The high levels of p53 present in adenovirus early region 1-transformed human cells do not cause up-regulation of MDM2 expression.


:The cellular protein MDM2 can bind to the tumor suppressor gene product p53 and abrogate its transcriptional activity. In addition, p53 can regulate expression of the mdm2 gene. We and others have previously shown that p53 is present at high levels in adenovirus-transformed cells which express the larger E1B protein. In view of these observations the expression of MDM2 in a panel of adenovirus transformed human cell lines has been examined. Two major species (98K and 80K) were detected, together with a number of minor species of higher and lower molecular weight. While there was little variation in levels of 98K protein between cell lines, appreciable differences in the expression of the 80K component were apparent. There was no correlation between MDM2 and p53 expression in any of the adenovirus transformants, nor with the viral proteins expressed. The pattern and level of MDM2 detected was similar to that seen in human tumor cell lines and in human fetal tissue. Northern blot analysis suggested that MDM2 expression was regulated at the transcriptional level. Stable interactions were observed between p53 and MDM2 in the adenovirus-transformed cell lines and in Ad5 E1 HEK 293 cells a ternary complex of p53, MDM2, and the Ad5 E1B 58K protein was demonstrated. In view of the lack of correlation between the level of p53 and MDM2 in adenovirus E1-transformed cells, the capacity of p53 to cause transcriptional activation was assessed using transfected CAT constructs linked to p53 responsive elements. p53 transcriptional activity was similar in all of the cell lines examined and did not correlate with protein expression. It is concluded, on the basis of all of these data, that the high concentrations of p53 found in adenovirus transformants are not transcriptionally active and have no influence on MDM2 expression. However, when expression of p53 was increased following infection with mutant adenoviruses, which do not express the larger E1B proteins, there was an appreciable increase in p53 transcriptional activity and in the levels of all of the MDM2 components.






Grand RJ,Lecane PS,Owen D,Grant ML,Roberts S,Levine AJ,Gallimore PH




Has Abstract


1995-07-10 00:00:00
















  • Role of a heterologous retroviral transport element in the development of genetic complementation assay for mouse mammary tumor virus (MMTV) replication.

    abstract::The mouse mammary tumor virus (MMTV) is a type B retrovirus that is unique from other retroviruses in having multiple "tissue specific" and "hormone inducible" promoters. This unique feature has lead to the increasing interest in studying the biology of MMTV replication with the ultimate goal of developing MMTV based ...


    pub_type: 杂志文章


    authors: Rizvi TA,Ali J,Phillip PS,Ghazawi A,Jayanth P,Mustafa F

    更新日期:2009-03-15 00:00:00

  • Productive vs non-productive infection by cell-free varicella zoster virus of human neurons derived from embryonic stem cells is dependent upon infectious viral dose.

    abstract::Varicella Zoster virus (VZV) productively infects humans causing varicella upon primary infection and herpes zoster upon reactivation from latency in neurons. In vitro studies using cell-associated VZV infection have demonstrated productive VZV-infection, while a few recent studies of human neurons derived from stem c...


    pub_type: 杂志文章


    authors: Sloutskin A,Kinchington PR,Goldstein RS

    更新日期:2013-09-01 00:00:00

  • Quasispecies, error catastrophe, and the antiviral activity of ribavirin.

    abstract::Ribavirin is the first synthetic, broad-spectrum antiviral nucleoside. Despite its more than 30 year history, the mechanism of action of this compound remains unclear and somewhat controversial. Recent data suggest the possibility that the activity of ribavirin against RNA viruses is a reflection of incorporation of r...


    pub_type: 杂志文章,评审


    authors: Graci JD,Cameron CE

    更新日期:2002-07-05 00:00:00

  • Viral metagenomics in Brazilian Pekin ducks identifies two gyrovirus, including a new species, and the potentially pathogenic duck circovirus.

    abstract::Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our under...


    pub_type: 杂志文章


    authors: Cibulski S,Weber MN,de Sales Lima FE,Lima DA,Fernandes Dos Santos H,Teixeira TF,Varela APM,Tochetto C,Mayer FQ,Roehe PM

    更新日期:2020-09-01 00:00:00

  • Polyomavirus JC infects human brain microvascular endothelial cells independent of serotonin receptor 2A.

