Abstract:
:The tobacco etch virus 27-kDa nuclear inclusion a (NIa) proteinase was expressed in Escherichia coli as a recombinant fusion protein containing a seven-histidine tag at the amino-terminus. Catalytically active and inactive (by virtue of a single amino acid change) forms of the proteinase were purified to homogeneity in a two-column chromatographic procedure. The active form of the proteinase was slowly converted to a lower molecular weight form, while the inactive form was not. This conversion was dilution independent and thought to be intramolecular. Isolation of the approximately 2-kDa peptide cleavage product and determination of its N-terminal amino acid sequence positioned the cleavage site 24 amino acids from the carboxy-terminus of the proteinase. A recombinant NIa proteinase lacking the C-terminal 24 amino acids was shown to possess limited activity. Kinetic analyses of cleavage of a synthetic peptide by the full-length or truncated proteinase were conducted and indicated that the Km of the truncated proteinase was approximately fourfold higher than that of the full-length form. The truncated proteinase was approximately one-twentieth as efficient in proteolysis of the test peptide substrate as the full-length form.
journal_name
Virologyjournal_title
Virologyauthors
Parks TD,Howard ED,Wolpert TJ,Arp DJ,Dougherty WGdoi
10.1006/viro.1995.1331subject
Has Abstractpub_date
1995-06-20 00:00:00pages
194-201issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(85)71331-1journal_volume
210pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Mycophenolic acid (MPA), an inhibitor of inosine monophosphate dehydrogenase, shows strong anti-HIV activity in vitro in both human peripheral blood CD4+ lymphocytes and macrophages, as well as established human cell lines. MPA shows its greatest antiviral effects during the early stages of HIV infection. By limiting ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1437
更新日期:1995-08-20 00:00:00
abstract::Although defensins are known to inhibit the replication of human influenza A virus (IAV) in vitro, their in vivo expression during IAV infection is not known. Here we investigated mRNA and protein expression of several beta-defensins in the airways of IAV infected mice. Expression of murine beta-defensin (MBD)-3 and -...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.07.024
更新日期:2008-10-10 00:00:00
abstract::Interleukin (IL)-15, is a cytokine that is important for the maintenance of long-lasting, high-avidity T cell response to invading pathogens and has, therefore, been used in vaccine and therapeutic platforms as an adjuvant. In addition to pure protein delivery, plasmids encoding the IL-15 gene have been utilized. Howe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.017
更新日期:2009-10-10 00:00:00
abstract::In this report, we present the DNA sequence and transcriptional characterization of a gene (IR5) that maps within each of the inverted repeat (IR) segments of the equine herpesvirus type 1 (EHV-1) genome. The IR5 open reading frame (ORF) is located within both IR sequences (nucleotides 9932-10,642 of the IR). DNA sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90525-t
更新日期:1992-06-01 00:00:00
abstract::The human immunodeficiency virus (HIV-1) causes various hematopoietic abnormalities, with thrombocytopenia (TP) occurring in 30% of infected individuals. In the present study, we aimed to determine whether HIV-1 in the bone marrow of TP patients can infect primary megakaryocytes in vitro, which may contribute to the d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0193
更新日期:2000-03-30 00:00:00
abstract::Hepatitis C virus (HCV) is the major causative agent of blood-borne non-A non-B hepatitis. The persistence of HCV infection is believed to reflect escape from the host immunosurveillance system by mutations in hypervariable region 1 (HVR1) of the envelope protein 2 (E2). Two envelope proteins of HCV, E1 and E2, have b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8516
更新日期:1997-04-28 00:00:00
abstract::The proteins encoded by open reading frame 3 (ORF3) of the umbraviruses pea enation mosaic virus-2 and tobacco mottle virus, like that of groundnut rosette virus, mediated the movement of viral RNA through the phloem of infected Nicotiana benthamiana or N. clevelandii plants when they were expressed from chimeric toba...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1078
更新日期:2001-09-30 00:00:00
