当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Role of V protein RNA binding in inhibition of measles virus minigenome replication.

Role of V protein RNA binding in inhibition of measles virus minigenome replication.

Abstract：

:Measles virus V protein represses genome replication through a poorly understood mechanism, which led us to investigate whether V protein might be an RNA-binding modulatory factor. Recombinant V protein, expressed from transfected HEp-2 cells or E. coli, formed protein-RNA complexes with poly-guanosine (poly-G) or poly-U linked to agarose beads. RNA binding was not exclusive to ribonucleotide homopolymers as complex formation between V protein and an RNA molecule equivalent to the 3' terminal 107 bases of the measles virus genome was observed with an electrophoretic mobility shift assay (EMSA). The interaction with poly-G was used to further examine the RNA binding properties of V demonstrating that protein-RNA complex formation was dependent upon the unique Cys-rich carboxy terminus, a region also required to induce maximal repression of minireplicon-encoded reporter gene expression in transient assays. Surprisingly, two mutant proteins that contained Cys-to-Ala substitutions in the C-terminus were found to retain their ability to bind poly-G binding and repress minireplicon reporter gene expression indicating that neither activity was dependent on the integrity of all 7 C-terminal Cys residues. Additional genetic analysis revealed that amino acids 238-266 were necessary for efficient RNA binding and overlapped with residues (238-278) required for maximal repression induced by the C-terminal domain. In addition, a 10 amino acid deletion was identified (residues 238-247) that blocked RNA binding and repression indicating that these two activities were related.

journal_name

Virology

journal_title

Virology

authors

Parks CL,Witko SE,Kotash C,Lin SL,Sidhu MS,Udem SA

doi

10.1016/j.virol.2005.12.018

subject

Has Abstract

pub_date

2006-04-25 00:00:00

pages

96-106

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(05)00827-5

journal_volume

348

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Activation of bovine immunodeficiency-like virus expression by bovine herpesvirus type 1.

    abstract：:Bovine immunodeficiency-like virus (BIV) is a recently identified lentivirus that infects cattle. The virus has structural and genetic similarities to human HIV. The present study demonstrates that BIV can be activated by bovine herpesvirus type 1 (BHV-1), a pathogen frequently associated with cattle diseases. Activat...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90489-c

    authors： Geng Y,Kashanchi F,Wood C

    更新日期：1992-04-01 00:00:00

  • Genetic rearrangements on the Chlorovirus genome that switch between hyaluronan synthesis and chitin synthesis.

    abstract：:Chlorella viruses or chloroviruses form polysaccharide fibers on the cell wall of host Chlorella cells after infection. Such polysaccharides are either hyaluronan synthesized by virus-encoded hyaluronan synthase (HAS) or chitin synthesized by viral chitin synthase (CHS). Some chloroviruses synthesize both hyaluronan (...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.07.026

    authors： Mohammed Ali AM,Kawasaki T,Yamada T

    更新日期：2005-11-10 00:00:00

  • Inefficient entry of vicriviroc-resistant HIV-1 via the inhibitor-CCR5 complex at low cell surface CCR5 densities.

    abstract：:HIV-1 variants resistant to small molecule CCR5 inhibitors such as vicriviroc (VVC) have modified Env complexes that can use both the inhibitor-bound and -free forms of the CCR5 co-receptor to enter target cells. However, entry via the inhibitor-CCR5 complex is inefficient in some, but not all, cell types, particularl...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.02.044

    authors： Pugach P,Ray N,Klasse PJ,Ketas TJ,Michael E,Doms RW,Lee B,Moore JP

    更新日期：2009-05-10 00:00:00

  • Terminal modifications of norovirus P domain resulted in a new type of subviral particles, the small P particles.

    abstract：:The protruding (P) domain of norovirus VP1 is responsible for immune recognition and host receptor interaction. Our previous studies have demonstrated that a modification of the ends of the P domain affects the conformation and/or function of the P protein. An expression of the P domain with or without the hinge, or w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.11.017

    authors： Tan M,Fang PA,Xia M,Chachiyo T,Jiang W,Jiang X

    更新日期：2011-02-20 00:00:00

  • Functional and molecular characterization of African swine fever virus mutants resistant to phosphonoacetic acid.

    abstract：:African swine fever virus (ASFV) growth and plaque formation were inhibited by phosphonoacetic acid (PAA) concentrations of 200 micrograms/ml or more. One spontaneous mutant and two mutants isolated from mutagenized virus were resistant to PAA inhibition and showed practically normal viral DNA synthesis in the presenc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.9953

    authors： Marques MI,Costa JV

    更新日期：1995-12-01 00:00:00

  • Virome characterization of Cryphonectria parasitica isolates from Azerbaijan unveiled a new mymonavirus and a putative new RNA virus unrelated to described viral sequences.