    abstract::Although human polyomavirus JC (JCV) is known to cause progressive multifocal leukoencephalopathy (PML) in immunocompromised individuals, the mechanism by which JCV crosses the blood-brain barrier (BBB) remains unclear. To test our hypothesis that cell-free JCV gains entry into the brain by infecting endothelial cells...


    pub_type: 杂志文章


    authors: Chapagain ML,Verma S,Mercier F,Yanagihara R,Nerurkar VR

    更新日期:2007-07-20 00:00:00

  • Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization.

    abstract::RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...


    pub_type: 杂志文章


    authors: Navarro JA,Darós JA,Flores R

    更新日期:1999-01-05 00:00:00

  • Loss of dimerisation of the nonstructural protein NS1 of Kunjin virus delays viral replication and reduces virulence in mice, but still allows secretion of NS1.

    abstract::The flavivirus nonstructural protein NS1 has been implicated in viral RNA replication, although its precise role has not been identified. In its native state NS1 exists as a heat labile homodimer that is thought to be required for NS1 function and secretion. However, we have recently identified a cDNA clone of KUN vir...


    pub_type: 杂志文章


    authors: Hall RA,Khromykh AA,Mackenzie JM,Scherret JH,Khromykh TI,Mackenzie JS

    更新日期:1999-11-10 00:00:00

  • Immunization with dissociated neuraminidase, matrix, and nucleoproteins from influenza A virus eliminates cognate help and antigenic competition.

    abstract::When hemagglutinin (HA) and neuraminidase (NA) are presented together on an intact influenza virus particle, the antigens are competitive, with HA dominant over NA in T- and B-cell priming. Immunization with mixtures of purified HA and NA eliminates antigenic competition between HA and NA, as well as between N1-N2 NA ...


    pub_type: 杂志文章


    authors: Johansson BE,Kilbourne ED

    更新日期:1996-11-01 00:00:00

  • Crystalline cowpea mosaic virus.

    abstract::Crystals of Cowpea mosaic virus displaying a high degree of order have been grown in two different forms. Hexagonal crystals, space group P6(1)22 or P6(5)22 with a = 450 A and c = 1038 A containing six virus particles per cell, have been grown in sodium citrate buffer at pH 4.9. Each particle has four nearest neighbor...


    pub_type: 杂志文章


    authors: White JM,Johnson JE

    更新日期:1980-02-01 00:00:00

  • Cloning of SV40 genomes from human brain tumors.

    abstract::From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...


    pub_type: 杂志文章


    authors: Krieg P,Scherer G

    更新日期:1984-10-30 00:00:00

  • Avian-virus-like receptor specificity of the hemagglutinin impedes influenza virus replication in cultures of human airway epithelium.

    abstract::A non-optimal receptor-binding specificity of avian influenza viruses is believed to hamper their replication in humans; however, the magnitude of this restriction remains undefined. Here we generated recombinant viruses, R1 and R2, that differed solely by two amino acids in the receptor-binding site of their hemagglu...


    pub_type: 杂志文章


    authors: Matrosovich M,Matrosovich T,Uhlendorff J,Garten W,Klenk HD

    更新日期:2007-05-10 00:00:00

  • Activation of human immunodeficiency virus type 1 provirus in T-cells and macrophages is associated with induction of inducer-specific NF-kappa B binding proteins.

    abstract::We have analyzed the limiting factors involved in the induction of human immunodeficiency virus type 1 (HIV-1) provirus expression by tumor necrosis factor-alpha (TNF-alpha), phorbol-12-myristate-13-acetate (PMA), and bryostatin-1 in T-cells (ACH-2) and monocytes (U1). We have demonstrated that, while there is a corre...


    pub_type: 杂志文章


    authors: Vlach J,Pitha PM

    更新日期:1992-03-01 00:00:00

  • Latent infection can be established with drastically restricted transcription and replication of the HSV-1 genome.

    abstract::Viral functions essential for the establishment of latent infection in murine sensory neurons in vivo were investigated by employing a herpes simplex virus type 1 (HSV-1) variant (KD6/B11) deleted for expression of the ICP4 gene and therefore unable to replicate. Since the viral DNA persisted in these cells, the laten...


    pub_type: 杂志文章


    authors: Sedarati F,Margolis TP,Stevens JG

    更新日期:1993-02-01 00:00:00

  • In vitro differentiation of monocytoid THP-1 cells affects their permissiveness for HIV strains: a model system for studying the cellular basis of HIV differential tropism.