abstract::The main route of small ruminant lentivirus dissemination is the ingestion of infected cells present in colostrum and milk from infected animals. However, whether only macrophages or other cell subtypes are involved in this transmission is unknown. We derived epithelial cell cultures, 100% cytokeratin positive, from m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9752
更新日期:1999-06-20 00:00:00
abstract::Purified preparations of the Helminthosporium victoriae 190S (Hv190S) virus contain two sedimenting components that differ in capsid structure. The slower sedimenting component (190S-1) contained p88 and p83 as the major capsid proteins; the faster component (190S-2) contained p88 and p78. Previous peptide-mapping stu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90520-y
更新日期:1992-06-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus origin-dependent DNA replication requires the core replication proteins plus K-Rta and K-bZIP. To determine which K-bZIP protein domains contribute to oriLyt-dependent DNA replication and facilitate suppression of K-Rta-mediated transcriptional activation, we generated a series ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.019
更新日期:2007-12-20 00:00:00
abstract::Covalently closed circular dimers of phage lambda DNA have been found in Escherichia coli infected with lambda. These dimers can be formed by either the lambda Red or Int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda O and P genes or by joining of the cohesive ends. Dimers...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90136-2
更新日期:1977-09-01 00:00:00
abstract::Electron microscopy and sequence analysis have suggested the presence of distinct morphological regions within the reovirus cell attachment protein sigma 1. Kinking of purified sigma 1 observed by electron microscopy and sequence-predicted flexibility profiles suggest the presence of potential flexible regions in the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1465
更新日期:1994-08-15 00:00:00
abstract::EBNA-1 is the sole virally specified protein required for replication of the Epstein-Barr virus latency origin and also modulates its own expression. Both of these functions are mediated through specific DNA binding. We created a series of amino acid substitutions across the previously identified DNA-binding domain of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1669
更新日期:1994-12-01 00:00:00
abstract::The bipartite geminiviruses bean golden mosaic virus (BGMV), cabbage leaf curl virus (CabLCV), and tomato golden mosaic virus (TGMV) exhibit differential tissue tropism in Nicotiana benthamiana. In systemically infected leaves, BGMV remains largely confined to vascular-associated cells (phloem-limited), whereas CabLCV...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1205
更新日期:2001-12-20 00:00:00
abstract::Molluscum contagiosum virus (MCV) is a human poxvirus that produces small, benign skin tumors primarily in young children and encodes proteins that modulate the host immune response. The MC51L, MC53L, and MC54L open reading frames of MCV have significant amino acid sequence similarities and related proteins are encode...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9676
更新日期:1999-05-10 00:00:00
abstract::Eight rhesus macaques inoculated with molecularly cloned SIVmac239 developed viremia and virus-binding antibodies, but only one (macaque 2D) developed neutralizing antibodies to the virus. Viremia persisted in macaque 2D even in the presence of neutralizing antibodies. Neutralizing antibodies in the plasma collected f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1390
更新日期:1993-08-01 00:00:00
abstract::The promotion of membrane fusion by the paramyxovirus hemagglutinin-neuraminidase (HN) and fusion (F) proteins requires that they be derived from homologous viruses, suggesting the possibility that the promotion of fusion requires a virus-specific communication between the two glycoprotein spikes. We have evaluated th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1278
更新日期:1995-06-01 00:00:00
abstract::Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.12.005
更新日期:2019-02-01 00:00:00
abstract::To better understand the evolution of the viral envelope glycoproteins (Env) in HIV-1 infected individuals who progress to AIDS maintaining an exclusive CCR5-using (R5) virus population, we cloned and sequenced the env gene of longitudinally obtained primary isolates. A shift in the electrostatic potential towards an ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.014
更新日期:2008-09-15 00:00:00
