Abstract:
:Poliovirus type 1 enters HeLa cells by receptor-mediated endocytosis as an intact virus. Up to 30 min after adsorption complete virus particles still containing VP4 and sedimenting with 156 S could be recovered from the cells. These virus particles were N-antigenic and infectious. Thirty minutes after adsorption the recovery of intact and infectious virus decreased. This decrease presumably reflects viral uncoating in the acidic endosomes and/or lysosomes because virus particles could be localized in endosomes at this time. The direct involvement of clathrin-coated structures in the endocytosis of poliovirus has been deduced from the enclosure of poliovirus in coated vesicles at 10 min after adsorption. At this time intact and infectious virus could be recovered only after the coated vesicles were disrupted by treatment with 0.5 M Tris at pH 7.0.
journal_name
Virologyjournal_title
Virologyauthors
Willingmann P,Barnert H,Zeichhardt H,Habermehl KOdoi
10.1016/0042-6822(89)90286-9subject
Has Abstractpub_date
1989-02-01 00:00:00pages
417-20issue
2eissn
0042-6822issn
1096-0341journal_volume
168pub_type
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