    abstract::The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Vir...


    pub_type: 杂志文章


    authors: Meylan PR,Spina CA,Richman DD,Kornbluth RS

    更新日期:1993-03-01 00:00:00

  • Functional dissection of the ie2 gene product of the baculovirus Autographa californica nuclear polyhedrosis virus.

    abstract::The PstI-N genomic fragment of Autographa californica nuclear polyhedrosis virus (AcNPV) encodes an immediate-early protein, IE2, that functions as a promiscuous transactivator of other early viral promoters and heterologous promoters both in vivo and in vitro. IE2 contains several sequence motifs that are common to t...


    pub_type: 杂志文章


    authors: Yoo S,Guarino LA

    更新日期:1994-07-01 00:00:00

  • Specific functions of the Rep and Rep׳ proteins of porcine circovirus during copy-release and rolling-circle DNA replication.

    abstract::The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates...


    pub_type: 杂志文章


    authors: Cheung AK

    更新日期:2015-07-01 00:00:00

  • The complete genomic sequence of lytic bacteriophage gh-1 infecting Pseudomonas putida--evidence for close relationship to the T7 group.

    abstract::The genome of the lytic Pseudomonas putida bacteriophage gh-1 is linear double-stranded DNA containing 37,359 bp with 216-bp direct terminal repeats. Like other members of the T7 group, the gh-1 genome contains regions of high homology to T7 interspersed with nonhomologous regions that contain small open reading frame...


    pub_type: 杂志文章


    authors: Kovalyova IV,Kropinski AM

    更新日期:2003-07-05 00:00:00

  • Transcript mapping of Abutilon mosaic virus, a geminivirus.

    abstract::The transcripts of the DNA of Abutilon mosaic virus, a geminivirus with a bipartite genome (DNA A and DNA B), were characterized by Northern blot hybridization, S1 nuclease assay, primer extension analysis, and sequencing of the 3' termini of cDNA clones. It was shown that transcription is bidirectional and that the t...


    pub_type: 杂志文章


    authors: Frischmuth S,Frischmuth T,Jeske H

    更新日期:1991-12-01 00:00:00

  • Use of Cre/loxP recombination to swap cell binding motifs on the adenoviral capsid protein IX.

    abstract::We used Cre/loxP recombination to swap targeting ligands present on the adenoviral capsid protein IX (pIX). A loxP-flanked sequence encoding poly-lysine (pK-binds heparan sulfate proteoglycans) was engineered onto the 3'-terminus of pIX, and the resulting fusion protein allowed for routine virus propagation. Growth of...


    pub_type: 杂志文章


    authors: Poulin KL,Tong G,Vorobyova O,Pool M,Kothary R,Parks RJ

    更新日期:2011-11-25 00:00:00

  • Protective role of Indoleamine 2,3 dioxygenase in Respiratory Syncytial Virus associated immune response in airway epithelial cells.

    abstract::RSV is a major cause of severe lower respiratory infection in infants and young children. With no vaccine yet available, it is important to clarify mechanisms of disease pathogenesis. Since indoleamine-2,3-dioxygenase (IDO) is an immunomodulatory enzyme and is upregulated with RSV infection, we studied it in vivo duri...


    pub_type: 杂志文章


    authors: Rajan D,Chinnadurai R,O'Keefe EL,Boyoglu-Barnum S,Todd SO,Hartert TV,Galipeau J,Anderson LJ

    更新日期:2017-12-01 00:00:00

  • Characterization of bovine adenovirus type 3 E1 proteins and isolation of E1-expressing cell lines.

    abstract::Like human adenovirus type 5 (HAV5), bovine adenovirus type 3 (BAV3) early region 1 (E1) consists of E1A and E1B transcriptional units. In order to characterize BAV3 E1 proteins and to isolate a cell line of bovine origin that expresses BAV3 E1, polyclonal antibodies specific to E1A, E1B-157R, and E1B-420R were raised...


    pub_type: 杂志文章


    authors: van Olphen AL,Tikoo SK,Mittal SK

    更新日期:2002-03-30 00:00:00

  • Self-inactivating lentiviral vectors with U3 and U5 modifications.

