Polyadenylation function and sequence variability of the long terminal repeats of the human endogenous retrovirus-like family RTVL-H.

abstract：:Coat protein-protected fragments of alfalfa mosaic virus RNA (AlMV-RNA) and tobacco streak virus RNA (TSV-RNA), which were isolated as described [D. Zuidema, M. F. A. Bierhuizen, B. J. C. Cornelissen, J. F. Bol, and E. M. J. Jaspars (1983)Virology, 125, 361-369], were tested for their ability to rebind AlMV coat prote...

journal_title：Virology

authors： Zuidema D,Cool RH,Jaspars EM

更新日期：1984-07-30 00:00:00

abstract：:Enterovirus 71 (EV71) is a major cause of hand-foot-and-mouth disease. EV71 infection occasionally associates with severe neurological sequelae such as brainstem encephalitis or poliovirus-like paralysis. We demonstrated that mouse-adapted strain increases infectivity, resulting in higher cytotoxicity of neuron cells ...

journal_title：Virology

authors： Huang SW,Wang YF,Yu CK,Su IJ,Wang JR

更新日期：2012-01-05 00:00:00

abstract：:Alphaviruses are mosquito-borne viruses that cause serious human and animal diseases. Previous studies demonstrated that a determinant within the nsP1/nsP2 cleavage domain of the virulent Sindbis AR86 virus played a key role in regulating adult mouse virulence without adversely affecting viral replication. Additional ...

journal_title：Virology

authors： Cruz CC,Suthar MS,Montgomery SA,Shabman R,Simmons J,Johnston RE,Morrison TE,Heise MT

更新日期：2010-03-30 00:00:00

abstract：:The pregenomic RNA (pgRNA) of hepadnaviruses serves a dual role: as mRNA for the core (C) and polymerase (P) synthesis and as an RNA template for viral genome replication. A question arises as to how these two roles are regulated. We hypothesized that the P protein could suppress translation of the pgRNA via its inter...

journal_title：Virology

authors： Ryu DK,Kim S,Ryu WS

更新日期：2008-03-30 00:00:00

abstract：:The flavivirus nonstructural protein NS1 has been implicated in viral RNA replication, although its precise role has not been identified. In its native state NS1 exists as a heat labile homodimer that is thought to be required for NS1 function and secretion. However, we have recently identified a cDNA clone of KUN vir...

journal_title：Virology

authors： Hall RA,Khromykh AA,Mackenzie JM,Scherret JH,Khromykh TI,Mackenzie JS

更新日期：1999-11-10 00:00:00

abstract：:The influenza RNA polymerase is known to catalyse three distinct copying activities: (i) transcription of minus-sense virion RNA (vRNA) into mRNA, (ii) transcription of vRNA into full-length complementary RNA (cRNA), and (iii) transcription of cRNA to vRNA. Ever since the discovery of the conserved 13 and 12 long sequ...

journal_title：Virology

authors： Seong BL,Brownlee GG

更新日期：1992-01-01 00:00:00

abstract：:Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...

journal_title：Virology

authors： Beck J,Bartos H,Nassal M

更新日期：1997-01-20 00:00:00

abstract：:Transgenic pigs are currently the most favored potential source of organs for xenotransplantation. Like all mammalian species they all harbor endogenous retroviruses in their genome. These porcine endogenous retroviruses (PERVs) are produced from several primary cells and cell lines and are able to infect human cells....

journal_title：Virology

authors： Tacke SJ,Kurth R,Denner J

更新日期：2000-03-01 00:00:00

abstract：:A method was devised which allows the easy detection of mutations within the ner gene of Mu DNA. This method is based upon the observation that a transcriptional gene A-galK fusion containing the complete ner gene and the cts62 allele does not express the galK gene in an Escherichia coli strain lacking functional inte...

journal_title：Virology

authors： König U,Kirschner P,Schumann W

更新日期：1988-05-01 00:00:00

abstract：:Sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (HN) protein of a 1957 isolate of parainfluenza type 3 virus (PIV3) were produced and used to examine antigenic variation in clinical strains. Analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in...

journal_title：Virology

authors： van Wyke Coelingh KL,Winter C,Murphy BR

更新日期：1985-06-01 00:00:00

abstract：:Following an increase in detection of enterovirus 68 (EV68) in community surveillance of respiratory infections in The Netherlands in 2010, epidemiological and virological analyses were performed to investigate the possible public health impact of EV68 infections. We retrospectively tested specimens collected from acu...

