE reduces the propagation of pseudorabies virus in the nervous system of mice after intranasal inoculation.

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    pub_type: 杂志文章


    authors: Babic N,Klupp B,Brack A,Mettenleiter TC,Ugolini G,Flamand A

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    authors: Milks ML,Washburn JO,Willis LG,Volkman LE,Theilmann DA

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    authors: Feeney KM,Saade A,Okrasa K,Parish JL

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    authors: Hötzel I,Cheevers WP

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  • Characterization of the interactions among vaccinia virus transcription factors G2R, A18R, and H5R.

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    authors: Black EP,Moussatche N,Condit RC

    更新日期:1998-06-05 00:00:00

  • Natural controlled HIV infection: preserved HIV-specific immunity despite undetectable replication competent virus.

    abstract::Long-term non-progressive HIV infection, characterized by low but detectable viral load and stable CD4 counts in the absence of antiviral therapy, is observed in about 5% of HIV-infected patients. Here we identified four therapy naïve individuals who are strongly seropositive for HIV-1 but who lack evidence of detecta...


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    authors: Kloosterboer N,Groeneveld PH,Jansen CA,van der Vorst TJ,Koning F,Winkel CN,Duits AJ,Miedema F,van Baarle D,van Rij RP,Brinkman K,Schuitemaker H

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  • Histones as a target for influenza virus matrix protein M1.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Hardy ME,Gorziglia M,Woode GN

    更新日期:1992-11-01 00:00:00

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    pub_type: 杂志文章


    authors: Baba TW,Humphries EH

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    pub_type: 杂志文章


    authors: Perrault J,Holland JJ

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