Polymerase substrate depletion: a novel strategy for inhibiting the replication of the human immunodeficiency virus.

abstract：:Pre-exposure prophylaxis (PrEP) strategies utilizing anti-retroviral drugs show considerable promise for HIV prevention. However there is insufficient pharmacokinetic (PK) data on drug concentrations required for protection at the relevant mucosal tissues where the infection is initiated. Here we evaluated the utility...

journal_title：Virology

authors： Veselinovic M,Yang KH,LeCureux J,Sykes C,Remling-Mulder L,Kashuba ADM,Akkina R

更新日期：2014-09-01 00:00:00

abstract：:In this study we show that macrophages (Mphi) are latently infected with murine cytomegalovirus (MCMV). After clearance of acute MCMV infection, the predominant form of chronic infection in Balb mice is latency rather than persistence. Peritoneal exudate cells (PECs) from latently infected Balb mice (3-9 months postin...

journal_title：Virology

authors： Pollock JL,Presti RM,Paetzold S,Virgin HW 4th

更新日期：1997-01-06 00:00:00

abstract：:The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Vir...

journal_title：Virology

authors： Meylan PR,Spina CA,Richman DD,Kornbluth RS

更新日期：1993-03-01 00:00:00

abstract：:Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis. As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV ...

journal_title：Virology

authors： Gunn BM,Jones JE,Shabman RS,Whitmore AC,Sarkar S,Blevins LK,Morrison TE,Heise MT

更新日期：2018-02-01 00:00:00

abstract：:We studied experimental canine oral papillomavirus (COPV) infection by in situ hybridization and immunohistochemistry of weekly biopsies. After 4 weeks, viral DNA in rete ridges suggested a keratinocyte stem cell target. Abundant viral DNA was seen in E4-positive cells only. E4 was predominantly cytoplasmic but also n...

journal_title：Virology

authors： Nicholls PK,Doorbar J,Moore RA,Peh W,Anderson DM,Stanley MA

更新日期：2001-05-25 00:00:00

abstract：:Hepatitis C virus (HCV) is efficient in the establishment of persistent infection. We have previously shown that HCV core protein inhibits T cell proliferation through its interaction with the complement receptor, gC1qR. Here we show that HCV core-induced inhibition of T cell proliferation involves a G(0)/G(1) cell cy...

journal_title：Virology

authors： Yao ZQ,Eisen-Vandervelde A,Ray S,Hahn YS

更新日期：2003-09-15 00:00:00

abstract：:The adenovirus E1A 243R oncoprotein encodes a potent transcription-repression function within the N-terminal 80 amino acids. Our proposed model of E1A repression predicts that E1A interacts with important cellular proteins on chromatin. Consistent with this idea, we report here that E1A proteins from in vivo formaldeh...

journal_title：Virology

authors： Green M,Panesar NK,Loewenstein PM

更新日期：2008-02-05 00:00:00

abstract：:Animal viruses profoundly modify the metabolism of lipids, the synthesis of new membranes, and membrane traffic. These alterations are related to the replication of viral genomes. Addition of oleic acid from the beginning of poliovirus infection inhibits the appearance of virus polypeptides at concentrations that do n...

journal_title：Virology

authors： Guinea R,Carrasco L

更新日期：1991-11-01 00:00:00

abstract：:Measles virus (MV) causes various responses including the induction of immune responses, transient immunosuppression and establishment of long-lasting immunity. To obtain a comprehensive view of the effects of MV infection on target cells, DNA microarray analyses of two different cell-types were performed. An epitheli...

journal_title：Virology

authors： Sato H,Honma R,Yoneda M,Miura R,Tsukiyama-Kohara K,Ikeda F,Seki T,Watanabe S,Kai C

更新日期：2008-06-05 00:00:00

abstract：:Epizootics of a natural varicella-like disease occur in populations of nonhuman primates. Several primate herpesviruses have been isolated from these epizootics, but the relatedness of these isolates to each other is not well-defined. In this study, we demonstrated that the restriction endonuclease (REn) profiles of f...

journal_title：Virology

authors： Gray WL,Gusick NJ

更新日期：1996-10-01 00:00:00

abstract：:The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography em...