journal_title：Virology

authors： Meijer A,van der Sanden S,Snijders BE,Jaramillo-Gutierrez G,Bont L,van der Ent CK,Overduin P,Jenny SL,Jusic E,van der Avoort HG,Smith GJ,Donker GA,Koopmans MP

更新日期：2012-02-05 00:00:00

abstract：:The vaccinia virus temperature-sensitive mutations Cts6 and Cts9 were mapped by marker rescue and DNA sequencing to the A28 gene. Cts6 and Cts9 contain an identical 2-bp deletion truncating the A28 protein and removing the fourth conserved cysteine near the C-terminus. Cts9 mutant virions produced at 40 degrees C were...

journal_title：Virology

authors： Turner PC,Dilling BP,Prins C,Cresawn SG,Moyer RW,Condit RC

更新日期：2007-09-15 00:00:00

abstract：:Beet necrotic yellow vein virus (BNYVV) has a quadripartite plus-strand RNA genome in which the two smallest genome components, RNA 3 and 4, are not necessary for virus multiplication in leaves. Infectious transcripts of BNYVV RNA 3 and 4 have already been described (V. Ziegler-Graff, S. Bouzoubaa, I. Jupin, H. Guille...

journal_title：Virology

authors： Quillet L,Guilley H,Jonard G,Richards K

更新日期：1989-09-01 00:00:00

abstract：:Tomato strain L of tobacco mosaic virus (TMV-L) induces a hypersensitive response (necrotic local lesions) on tobacco plants with the N' gene. A factor responsible for induction of the hypersensitive response has been mapped to the coat protein gene. We have constructed several mutants which have insertions or deletio...

journal_title：Virology

authors： Saito T,Yamanaka K,Watanabe Y,Takamatsu N,Meshi T,Okada Y

更新日期：1989-11-01 00:00:00

abstract：:Our study reports the occurrence of HIV-1 insertional activation events in vivo. Using a previously described PCR assay, small aliquots of broncho-alveolar lavage (BAL) cells obtained from AIDS patients were analyzed. Nine percent (5/54) of the aliquots contained proviral-host sequence transcripts indicating HIV-1 pro...

journal_title：Virology

authors： Raineri I,Solèr M,Senn HP

更新日期：1995-04-01 00:00:00

abstract：:Early region 1 (E1) of the human adenoviruses has many intriguing properties which have prompted numerous mutational studies to help delineate and characterize the domains responsible for these functions. In mutational analyses being done currently, the E1 region is usually cloned into a bacterial plasmid where it is ...

journal_title：Virology

authors： McGrory WJ,Bautista DS,Graham FL

更新日期：1988-04-01 00:00:00

abstract：:Adsorption and one-step growth experiments performed with blue-green algal virus (BGAV) LPP-1 under normal conditions revealed a slow adsorption rate, in comparison with bacteriophage systems, followed by a 7-hour latent period. The burst size was approximately 100 virus particles per cell. The molecular weight of the...

journal_title：Virology

authors： Goldstein DA,Bendet IJ,Lauffer MA,Smith KM

更新日期：1967-08-01 00:00:00

abstract：:Nipah virus (NV) and Hendra virus (HV) are recently emergent, related viruses that can cause severe disease in humans and animals. The goal of this study was to investigate the immunogenic and functional properties of the fusion (F) and attachment (G) glycoproteins of NV. Vaccination of mice with recombinant vaccinia ...

journal_title：Virology

authors： Tamin A,Harcourt BH,Ksiazek TG,Rollin PE,Bellini WJ,Rota PA

更新日期：2002-04-25 00:00:00

abstract：:We used a mouse nasal model of herpes simplex virus 2 (HSV-2) infection to examine the biological properties of HSV-2 wild-type (wt), TK-negative, and replication-defective strains in vivo. Nasal septa tissue is the major site of wt viral replication post intranasal (i.n.) inoculation. The HSV-2 strain 186 syn(+)-1 wt...

journal_title：Virology

authors： Jones CA,Taylor TJ,Knipe DM

更新日期：2000-12-05 00:00:00

abstract：:Groundnut bud necrosis virus induces necrotic symptoms in different hosts. Previous studies showed reactive oxygen species-mediated programmed cell death (PCD) resulted in necrotic symptoms. Transgenic expression of viral protein NSs mimics viral symptoms. Here, we showed a role for NSs in influencing oxidative burst ...