    abstract::Lentiviral vectors have gained much attention in recent years mainly because they integrate into nondividing host-cell genomes. For clinical applications, a safe and efficient lentiviral vector system is required. Previously, we have established a human immunodeficiency virus type 1 (HIV-1)-derived three-plasmid lenti...


    pub_type: 杂志文章


    authors: Iwakuma T,Cui Y,Chang LJ

    更新日期:1999-08-15 00:00:00

  • The most frequent naturally occurring length polymorphism in the HIV-1 LTR has little effect on proviral transcription and viral replication.

    abstract::About 38% of primary HIV-1 long terminal repeats (LTRs) contain an insertion (consensus: 5prime prime or minute-ACYGCTGA-3prime prime or minute), termed the most frequent naturally occurring length polymorphism (MFNLP). The MFNLP binds several transcription factors and might affect HIV-1 replication and disease progre...


    pub_type: 杂志文章


    authors: Hiebenthal-Millow K,Kirchhoff F

    更新日期:2002-01-05 00:00:00

  • Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection.

    abstract::Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber mo...


    pub_type: 杂志文章


    authors: Yan Y,Zhang B,Hou W,Lin H,Rebetz J,Hong SS,Wang Y,Ran L,Fan X

    更新日期:2017-05-01 00:00:00

  • Experimental confirmation of a hepatitis B virus (HBV) epsilon-like bulge-and-loop structure in avian HBV RNA encapsidation signals.

    abstract::Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...


    pub_type: 杂志文章


    authors: Beck J,Bartos H,Nassal M

    更新日期:1997-01-20 00:00:00

  • The endogenous langur type D retrovirus PO-1-Lu and its exogenous counterparts in macaque and langur monkeys.

    abstract::PO-1-Lu, the endogenous type D retrovirus of langurs (Trachypithecus obscurus) has previously been considered a progenitor to the prototype type D retrovirus, Mason Pfizer monkey virus (M-PMV/SRV-3), that became established in macaque monkeys (Macaca spp.) following a zoonosis. This study reevaluates this hypothesis t...


    pub_type: 杂志文章


    authors: Sommerfelt MA,Harkestad N,Hunter E

    更新日期:2003-10-25 00:00:00

  • Equine schlafen 11 restricts the production of equine infectious anemia virus via a codon usage-dependent mechanism.

    abstract::Human schlafen11 is a novel restriction factor for HIV-1 based on bias regarding relative synonymous codon usage (RSCU). Here, we report the cloning of equine schlafen11 (eSLFN11) and the characteristics of its role in restricting the production of equine infectious anemia virus (EIAV), a retrovirus similar to HIV-1. ...


    pub_type: 杂志文章


    authors: Lin YZ,Sun LK,Zhu DT,Hu Z,Wang XF,Du C,Wang YH,Wang XJ,Zhou JH

    更新日期:2016-08-01 00:00:00

  • Functionality of host proteins in Cucumber mosaic virus replication: GAPDH is obligatory to promote interaction between replication-associated proteins.

    abstract::Here, we evaluated the role of two host proteins, a Bromo domain containing RNA binding protein (BRP1) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), in the replication of Cucumber mosaic virus (CMV). LC-MS/MS analysis of host/viral proteins pull down against BRP1 from CMV-infected plants co-infiltrated with BR...


    pub_type: 杂志文章


    authors: Chaturvedi S,Seo JK,Rao AL

    更新日期:2016-07-01 00:00:00

  • Extensive immune-mediated hippocampal damage in mice surviving infection with neuroadapted Sindbis virus.

    abstract::Viral infections of the central nervous system and immune responses to these infections cause a variety of neurological diseases. Infection of weanling mice with Sindbis virus causes acute nonfatal encephalomyelitis followed by clearance of infectious virus, but persistence of viral RNA. Infection with a neuroadapted ...


    pub_type: 杂志文章


    authors: Kimura T,Griffin DE

    更新日期:2003-06-20 00:00:00

  • The transmembrane domains of the EBV-encoded latent membrane protein 1 (LMP1) variant CAO regulate enhanced signalling activity.

    abstract::Sequence variants of the Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP1) have been reported in association with EBV-linked malignancies but little is known about their effects on signalling pathways and phenotype. We have examined the ability of the nasopharyngeal carcinoma (NPC)-derived variant, CAO...


    pub_type: 杂志文章


    authors: Blake SM,Eliopoulos AG,Dawson CW,Young LS

    更新日期:2001-04-10 00:00:00