journal_title：Virology

authors： Goldschmidts W,Luka J,Pearson GR

更新日期：1987-03-01 00:00:00

abstract：:Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional...

journal_title：Virology

authors： Larson ET,Eilers BJ,Reiter D,Ortmann AC,Young MJ,Lawrence CM

更新日期：2007-07-05 00:00:00

abstract：:Bacteriophage phi 29 DNA polymerase is sensitive to aphidicolin (APH). DNA polymerase of the APH-resistant mutant, APHr71, was more sensitive to phosphonoacetic acid and butylphenyldeoxyguanosine 5'triphosphate than the wild type. Nucleotide sequence analysis revealed a single transition of G at nucleotide 562 to A in...

journal_title：Virology

authors： Matsumoto K,Kim CI,Kobayashi H,Kanehiro H,Hirokawa H

更新日期：1990-09-01 00:00:00

abstract：:Thirteen viable insertion mutants of vaccinia virus have been constructed. These mutants, containing coding sequences of the herpes simplex virus thymidine kinase (HSV-TK) gene, were generated by marker transfer via in vivo recombination. The mutants were identified using a replica filter plating technique by in situ ...

journal_title：Virology

authors： Perkus ME,Panicali D,Mercer S,Paoletti E

更新日期：1986-07-30 00:00:00

abstract：:The subgroup II luteovirus barley yellow dwarf virus-RPV (BYDV-RPV) acts as a helper virus for a satellite RNA (satRPV RNA). The subgroup II luteovirus beet western yellows virus (BWYV) and the ST9-associated RNA (ST9a RNA), a BWYV-associated RNA that encodes a polymerase similar to those of subgroup I luteoviruses, w...

journal_title：Virology

authors： Rasochová L,Passmore BK,Falk BW,Miller WA

更新日期：1997-05-12 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) is a type B retrovirus that is unique from other retroviruses in having multiple "tissue specific" and "hormone inducible" promoters. This unique feature has lead to the increasing interest in studying the biology of MMTV replication with the ultimate goal of developing MMTV based ...

journal_title：Virology

authors： Rizvi TA,Ali J,Phillip PS,Ghazawi A,Jayanth P,Mustafa F

更新日期：2009-03-15 00:00:00

abstract：:Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in con...

journal_title：Virology

authors： Kelleher CA,Paterson RK,Dreyfus DH,Streib JE,Xu JW,Takase K,Jones JF,Gelfand EW

更新日期：1995-04-20 00:00:00

abstract：:Antibodies against the L protein of Sendai virus were made by immunizing rabbits with a synthetic peptide representing a carboxyl-terminal region of the protein predicted from the base sequence of its gene. These antibodies were used to localize the L protein in viral nucleocapsids by electron microscopy. Immunogold l...

journal_title：Virology

authors： Portner A,Murti KG,Morgan EM,Kingsbury DW

更新日期：1988-03-01 00:00:00

abstract：:To identify cellular factors that interact with hepatitis C virus RNA, cellular extracts were subjected to UV cross-linking to radiolabeled RNAs corresponding to the hepatitis C virus 5' and 3' untranslated regions of positive and negative polarities. Our results demonstrate that the U-rich region of the hepatitis C v...

journal_title：Virology

authors： Spångberg K,Wiklund L,Schwartz S

更新日期：2000-09-01 00:00:00

abstract：:We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...

journal_title：Virology

authors： Jourdan M,Jousset FX,Gervais M,Skory S,Bergoin M,Dumas B

更新日期：1990-11-01 00:00:00

abstract：:Human parainfluenza virus type 1 (hPIV1) and type 3 (hPIV3) initiate infection by sialic acid binding. Here, we investigated sialic acid linkage specificities for binding and infection of hPIV1 and hPIV3 by using sialic acid linkage-modified cells treated with sialidases or sialyltransferases. The hPIV1 is bound to on...

journal_title：Virology

authors： Fukushima K,Takahashi T,Ito S,Takaguchi M,Takano M,Kurebayashi Y,Oishi K,Minami A,Kato T,Park EY,Nishimura H,Takimoto T,Suzuki T