journal_title：Virology

authors： Singh A,Permar V,Jain RK,Goswami S,Kumar RR,Canto T,Palukaitis P,Praveen S

更新日期：2017-08-01 00:00:00

abstract：:Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...

journal_title：Virology

authors： Volkman LE,Zaal KJ

更新日期：1990-03-01 00:00:00

abstract：:When effector CD4+ T cells carrying integrated HIV-1 proviruses revert back to a resting memory state, the virus can remain silent in those cells for years. Following re-exposure to the nominal antigen or in response to other stimuli (e.g. pro-inflammatory cytokines), these cells can begin to produce virus. Here we de...

journal_title：Virology

authors： Thibault S,Imbeault M,Tardif MR,Tremblay MJ

更新日期：2009-06-20 00:00:00

abstract：:The Hepatitis B virus Precore protein, present in the secretory pathway as the HBeAg precursor, can associate in the cytoplasm with the Core protein to form heterocapsids, likely to favor viral persistence. Core and Precore proteins share their primary sequence except for ten additional aminoacids at the N-terminus of...

journal_title：Virology

authors： Duriez M,Thouard A,Bressanelli S,Rossignol JM,Sitterlin D

更新日期：2014-08-01 00:00:00

abstract：:We have studied the assembly of human immunodeficiency virus (HIV-1) Gag-B-galactosidase (Gag-B-gal; GBG) fusion proteins into HIV particles in the presence of HIV Gag proteins. Release of fusion proteins from cells was measured by assay of media versus cellular B-gal activities and was dependent on co-expression of u...

journal_title：Virology

authors： Wang CT,Stegeman-Olsen J,Zhang Y,Barklis E

更新日期：1994-05-01 00:00:00

abstract：:Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which repres...

journal_title：Virology

authors： Godeny EK,Speicher DW,Brinton MA

更新日期：1990-08-01 00:00:00

abstract：:Hypervariable region 1 (HVR1) proteins of hepatitis C virus (HCV) have been reported to react broadly with sera of patients with HCV infection. However, the variability of the broad reactivity of individual HVR1 proteins has not been elucidated. We assessed the reactivity of 25 different HVR1 proteins (genotype 1b) wi...

journal_title：Virology

authors： Watanabe K,Yoshioka K,Ito H,Ishigami M,Takagi K,Utsunomiya S,Kobayashi M,Kishimoto H,Yano M,Kakumu S

更新日期：1999-11-10 00:00:00

abstract：:Neutralization epitopes of duck hepatitis B virus (DHBV) have been previously mapped within the N-terminal portion of the pre-S protein using monoclonal antibodies. However, the immune response of ducks to this region is not well characterized at the amino acid level. To this end, we have immunized adult Pekin ducks w...

journal_title：Virology

authors： Chassot S,Lambert V,Kay A,Godinot C,Trepo C,Cova L

更新日期：1994-04-01 00:00:00

abstract：:Expansion of CHIKV outbreaks with appearance of novel mutations are reported from many parts of the world. Two novel mutations viz. E1:K211E and E2:V264A in background of E1:226A are recently identified from Aedes aegypti dominated areas of India. In this study, the role of these mutations in modulation of infectivity...

journal_title：Virology

authors： Agarwal A,Sharma AK,Sukumaran D,Parida M,Dash PK

更新日期：2016-10-01 00:00:00

abstract：:To investigate the role of alfalfa mosaic virus coat protein (CP) in genome activation, asymmetric plus-strand RNA accumulation, and cell-to-cell spread of the virus, mutations were made in the CP gene and putative CP binding sites in the 3'-untranslated region (UTR) of RNA 3. Mutants that produced no CP-related pepti...

journal_title：Virology

authors： van der Vossen EA,Neeleman L,Bol JF

更新日期：1994-08-01 00:00:00

abstract：:cDNAs of beet necrotic yellow vein virus RNAs 3 and 4 could be rendered biologically active when they were placed under the control of the cauliflower mosaic virus 35S promoter and polyadenylation signal. Although the 35S in vivo transcripts should have contained up to forty 5' and several hundred 3' nonviral nucleoti...

journal_title：Virology

authors： Commandeur U,Jarausch W,Li Y,Koenig R,Burgermeister W

更新日期：1991-11-01 00:00:00