    abstract：:Cryphonectria parasitica, the causal agent of chestnut blight is controlled in Europe through natural spread of Cryphonectria hypovirus 1 (CHV1), a mycovirus able to induce hypovirulence to the host. In recent years C. parasitica was reported infecting Azerbaijani population of chestnut, but the presence of CHV1 still...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.10.008

    authors： Forgia M,Isgandarli E,Aghayeva DN,Huseynova I,Turina M

    更新日期：2021-01-15 00:00:00

  • Immunization with dissociated neuraminidase, matrix, and nucleoproteins from influenza A virus eliminates cognate help and antigenic competition.

    abstract：:When hemagglutinin (HA) and neuraminidase (NA) are presented together on an intact influenza virus particle, the antigens are competitive, with HA dominant over NA in T- and B-cell priming. Immunization with mixtures of purified HA and NA eliminates antigenic competition between HA and NA, as well as between N1-N2 NA ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0581

    authors： Johansson BE,Kilbourne ED

    更新日期：1996-11-01 00:00:00

  • Crystalline cowpea mosaic virus.

    abstract：:Crystals of Cowpea mosaic virus displaying a high degree of order have been grown in two different forms. Hexagonal crystals, space group P6(1)22 or P6(5)22 with a = 450 A and c = 1038 A containing six virus particles per cell, have been grown in sodium citrate buffer at pH 4.9. Each particle has four nearest neighbor...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(80)90513-9

    authors： White JM,Johnson JE

    更新日期：1980-02-01 00:00:00

  • HIV-1 integration is inhibited by stimulation of the VPAC2 neuroendocrine receptor.

    abstract：:Successful HIV-1 infection requires a number of specific stages leading to integration of the provirus. We previously suggested that members of the VPAC neuroendocrine receptor family may play a role in HIV-1 infection. We now show that stimulation of the VPAC2 receptor with specific agonists provides strong resistanc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.12.012

    authors： Bokaei PB,Ma XZ,Sakac D,Branch DR

    更新日期：2007-05-25 00:00:00

  • Construction and characterisation of a full-length infectious molecular clone from a fast replicating, X4-tropic HIV-1 CRF02.AG primary isolate.

    abstract：:Based on our previous analysis of HIV-1 isolates from Cameroon, we constructed a full-length infectious molecular clone from a primary isolate belonging to the CRF02.AG group of recombinant viruses which dominate the HIV-epidemic in West and Central Africa. The virus derived by transfection of the proviral clone pBD6-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00381-7

    authors： Tebit DM,Zekeng L,Kaptué L,Kräusslich HG,Herchenröder O

    更新日期：2003-09-01 00:00:00

  • 3Y1 rat cells are defective in processing of the envelope precursor protein of AKR virus.

    abstract：:Rat 3Y1 cells were infected by AKR virus through microinjection of molecularly cloned proviral DNA. Based on a strong immunofluorescence using anti-p30 as an antiserum a cell clone (RESA-2) was selected that had a high expression of viral antigens. Subsequent restriction analysis of its DNA revealed that the RESA-2 cl...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90361-6

    authors： van der Hoorn FA,Saris CJ,Bloemers HP

    更新日期：1983-01-30 00:00:00

  • Restriction of a Sindbis virus mutant in BHK cells and relief of the restriction by the addition of adenosine.

    abstract：:SV(PZF) is a mutant of Sindbis virus (SV) which we selected on the basis of its ability to replicate in mosquito cells treated with pyrazofurin (PZF), a drug which inhibits pyrimidine nucleotide biosynthesis (Lin et al., 2000, Virology 272, 61-71). Three mutations, A6627U, A7543U, and C7593A, were identified in the ns...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1230

    authors： Lin YH,Simmonds HA,Stollar V

    更新日期：2002-01-05 00:00:00

  • Analysis of human immunodeficiency virus type 1 promoter insertion in vivo.

    abstract：:Our study reports the occurrence of HIV-1 insertional activation events in vivo. Using a previously described PCR assay, small aliquots of broncho-alveolar lavage (BAL) cells obtained from AIDS patients were analyzed. Nine percent (5/54) of the aliquots contained proviral-host sequence transcripts indicating HIV-1 pro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1161

    authors： Raineri I,Solèr M,Senn HP

    更新日期：1995-04-01 00:00:00

  • The v-Rel oncoprotein complexes with new Rel- and RelA-related proteins in transformed cells.

