当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > HuR, a protein implicated in oncogene and growth factor mRNA decay, binds to the 3' ends of hepatitis C virus RNA of both polarities.

HuR, a protein implicated in oncogene and growth factor mRNA decay, binds to the 3' ends of hepatitis C virus RNA of both polarities.

Abstract：

:To identify cellular factors that interact with hepatitis C virus RNA, cellular extracts were subjected to UV cross-linking to radiolabeled RNAs corresponding to the hepatitis C virus 5' and 3' untranslated regions of positive and negative polarities. Our results demonstrate that the U-rich region of the hepatitis C virus 3' untranslated region of the positive RNA strand is a hot spot for cellular RNA binding proteins. Two of these proteins were identified as the ELAV-like HuR protein and hnRNP C. Interestingly, HuR and hnRNP C also interacted with the 3' end of the RNA representing the negative strand of the HCV genome. The binding of HuR and hnRNP C to the 3' ends of the HCV RNAs of both negative and positive polarities suggests that HuR and hnRNP C may be involved in the transcription of the HCV RNA genome. Alternatively, they act by protecting the HCV RNAs from premature degradation by binding to their 3' ends. However, we were unable to demonstrate an effect on HCV RNA stability by the HuR protein. These interactions may be necessary for the establishment of chronic active infections that may develop into cirrhosis or hepatocellular carcinoma.

journal_name

Virology

journal_title

Virology

authors

Spångberg K,Wiklund L,Schwartz S

doi

10.1006/viro.2000.0461

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

378-90

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(00)90461-6

journal_volume

274

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Interferon-alpha-induced gene expression: evidence for a selective effect of ouabain on activation of the ISGF3 transcription complex.

    abstract：:Binding of interferons (IFNs) to their cell surface receptors stimulates rapid translocation of cytoplasmic proteins to the nucleus and the expression of a variety of cellular genes within minutes. Translocated proteins subsequently bind to the interferon-stimulated response element (ISRE) located in the promoters of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)91207-b

    authors： Nakagawa Y,Petricoin EF 3rd,Akai H,Grimley PM,Rupp B,Larner AC

    更新日期：1992-09-01 00:00:00

  • Study of genetic reassortment between two human rotaviruses.

    abstract：:Eight different human rotavirus strains were adapted to replicate in cell culture. In vitro, rotavirus genetic reassortment has been performed between two virus strains, one with a long pattern of segmented genomic RNAs and the other with a short pattern in the electrophoretic mobility of genomic RNAs. This genetic re...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90381-7

    authors： Garbarg-Chenon A,Bricout F,Nicolas JC

    更新日期：1984-12-01 00:00:00

  • Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.

    abstract：:Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.03.020

    authors： González-Hernández M,Müller A,Hoenen T,Hoffmann M,Pöhlmann S

    更新日期：2019-06-01 00:00:00

  • In vivo infection of IgG-containing cells by Jembrana disease virus during acute infection.

    abstract：:Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cell...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.07.027

    authors： Desport M,Tenaya IW,McLachlan A,McNab TJ,Rachmat J,Hartaningsih N,Wilcox GE

    更新日期：2009-10-25 00:00:00

  • Sendai virus defective-interfering genomes and the activation of interferon-beta.

    abstract：:The ability of some Sendai virus stocks to strongly activate IFNbeta has long been known to be associated with defective-interfering (DI) genomes. We have compared SeV stocks containing various copyback and internal deletion DI genomes (and those containing only nondefective (ND) genomes) for their ability to activate...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.03.022

    authors： Strahle L,Garcin D,Kolakofsky D

    更新日期：2006-07-20 00:00:00

  • In vivo analysis of the cell cycle dependent association of the bovine papillomavirus E2 protein and ChlR1.

    abstract：:It has been shown that the genomes of episomally maintained DNA viruses are tethered to host cell chromosomes during cell division, facilitating maintenance in dividing cells. The papillomavirus E2 protein serves this mechanism of viral genome persistence by simultaneously associating with chromatin and the viral geno...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.03.015

    authors： Feeney KM,Saade A,Okrasa K,Parish JL

    更新日期：2011-05-25 00:00:00

  • Characterization of the Rep78/adeno-associated virus complex.