abstract::It is well established that the human subgroup C adenovirus type 5 (Ad5) E1B 55 kDa protein can regulate the activity and concentration of the cellular tumor suppressor, p53. However, the contribution(s) of these functions of the E1B protein to viral reproduction remains unclear. To investigate this issue, we examined...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.016
更新日期:2008-09-01 00:00:00
abstract::The P670 promoter of HPV16 directs transcription of the virus late genes in the differentiating epithelium. We found that CCAAT/enhancer binding protein beta (C/EBPbeta), a key transcription factor that induces the terminal differentiation of keratinocytes, enhanced the P670-driven transcription in transient reporter ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.025
更新日期:2006-03-01 00:00:00
abstract::Both the brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV) RNA-dependent RNA polymerases (RdRps) were found to recognize the BMV core subgenomic promoter in the same manner, requiring specific functional groups at positions -17, -14, -13, and -11 relative to the subgenomic initiation site (+1). For CCM...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9449
更新日期:1998-12-05 00:00:00
abstract::Within the family of Poxviridae, the entomopoxviruses are the most distant relatives of the more well-known and intensively studied orthopoxviruses (vaccinia and variola). The growth of the entomopoxvirus from Amsacta moorei (AmEPV) has been characterized in cell culture and compared to that of vaccinia virus (VV), th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1428
更新日期:1995-08-20 00:00:00
abstract::We produced the putative protease 3C of human rhinovirus 1A (HRV-1A), a minor group rhinovirus, by Escherichia coli expression of a segment of HRV-1A cDNA coding for 3A, 3B, 3C, and parts of 2C and 3D (delta 2C3ABC delta 3D). The protease 3C was expected to be processed by intramolecular Q-G cleavages from the virus-s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90429-f
更新日期:1991-10-01 00:00:00
abstract::Human hepatitis B virus (HBV) envelopes contain three distinct glycoproteins called L, M, and S HBsAg. Each is posttranslationally modified to contain N-linked oligosaccharides. N-linked oligosaccharides, after attachment to a polypeptide backbone, are processed by enzymes within the endoplasmic reticulum (ER). There ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.0038
更新日期:1995-11-10 00:00:00
abstract::The enveloped virions of two strains of a granulosis virus, a synergistic Hawaiian (GVH) and a nonsynergistic Oregonian (GVO) strain which infect the armyworm, Pseudaletia unipuncta, were liberated from their capsules with 0.02 N NaOH and purified by filtration through membrane filters. The envelopes were solubilized ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90439-2
更新日期:1979-04-15 00:00:00
abstract::Measles virus V protein represses genome replication through a poorly understood mechanism, which led us to investigate whether V protein might be an RNA-binding modulatory factor. Recombinant V protein, expressed from transfected HEp-2 cells or E. coli, formed protein-RNA complexes with poly-guanosine (poly-G) or pol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.018
更新日期:2006-04-25 00:00:00
abstract::Cytotoxic T-cell clones raised against X-31 (H3N2) influenza virus in C57BL/6 mice can be directed against an influenza A virus subtype specific determinant (1). A representative T-cell clone (A3.1) has been used in combination with a set of genetically typed recombinant viruses, to show that the A/PR/8/34 nucleoprote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90413-6
更新日期:1984-03-01 00:00:00
abstract::Mammalian orthoreoviruses (reoviruses) are highly useful models for studies of double-stranded RNA virus replication and pathogenesis. We previously developed a strategy to recover prototype reovirus strain T3D from cloned cDNAs transfected into murine L929 fibroblast cells. Here, we report the development of a second...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.037
更新日期:2010-03-15 00:00:00
abstract::Rice tungro bacilliform virus (RTBV) replication intermediates have been studied in rice plants infected with the virus. Unencapsidated virus-specific molecules were identified which had open circular, linear, supercoiled (SC), strong-stop, single-stranded, linear double-stranded hairpin, and double-stranded with sing...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1577
更新日期:1994-11-01 00:00:00