    abstract：:The v-Rel oncoprotein of the Rev-T retrovirus interacts with a number of cellular proteins in transformed chicken spleen cells including p40/I kappa B alpha, p68c-Rel, hsc70, and the p124 and p115 precursors for the p50 and p52 subunits of NF kappa B. Here we report that v-Rel associates with at least three other cell...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1095

    authors： Xu X,Gélinas C

    更新日期：1995-03-10 00:00:00

  • A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus.

    abstract：:A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility. Comparison of the nucleotide sequences in the coat protein cistron of an aphid transmissible isolate (TVMV-AT) with that of T...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90389-9

    authors： Atreya CD,Raccah B,Pirone TP

    更新日期：1990-09-01 00:00:00

  • Structure of the antigen from Fiji disease virus particles eliciting antibodies specific to double-stranded polyribonucleotides.

    abstract：:Antisera with Fiji disease virus (FDV) protein-specific and double-stranded (ds) RNA-specific antibodies were prepared by injecting rabbits with highly purified preparations of FDV cores. Electron microscopic examination of negatively stained core preparations disclosed the presence of icosahedral particles, about 54 ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(79)90495-1

    authors： van der Lubbe JL,Hatta T,Francki RI

    更新日期：1979-06-01 00:00:00

  • Relationship of bovine herpesvirus 1 immediate-early, early, and late gene expression to host cellular gene transcription.

    abstract：:Bovine herpesvirus 1 (BHV-1) gene expression was examined by RNA blot hybridization using clones representing immediate-early, early, and late genes. An immediate-early protein gene probe hybridized with two transcripts, 3.4 and 5.8 kb, expressed by infected cells in the presence of cycloheximide (CH). During infectio...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90744-a

    authors： Seal BS,Whetstone CA,Zamb TJ,Bello LJ,Lawrence WC

    更新日期：1992-05-01 00:00:00

  • Higher selection pressure from antiretroviral drugs in vivo results in increased evolutionary distance in HIV-1 pol.

    abstract：:We investigated the effect of selection pressures on evolution of HIV-1 pol in 51 patients after switching to a new antiretroviral combination reverse transcriptase (RT) inhibitor therapy. Evolution of the protease (PR) and RT reading frames were analysed separately. Pairwise evolutionary distances (ED) were calculate...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9774

    authors： Günthard HF,Leigh-Brown AJ,D'Aquila RT,Johnson VA,Kuritzkes DR,Richman DD,Wong JK

    更新日期：1999-06-20 00:00:00

  • In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes.

    abstract：:Potato spindle tuber viroid (PSTVd) is a small non-protein-coding RNA pathogen that can induce disease symptoms in a variety of plant species. How PSTVd induces disease symptoms is a long standing question. It has been suggested that PSTVd-derived small RNAs (sRNAs) could direct RNA silencing of a targeted host gene(s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.12.019

    authors： Eamens AL,Smith NA,Dennis ES,Wassenegger M,Wang MB

    更新日期：2014-02-01 00:00:00

  • HIV-1 transgenic rats develop T cell abnormalities.

    abstract：:HIV-1 infection leads to impaired antigen-specific T cell proliferation, increased susceptibility of T cells to apoptosis, progressive impairment of T-helper 1 (Th1) responses, and altered maturation of HIV-1-specific memory cells. We have identified similar impairments in HIV-1 transgenic (Tg) rats. Tg rats developed...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.12.010

    authors： Reid W,Abdelwahab S,Sadowska M,Huso D,Neal A,Ahearn A,Bryant J,Gallo RC,Lewis GK,Reitz M

    更新日期：2004-03-30 00:00:00

  • Protection against polyoma virus-induced tumors is perforin-independent.

    abstract：:CD8 T cells are necessary for controlling tumors induced by mouse polyoma virus (PyV), but the effector mechanism(s) responsible have not been determined. We examined the PyV tumorigenicity in C57BL/6 mice mutated in Fas or carrying targeted disruptions in the perforin gene or in both TNF receptor type I and type II g...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.08.044

    authors： Byers AM,Hadley A,Lukacher AE

    更新日期：2007-02-20 00:00:00

  • Inhibition of the epidermal growth factor receptor by erlotinib prevents immortalization of human cervical cells by Human Papillomavirus type 16.

    abstract：:The Human Papillomavirus type-16 (HPV-16) E6 and E7 oncogenes are selectively retained and expressed in cervical carcinomas, and expression of E6 and E7 is sufficient to immortalize human cervical epithelial cells. Expression of the epidermal growth factor receptor (EGFR) is often increased in cervical dysplasia and c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.09.014

    authors： Woodworth CD,Diefendorf LP,Jette DF,Mohammed A,Moses MA,Searleman SA,Stevens DA,Wilton KM,Mondal S