    abstract：:Adeno-associated virus (AAV) replication proteins Rep78 and Rep68 play major roles in the life cycle of AAV. We have recently provided in vivo evidence for the existence of a covalent association between Rep78 and virion single-stranded (ss) AAV DNA (K. M. R. Prasad and J.P. Trempe(1995) Virology 214, 360-370). In thi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8431

    authors： Prasad KM,Zhou C,Trempe JP

    更新日期：1997-03-03 00:00:00

  • Identification of a new neutralization epitope on VP7 of human serotype 2 rotavirus and evidence for electropherotype differences caused by single nucleotide substitutions.

    abstract：:Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1601

    authors： Dunn SJ,Ward RL,McNeal MM,Cross TL,Greenberg HB

    更新日期：1993-11-01 00:00:00

  • Molecular characterisation of a novel pathogenic avipoxvirus from the Australian magpie (Gymnorhina tibicen).

    abstract：:Avipoxviruses are significant pathogens infecting a wide range of wild and domestic bird species globally. Here, we describe a novel genome sequence of magpiepox virus (MPPV) isolated from an Australian magpie. In the present study, histopathologically confirmed cutaneous pox lesions were used for transmission electro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.11.005

    authors： Sarker S,Batinovic S,Talukder S,Das S,Park F,Petrovski S,Forwood JK,Helbig KJ,Raidal SR

    更新日期：2020-01-15 00:00:00

  • Mutational analysis of the translational signal in the human cytomegalovirus gpUL4 (gp48) transcript leader by retroviral infection.

    abstract：:A short upstream open reading frame (uORF2) in the human cytomegalovirus (CMV) gpUL4 (gp48) transcript leader is conserved among CMV strains and inhibits translation of a downstream cistron. Remarkably, this inhibitory effect depends on the amino acid coding information of uORF2, at least in transient transfection ass...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1630

    authors： Cao J,Geballe AP

    更新日期：1994-11-15 00:00:00

  • RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs.

    abstract：:Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various step...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.08.026

    authors： Huang F,Zhang J,Zhang Y,Geng G,Liang J,Li Y,Chen J,Liu C,Zhang H

    更新日期：2015-12-01 00:00:00

  • Coexpression of the adenovirus 12 E1B 55 kDa oncoprotein and cellular tumor suppressor p53 is sufficient to induce metaphase fragility of the human RNU2 locus.

    abstract：:Adenovirus 12 (Ad12), but not adenovirus 2 or 5, induces metaphase chromosome fragility at four specific loci in humans: RNU1, RNU2, PSU1, and RN5S. As each of these sites corresponds to a tandemly repeated multigene family encoding a small, abundant structural RNA, we proposed that Ad12 hinders metaphase chromatin co...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9512

    authors： Liao D,Yu A,Weiner AM

    更新日期：1999-02-01 00:00:00

  • The v-Rel oncoprotein complexes with new Rel- and RelA-related proteins in transformed cells.

    abstract：:The v-Rel oncoprotein of the Rev-T retrovirus interacts with a number of cellular proteins in transformed chicken spleen cells including p40/I kappa B alpha, p68c-Rel, hsc70, and the p124 and p115 precursors for the p50 and p52 subunits of NF kappa B. Here we report that v-Rel associates with at least three other cell...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1095

    authors： Xu X,Gélinas C

    更新日期：1995-03-10 00:00:00

  • Domain- and nucleotide-specific Rev response element regulation of feline immunodeficiency virus production.

    abstract：:Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analy...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.04.009

    authors： Na H,Huisman W,Ellestad KK,Phillips TR,Power C

    更新日期：2010-09-01 00:00:00

  • Expression profiles of TCRbeta and CD8alpha mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp.

    abstract：:Our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. In the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express CD8alpha and TCRbeta...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.01.019

    authors： Somamoto T,Yoshiura Y,Sato A,Nakao M,Nakanishi T,Okamoto N,Ototake M

    更新日期：2006-05-10 00:00:00

  • Role of Tobacco vein banding mosaic virus 3'-UTR on virus systemic infection in tobacco.

