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Cloning and expression of NDV hemagglutinin-neuraminidase cDNA in a baculovirus expression vector system.

Abstract：

:The hemagglutinin-neuraminidase (HN) gene of the Hitchner B1 strain of Newcastle disease virus (NDV) was cloned as a cDNA and inserted into a baculovirus expression vector. The recombinant HN (recHN) expressed in Spodoptera frugiperda cells had both hemagglutinating and neuraminidase activities both of which were inhibited by polyclonal anti-NDV sera or a monoclonal antibody (MAb) against HN. Infected insect cells could hemadsorb chicken red blood cells suggesting that the recHN is properly glycosylated and transported to the cell surface. A 67-kDa recHN precursor and a 74-kDa, presumably mature, recHN from infected cells were detected by Western blot analysis and were found to comigrate with similar proteins from NDV-infected chick embryo fibroblast cells. The kinetics of synthesis of recHN was similar to that for polyhedrin and some HN appeared in the extracellular medium. HN was copurified with extracellular virus (ECV) from the extracellular medium and was used to immunize chickens. The anti recHN serum was specific to NDV in both ELISA and Western blot analysis.

journal_name

Virology

journal_title

Virology

authors

Nagy E,Derbyshire JB,Dobos P,Krell PJ

doi

10.1016/0042-6822(90)90012-g

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

426-38

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

176

pub_type

杂志文章

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