    更新日期：2011-12-05 00:00:00

  • Molecular cloning of a novel human papillomavirus (type 60) from a plantar cyst with characteristic pathological changes.

    abstract：:In a plantar cyst composed of the wall of the squamous cell layer and the horny inner substance in the lower dermis, we found characteristic pathological changes, such as cytoplasmic eosinophilic inclusions and vacuolated structure, and, immunohistochemically, the papillomavirus capsid antigen. The human papillomaviru...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)91254-r

    authors： Matsukura T,Iwasaki T,Kawashima M

    更新日期：1992-09-01 00:00:00

  • HIV-1 Env glycoproteins from two series of primary isolates: replication phenotype and immunogenicity.

    abstract：:Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monome...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8445

    authors： Mustafa F,Richmond JF,Fernandez-Larsson R,Lu S,Fredriksson R,Fenyö EM,O'Connell M,Johnson E,Weng J,Santoro JC,Robinson HL

    更新日期：1997-03-03 00:00:00

  • Superinfection exclusion by Citrus tristeza virus does not correlate with the production of viral small RNAs.

    abstract：:Superinfection exclusion (SIE), a phenomenon in which a preexisting viral infection prevents a secondary infection with the same or closely related virus, has been described for different viruses, including important pathogens of humans, animals, and plants. Several mechanisms acting at various stages of the viral lif...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.08.031

    authors： Folimonova SY,Harper SJ,Leonard MT,Triplett EW,Shilts T

    更新日期：2014-11-01 00:00:00

  • The 5' noncoding region sequence of the Choristoneura biennis entomopoxvirus spheroidin gene functions as an efficient late promoter in the mammalian vaccinia expression system.

    abstract：:About 100 nucleotides of DNA sequence at the 5' noncoding region of the Choristoneura biennis entomopoxvirus spheroidin gene was chemically synthesized and inserted into a vaccinia expression vector, interrupting the vaccinia thymidine kinase gene. When the bacterial beta-galactosidase gene was introduced downstream o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90070-r

    authors： Pearson A,Richardson C,Yuen L

    更新日期：1991-02-01 00:00:00

  • Insights into the interaction between tRNA and primer binding site from characterization of a unique HIV-1 virus which stably maintains dual PBS complementary to tRNA(Gly) and tRNA(His).

    abstract：:Previously our laboratory constructed an HIV-1 which stably maintained a primer binding site (PBS) complementary to tRNA(His) by mutating the region of the provirus within U5 postulated to interact with the anticodon of tRNA(His) (J. Wakefield, S-M Kang, and C. D. Morrow, 1996, J. Virol, 70, 966-975). From the analysi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8837

    authors： Li Y,Zhang Z,Kang SM,Buescher JL,Morrow CD

    更新日期：1997-11-24 00:00:00

  • Retroviral infection of non-dividing cells: old and new perspectives.

    abstract：:The dependence of retroviral replication on cell proliferation was described as early as 1958, although different classes of retroviruses are able to infect non-dividing cells with different efficiencies. For example, the human immunodeficiency virus (HIV) and other lentiviruses infect most non-dividing cells nearly a...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2005.09.012

    authors： Yamashita M,Emerman M

    更新日期：2006-01-05 00:00:00

  • Endocytosis-mediated HIV-1 entry and its significance in the elusive behavior of the virus in astrocytes.

    abstract：:Astrocytes protect neurons but also evoke a proinflammatory response to injury and viral infections including HIV. We investigated the mechanism of HIV-1 infection in primary astrocytes, which showed minimal but productive viral infection independent of CXCR4. As with ectopic-CD4-expressing astrocytes, lysosomotropic ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.03.002

    authors： Chauhan A,Mehla R,Vijayakumar TS,Handy I

    更新日期：2014-05-01 00:00:00

  • Murine leukemia virus recombinants that use phosphate transporters for cell entry induce similar spongiform encephalomyelopathies in newborn mice.

    abstract：:Amphotropic Moloney-murine leukemia virus recombinants (Mo-AmphoV) induce a severe spongiform encephalomyelopathy in newborn mice. We show here that a coisogenic recombinant with a 10A1-MuLV host range (Mo-10A1V) also induces a neurodegenerative disease, clinically characterized by mild tremor and ataxia. Spongiform l...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9476

    authors： Münk C,Thomsen S,Stocking C,Löhler J

    更新日期：1998-12-20 00:00:00