    abstract：:To investigate the role of Tobacco vein banding mosaic virus (TVBMV) 3'-UTR in virus systemic infection, three types of deletions were introduced into TVBMV infectious clone pCaTVBMV-GFP. Mutants with deletions at the nucleotide position 8-42, 43-141, or 163-174 in the 3'-UTR failed to cause systemic infection in N. b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.11.001

    authors： Wang Y,Yang ZY,Tian YP,Geng C,Yuan XF,Li XD

    更新日期：2019-01-15 00:00:00

  • Human papillomavirus 16 E6 variants differ in their dysregulation of human keratinocyte differentiation and apoptosis.

    abstract：:L83V-related variants of human papillomavirus (HPV) 16 E6, exemplified by the Asian-American variant Q14H/H78Y/L83V, were shown to be more prevalent than E6 prototype in progressing lesions and cervical cancer. We evaluated functions relevant to carcinogenesis for the E6 variants L83V, R10/L83V and Q14H/H78Y/L83V as w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.09.036

    authors： Zehbe I,Richard C,DeCarlo CA,Shai A,Lambert PF,Lichtig H,Tommasino M,Sherman L

    更新日期：2009-01-05 00:00:00

  • Sulfated polymers inhibit the interaction of human cytomegalovirus with cell surface heparan sulfate.

    abstract：:Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, heparin) and copolymers of acrylic acid with vinylalcohol sulfate have proved to be potent inhibitors of human cytomegalovirus (CMV) infectivity in vitro. Sulfated alpha-cyclodextrins are only weak inhibitors of CMV. A close correlation was found...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90680-n

    authors： Neyts J,Snoeck R,Schols D,Balzarini J,Esko JD,Van Schepdael A,De Clercq E

    更新日期：1992-07-01 00:00:00

  • Ski protein levels increase during in vitro progression of HPV16-immortalized human keratinocytes and in cervical cancer.

    abstract：:We compared the levels of the Ski oncoprotein, an inhibitor of transforming growth factor-beta (TGF-β) signaling, in normal human keratinocytes (HKc), HPV16 immortalized HKc (HKc/HPV16), and differentiation resistant HKc/HPV16 (HKc/DR) in the absence and presence of TGF-β. Steady-state Ski protein levels increased in ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.05.039

    authors： Chen Y,Pirisi L,Creek KE

    更新日期：2013-09-01 00:00:00

  • A new and distinct species in the genus Caulimovirus exists as an endogenous plant pararetroviral sequence in its host, Dahlia variabilis.

    abstract：:Viruses in certain genera in family Caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (EPRV). However, members of the genus Caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected ce...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.03.003

    authors： Pahalawatta V,Druffel K,Pappu H

    更新日期：2008-07-05 00:00:00

  • Molecular cloning of a novel human papillomarvirus (type 58) from an invasive cervical carcinoma.

    abstract：:A novel human papillomavirus type (HPV) was cloned from an invasive cervical carcinoma. The viral clone showed no homology with other known prototypes of HPV (HPV-1 through HPV-57), except HPV-33 by Southern blot analysis under stringent conditions. It showed less than 20% homology to HPV-33 by reassociation kinetic a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90560-e

    authors： Matsukura T,Sugase M

    更新日期：1990-08-01 00:00:00

  • Specific functions of the Rep and Rep׳ proteins of porcine circovirus during copy-release and rolling-circle DNA replication.

    abstract：:The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.01.004

    authors： Cheung AK

    更新日期：2015-07-01 00:00:00

  • Analysis of nucleotides 13-96 of the human parainfluenza virus type 3 antigenomic promoter reveals positive- and negative-acting replication elements.