更新日期：2014-09-01 00:00:00

abstract：:In order to study the effect of nef gene expression on viral replication in monocytic cells, we established monocytic (U937 and THP-1) cell transfectants constitutively expressing the human immunodeficiency virus type 1 nef gene. We constructed a plasmid expressing the nef gene derived from an infectious clone, NL432,...

journal_title：Virology

authors： Tsunetsugu-Yokota Y,Matsuda S,Maekawa M,Saito T,Takemori T,Takebe Y

更新日期：1992-12-01 00:00:00

abstract：:The sequence of the 5'-end of the genome of dengue 2 (Jamaica genotype) virus has been previously reported (V. Deubel, R. M. Kinney, and D. W. Trent, 1986, Virology 155, 365-377). We have now cloned and sequenced the remaining 75% of the genomic RNA that encodes the nonstructural proteins. The complete genome is 10,72...

journal_title：Virology

authors： Deubel V,Kinney RM,Trent DW

更新日期：1988-07-01 00:00:00

abstract：:Circomics was coined to describe the combination of rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) and pyro-sequencing to investigate the genome structures of small circular DNAs. A batch procedure is described using 61 plant samples from Asia, South America and Central America whi...

journal_title：Virology

authors： Wyant PS,Strohmeier S,Schäfer B,Krenz B,Assunção IP,Lima GS,Jeske H

更新日期：2012-06-05 00:00:00

abstract：:The synthesis and processing of the nucleocapsid protein (pp60) of the JHM strain of murine coronaviruses were examined. Pulse-chase experiments showed that pp60 was synthesized initially as a protein of approximately 57,000 in molecular weight (p57). Immunoprecipitation using mouse anti-JHMV antiserum indicated that ...

journal_title：Virology

authors： Stohlman SA,Fleming JO,Patton CD,Lai MM

更新日期：1983-10-30 00:00:00

abstract：:Resting peripheral blood mononuclear cells (PBMC) or purified T-cells can be induced to proliferate when cocultured in vitro with fixed HTLV-infected T-cells. This process of HTLV-dependent cellular activation and induction of proliferation has been considered distinctive because of an apparent independence from conve...

journal_title：Virology

authors： Martin TC,Southern PJ

更新日期：1996-07-15 00:00:00

abstract：:Vitis vinifera cultivars Zinfandel-1A and Mission were found to harbor different grapevine yellow speckle viroid-1 (GYSVd-1) variants and characterized to define the relationship to yellow speckle (YS) and vein-banding (VB) diseases. Products from the left terminal (T1), pathogenic (P), and a portion of the central (C...

journal_title：Virology

authors： Szychowski JA,Credi R,Reanwarakorn K,Semancik JS

更新日期：1998-09-01 00:00:00

abstract：:We constructed a sensitive and quantitative assay system to examine human T-cell leukemia virus type I (HTLV-I) envelope (env) glycoprotein-mediated cell fusion in which T7 RNA polymerase in donor cells coexpressing env glycoproteins activates a reporter gene in recipient cells upon cell fusion. An efficient expressio...

journal_title：Virology

authors： Okuma K,Nakamura M,Nakano S,Niho Y,Matsuura Y

更新日期：1999-02-15 00:00:00

abstract：:Sera from 215 non-drug-injecting Toba and Mataco-Mataguayo pure Indians belonging to four communities in northern Argentina were examined using assays that allow differentiation between reactivities due to type-specific antigens of the human T-cell leukemia/lymphoma virus (HTLV). Three of these populations have very l...

journal_title：Virology

authors： Ferrer JF,Del Pino N,Esteban E,Sherman MP,Dube S,Dube DK,Basombrio MA,Pimentel E,Segovia A,Quirulas S

更新日期：1993-12-01 00:00:00

abstract：:Cell-to-cell and long-distance transport of some plant viruses requires coordinated action of three movement proteins encoded by triple gene block (TGB). The largest of TGB proteins, TGBp1, is a member of the superfamily I of DNA/RNA helicases and possesses a set of conserved helicase sequence motifs necessary for vir...

journal_title：Virology

authors： Kalinina NO,Rakitina DV,Solovyev AG,Schiemann J,Morozov SY

更新日期：2002-05-10 00:00:00