Abstract:
:Adsorption and one-step growth experiments performed with blue-green algal virus (BGAV) LPP-1 under normal conditions revealed a slow adsorption rate, in comparison with bacteriophage systems, followed by a 7-hour latent period. The burst size was approximately 100 virus particles per cell. The molecular weight of the virus obtained from sedimentation and diffusion measurements was 51 +/- 3 x 10(6) daltons, and the hydration was calculated to be 0.37 g of H2O per gram of virus. Phosphorus analysis indicated a DNA content of 40% w/w, while amino acid analysis revealed all the common amino acids to be present. At reduced magnesium concentrations, the virus breaks apart, liberating its DNA, which then can be separated from the protein by sedimentation in a CsCl density gradient. A protein component was obtained as ghosts. Neutralization experiments with BGAV antiserum established no relationship to bacteriophages T1, T2, or T3.
journal_name
Virologyjournal_title
Virologyauthors
Goldstein DA,Bendet IJ,Lauffer MA,Smith KMdoi
10.1016/0042-6822(67)90036-0subject
Has Abstractpub_date
1967-08-01 00:00:00pages
601-13issue
4eissn
0042-6822issn
1096-0341pii
0042-6822(67)90036-0journal_volume
32pub_type
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