    abstract：:During replication of human parainfluenza virus type 3 (HPIV3), the 96-nucleotide antigenomic promoter (AGP) of HPIV3 directs the synthesis of genomic RNA. Previous work showed that nucleotides 1-12 were critical in promoting replication of an HPIV3 minireplicon, but nucleotides 13-96 were not investigated. In this st...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.08.003

    authors： Gander JR,Schwan LM,Hoffman MA

    更新日期：2011-10-25 00:00:00

  • Preferential replication of FIV in activated CD4(+)CD25(+)T cells independent of cellular proliferation.

    abstract：:Studies attempting to identify reservoirs of HIV-1 latency have documented that the virus persists as both a latent and productive infection in subsets of CD4(+) cells. Reports regarding establishment of a stable HIV-1 infection in quiescent T cells in vitro, however, are controversial. In the present study, we invest...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.01.014

    authors： Joshi A,Vahlenkamp TW,Garg H,Tompkins WA,Tompkins MB

    更新日期：2004-04-10 00:00:00

  • An analysis of the requirements for human cytomegalovirus oriLyt-dependent DNA synthesis in the presence of the herpes simplex virus type 1 replication fork proteins.

    abstract：:Activation of the human cytomegalovirus (HCMV) origin of replication (oriLyt) was previously demonstrated in transient transfection assays in permissive human fetal fibroblasts and nonpermissive Vero cells, and shown to require six viral proteins that function at the replication fork plus a number of HCMV products tha...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00005-9

    authors： Reid GG,Ellsmore V,Stow ND

    更新日期：2003-04-10 00:00:00

  • Recovery of structurally intact and infectious poliovirus type 1 from HeLa cells during receptor-mediated endocytosis.

    abstract：:Poliovirus type 1 enters HeLa cells by receptor-mediated endocytosis as an intact virus. Up to 30 min after adsorption complete virus particles still containing VP4 and sedimenting with 156 S could be recovered from the cells. These virus particles were N-antigenic and infectious. Thirty minutes after adsorption the r...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90286-9

    authors： Willingmann P,Barnert H,Zeichhardt H,Habermehl KO

    更新日期：1989-02-01 00:00:00

  • Mitigation of variation observed in a peripheral blood mononuclear cell (PBMC) based HIV-1 neutralization assay by donor cell pooling.

    abstract：:Cultured primary peripheral blood mononuclear cells (PBMC) represent a potentially physiologic in vitro model of HIV-1 infection, but assessment of antibody-mediated HIV-1 neutralization using PBMC has been hindered by donor variability and lack of a sustainable individual PBMC source. To advance this model for HIV va...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.09.014

    authors： Wieczorek L,Brown BK,Delsarto Macedo C,Wesberry-Schmierer M,Ngauy V,Rosa Borges A,Michael NL,Marovich MA,Montefiori DC,Polonis VR

    更新日期：2013-12-01 00:00:00

  • Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization.

    abstract：:RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9497

    authors： Navarro JA,Darós JA,Flores R

    更新日期：1999-01-05 00:00:00

  • Molecular characterization of human immunodeficiency virus (HIV)-1 and -2 in individuals from guinea-bissau with single or dual infections: predominance of a distinct HIV-1 subtype A/G recombinant in West Africa.

    abstract：:Guinea-Bissau in West Africa has the highest prevalence of human immunodeficiency virus (HIV)-2 infection in the world, but recently the HIV-1 prevalence increased rapidly with the subsequent appearance of HIV-1 and HIV-2 dual infections. Information about the genetic subtypes of HIV in the region is limited. Therefor...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9867

    authors： Andersson S,Norrgren H,Dias F,Biberfeld G,Albert J

    更新日期：1999-09-30 00:00:00

  • A collaborative report: rhinoviruses--extension of the numbering system from 89 to 100.

    abstract：:To define the number of rhinovirus serotypes, cross neutralization tests and characterization studies were completed on 25 candidate prototype rhinoviruses submitted to a third phase of a collaborative program. Based on the results, 11 distinct prototype strains were designated and the numbering system was extended to...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90367-9

    authors： Hamparian VV,Colonno RJ,Cooney MK,Dick EC,Gwaltney JM Jr,Hughes JH,Jordan WS Jr,Kapikian AZ,Mogabgab WJ,Monto A

    更新日期：1987-07-01 00:00:00